当前蛋白身份：P03689
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差异标签只比较当前检索结果；所有PDB和assembly原始记录仍分别保留。
相关结构差异明细
一行代表一个 PDB 条目中的一个 biological assembly；同一蛋白的多个单体会分别列出。
| PDB 条目 | Assembly / 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与非聚合物 | 实验方法 | 实验环境 | 结构质量 |
|---|---|---|---|---|---|---|---|
| 6BBM Mechanisms of Opening and Closing of the Bacterial Replicative Helicase: The DnaB Helicase and Lambda P Helicase Loader Complex 提交 2017-10-18 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 11 PDB 声明：undecameric（11） 与蛋白数一致 |
链 V
1–107（107 aa）
链 W
1–107（107 aa）
链 X
1–107（107 aa）
链 Y
1–107（107 aa）
链 Z
1–107（107 aa）
|
未记录 | ADP ADENOSINE-5'-DIPHOSPHATE × 5 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5；Concentrated BP sample (18mg/mL) was diluted with freshly prepared buffer to desired concentration (~1.5 micromolar) for grid preparation.
cryo-EM玻璃化条件
冷冻剂 ETHANE；3uL of sample was adhered to a fresh plasma cleaned grid and allowed to adsorb for 30 seconds, blotted for 3 seconds with a blot force of 4 and plunge frozen into liquid nitrogen-cooled ethane.
|
分辨率 4.10 Å |
| 8V9S Distinct Quaternary States, Intermediates, and Autoinhibition During Loading of the DnaB-Replicative Helicase by the Phage Lambda P Helicase Loader 提交 2023-12-09 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
105–210（106 aa）
片段：residues 105 to 210
|
非标准单体：是（mmCIF未提供具体位点） | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；298.15 K；Crystals were prepared using the sitting drop vapor diffusion method by mixing either 0.1, or 0.2, or 0.4 uL of the protein solution and 0.2 uL of a series of commercially available crystallization screens (Qiagen). Optimized crystals for X-ray diffraction were grown at room temperature in a buffer consisting of 3 M to 4 M NaCl with 0.1 M HEPES-NaOH, pH 7 to 8 and required 7 days to form.
|
分辨率 1.86 Å R-free 0.244 |
| 8V9T Ecoli DnaB helicase and Phage Lambda loader P with ADP-Mg in a 6:5 stoichiometry ratio 提交 2023-12-09 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 11 PDB 声明：undecameric（11） 与蛋白数一致 |
链 V
1–233（233 aa）
链 W
1–233（233 aa）
链 X
1–233（233 aa）
链 Y
1–233（233 aa）
链 Z
1–233（233 aa）
|
突变：K2E 突变：K2E 突变：K2E 突变：K2E 突变：K2E | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5；20 mM Na-HEPES pH 7.5, 450mM NaCl, 2mM DTT, 0.5mM MgCl2, 0.2mM ATP, 0.25% Glycerol
cryo-EM玻璃化条件
冷冻剂 ETHANE；Protein BP (1.5 uM) and DNA (1.875 uM) was mixed in a 1.25 molar excess. 3uL of the sample was added to a plasma-cleaned grid at 4 degrees celsius, 100 percent humidity, blot force 4, blot time 4s, wait time 30s, total blots 1, and plunge-frozen into liquid nitrogen-cooled ethane.
|
分辨率 2.84 Å |
| 9OA1 Ecoli DnaB helicase and Phage Lambda loader P with ADP-Mg in a 6:5 stoichiometry ratio. 提交 2025-04-18 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 11 PDB 声明：undecameric（11） 与蛋白数一致 |
链 V
1–233（233 aa）
链 W
1–233（233 aa）
链 X
1–233（233 aa）
链 Y
1–233（233 aa）
链 Z
1–233（233 aa）
|
突变：K2E 突变：K2E 突变：K2E 突变：K2E 突变：K2E | ADP ADENOSINE-5'-DIPHOSPHATE × 6 MG MAGNESIUM ION × 6 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5；20 mM Na-HEPES pH 7.5, 450mM NaCl, 2mM DTT, 0.5mM MgCl2, 0.2mM ATP, 0.25% Glycerol
cryo-EM玻璃化条件
冷冻剂 ETHANE；Protein BP (1.5 uM) and DNA (1.875 uM) was mixed in a 1.25 molar excess. 3uL of the sample was added to a plasma-cleaned grid at 4 degrees celsius, 100 percent humidity, blot force 4, blot time 4s, wait time 30s, total blots 1, and plunge-frozen into liquid nitrogen-cooled ethane.
|
分辨率 2.66 Å |
| 9OA2 Ecoli DnaB helicase and Phage Lambda loader P with ADP-Mg in a 6:6 stoichiometry ratio. 提交 2025-04-18 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 12 PDB 声明：dodecameric（12） 与蛋白数一致 |
链 U
1–233（233 aa）
链 V
1–233（233 aa）
链 W
1–233（233 aa）
链 X
1–233（233 aa）
链 Y
1–233（233 aa）
链 Z
1–233（233 aa）
|
突变：K2E 突变：K2E 突变：K2E 突变：K2E 突变：K2E 突变：K2E | ADP ADENOSINE-5'-DIPHOSPHATE × 6 MG MAGNESIUM ION × 6 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5；20 mM Na-HEPES pH 7.5, 450mM NaCl, 2mM DTT, 0.5mM MgCl2, 0.2mM ATP, 0.25% Glycerol
cryo-EM玻璃化条件
冷冻剂 ETHANE；Protein BP (1.5 uM), ssDNA (1.875 uM), and LO-156-299-NHis (2.25 uM) was mixed at 1.25 and 1.5 molar excess, respectively. 3uL of the sample was added to a plasma-cleaned grid at 4 degrees celsius, 100 percent humidity, blot force 4, blot time 4s, wait time 30s, total blots 1, and plunge-frozen into liquid nitrogen-cooled ethane.
|
分辨率 3.85 Å |