Current Protein Identity：P03689
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 6BBM Mechanisms of Opening and Closing of the Bacterial Replicative Helicase: The DnaB Helicase and Lambda P Helicase Loader Complex Deposited 2017-10-18 | Assembly 1 Protein heterocomplex Heteromer；Protein × 11 PDB declaration: undecameric（11） Consistent with protein count |
Chain V
1–107（107 aa）
Chain W
1–107（107 aa）
Chain X
1–107（107 aa）
Chain Y
1–107（107 aa）
Chain Z
1–107（107 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；Concentrated BP sample (18mg/mL) was diluted with freshly prepared buffer to desired concentration (~1.5 micromolar) for grid preparation.
cryo-EM vitrification conditions
Cryogen ETHANE；3uL of sample was adhered to a fresh plasma cleaned grid and allowed to adsorb for 30 seconds, blotted for 3 seconds with a blot force of 4 and plunge frozen into liquid nitrogen-cooled ethane.
|
Resolution 4.10 Å |
| 8V9S Distinct Quaternary States, Intermediates, and Autoinhibition During Loading of the DnaB-Replicative Helicase by the Phage Lambda P Helicase Loader Deposited 2023-12-09 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
105–210（106 aa）
Fragment：residues 105 to 210
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298.15 K；Crystals were prepared using the sitting drop vapor diffusion method by mixing either 0.1, or 0.2, or 0.4 uL of the protein solution and 0.2 uL of a series of commercially available crystallization screens (Qiagen). Optimized crystals for X-ray diffraction were grown at room temperature in a buffer consisting of 3 M to 4 M NaCl with 0.1 M HEPES-NaOH, pH 7 to 8 and required 7 days to form.
|
Resolution 1.86 Å R-free 0.244 |
| 8V9T Ecoli DnaB helicase and Phage Lambda loader P with ADP-Mg in a 6:5 stoichiometry ratio Deposited 2023-12-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 11 PDB declaration: undecameric（11） Consistent with protein count |
Chain V
1–233（233 aa）
Chain W
1–233（233 aa）
Chain X
1–233（233 aa）
Chain Y
1–233（233 aa）
Chain Z
1–233（233 aa）
|
Mutation：K2E Mutation：K2E Mutation：K2E Mutation：K2E Mutation：K2E | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；20 mM Na-HEPES pH 7.5, 450mM NaCl, 2mM DTT, 0.5mM MgCl2, 0.2mM ATP, 0.25% Glycerol
cryo-EM vitrification conditions
Cryogen ETHANE；Protein BP (1.5 uM) and DNA (1.875 uM) was mixed in a 1.25 molar excess. 3uL of the sample was added to a plasma-cleaned grid at 4 degrees celsius, 100 percent humidity, blot force 4, blot time 4s, wait time 30s, total blots 1, and plunge-frozen into liquid nitrogen-cooled ethane.
|
Resolution 2.84 Å |
| 9OA1 Ecoli DnaB helicase and Phage Lambda loader P with ADP-Mg in a 6:5 stoichiometry ratio. Deposited 2025-04-18 | Assembly 1 Protein heterocomplex Heteromer；Protein × 11 PDB declaration: undecameric（11） Consistent with protein count |
Chain V
1–233（233 aa）
Chain W
1–233（233 aa）
Chain X
1–233（233 aa）
Chain Y
1–233（233 aa）
Chain Z
1–233（233 aa）
|
Mutation：K2E Mutation：K2E Mutation：K2E Mutation：K2E Mutation：K2E | ADP ADENOSINE-5'-DIPHOSPHATE × 6 MG MAGNESIUM ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；20 mM Na-HEPES pH 7.5, 450mM NaCl, 2mM DTT, 0.5mM MgCl2, 0.2mM ATP, 0.25% Glycerol
cryo-EM vitrification conditions
Cryogen ETHANE；Protein BP (1.5 uM) and DNA (1.875 uM) was mixed in a 1.25 molar excess. 3uL of the sample was added to a plasma-cleaned grid at 4 degrees celsius, 100 percent humidity, blot force 4, blot time 4s, wait time 30s, total blots 1, and plunge-frozen into liquid nitrogen-cooled ethane.
|
Resolution 2.66 Å |
| 9OA2 Ecoli DnaB helicase and Phage Lambda loader P with ADP-Mg in a 6:6 stoichiometry ratio. Deposited 2025-04-18 | Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric（12） Consistent with protein count |
Chain U
1–233（233 aa）
Chain V
1–233（233 aa）
Chain W
1–233（233 aa）
Chain X
1–233（233 aa）
Chain Y
1–233（233 aa）
Chain Z
1–233（233 aa）
|
Mutation：K2E Mutation：K2E Mutation：K2E Mutation：K2E Mutation：K2E Mutation：K2E | ADP ADENOSINE-5'-DIPHOSPHATE × 6 MG MAGNESIUM ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；20 mM Na-HEPES pH 7.5, 450mM NaCl, 2mM DTT, 0.5mM MgCl2, 0.2mM ATP, 0.25% Glycerol
cryo-EM vitrification conditions
Cryogen ETHANE；Protein BP (1.5 uM), ssDNA (1.875 uM), and LO-156-299-NHis (2.25 uM) was mixed at 1.25 and 1.5 molar excess, respectively. 3uL of the sample was added to a plasma-cleaned grid at 4 degrees celsius, 100 percent humidity, blot force 4, blot time 4s, wait time 30s, total blots 1, and plunge-frozen into liquid nitrogen-cooled ethane.
|
Resolution 3.85 Å |