当前蛋白身份：P05102
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差异标签只比较当前检索结果；所有PDB和assembly原始记录仍分别保留。
相关结构差异明细
一行代表一个 PDB 条目中的一个 biological assembly；同一蛋白的多个单体会分别列出。
| PDB 条目 | Assembly / 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与非聚合物 | 实验方法 | 实验环境 | 结构质量 |
|---|---|---|---|---|---|---|---|
| 10MH TERNARY STRUCTURE OF HHAI METHYLTRANSFERASE WITH ADOHCY AND HEMIMETHYLATED DNA CONTAINING 5,6-DIHYDRO-5-AZACYTOSINE AT THE TARGET 提交 1998-08-10 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–327（327 aa）
|
未记录 | SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 6.5；10% (W/V) PEG 8000 50 MM CALCIUM ACETATE 50 MM SODIUM CACODYLATE PH 6.5
|
分辨率 2.55 Å |
| 1FJX STRUCTURE OF TERNARY COMPLEX OF HHAI METHYLTRANSFERASE MUTANT (T250G) IN COMPLEX WITH DNA AND ADOHCY 提交 2000-08-08 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–327（327 aa）
|
突变：T250G | SO4 SULFATE ION × 2 SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 5.6；289 K；2.7 M ammonium sulfate 20% (w/v) glucose 50 mM sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 16K
|
分辨率 2.26 Å R-free 0.283 |
| 1HMY CRYSTAL STRUCTURE OF THE HHAI DNA METHYLTRANSFERASE COMPLEXED WITH S-ADENOSYL-L-METHIONINE 提交 1993-08-05 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–327（327 aa）
|
未记录 | SAM S-ADENOSYLMETHIONINE × 1 | X-RAY DIFFRACTION | mmCIF 未提供已读取条件 | 分辨率 2.50 Å |
| 1M0E ZEBULARINE: A NOVEL DNA METHYLATION INHIBITOR THAT FORMS A COVALENT COMPLEX WITH DNA METHYLTRANSFERASE 提交 2002-06-12 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–327（327 aa）
|
未记录 | SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.5；289 K；PEG 8000, Ca acetate, sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
分辨率 2.50 Å R-free 0.243 |
| 1MHT COVALENT TERNARY STRUCTURE OF HHAI METHYLTRANSFERASE, DNA AND S-ADENOSYL-L-HOMOCYSTEINE 提交 1994-12-08 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–327（327 aa）
|
未记录 | SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.25；289 K；BIS-TRIS-PROPANE_HCL, NACL, EDTA, pH 7.25, VAPOR DIFFUSION, HANGING DROP, temperature 289.00K
|
分辨率 2.60 Å |
| 1SKM HhaI methyltransferase in complex with DNA containing an abasic south carbocyclic sugar at its target site 提交 2004-03-05 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–327（327 aa）
|
未记录 | SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.5；289 K；PEG 6000, sodium cacodylate, magnesium acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
分辨率 2.20 Å R-free 0.221 |
| 1SVU Structure of the Q237W mutant of HhaI DNA methyltransferase: an insight into protein-protein interactions 提交 2004-03-30 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–327（327 aa）
|
突变：Q237W | SO4 SULFATE ION × 2 SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 UNX UNKNOWN LIGAND × 8 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 5.8；289 K；ammonium sulfate, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
分辨率 2.66 Å R-free 0.266 |
| 1SVU Structure of the Q237W mutant of HhaI DNA methyltransferase: an insight into protein-protein interactions 提交 2004-03-30 | Assembly 2 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
1–327（327 aa）
|
突变：Q237W | SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 UNX UNKNOWN LIGAND × 9 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 5.8；289 K；ammonium sulfate, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
分辨率 2.66 Å R-free 0.266 |
| 2C7O HhaI DNA methyltransferase complex with 13mer oligonucleotide containing 2-aminopurine adjacent to the target base (PCGC:GMGC) and SAH 提交 2005-11-25 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–327（327 aa）
|
未记录 | SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 SO4 SULFATE ION × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 5.6；50 MM NA CITRATE PH 5.6, 1.6 M AMMONIUM SULFATE, 5 % GLUCOSE.
