Current Protein Identity：P00778
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1BOQ PRO REGION C-TERMINUS: PROTEASE ACTIVE SITE INTERACTIONS ARE CRITICAL IN CATALYZING THE FOLDING OF ALPHA-LYTIC PROTEASE Deposited 1998-08-05 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
200–397（198 aa）
|
Mutation：V119I | SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8；pH 8.0
|
Resolution 2.10 Å R-free 0.236 |
| 1GBA ALPHA-LYTIC PROTEASE WITH MET 190 REPLACED BY ALA AND GLY 216 REPLACED BY ALA Deposited 1995-09-06 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
200–397（198 aa）
|
Mutation：M190A, G216A | SO4 SULFATE ION × 2 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.15 Å |
| 1GBB Alpha-lytic protease with met 190 replaced by ALA AND GLY 216 replaced by ALA complex with METHOXYSUCCINYL-ALA-ALA-PRO-ALANINE BORONIC ACID Deposited 1995-09-06 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
200–397（198 aa）
|
Mutation：M190A, G216A | SO4 SULFATE ION × 2 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.15 Å |
| 1GBC ALPHA-LYTIC PROTEASE WITH MET 190 REPLACED BY ALA AND GLY 216 REPLACED BY ALA COMPLEX WITH METHOXYSUCCINYL-ALA-ALA-PRO-LEUCINE BORONIC ACID Deposited 1995-09-06 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
200–397（198 aa）
|
Mutation：M190A, G216A | SO4 SULFATE ION × 2 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.20 Å |
| 1GBD ALPHA-LYTIC PROTEASE WITH MET 190 REPLACED BY ALA AND GLY 216 REPLACED BY ALA COMPLEX WITH METHOXYSUCCINYL-ALA-ALA-PRO-PHENYLALANINE BORONIC ACID Deposited 1995-09-06 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
200–397（198 aa）
|
Mutation：M190A, G216A | SO4 SULFATE ION × 2 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.20 Å |
| 1GBE ALPHA-LYTIC PROTEASE WITH MET 190 REPLACED BY ALA AND GLY 216 REPLACED BY LEU Deposited 1995-09-06 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
200–397（198 aa）
|
Mutation：M190A, G216L | SO4 SULFATE ION × 2 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.30 Å |
| 1GBF ALPHA-LYTIC PROTEASE WITH MET 190 REPLACED BY ALA AND GLY 216 REPLACED BY LEU COMPLEX WITH METHOXYSUCCINYL-ALA-ALA-PRO-ALANINE BORONIC ACID Deposited 1995-09-06 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
200–397（198 aa）
|
Mutation：M190A, G216L | SO4 SULFATE ION × 2 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.15 Å |
| 1GBH ALPHA-LYTIC PROTEASE WITH MET 190 REPLACED BY ALA AND GLY 216 REPLACED BY LEU COMPLEX WITH METHOXYSUCCINYL-ALA-ALA-PRO-LEUCINE BORONIC ACID Deposited 1995-09-06 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
200–397（198 aa）
|
Mutation：M190A, G216L | SO4 SULFATE ION × 2 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.20 Å |
| 1GBI ALPHA-LYTIC PROTEASE WITH MET 190 REPLACED BY ALA AND GLY 216 REPLACED BY LEU COMPLEX WITH METHOXYSUCCINYL-ALA-ALA-PRO-PHENYLALANINE BORONIC ACID Deposited 1995-09-06 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
200–397（198 aa）
|
Mutation：M190A, G216L | SO4 SULFATE ION × 2 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.30 Å |
| 1GBJ ALPHA-LYTIC PROTEASE WITH MET 190 REPLACED BY ALA Deposited 1995-09-06 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
200–397（198 aa）
|
Mutation：M190A | SO4 SULFATE ION × 2 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.00 Å |
| 1GBK ALPHA-LYTIC PROTEASE WITH MET 190 REPLACED BY ALA COMPLEX WITH METHOXYSUCCINYL-ALA-ALA-PRO-ALANINE BORONIC ACID Deposited 1995-09-06 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
200–397（198 aa）
|
Mutation：M190A | SO4 SULFATE ION × 2 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.13 Å |
| 1GBL ALPHA-LYTIC PROTEASE WITH MET 190 REPLACED BY ALA COMPLEX WITH METHOXYSUCCINYL-ALA-ALA-PRO-LEUCINE BORONIC ACID Deposited 1995-09-06 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
200–397（198 aa）
|
Mutation：M190A | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.15 Å |
