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Protein Structures and Oligomeric States

Enter a protein name, UniProt accession or PDB ID. Name searches ask you to confirm the protein identity before related structures are queried.

Current Protein Identity：P06280 New Search
31PDB entries
31Biological assembly
62chain/assembly records
Main Difference Dimensions in This Set
Different mutation/modification Different assembly state Different ligand/ion Different experimental conditions Different structure-quality metrics

Difference tags compare only the current result set; every original PDB and assembly record remains separate.

Related-Structure Differences

Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.

PDB Entry Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Experimental Method Experimental Conditions Structure Quality
1R46 Structure of human alpha-galactosidase Deposited 2003-10-03 Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 32–429（398 aa）
Chain B 32–429（398 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 EDO 1,2-ETHANEDIOL × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；30% PEG 4000, 0.1M TRIS HCL (PH 8.0), 0.2M AMMONIUM SULFATE, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 3.25 Å R-free 0.301
1R47 Structure of human alpha-galactosidase Deposited 2003-10-03 Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 32–429（398 aa）
Chain B 32–429（398 aa）
Not recorded GAL beta-D-galactopyranose × 2 EDO 1,2-ETHANEDIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；30% PEG 4000, 0.1M TRIS HCl, 0.2M AMMONIUM SULFATE, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 3.45 Å R-free 0.321
3GXN Crystal structure of apo alpha-galactosidase A at pH 4.5 Deposited 2009-04-02 Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 32–429（398 aa）
Chain B 32–429（398 aa）
Not recorded SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；vapor diffusion
Resolution 3.01 Å R-free 0.301
3GXP Crystal structure of acid-alpha-galactosidase A complexed with galactose at pH 4.5 Deposited 2009-04-02 Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 32–429（398 aa）
Chain B 32–429（398 aa）
Not recorded SO4 SULFATE ION × 4 GLA alpha-D-galactopyranose × 2 TAM TRIS(HYDROXYETHYL)AMINOMETHANE × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 MAN alpha-D-mannopyranose × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；vapor diffusion
Resolution 2.20 Å R-free 0.265
3GXT Crystal structure of alpha-galactosidase A at pH 4.5 complexed with 1-deoxygalactonijirimycin Deposited 2009-04-02 Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 32–429（398 aa）
Chain B 32–429（398 aa）
Not recorded SO4 SULFATE ION × 5 NOJ 1-DEOXYNOJIRIMYCIN × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；vapor diffusion
Resolution 2.70 Å R-free 0.306
3HG2 Human alpha-galactosidase catalytic mechanism 1. Empty active site Deposited 2009-05-13 Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 32–429（398 aa）
Chain B 32–429（398 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 SO4 SULFATE ION × 3 ACY ACETIC ACID × 3 GAL beta-D-galactopyranose × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.6；293 K；PEG4000, Ammonium Sulfate, Sodium Acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.30 Å R-free 0.202
3HG3 Human alpha-galactosidase catalytic mechanism 2. Substrate bound Deposited 2009-05-13 Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 32–429（398 aa）
Chain B 32–429（398 aa）
Mutation：D170A Mutation：D170A NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 2PE NONAETHYLENE GLYCOL × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；PEG8000, Magnesium acetate, Sodium Cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.90 Å R-free 0.197
3HG4 Human alpha-galactosidase catalytic mechanism 3. Covalent intermediate Deposited 2009-05-13 Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 32–429（398 aa）
Chain B 32–429（398 aa）
Not recorded 7JZ 2-deoxy-2,2-difluoro-beta-D-lyxo-hexopyranose × 2 SO4 SULFATE ION × 3 ACY ACETIC ACID × 4 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 GAL beta-D-galactopyranose × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.6；293 K；PEG4000, Ammonium Sulfate, Sodium Acetate, pH 4.6, vapor diffusion, hanging drop, temperature 293K
Resolution 2.30 Å R-free 0.221
3HG5 Human alpha-galactosidase catalytic mechanism 4. Product bound Deposited 2009-05-13 Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 32–429（398 aa）
