当前蛋白身份：O95631
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差异标签只比较当前检索结果；所有PDB和assembly原始记录仍分别保留。
相关结构差异明细
一行代表一个 PDB 条目中的一个 biological assembly；同一蛋白的多个单体会分别列出。
| PDB 条目 | Assembly / 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与非聚合物 | 实验方法 | 实验环境 | 结构质量 |
|---|---|---|---|---|---|---|---|
| 4URT The crystal structure of a fragment of netrin-1 in complex with FN5- FN6 of DCC 提交 2014-07-02 | Assembly 1 其他组合 异源复合物；蛋白 × 3 PDB 声明：trimeric（3） 与蛋白数一致 |
链 A
39–453（415 aa）
片段：VI AND V DOMAINS, RESIDUES 39-453
|
未记录 | CA CALCIUM ION × 1 CL CHLORIDE ION × 1 SO4 SULFATE ION × 18 GOL GLYCEROL × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 6；0.1M MES PH 6.0, 0.15M AMMONIUM SULFATE AND 15% (W/V) PEG 4000
|
分辨率 3.10 Å R-free 0.265 |
| 6FKQ THE CRYSTAL STRUCTURE OF A FRAGMENT OF NETRIN-1 IN COMPLEX WITH A FRAGMENT OF DRAXIN 提交 2018-01-24 | Assembly 1 其他组合 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
39–453（415 aa）
|
未记录 | CA CALCIUM ION × 1 SO4 SULFATE ION × 10 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 4；298 K；1.6 M AMMONIUM SULPHATE AND 0.1 M
SODIUM CITRATE, AT PH 4 TO PH 5, VAPOR DIFFUSION, TEMPERATURE
298K
|
分辨率 3.07 Å R-free 0.271 |
| 7NDG Cryo-EM structure of the ternary complex between Netrin-1, Neogenin and Repulsive Guidance Molecule B 提交 2021-02-01 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 12 PDB 声明：pentadecameric（15） 待复核 |
链 A
25–453（429 aa）
链 D
25–453（429 aa）
链 G
25–453（429 aa）
|
未记录 | CA CALCIUM ION × 3 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 12 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5；10 mM HEPES pH 7.5, 150 mM NaCl, 2 mM CaCl2, 1 mM sucrose octasulfate, 0.01% NaN3
cryo-EM玻璃化条件
冷冻剂 ETHANE；Lacey carbon grids with 3 nm ultrathin carbon support film were glow discharged for 30 seconds at high RF level using Harrick Plasma Cleaner, model PDC-002-CE, and then 3.5 microl of the sample was pipetted per grid. Excess protein was blotted away for 3 seconds using filter paper (round filter paper for Vitrobot from Agar Scientific, catalogue number 47000-100) and Vitrobot Mark IV (Thermo Fisher Scientific) (relative force -15) at 95-100% humidity. Grids were plunge frozen in liquid ethane.
|
分辨率 5.98 Å |
| 7NE0 Structure of the ternary complex between Netrin-1, Repulsive-Guidance Molecule-B (RGMB) and Neogenin 提交 2021-02-02 | Assembly 1 其他组合 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 A
24–453（430 aa）
|
未记录 | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 CA CALCIUM ION × 1 SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6.5；298.15 K；0.1 M imidazole/MES pH 6.5, 10% (w/v) PEG 8000, 20% (v/v) ethylene glycol, 30 mM sodium nitrate, 30 mM sodium phosphate, 30 mM ammonium sulphate
|
分辨率 3.25 Å R-free 0.263 |
| 7NE1 Structure of the complex between Netrin-1 and its receptor Neogenin 提交 2021-02-02 | Assembly 1 其他组合 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
24–453（430 aa）
|
未记录 | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 CA CALCIUM ION × 1 NO3 NITRATE ION × 3 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；298.15 K；0.2 M ammonium nitrate, 20% w/v PEG 3350, 40 mM potassium/sodium tartrate
|
分辨率 3.15 Å R-free 0.239 |