Current Protein Identity:P06028
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 8CRT Local refinement of Rh trimer, glycophorin B and Band3-III transmembrane region, class 1a of erythrocyte ankyrin-1 complex Deposited 2022-05-11 | Assembly 1 Other combination Heteromer;Protein × 8 PDB declaration: octameric(8) Consistent with protein count |
Chain P
1–91(91 aa)
|
Not recorded | CLR CHOLESTEROL × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4;Final gel filtration buffer contained 0.05% w/v digitonin, 130 mM KCl, 20 mM HEPES, pH 7.4, 1 mM ATP, 1 mM MgCl2, 1 mM PMSF. Peak fractions were concentrated to 8 mg/mL, and 0.01% w/v glycyrrhizic acid was added immediately prior to vitrification.
cryo-EM vitrification conditions
Cryogen ETHANE;4-6 seconds, wait time 30 seconds.
|
Resolution 3.00 Å |
| 8CS9 Composite reconstruction of Class 1 of the erythrocyte ankyrin-1 complex Deposited 2022-05-12 | Assembly 1 Other combination Heteromer;Protein × 18 PDB declaration: octadecameric(18) Consistent with protein count |
Chain P
1–91(91 aa)
|
Not recorded | CLR CHOLESTEROL × 8 AJP Digitonin × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 6 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4;Final gel filtration buffer contained 0.05% w/v digitonin, 130 mM KCl, 20 mM HEPES, pH 7.4, 1 mM ATP, 1 mM MgCl2, 1 mM PMSF. Peak fractions were concentrated to 8 mg/mL, and 0.01% w/v glycyrrhizic acid was added immediately prior to vitrification.
cryo-EM vitrification conditions
Cryogen ETHANE;4-6 seconds, wait time 30 seconds
|
Resolution 2.74 Å |
| 8CSL Sub-tomogram averaging of erythrocyte ankyrin-1 complex Deposited 2022-05-12 | Assembly 1 Protein heterocomplex Heteromer;Protein × 19 PDB declaration: nonadecameric(19) Consistent with protein count |
Chain P
1–91(91 aa)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4;130 mM KCl, 10 mM HEPES, pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 25.00 Å |