Chain B 32–429（398 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 GLA alpha-D-galactopyranose × 2 SO4 SULFATE ION × 4 ACY ACETIC ACID × 4 GAL beta-D-galactopyranose × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.6；293 K；PEG4000, Ammonium Sulfate, Sodium Acetate, pH 4.6, vapor diffusion, hanging drop, temperature 293K
Resolution 2.30 Å R-free 0.227
3LX9 Interconversion of Human Lysosomal Enzyme Specificities Deposited 2010-02-25 Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 32–429（398 aa） Fragment：UNP residues 32-429
Chain B 32–429（398 aa） Fragment：UNP residues 32-429
Mutation：E203S,L206A Mutation：E203S,L206A NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；PEG8000, Sodium Cacodylate, Magnesium Acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.04 Å R-free 0.218
3LXA Interconversion of Human Lysosomal Enzyme Specificities Deposited 2010-02-25 Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 32–429（398 aa） Fragment：UNP residues 32-429
Chain B 32–429（398 aa） Fragment：UNP residues 32-429
Mutation：E203S L206A Mutation：E203S L206A NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 GLA alpha-D-galactopyranose × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；PEG8000, Sodium Cacodylate, Magnesium Acetate, pH 6.5, vapor diffusion, hanging drop, temperature 293K
Resolution 3.04 Å R-free 0.244
3LXB Interconversion of Human Lysosomal Enzyme Specificities Deposited 2010-02-25 Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 32–429（398 aa） Fragment：UNP residues 32-429
Chain B 32–429（398 aa） Fragment：UNP residues 32-429
Mutation：E203S,L206A Mutation：E203S,L206A NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 GOL GLYCEROL × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；PEG8000, Sodium Cacodylate, Magnesium Acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.85 Å R-free 0.264
3LXC Interconversion of Human Lysosomal Enzyme Specificities Deposited 2010-02-25 Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 32–429（398 aa） Fragment：UNP residues 32-429
Chain B 32–429（398 aa） Fragment：UNP residues 32-429
Mutation：E203S,L206A Mutation：E203S,L206A NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 GOL GLYCEROL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；PEG8000, Sodium Cacodylate, Magnesium Acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.35 Å R-free 0.237
3S5Y Pharmacological Chaperoning in Human alpha-Galactosidase Deposited 2011-05-23 Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 32–429（398 aa） Fragment：UNP Residues 32-429
Chain B 32–429（398 aa） Fragment：UNP Residues 32-429
Not recorded DGJ (2R,3S,4R,5S)-2-(hydroxymethyl)piperidine-3,4,5-triol × 2 2PE NONAETHYLENE GLYCOL × 5 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 EDO 1,2-ETHANEDIOL × 1 ACY ACETIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.1；293 K；8-15% PEG 8000, 0.1 M Sodium Cacodylate, 0.02 M Magnesium Chloride, pH 5.1, vapor diffusion, hanging drop, temperature 293K, VAPOR DIFFUSION, HANGING DROP
Resolution 2.10 Å R-free 0.230
3S5Z Pharmacological Chaperoning in Human alpha-Galactosidase Deposited 2011-05-23 Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 32–429（398 aa）
Chain B 32–429（398 aa）
Not recorded GLA alpha-D-galactopyranose × 2 GAL beta-D-galactopyranose × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.1；293 K；PEG 8000, Sodium Cacodylate, Magnesium Chloride, pH 5.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.00 Å R-free 0.234
3TV8 Pharmacological Chaperoning in Human alpha-Galactosidase Deposited 2011-09-19 Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 32–429（398 aa） Fragment：UNP Residues 32-429
Chain B 32–429（398 aa） Fragment：UNP Residues 32-429
Mutation：D170A Mutation：D170A NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 SO4 SULFATE ION × 3 2PE NONAETHYLENE GLYCOL × 3 DGJ (2R,3S,4R,5S)-2-(hydroxymethyl)piperidine-3,4,5-triol × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.6；293 K；20% PEG 4000, 200mM Ammonium Sulfate, 100mM Sodium Acetate, pH 4.6, vapor diffusion, hanging drop, temperature 293K
Resolution 2.64 Å R-free 0.239
4NXS Crystal structure of human alpha-galactosidase A in complex with 1-deoxygalactonojirimycin-pFPhT Deposited 2013-12-09 Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 32–429（398 aa）
Chain B 32–429（398 aa）