| 1GBM ALPHA-LYTIC PROTEASE WITH MET 190 REPLACED BY ALA COMPLEX WITH METHOXYSUCCINYL-ALA-ALA-PRO-PHENYLALANINE BORONIC ACID Deposited 1995-09-06 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
200–397（198 aa）
|
Mutation：M190A | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.28 Å |
| 1P01 Serine protease mechanism. structure of an inhibitory complex oF ALPHA-LYTIC Protease and a tightly bound peptide boronic acid Deposited 1989-04-24 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
200–397（198 aa）
|
Not recorded | 0EG N-(tert-butoxycarbonyl)-L-alanyl-N-[(1R)-1-(dihydroxyboranyl)-2-methylpropyl]-L-prolinamide × 1 SO4 SULFATE ION × 1 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.00 Å |
| 1P02 STRUCTURE ANALYSIS OF SPECIFICITY. ALPHA-LYTIC PROTEASE COMPLEXES WITH ANALOGUES OF REACTION INTERMEDIATES Deposited 1989-04-24 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
200–397（198 aa）
|
Not recorded | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.00 Å |
| 1P03 STRUCTURE ANALYSIS OF SPECIFICITY. ALPHA-LYTIC PROTEASE COMPLEXES WITH ANALOGUES OF REACTION INTERMEDIATES Deposited 1989-04-24 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
200–397（198 aa）
|
Not recorded | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.15 Å |
| 1P04 STRUCTURE ANALYSIS OF SPECIFICITY. ALPHA-LYTIC PROTEASE COMPLEXES WITH ANALOGUES OF REACTION INTERMEDIATES Deposited 1989-04-24 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
200–397（198 aa）
|
Not recorded | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.55 Å |
| 1P05 STRUCTURE ANALYSIS OF SPECIFICITY. ALPHA-LYTIC PROTEASE COMPLEXES WITH ANALOGUES OF REACTION INTERMEDIATES Deposited 1989-04-24 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
200–397（198 aa）
|
Not recorded | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.10 Å |
| 1P06 STRUCTURE ANALYSIS OF SPECIFICITY. ALPHA-LYTIC PROTEASE COMPLEXES WITH ANALOGUES OF REACTION INTERMEDIATES Deposited 1989-04-24 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
200–397（198 aa）
|
Not recorded | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.34 Å |
| 1P09 STRUCTURAL PLASTICITY AS A DETERMINANT OF ENZYME SPECIFICITY. CREATING BROADLY SPECIFIC PROTEASES Deposited 1989-04-24 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
200–397（198 aa）
|
Not recorded | SO4 SULFATE ION × 2 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.20 Å |
| 1P10 STRUCTURAL PLASTICITY AS A DETERMINANT OF ENZYME SPECIFICITY. CREATING BROADLY SPECIFIC PROTEASES Deposited 1989-04-24 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
200–397（198 aa）
|
Not recorded | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.25 Å |
| 1P11 CRYSTAL STRUCTURES OF ALPHA-LYTIC PROTEASE COMPLEXES WITH IRREVERSIBLY BOUND PHOSPHONATE ESTERS Deposited 1990-10-26 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain E
200–397（198 aa）
|
Not recorded | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 1.93 Å |
| 1P12 CRYSTAL STRUCTURES OF ALPHA-LYTIC PROTEASE COMPLEXES WITH IRREVERSIBLY BOUND PHOSPHONATE ESTERS Deposited 1990-10-26 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain E
200–397（198 aa）
|
Not recorded | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 1.90 Å |
| 1QQ4 CRYSTAL STRUCTURE OF AN ALPHA-LYTIC PROTEASE MUTANT WITH ACCELERATED FOLDING KINETICS, R102H/G134S Deposited 1999-06-10 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
200–397（198 aa）
Fragment：RESIDUES 1-198
|
Mutation：R102H,G134S | SO4 SULFATE ION × 5 GOL GLYCEROL × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.14；298 K；lithium sulfate, tris-sulfate, pH 5.14, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.20 Å R-free 0.174 |
| 1QRW CRYSTAL STRUCTURE OF AN ALPHA-LYTIC PROTEASE MUTANT WITH ACCELERATED FOLDING KINETICS, R102H/G134S, PH 8 Deposited 1999-06-16 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
200–397（198 aa）
Fragment：MATURE PROTEASE DOMAIN, RESIDUES 1-198