|
分辨率 1.90 Å R-free 0.215 |
| 2C7P HhaI DNA methyltransferase complex with oligonucleotide containing 2- aminopurine opposite to the target base (GCGC:GMPC) and SAH 提交 2005-11-25 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–327（327 aa）
|
未记录 | SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 SO4 SULFATE ION × 5 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 CIT CITRIC ACID × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 5.6；50 MM NA CITRATE PH 5.6, 1.4 M AMMONIUM SULFATE
|
分辨率 1.70 Å R-free 0.220 |
| 2C7Q HhaI DNA methyltransferase complex with oligonucleotide containing 2- aminopurine outside the recognition sequence (paired with G) and SAH 提交 2005-11-27 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–327（327 aa）
|
未记录 | SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 SO4 SULFATE ION × 3 CIT CITRIC ACID × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 5.6；50 MM NA CITRATE PH 5.6, 1.6 M AMMONIUM SULFATE
|
分辨率 1.85 Å R-free 0.210 |
| 2C7R HhaI DNA methyltransferase (T250G mutant) complex with oligonucleotide containing 2-aminopurine as a target base (GPGC:GMGC) and SAH 提交 2005-11-27 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–327（327 aa）
|
突变：YES | SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 SO4 SULFATE ION × 7 GOL GLYCEROL × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 5.6；50 MM NA CITRATE PH 5.6, 1.7 M AMMONIUM SULFATE, 5 % GLUCOSE
|
分辨率 1.90 Å R-free 0.217 |
| 2HMY BINARY COMPLEX OF HHAI METHYLTRANSFERASE WITH ADOMET FORMED IN THE PRESENCE OF A SHORT NONPSECIFIC DNA OLIGONUCLEOTIDE 提交 1999-02-08 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
1–327（327 aa）
|
未记录 | SAM S-ADENOSYLMETHIONINE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 6；2.2M AMMONIUM SULFATE 50 MMSODIUM CITRATE PH 6.0
|
分辨率 2.61 Å |
| 2HR1 Ternary structure of WT M.HhaI C5-Cytosine DNA methyltransferase with unmodified DNA and AdoHcy 提交 2006-07-19 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–327（327 aa）
|
未记录 | SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6；290 K；Ammonium Sulfate 15%, Sodium Citrate 100 mM, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K
|
分辨率 1.96 Å R-free 0.269 |
| 2I9K Engineered Extrahelical Base Destabilization Enhances Sequence Discrimination of DNA Methyltransferase M.HhaI 提交 2006-09-05 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–327（327 aa）
|
突变：F124A | SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 6；290 K；20% Ammonium Sulfate, 100mM Sodium Citrate buffer, pH 6.0, hanging drop, temperature 290K
|
分辨率 2.65 Å R-free 0.262 |
| 2UYC HhaI DNA methyltransferase R163N mutant complex with 13mer GCGC-GMGC oligonucleotide and SAH 提交 2007-04-03 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–327（327 aa）
|
突变：YES | SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 SO4 SULFATE ION × 6 GOL GLYCEROL × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 5.6；50 MM NA CITRATE PH 5.6, 1.8 M AMMONIUM SULFATE, 5% GLUCOSE
|
分辨率 2.00 Å R-free 0.227 |
| 2UYH HhaI DNA methyltransferase S87Q-Q237S mutant complex with 13mer GCGC- GMGC oligonucleotide and SAH 提交 2007-04-06 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–327（327 aa）
|
突变：YES | SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 SO4 SULFATE ION × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 5.6；50 MM NA CITRATE PH 5.6, 1.6 M AMMONIUM SULFATE, 5% GLUCOSE
|
分辨率 2.63 Å R-free 0.258 |
| 2UZ4 HhaI DNA methyltransferase R165N mutant complex with 13mer GCGC-GMGC oligonucleotide and SAH 提交 2007-04-25 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–327（327 aa）
|
突变：YES | SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 SO4 SULFATE ION × 4 GOL GLYCEROL × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 5.6；50 MM NA CITRATE PH 5.6, 1.8 M AMMONIUM SULFATE
|
分辨率 2.10 Å R-free 0.235 |
| 2Z6A S-Adenosyl-L-methionine-Dependent Methyl Transfer: Observable Precatalytic Intermediates during DNA Cytosine Methylation 提交 2007-07-25 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–327（327 aa）
|
突变：C81A | SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.5；290 K；2.0M Ammonium Sulfate, 50mM Sodium Citrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K
|
分辨率 2.88 Å R-free 0.280 |
| 2Z6Q Ternary structure of Arg165Ala M.HhaI C5-Cytosine DNA methyltransferase with unmodified DNA and AdoHcy 提交 2007-08-08 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：tetrameric（4） 与全部聚合物一致 |