Not recorded SO4 SULFATE ION × 5 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 2OZ (2R,3S,4R,5S)-N-(4-fluorophenyl)-3,4,5-trihydroxy-2-(hydroxymethyl)piperidine-1-carbothioamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.5；298 K；0.1 M acetate pH 4.5, 0.215 M ammonium sulfate, and 24% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.55 Å R-free 0.232
6IBK Crystal structure of human alpha-galactosidase A in complex with alpha-galactose configured cyclosulfamidate ME763 Deposited 2018-11-30 Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 32–429（398 aa）
Chain B 32–429（398 aa）
Not recorded ACT ACETATE ION × 3 SO4 SULFATE ION × 11 EDO 1,2-ETHANEDIOL × 12 YTW (3~{a}~{R},4~{S},5~{S},6~{S},7~{R},7~{a}~{S})-7-(hydroxymethyl)-2,2-bis(oxidanylidene)-3~{a},4,5,6,7,7~{a}-hexahydro-3~{H}-benzo[d][1,2,3]oxathiazole-4,5,6-triol × 2 PEG DI(HYDROXYETHYL)ETHER × 5 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 4000 (50%), sodium acetate (pH 4.6) and lithium sulfate,
Resolution 1.99 Å R-free 0.244
6IBM Crystal structure of human alpha-galactosidase A in complex with alpha-galactose configured cyclosulfate ME776 Deposited 2018-11-30 Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 32–429（398 aa）
Chain B 32–429（398 aa）
Not recorded PEG DI(HYDROXYETHYL)ETHER × 2 ACT ACETATE ION × 4 SO4 SULFATE ION × 6 HF8 [(1~{S},2~{R},3~{S},4~{S},5~{R},6~{S})-2-(hydroxymethyl)-3,4,5,6-tetrakis(oxidanyl)cyclohexyl] hydrogen sulfate × 2 EDO 1,2-ETHANEDIOL × 18 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 4000 (50%), sodium acetate (pH 4.6) and lithium sulfate
Resolution 2.07 Å R-free 0.242
6IBR Crystal structure of human alpha-galactosidase A in complex with alpha-galactose configured cyclophellitol epoxide LWA481 Deposited 2018-11-30 Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 32–429（398 aa）
Chain B 32–429（398 aa）
Not recorded SO4 SULFATE ION × 7 EDO 1,2-ETHANEDIOL × 9 PEG DI(HYDROXYETHYL)ETHER × 4 ACT ACETATE ION × 6 HBE (2~{R},3~{S},4~{S},5~{R},6~{S})-5-(hydroxymethyl)-7-oxabicyclo[4.1.0]heptane-2,3,4-triol × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 4000 (50%), sodium acetate (pH 4.6) and lithium sulfate
Resolution 2.02 Å R-free 0.256
6IBT Crystal structure of human alpha-galactosidase A in complex with alpha-galactose configured cyclophellitol aziridine ME737 Deposited 2018-11-30 Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 32–429（398 aa）
Chain B 32–429（398 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 EDO 1,2-ETHANEDIOL × 7 PEG DI(HYDROXYETHYL)ETHER × 3 H9T (1~{S},2~{S},3~{S},4~{S},5~{R},6~{S})-5-(hydroxymethyl)-7-azabicyclo[4.1.0]heptane-2,3,4-triol × 2 SO4 SULFATE ION × 5 ACT ACETATE ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 4000 (50%), sodium acetate (pH 4.6) and lithium sulfate
Resolution 2.04 Å R-free 0.260
8K7D Crystal structure of human lysosomal alpha-galactosidase A in complex with (2R,3R,4S,5R)-2-(aminomethyl)-5-(hydroxymethyl)pyrrolidine-3,4-diol Deposited 2023-07-26 Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 32–429（398 aa）
Chain B 32–429（398 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 VLN (2~{R},3~{R},4~{S},5~{R})-2-(aminomethyl)-5-(hydroxymethyl)pyrrolidine-3,4-diol × 2 SO4 SULFATE ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.5；293 K；100 mM NaOAc pH 4.5, 25% PEG4000 and 200 mM ammonium sulfate
Resolution 2.61 Å R-free 0.209
8K7E Crystal structure of human lysosomal alpha-galactosidase A in complex with (2R,3R,4S,5R)-2-(aminomethyl)-5-(hydroxymethyl)pyrrolidine-3,4-diol Deposited 2023-07-26 Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 32–429（398 aa）
Chain B 32–429（398 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 VLN (2~{R},3~{R},4~{S},5~{R})-2-(aminomethyl)-5-(hydroxymethyl)pyrrolidine-3,4-diol × 2 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.2；293 K；100 mM Tris-HCl buffer 7.2, 25% PEG4000 and 200 mM ammonium sulfate
Resolution 2.20 Å R-free 0.192
8K7F Crystal structure of human lysosomal alpha-galactosidase A in complex with (2R,3S,4R,5R)-2,5-bis(hydroxymethyl)pyrrolidine-3,4-diol Deposited 2023-07-26 Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 32–429（398 aa）
Chain B 32–429（398 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 VMF (2~{R},3~{R},4~{S},5~{R})-2,5-bis(hydroxymethyl)pyrrolidine-3,4-diol × 2 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.5；293 K；100 mM NaOAc pH 4.5, 25% PEG4000 and 200 mM ammonium sulfate