|
Mutation：R102H,G134S | SO4 SULFATE ION × 4 GOL GLYCEROL × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；298 K；LITHIUM SULFATE, TRIS-SULFATE, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.20 Å R-free 0.181 |
| 1QRX CRYSTAL STRUCTURE OF WILD-TYPE ALPHA-LYTIC PROTEASE AT 1.6 A, PH 5.14 Deposited 1999-06-16 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
200–397（198 aa）
Fragment：MATURE PROTEASE DOMAIN, RESIDUES 1-198
|
Not recorded | SO4 SULFATE ION × 3 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.14；298 K；LITHIUM SULFATE, TRIS-SULFATE, pH 5.14, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.60 Å R-free 0.169 |
| 1SSX 0.83A resolution crystal structure of alpha-lytic protease at pH 8 Deposited 2004-03-24 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
200–397（198 aa）
Fragment：Mature protease domain (residues 200-397)
|
Not recorded | SO4 SULFATE ION × 4 GOL GLYCEROL × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；298 K；1.3M lithium sulfate, 0.02M Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 0.83 Å R-free 0.099 |
| 1TAL ALPHA-LYTIC PROTEASE AT 120 K (SINGLE STRUCTURE MODEL) Deposited 1996-10-30 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
200–397（198 aa）
|
Not recorded | SO4 SULFATE ION × 3 TAM TRIS(HYDROXYETHYL)AMINOMETHANE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8；pH 8.0
|
Resolution 1.50 Å R-free 0.192 |
| 2ALP REFINED STRUCTURE OF ALPHA-LYTIC PROTEASE AT 1.7 ANGSTROMS RESOLUTION. ANALYSIS OF HYDROGEN BONDING AND SOLVENT STRUCTURE Deposited 1985-03-07 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
200–397（198 aa）
|
Not recorded | SO4 SULFATE ION × 2 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 1.70 Å |
| 2H5C 0.82A resolution crystal structure of alpha-lytic protease at pH 5 Deposited 2006-05-25 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
200–397（198 aa）
Fragment：MATURE PROTEASE DOMAIN (RESIDUES 200-397)
|
Not recorded | SO4 SULFATE ION × 11 GOL GLYCEROL × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 4.25；298 K；1.3M LITHIUM SULFATE, 0.02M TRIS, PH 4.25, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K
|
Resolution 0.82 Å R-free 0.093 |
| 2H5D 0.9A resolution crystal structure of alpha-lytic protease complexed with a transition state analogue, MeOSuc-Ala-Ala-Pro-Val boronic acid Deposited 2006-05-25 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
200–397（198 aa）
Fragment：MATURE PROTEASE DOMAIN (RESIDUES 200-397)
|
Not recorded | SO4 SULFATE ION × 8 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8；298 K；1.3M LITHIUM SULFATE, 0.02M TRIS, PH 8.0, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K, pH 8.00
|
Resolution 0.90 Å R-free 0.091 |
| 2LPR STRUCTURAL BASIS FOR BROAD SPECIFICITY IN ALPHA-LYTIC PROTEASE MUTANTS Deposited 1991-08-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
200–397（198 aa）
|
Not recorded | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.25 Å |
| 2PRO PRO REGION OF ALPHA-LYTIC PROTEASE Deposited 1998-08-20 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
34–199（166 aa）
Fragment：PRO REGION
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8；pH 8.0
|
Resolution 3.00 Å R-free 0.342 |
| 2PRO PRO REGION OF ALPHA-LYTIC PROTEASE Deposited 1998-08-20 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
34–199（166 aa）
Fragment：PRO REGION
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8；pH 8.0
|
Resolution 3.00 Å R-free 0.342 |
| 2PRO PRO REGION OF ALPHA-LYTIC PROTEASE Deposited 1998-08-20 | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
34–199（166 aa）
Fragment：PRO REGION
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8；pH 8.0
|
Resolution 3.00 Å R-free 0.342 |
| 2PRO PRO REGION OF ALPHA-LYTIC PROTEASE Deposited 1998-08-20 | Assembly 4 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
34–199（166 aa）
Fragment：PRO REGION
Chain B
34–199（166 aa）
Fragment：PRO REGION
