当前蛋白身份：O85465
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差异标签只比较当前检索结果；所有PDB和assembly原始记录仍分别保留。
相关结构差异明细
一行代表一个 PDB 条目中的一个 biological assembly；同一蛋白的多个单体会分别列出。
| PDB 条目 | Assembly / 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与非聚合物 | 实验方法 | 实验环境 | 结构质量 |
|---|---|---|---|---|---|---|---|
| 1A3H ENDOGLUCANASE CEL5A FROM BACILLUS AGARADHERANS AT 1.6A RESOLUTION 提交 1998-01-21 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
30–329（300 aa）
片段：CATALYTIC CORE DOMAIN
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 4.5；pH 4.5
|
分辨率 1.57 Å R-free 0.170 |
| 1E5J ENDOGLUCANASE CEL5A FROM BACILLUS AGARADHAERENS IN THE TETRAGONAL CRYSTAL FORM IN COMPLEX WITH METHYL-4II-S-ALPHA-CELLOBIOSYL-4II-THIO-BETA-CELLOBIOSIDE 提交 2000-07-26 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
27–331（305 aa）
片段：CATALYTIC CORE DOMAIN, RESIDUES 27-331
|
未记录 | CA CALCIUM ION × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 7；PROTEIN (20MGML-1) WAS CRYSTALLISED FROM 28% PEG400 AS PRECIPITANT, 100MM HEPES AT PH 7.0 AS BUFFER AND 200 MM CACL2. THE PROTEIN WAS INCUBATED WITH THE 1MM OF SUBSTRATE FOR AN HOUR PRIOR TO COCRYSTALLISATIOM
|
分辨率 1.85 Å R-free 0.222 |
| 1H11 2-DEOXY-2-FLURO-B-D-CELLOTRIOSYL/ENZYME INTERMEDIATE COMPLEX OF THE ENDOGLUCANASE CEL5A FROM BACILLUS AGARADHEARANS AT 1.08 ANGSTROM RESOLUTION 提交 2002-07-01 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
27–329（303 aa）
片段：CATALYTIC CORE DOMAIN ONLY, RESIDUES 27-329
|
未记录 | SO4 SULFATE ION × 1 GOL GLYCEROL × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 5.5；PROTEIN CONCENTRATION 20MG/ML, 2M AMMONIUM SULPHATE, 25% GLYCEROL AS CRYOPROTECTANT, pH 5.50
|
分辨率 1.08 Å R-free 0.125 |
| 1H2J ENDOGLUCANASE CEL5A IN COMPLEX WITH UNHYDROLYSED AND COVALENTLY LINKED 2,4-DINITROPHENYL-2-DEOXY-2-FLUORO-CELLOBIOSIDE AT 1.15 A RESOLUTION 提交 2002-08-09 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
27–329（303 aa）
片段：CATALYTIC CORE DOMAIN ONLY, RESIDUES 27-329
|
未记录 | DCB 2,4-DINITROPHENYL-2-DEOXY-2-FLUORO-BETA-D-CELLOBIOSIDE × 1 GOL GLYCEROL × 2 SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 4.6；PROTEIN CONCENTRATION 20MG/ML, 2M AMMONIUM SULPHATE, 25% GLYCEROL AS CRYOPROTECTANT, pH 4.60
|
分辨率 1.15 Å R-free 0.136 |
| 1H5V Thiopentasaccharide complex of the endoglucanase Cel5A from Bacillus agaradharens at 1.1 A resolution in the tetragonal crystal form 提交 2001-05-28 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
27–330（304 aa）
片段：CATALYTIC CORE DOMAIN RESIDUES 27-331
|
未记录 | CA CALCIUM ION × 10 NA SODIUM ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 7；PROTEIN (20MGML-1) WAS CRYSTALLISED FROM 28% PEG400 AS PRECIPITANT, 100MM HEPES AT PH 7.0 AS BUFFER AND 200 MM CACL2. THE PROTEIN WAS INCUBATED WITH THE 1MM OF SUBSTRATE FOR AN HOUR PRIOR TO COCRYSTALLISATIOM
|
分辨率 1.10 Å R-free 0.138 |
| 1HF6 ENDOGLUCANASE CEL5A FROM BACILLUS AGARADHAERENS IN THE ORTHORHOMBIC CRYSTAL FORM IN COMPLEX WITH CELLOTRIOSE 提交 2000-11-29 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
27–329（303 aa）
片段：CATALYTIC CORE DOMAIN ONLY
|
未记录 | SO4 SULFATE ION × 1 ACY ACETIC ACID × 1 GOL GLYCEROL × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 4.6；PROTEIN CONCENTRATION 20MG/ML, 2M AMMONIUM SULPHATE, 100MM SODIUM CITRATE PH 5.5, 25% GLYCEROL AS CRYOPROTECTANT
|
分辨率 1.15 Å R-free 0.137 |
| 1OCQ COMPLEX OF THE ENDOGLUCANASE CEL5A FROM BACILLUS AGARADHEARANS AT 1.08 ANGSTROM RESOLUTION with cellobio-derived isofagomine 提交 2003-02-09 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
27–329（303 aa）
片段：CATALYTIC CORE DOMAIN ONLY, RESIDUES 27-329
|
