Current Protein Identity:P05095
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1G8X STRUCTURE OF A GENETICALLY ENGINEERED MOLECULAR MOTOR Deposited 2000-11-21 | Assembly 1 Insufficient information Homooligomer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain A
266–503(238 aa)
Fragment:MYOSIN II HEAVY CHAIN, MOTOR DOMAIN RESIDUES 1-761, AND ALPHA-ACTININ 3, REPEATS 1 AND 2 RESIDUES 765-1002
Chain B
266–503(238 aa)
Fragment:MYOSIN II HEAVY CHAIN, MOTOR DOMAIN RESIDUES 1-761, AND ALPHA-ACTININ 3, REPEATS 1 AND 2 RESIDUES 765-1002
|
Mutation:ARG238GLU Mutation:ARG238GLU | MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 7.2;280 K;12% PEGM 5000; 170 mM NaCl; 50 mM HEPES (NaOH) pH 7.2; 5 mM MgCl2; 5 mM DTT; 0.5 mM EGTA; and 2% 2-methyl-1,3-propanediol, at 280 K, VAPOR DIFFUSION, SITTING DROP
|
Resolution 2.80 Å R-free 0.290 |
| 4PD3 Crystal Structure of Rigor-Like Human Nonmuscle Myosin-2B Deposited 2014-04-17 | Assembly 1 Insufficient information Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
265–502(238 aa)
Fragment:UNP P35580 residues 1-782, UNP P05095 residues 265-502
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
EVAPORATION;pH 7.5;291.15 K;10% PEG-6000, 0.1 M Tris pH 7.5
|
Resolution 2.84 Å R-free 0.288 |
| 4PD3 Crystal Structure of Rigor-Like Human Nonmuscle Myosin-2B Deposited 2014-04-17 | Assembly 2 Insufficient information Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain B
265–502(238 aa)
Fragment:UNP P35580 residues 1-782, UNP P05095 residues 265-502
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
EVAPORATION;pH 7.5;291.15 K;10% PEG-6000, 0.1 M Tris pH 7.5
|
Resolution 2.84 Å R-free 0.288 |
| 5I4E Crystal Structure of Human Nonmuscle Myosin 2C motor domain Deposited 2016-02-11 | Assembly 1 Insufficient information Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
265–489(225 aa)
Fragment:UNP residues 265-489,UNP residues 265-489
|
Not recorded | AOV ADP ORTHOVANADATE × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;281.15 K;TRIS PH 8.2, PEG 5K MME, MPD, NACL.
|
Resolution 2.25 Å R-free 0.241 |
| 5JLH Cryo-EM structure of a human cytoplasmic actomyosin complex at near-atomic resolution Deposited 2016-04-27 | Assembly 1 Insufficient information Heteromer;Protein × 11 PDB declaration: undecameric(11) Consistent with protein count |
Chain F
265–502(238 aa)
Chain G
265–502(238 aa)
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5;5 mM Tris-HCl pH 7.5, 1 mM DTT, 100 mM KCl, and 2 mM MgCl2
cryo-EM vitrification conditions
Cryogen ETHANE;Sample (2 uL of F-actin-tropomyosin solution) was applied to a glow-discharged holey carbon grid, incubated for 20 s and manually blotted from the backside for less than a second with filter paper. Afterwards 1.5 uL of myosin solution (3 uM without nucleotide) were added directly on the grid, incubated for 10 s and then manually blotted for 5 s from the backside with filter paper.
|
Resolution 3.90 Å |
| 9IHL Crystal Structure of the Human Nonmuscle Myosin 2A Motor Domain Deposited 2025-02-21 | Assembly 1 Insufficient information Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
265–502(238 aa)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;291 K;16% PEG3350,
240 mM Sodium thiocyanate
|
Resolution 2.02 Å R-free 0.259 |