Chain C
34–199（166 aa）
Fragment：PRO REGION
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8；pH 8.0
|
Resolution 3.00 Å R-free 0.342 |
| 2ULL MULTIPLE CONFORMATION STRUCTURE OF ALPHA-LYTIC PROTEASE AT 120 K Deposited 1996-11-26 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
200–397（198 aa）
|
Not recorded | SO4 SULFATE ION × 3 TAM TRIS(HYDROXYETHYL)AMINOMETHANE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8；pH 8.0
|
Resolution 1.50 Å R-free 0.192 |
| 3LPR STRUCTURAL BASIS FOR BROAD SPECIFICITY IN ALPHA-LYTIC PROTEASE MUTANTS Deposited 1991-08-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
200–397（198 aa）
|
Not recorded | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.15 Å |
| 3M7T Crystal Structure of Alpha-Lytic Protease SB2+3 E8A/R105S Mutant Deposited 2010-03-17 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
200–397（198 aa）
Fragment：MATURE PROTEASE DOMAIN (RESIDUES 1-198)
|
Mutation：E8A, R105S | GOL GLYCEROL × 1 SO4 SULFATE ION × 5 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；1.3 M lithium sulfate, 20 mM Tris sulfate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.55 Å R-free 0.202 |
| 3M7U Crystal Structure of Alpha-Lytic Protease SB1+2 R64A/E182Q Mutant Deposited 2010-03-17 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
200–397（198 aa）
Fragment：MATURE PROTEASE DOMAIN (RESIDUES 1-198)
|
Mutation：R64A, E182Q | SO4 SULFATE ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；1.3 M lithium sulfate, 20 mM Tris sulfate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.05 Å R-free 0.164 |
| 3PRO ALPHA-LYTIC PROTEASE COMPLEXED WITH C-TERMINAL TRUNCATED PRO REGION Deposited 1998-08-26 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
200–397（198 aa）
Fragment：MATURE PROTEASE
Chain C
34–199（166 aa）
Fragment：PRO REGION
|
Mutation：M158A | AES 4-(2-AMINOETHYL)BENZENESULFONYL FLUORIDE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.25；pH 6.25
|
Resolution 1.80 Å R-free 0.230 |
| 3PRO ALPHA-LYTIC PROTEASE COMPLEXED WITH C-TERMINAL TRUNCATED PRO REGION Deposited 1998-08-26 | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
200–397（198 aa）
Fragment：MATURE PROTEASE
Chain D
34–199（166 aa）
Fragment：PRO REGION
|
Mutation：M158A | AES 4-(2-AMINOETHYL)BENZENESULFONYL FLUORIDE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.25；pH 6.25
|
Resolution 1.80 Å R-free 0.230 |
| 3QGJ 1.3A Structure of alpha-Lytic Protease Bound to Ac-AlaAlaPro-Alanal Deposited 2011-01-24 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
200–397（198 aa）
Fragment：residues 200-397
|
Not recorded | EDO 1,2-ETHANEDIOL × 3 SO4 SULFATE ION × 4 TFA trifluoroacetic acid × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 4.8；277 K；1.2M Li2SO4, 20mM Tris-SO4, pH 4.8, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 1.30 Å R-free 0.192 |
| 3QGJ 1.3A Structure of alpha-Lytic Protease Bound to Ac-AlaAlaPro-Alanal Deposited 2011-01-24 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
200–397（198 aa）
Fragment：residues 200-397
|
Not recorded | EDO 1,2-ETHANEDIOL × 2 SO4 SULFATE ION × 3 TFA trifluoroacetic acid × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 4.8；277 K；1.2M Li2SO4, 20mM Tris-SO4, pH 4.8, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 1.30 Å R-free 0.192 |
| 3QGJ 1.3A Structure of alpha-Lytic Protease Bound to Ac-AlaAlaPro-Alanal Deposited 2011-01-24 | Assembly 3 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain A
200–397（198 aa）
Fragment：residues 200-397
Chain C
200–397（198 aa）
Fragment：residues 200-397
|
Not recorded | EDO 1,2-ETHANEDIOL × 10 SO4 SULFATE ION × 14 TFA trifluoroacetic acid × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 4.8；277 K；1.2M Li2SO4, 20mM Tris-SO4, pH 4.8, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 1.30 Å R-free 0.192 |
| 3URC T181G mutant of alpha-Lytic Protease Deposited 2011-11-22 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