未记录 | IFM 5-HYDROXYMETHYL-3,4-DIHYDROXYPIPERIDINE × 1 BGC beta-D-glucopyranose × 1 GOL GLYCEROL × 2 SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 5.2；PROTEIN WAS USED AT A CONCENTRATION OF 10MG/ML. IT WAS INCUBATED WITH 5MM CELLOBIO-DERIVED ISOFAGOMINE FOR AN HOUR PRIOR CRYSTALLISATION. THE VAPOR DIFFUSION METHOD WAS USED. 1.3 M AMMONIUM SULPHATE WERE USED AS PRECIPITANT. 20 % GLYCEROL WAS ADDED FOR CRYOPROTECTION, pH 5.20
|
分辨率 1.08 Å R-free 0.127 |
| 1W3K ENDOGLUCANASE CEL5A FROM BACILLUS AGARADHAERENS IN COMPLEX WITH CELLOBIO DERIVED-TETRAHYDROOXAZINE 提交 2004-07-16 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
27–329（303 aa）
片段：CATALYTIC CORE DOMAIN, RESIDUES 30-329
|
未记录 | BGC beta-D-glucopyranose × 1 OXZ TETRAHYDROOXAZINE × 1 GOL GLYCEROL × 2 SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 5；PROTEIN CONCENTRATION 20MG/ML, 1.3 M AMMONIUM SULPHATE PH 5.0, 25% GLYCEROL AS CRYOPROTECTANT
|
分辨率 1.20 Å R-free 0.132 |
| 1W3L ENDOGLUCANASE CEL5A FROM BACILLUS AGARADHAERENS IN COMPLEX WITH CELLOTRI DERIVED-TETRAHYDROOXAZINE 提交 2004-07-16 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
27–329（303 aa）
片段：CATALYTIC MODULE, RESIDUES 27-329
|
未记录 | GOL GLYCEROL × 2 SO4 SULFATE ION × 1 OXZ TETRAHYDROOXAZINE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 5；PROTEIN CONCENTRATION 20MG/ML, 1.3 M AMMONIUM SULPHATE PH 5.0, 25% GLYCEROL AS CRYOPROTECTANT
|
分辨率 1.04 Å R-free 0.121 |
| 2A3H CELLOBIOSE COMPLEX OF THE ENDOGLUCANASE CEL5A FROM BACILLUS AGARADHERANS AT 2.0 A RESOLUTION 提交 1998-01-22 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
30–329（300 aa）
片段：CATALYTIC CORE
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 4.5；pH 4.5
|
分辨率 2.00 Å R-free 0.190 |
| 2V38 Family 5 endoglucanase Cel5A from Bacillus agaradhaerens in complex with cellobio-derived noeuromycin 提交 2007-06-13 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
27–331（305 aa）
片段：RESIDUES 27-331
|
未记录 | GOL GLYCEROL × 3 SO4 SULFATE ION × 1 IFL (3S,4R,5R)-3,4-DIHYDROXY-5-(HYDROXYMETHYL)PIPERIDIN-2-ONE × 1 IDR alpha-L-idopyranuronic acid × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
10 MG/ML PROTEIN. 1.5-1.8 M AMMONIUM SULPHATE. 25% GLYCEROL AS CRYO.
|
分辨率 1.50 Å R-free 0.143 |
| 3A3H CELLOTRIOSE COMPLEX OF THE ENDOGLUCANASE CEL5A FROM BACILLUS AGARADHERANS AT 1.6 A RESOLUTION 提交 1998-02-01 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
30–329（300 aa）
片段：CATALYTIC CORE
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 4.5；pH 4.5
|
分辨率 1.64 Å R-free 0.176 |
| 5A3H 2-DEOXY-2-FLURO-B-D-CELLOBIOSYL/ENZYME INTERMEDIATE COMPLEX OF THE ENDOGLUCANASE CEL5A FROM BACILLUS AGARADHEARANS AT 1.8 ANGSTROMS RESOLUTION 提交 1998-07-23 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
27–329（303 aa）
片段：CATALYTIC CORE DOMAIN ONLY
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 5.5；pH 5.5
|
分辨率 1.82 Å R-free 0.186 |
| 6A3H 2-DEOXY-2-FLURO-B-D-CELLOTRIOSYL/ENZYME INTERMEDIATE COMPLEX OF THE ENDOGLUCANASE CEL5A FROM BACILLUS AGARADHEARANS AT 1.6 ANGSTROM RESOLUTION 提交 1998-07-22 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
27–329（303 aa）
片段：CATALYTIC CORE DOMAIN ONLY
|
未记录 | GOL GLYCEROL × 8 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 5.5；pH 5.5
|
分辨率 1.68 Å R-free 0.185 |
| 7A3H NATIVE ENDOGLUCANASE CEL5A CATALYTIC CORE DOMAIN AT 0.95 ANGSTROMS RESOLUTION 提交 1998-08-05 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
27–329（303 aa）
片段：CATALYTIC CORE DOMAIN
|
未记录 | GOL GLYCEROL × 1 EOH ETHANOL × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 5.5；pH 5.5
|
分辨率 0.95 Å R-free 0.130 |