Current Protein Identity:P00642
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1CKQ PRE-TRANSITION STATE ECO RI ENDONUCLEASE/COGNATE DNA (TCGCGAATTCGCG) COMPLEX Deposited 1999-04-22 | Assembly 1 Protein–DNA Homooligomer;Protein × 2 PDB declaration: tetrameric(4) Consistent with all polymers |
Chain A
1–276(276 aa)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 7.5;pH 7.5, VAPOR DIFFUSION, HANGING DROP
|
Resolution 1.85 Å R-free 0.286 |
| 1CL8 A PRE-TRANSITION STATE ECO RI ENDONUCLEASE/COGNATE DNA (TCGCGAPTTCGCG) COMPLEX WITH DNA BASE ANALOG PURINE (P) Deposited 1999-05-06 | Assembly 1 Protein–DNA Homooligomer;Protein × 2 PDB declaration: tetrameric(4) Consistent with all polymers |
Chain A
1–276(276 aa)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 7.5;pH 7.5, VAPOR DIFFUSION, HANGING DROP
|
Resolution 1.80 Å R-free 0.287 |
| 1ERI X-RAY STRUCTURE OF THE DNA-ECO RI ENDONUCLEASE-DNA RECOGNITION COMPLEX: THE RECOGNITION NETWORK AND THE INTEGRATION OF RECOGNITION AND CLEAVAGE Deposited 1994-05-18 | Assembly 1 Protein–DNA Homooligomer;Protein × 2 PDB declaration: tetrameric(4) Consistent with all polymers |
Chain A
1–276(276 aa)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
277 K;277.00K
|
Resolution 2.50 Å R-free 0.280 |
| 1QC9 THE CRYSTALLOGRAPHIC STRUCTURE OF RESTRICTION ENDONUCLEASE ECO RI AT 3.3 A IN THE ABSENSE OF DNA Deposited 1999-05-18 | Assembly 1 Protein homooligomer Homooligomer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain A
2–277(276 aa)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 3.00 Å R-free 0.295 |
| 1QC9 THE CRYSTALLOGRAPHIC STRUCTURE OF RESTRICTION ENDONUCLEASE ECO RI AT 3.3 A IN THE ABSENSE OF DNA Deposited 1999-05-18 | Assembly 2 Protein homooligomer Homooligomer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain B
2–277(276 aa)
Chain C
2–277(276 aa)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 3.00 Å R-free 0.295 |
| 1QPS THE CRYSTAL STRUCTURE OF A POST-REACTIVE COGNATE DNA-ECO RI COMPLEX AT 2.50 A IN THE PRESENCE OF MN2+ ION Deposited 1999-05-28 | Assembly 1 Protein–DNA Homooligomer;Protein × 2 PDB declaration: hexameric(6) Consistent with all polymers |
Chain A
16–276(261 aa)
Fragment:RESIDUES 17-277
|
Not recorded | MN MANGANESE (II) ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION;pH 7.5;277 K;MN ION WAS SOAKED INTO PRE-REACTIVE PROTEIN-DNA COCRYSTALS AND DNA CLEAVAGE OCCURED IN-CRYSTALLO, pH 7.5, VAPOR
DIFFUSION, temperature 4K
|
Resolution 2.50 Å |
| 1QRH X-RAY STRUCTURE OF THE DNA-ECO RI ENDONUCLEASE COMPLEXES WITH AN R145K MUTATION AT 2.7 A Deposited 1999-06-14 | Assembly 1 Protein–DNA Homooligomer;Protein × 2 PDB declaration: tetrameric(4) Consistent with all polymers |
Chain A
16–276(261 aa)
Fragment:RESIDUES 17-277
|
Mutation:ARG145LYS | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 6.5;277 K;PRECIPITANT: 15% PEG 400, 40 MM BTP, PH 7.1; RESERVOIR: 15% PEG 3350; 40 MM BTP; PH 6.5; DROP:3 UL PROTEIN, 2 UL DNA,
1.5 UL PPT, VAPOR DIFFUSION, SITTING DROP
|
Resolution 2.50 Å |
| 1QRI X-RAY STRUCTURE OF THE DNA-ECO RI ENDONUCLEASE COMPLEXES WITH AN E144D MUTATION AT 2.7 A Deposited 1999-06-14 | Assembly 1 Protein–DNA Homooligomer;Protein × 2 PDB declaration: tetrameric(4) Consistent with all polymers |
Chain A
16–276(261 aa)
Fragment:RESIDUES 17-277
|
Mutation:GLU144ASP | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 8;277 K;PRECIPITANT: 40% PEG 400; 40 MM BTP; PH 8.0; RESERVOIR: 16% PEG 400; 40 MM BTP;
PH 6.5; DROP: 5 UL PROTEIN, 2 UL DNA, 1 UL PPT, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 2.60 Å |
| 2OXV Structure of the A138T promiscuous mutant of the EcoRI restriction endonuclease bound to its cognate recognition site. Deposited 2007-02-21 | Assembly 1 Protein–DNA Homooligomer;Protein × 2 PDB declaration: tetrameric(4) Consistent with all polymers |
Chain A
1–277(277 aa)
|
Mutation:A138T | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 6.5;277.15 K;cryoprotectant solution is 40mM bis-tris Propane, 16% v/v PEG 400, 15% v/v glycerol, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K
|
Resolution 1.95 Å R-free 0.244 |