200–397（198 aa）
|
Mutation：T181G | SO4 SULFATE ION × 2 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；1.3 M lithium sulfate and 20 mM Tris sulfate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.10 Å R-free 0.136 |
| 3URD T181A mutant of alpha-Lytic Protease Deposited 2011-11-22 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
200–397（198 aa）
Fragment：UNP residues 200-397
|
Mutation：T132A | SO4 SULFATE ION × 5 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；1.3 M lithium sulfate and 20 mM Tris sulfate, pH 8.0
, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.08 Å R-free 0.142 |
| 3URE Repack mutant (T181I, W199L, Q210I) of alpha-Lytic Protease Deposited 2011-11-22 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
200–397（198 aa）
|
Mutation：T181I, W199L, Q210I | SO4 SULFATE ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；1.3 M lithium sulfate, 10 mM CuCl2, and 20 mM Tris sulfate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.49 Å R-free 0.170 |
| 3URE Repack mutant (T181I, W199L, Q210I) of alpha-Lytic Protease Deposited 2011-11-22 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
200–397（198 aa）
|
Mutation：T181I, W199L, Q210I | SO4 SULFATE ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；1.3 M lithium sulfate, 10 mM CuCl2, and 20 mM Tris sulfate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.49 Å R-free 0.170 |
| 4PRO ALPHA-LYTIC PROTEASE COMPLEXED WITH PRO REGION Deposited 1998-10-01 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
200–397（198 aa）
Fragment：CHAIN A, B, MATURE PROTEASE. CHAIN C, D, PRO REGION
Chain C
34–199（166 aa）
Fragment：CHAIN A, B, MATURE PROTEASE. CHAIN C, D, PRO REGION
|
Mutation：CHAIN A, B, M158A Mutation：CHAIN A, B, M158A | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5；pH 6.5
|
Resolution 2.40 Å R-free 0.238 |
| 4PRO ALPHA-LYTIC PROTEASE COMPLEXED WITH PRO REGION Deposited 1998-10-01 | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
200–397（198 aa）
Fragment：CHAIN A, B, MATURE PROTEASE. CHAIN C, D, PRO REGION
Chain D
34–199（166 aa）
Fragment：CHAIN A, B, MATURE PROTEASE. CHAIN C, D, PRO REGION
|
Mutation：CHAIN A, B, M158A Mutation：CHAIN A, B, M158A | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5；pH 6.5
|
Resolution 2.40 Å R-free 0.238 |
| 5LPR STRUCTURAL BASIS FOR BROAD SPECIFICITY IN ALPHA-LYTIC PROTEASE MUTANTS Deposited 1991-08-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
200–397（198 aa）
|
Not recorded | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.13 Å |
| 5WOT NMR solution structure of a-lytic protease using two 4D-spectra Deposited 2017-08-03 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
200–397（198 aa）
Fragment：residues 200-397
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 4；298 K；Ionic strength (raw mmCIF value) 50；Pressure 1
NMR sample composition
2.0 mM [U-13C; U-15N] alpha-lytic protease protein, 92% H2O/8% D2O | 92% H2O/8% D2O
|
Resolution not provided |
| 6LPR STRUCTURAL BASIS FOR BROAD SPECIFICITY IN ALPHA-LYTIC PROTEASE MUTANTS Deposited 1991-08-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
200–397（198 aa）
|
Not recorded | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.10 Å |
| 7LPR STRUCTURAL BASIS FOR BROAD SPECIFICITY IN ALPHA-LYTIC PROTEASE MUTANTS Deposited 1991-08-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
200–397（198 aa）
|
Not recorded | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.05 Å |
| 8LPR STRUCTURAL BASIS FOR BROAD SPECIFICITY IN ALPHA-LYTIC PROTEASE MUTANTS Deposited 1991-08-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
200–397（198 aa）
|
Not recorded | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.25 Å |
| 9LPR STRUCTURAL BASIS FOR BROAD SPECIFICITY IN ALPHA-LYTIC PROTEASE MUTANTS Deposited 1991-08-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
200–397（198 aa）
|
Not recorded | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.20 Å |