Resolution 1.98 Å R-free 0.191
8K7G Crystal structure of human lysosomal alpha-galactosidase A in complex with (2R,3S,4R,5R)-2,5-bis(hydroxymethyl)pyrrolidine-3,4-diol Deposited 2023-07-26 Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 32–429（398 aa）
Chain B 32–429（398 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 VMF (2~{R},3~{R},4~{S},5~{R})-2,5-bis(hydroxymethyl)pyrrolidine-3,4-diol × 2 SO4 SULFATE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.2；293 K；100 mM Tris-HCl buffer 7.2, 25% PEG4000 and 200 mM ammonium sulfate
Resolution 2.32 Å R-free 0.217
8K7H Crystal structure of human lysosomal alpha-galactosidase A in complex with (2R,3S,4R)-2-(hydroxymethyl)-1-methylpyrrolidine-3,4-diol Deposited 2023-07-26 Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 32–429（398 aa）
Chain B 32–429（398 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 VMO (2~{R},3~{S},4~{R})-2-(hydroxymethyl)-1-methyl-pyrrolidine-3,4-diol × 2 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.2；293 K；100 mM Tris-HCl buffer 7.2, 25% PEG4000 and 200 mM ammonium sulfate
Resolution 2.28 Å R-free 0.210
8K7I Crystal structure of human lysosomal alpha-galactosidase A in complex with (2R,3S,4R,5R)-2-(hydroxymethyl)-5-((methylamino)methyl)pyrrolidine-3,4-diol Deposited 2023-07-26 Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 32–429（398 aa）
Chain B 32–429（398 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 VN0 (2~{R},3~{S},4~{R},5~{R})-2-(hydroxymethyl)-5-(methylaminomethyl)pyrrolidine-3,4-diol × 2 SO4 SULFATE ION × 5 P6G HEXAETHYLENE GLYCOL × 1 GOL GLYCEROL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.2；293 K；100 mM Tris-HCl buffer 7.2, 25% PEG4000 and 200 mM ammonium sulfate
Resolution 2.12 Å R-free 0.195
8K7J Crystal structure of human lysosomal alpha-galactosidase A in complex with (2R,3R,4S,5R)-2-((dimethylamino)methyl)-5-(hydroxymethyl)pyrrolidine-3,4-diol Deposited 2023-07-26 Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 32–429（398 aa）
Chain B 32–429（398 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 VNB (2~{R},3~{R},4~{S},5~{R})-2-[(dimethylamino)methyl]-5-(hydroxymethyl)pyrrolidine-3,4-diol × 2 SO4 SULFATE ION × 5 P6G HEXAETHYLENE GLYCOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.2；293 K；100 mM Tris-HCl buffer 7.2, 25% PEG4000 and 200 mM ammonium sulfate
Resolution 2.01 Å R-free 0.191
8K7K Crystal structure of human lysosomal alpha-galactosidase A in complex with (2R,3S,4R,5R)-2,5-bis(hydroxymethyl)-1-methylpyrrolidine-3,4-diol Deposited 2023-07-26 Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 32–429（398 aa）
Chain B 32–429（398 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 VNQ (2~{R},3~{S},4~{R},5~{R})-2,5-bis(hydroxymethyl)-1-methyl-pyrrolidine-3,4-diol × 2 SO4 SULFATE ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.2；293 K；100 mM Tris-HCl buffer 7.2, 25% PEG4000 and 200 mM ammonium sulfate
Resolution 2.00 Å R-free 0.186
8K7L Crystal structure of human lysosomal alpha-galactosidase A in complex with (2R,3R,4S,5R)-2-(aminomethyl)-5-(hydroxymethyl)-1-methylpyrrolidine-3,4-diol Deposited 2023-07-26 Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 32–429（398 aa）
Chain B 32–429（398 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 VO0 (2~{R},3~{R},4~{S},5~{R})-2-(aminomethyl)-5-(hydroxymethyl)-1-methyl-pyrrolidine-3,4-diol × 2 SO4 SULFATE ION × 5 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.2；293 K；100 mM Tris-HCl buffer 7.2, 25% PEG4000 and 200 mM ammonium sulfate
Resolution 2.00 Å R-free 0.186
9AVS Human alpha-galactosidase A in complex with saposin B Deposited 2024-03-04 Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count
Chain A 32–429（398 aa）
Chain B 32–429（398 aa）
Mutation：D170A Mutation：D170A SO4 SULFATE ION × 8 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；500 nL of protein (0.1 mM GLA plus 0.22 mM SapB) was mixed with 500 nL of reservoir solution (0.1 M Tris pH 8.0, 1 M Ammonium sulfate) and equilibrated against 70 uL reservoirs at 20 C.
Resolution 3.53 Å R-free 0.265

This page presents structural evidence recorded in PDB/mmCIF.“No recorded non-water small molecule” does not prove that the experimental sample was ligand-free; experimental conditions belong to the PDB experiment, while ligands and copy counts are shown for each biological assembly.