链 A
1–327（327 aa）
|
突变：R165A | DCZ 2'-DEOXYCYTIDINE × 1 SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6；290 K；100 mM sodium citrate (pH 6.0), ammonium sulfate 1.2M, VAPOR DIFFUSION, HANGING DROP, temperature 290K
|
分辨率 2.79 Å R-free 0.269 |
| 2Z6U Ternary structure of the Glu119Ala M.HhaI, C5-Cytosine DNA methyltransferase, with unmodified DNA and AdoHcy 提交 2007-08-09 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–327（327 aa）
|
突变：E119A | SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6；290 K；100mM sodium citrate buffer, 45%-55% 3.5M ammonium sulfate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K
|
分辨率 2.72 Å R-free 0.267 |
| 2ZCJ Ternary structure of the Glu119Gln M.HhaI, C5-Cytosine DNA methyltransferase, with unmodified DNA and AdoHcy 提交 2007-11-09 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–327（327 aa）
|
突变：E119Q | SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6；290 K；100 mM Sodium Citrate, 1.6-2.1 Ammonium Sulfate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K
|
分辨率 2.75 Å R-free 0.266 |
| 3EEO M. HhaI co-crystallized with synthetic dsDNA containing a propane diol in place of the deoxycytidine residue targeted for methylation. 提交 2008-09-05 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–327（327 aa）
|
未记录 | SAM S-ADENOSYLMETHIONINE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；293 K；VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 1.94 Å R-free 0.275 |
| 3MHT TERNARY STRUCTURE OF HHAI METHYLTRANSFERASE WITH UNMODIFIED DNA AND ADOHCY 提交 1996-07-24 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–327（327 aa）
|
未记录 | SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 | X-RAY DIFFRACTION | mmCIF 未提供已读取条件 | 分辨率 2.70 Å |
| 4MHT TERNARY STRUCTURE OF HHAI METHYLTRANSFERASE WITH NATIVE DNA AND ADOHCY 提交 1996-07-24 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–327（327 aa）
|
未记录 | SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 | X-RAY DIFFRACTION | mmCIF 未提供已读取条件 | 分辨率 2.70 Å |
| 5CIY Structural basis of the recognition of H3K36me3 by DNMT3B PWWP domain 提交 2015-07-13 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–327（327 aa）
|
未记录 | SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 SO4 SULFATE ION × 8 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 5.6；298 K；AMMONIUM SULFATE 1.8 M, CITRATE 50mM
|
分辨率 1.59 Å R-free 0.188 |
| 5LOD Crystal structure of HhaI DNA methyltransferase in APO form 提交 2016-08-09 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
1–327（327 aa）
链 B
1–327（327 aa）
|
未记录 | SO4 SULFATE ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6；298 K；AMMONIUM SULFATE 50 mM, CITRATE 100mM, 32% (w/v) PEG-4000
|
分辨率 1.90 Å R-free 0.219 |
| 5MHT TERNARY STRUCTURE OF HHAI METHYLTRANSFERASE WITH HEMIMETHYLATED DNA AND ADOHCY 提交 1996-10-22 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–327（327 aa）
|
未记录 | SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 6.5；10% PEG 8000, 50 MM CA-ACETATE, 50 MM SODIUM CACODYLATE PH 6.5
|
分辨率 2.70 Å |
| 6MHT TERNARY STRUCTURE OF HHAI METHYLTRANSFERASE WITH ADOHCY AND DNA CONTAINING 4'-THIO-2'DEOXYCYTIDINE AT THE TARGET 提交 1998-08-05 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–327（327 aa）
|
未记录 | SAM S-ADENOSYLMETHIONINE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 5.6；293 K；1.4-1.8 M AMMONIUM SULFATE 50 MM CITRATE PH 5.6, temperature 293.0K
|
分辨率 2.05 Å R-free 0.231 |
| 7MHT CYTOSINE-SPECIFIC METHYLTRANSFERASE HHAI/DNA COMPLEX 提交 1998-08-05 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–327（327 aa）
|
未记录 | SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 5.6；1.4-1.8 M AMMONIUM SULFATE 50 MM CITRATE PH 5.6
|
分辨率 2.87 Å |
| 8MHT CYTOSINE-SPECIFIC METHYLTRANSFERASE HHAI/DNA COMPLEX 提交 1998-08-06 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–327（327 aa）
|
未记录 | SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 5.6；1.4-1.8 M AMMONIUM SULFATE 50 MM CITRATE PH 5.6
|
分辨率 2.76 Å |
| 9MHT CYTOSINE-SPECIFIC METHYLTRANSFERASE HHAI/DNA COMPLEX 提交 1998-08-07 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
1–327（327 aa）
|
未记录 | SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 5.6；10% (W/V) POLYETHYLENE GLYCOL 8000 50 MM CA-ACETATE 50 MM SODIUM CACODYLATE PH 5.6
|
分辨率 2.39 Å |