当前蛋白身份：P06971
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差异标签只比较当前检索结果；所有PDB和assembly原始记录仍分别保留。
相关结构差异明细
一行代表一个 PDB 条目中的一个 biological assembly；同一蛋白的多个单体会分别列出。
| PDB 条目 | Assembly / 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与非聚合物 | 实验方法 | 实验环境 | 结构质量 |
|---|---|---|---|---|---|---|---|
| 1BY3 FHUA FROM E. COLI 提交 1998-10-22 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
34–747（714 aa）
|
未记录 | OES N-OCTYL-2-HYDROXYETHYL SULFOXIDE × 7 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 6.2；PROTEIN WAS CRYSTALLIZED FROM 33% PEG2000, 0.45 M NACL, 0.15 M NAPI (PH 6.2)
AND 0.5% N-OCTYL-2-HYDROXYETHYLSULFOXIDE
|
分辨率 2.74 Å R-free 0.244 |
| 1FCP FERRIC HYDROXAMATE UPTAKE RECEPTOR (FHUA) FROM E.COLI IN COMPLEX WITH BOUND FERRICHROME-IRON 提交 1998-10-14 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
52–747（696 aa）
|
非标准单体：是（mmCIF未提供具体位点） | NI NICKEL (II) ION × 2 LIL 2-TRIDECANOYLOXY-PENTADECANOIC ACID × 2 AAE ACETOACETIC ACID × 1 LIM 3-OXO-PENTADECANOIC ACID × 1 EA2 AMINOETHANOLPYROPHOSPHATE × 1 FCI FERRICROCIN-IRON × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 6.4；100MM NA-CACODYLATE, PH 6.4, 12.5% PEG2000 MME, 20% GLYCEROL, 3% PEG200
|
分辨率 2.70 Å R-free 0.281 |
| 1FI1 FhuA in complex with lipopolysaccharide and rifamycin CGP4832 提交 2000-08-03 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
52–747（696 aa）
|
突变：HEXAHISTIDINE TAG PLUS FIVE LINKER RESIDUES HAVE BEEN GENETICALLY INSERTED AFTER RESIDUE 405 OF THE MATURE SEQUENCE | FTT 3-HYDROXY-TETRADECANOIC ACID × 6 PO4 PHOSPHATE ION × 2 NI NICKEL (II) ION × 1 NA SODIUM ION × 1 MG MAGNESIUM ION × 1 DPO DIPHOSPHATE × 2 RIF RIFAMYCIN CGP 4832 × 1 DDQ DECYLAMINE-N,N-DIMETHYL-N-OXIDE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.4；291 K；100 mM sodium cacodylate, 12% PEG 2,000 MME, 3% PEG 200, 1% cis-inositol, 20% glycerol, 1 mM rifamycin CGP 4832, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
分辨率 2.90 Å R-free 0.275 |
| 1QFF E. COLI FERRIC HYDROXAMATE UPTAKE RECEPTOR (FHUA) IN COMPLEX WITH BOUND FERRICHROME-IRON 提交 1999-04-10 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
34–747（714 aa）
|
突变：SER-SER-HIS-HIS-HIS-HIS-HIS-HIS-GLY-SER-SER AFFINITY TAG 非标准单体：是（mmCIF未提供具体位点） | PO4 PHOSPHATE ION × 2 FTT 3-HYDROXY-TETRADECANOIC ACID × 4 DAO LAURIC ACID × 1 MYR MYRISTIC ACID × 1 DPO DIPHOSPHATE × 1 EAP 2-AMINO-VINYL-PHOSPHATE × 1 FCI FERRICROCIN-IRON × 1 DDQ DECYLAMINE-N,N-DIMETHYL-N-OXIDE × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 6.4；pH 6.4
|
分辨率 2.70 Å R-free 0.276 |
| 1QFG E. COLI FERRIC HYDROXAMATE RECEPTOR (FHUA) 提交 1999-04-10 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
34–747（714 aa）
|
突变：YES | NI NICKEL (II) ION × 1 PO4 PHOSPHATE ION × 2 FTT 3-HYDROXY-TETRADECANOIC ACID × 4 DAO LAURIC ACID × 1 MYR MYRISTIC ACID × 1 DDQ DECYLAMINE-N,N-DIMETHYL-N-OXIDE × 3 DPO DIPHOSPHATE × 2 GOL GLYCEROL × 8 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.4；290 K；100MM SODIUM CACODYLATE (PH 6.4), 12.5% PEG 2000 MONOMETHYLETHER, 20% GLYCEROL,
1% CIS-INOSITOL, 3% PEG 200, VAPOR DIFFUSION, HANGING DROP, temperature 290K
|
分辨率 2.50 Å R-free 0.271 |
| 1QJQ FERRIC HYDROXAMATE RECEPTOR FROM ESCHERICHIA COLI (FHUA) 提交 1999-06-29 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
34–438（405 aa）
链 A
439–747（309 aa）
|
突变：YES 突变：YES | FTT 3-HYDROXY-TETRADECANOIC ACID × 6 DPO DIPHOSPHATE × 2 PO4 PHOSPHATE ION × 2 NI NICKEL (II) ION × 1 PFC PHENYLFERRICROCIN-IRON × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 6.4；100MM SODIUM CACODYLATE (PH 6.4), 14.5% PEG 2000 MONOMETHYLETHER, 20% GLYCEROL, 3% PEG 200, 1 MM PHENYLFERRICROCIN-IRON
|
分辨率 2.95 Å R-free 0.278 |
| 1QKC ESCHERICHIA COLI FERRIC HYDROXAMATE UPTAKE RECEPTOR (FHUA) IN COMPLEX DELTA TWO-ALBOMYCIN 提交 1999-07-18 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
34–438（405 aa）
链 A
439–747（309 aa）
|
突变：YES 突变：YES | FTT 3-HYDROXY-TETRADECANOIC ACID × 6 DPO DIPHOSPHATE × 2 PO4 PHOSPHATE ION × 2 NI NICKEL (II) ION × 1 ALB DELTA-2-ALBOMYCIN A1 × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 6.4；100 MM SODIUM CACODYLATE (PH 6.4), 14.0% POLYETHYLGLYCOL 2000 MONOMETHYLETHER, 20% GLYCEROL, 3% POLYETHYLGLYCOL 200, 1% CIS-INOSITOL, 0.3 MM DELTA TWO ALBOMYCIN
|
分辨率 3.10 Å R-free 0.283 |
| 2FCP FERRIC HYDROXAMATE UPTAKE RECEPTOR (FHUA) FROM E.COLI 提交 1998-10-15 | Assembly 1 其他组合 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
34–747（714 aa）
|
未记录 | NI NICKEL (II) ION × 2 LIL 2-TRIDECANOYLOXY-PENTADECANOIC ACID × 2 AAE ACETOACETIC ACID × 1 LIM 3-OXO-PENTADECANOIC ACID × 1 EA2 AMINOETHANOLPYROPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 6.4；100MM NA-CACODYLATE, PH 6.4, 12.5% PEG2000 MME, 20% GLYCEROL, 1% CIS-INOSITOL, 3% PEG200
|
分辨率 2.50 Å R-free 0.283 |
| 2GRX Crystal structure of TonB in complex with FhuA, E. coli outer membrane receptor for ferrichrome 提交 2006-04-25 | Assembly 1 其他组合 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
34–758（725 aa）
|
未记录 | PO4 PHOSPHATE ION × 1 FTT 3-HYDROXY-TETRADECANOIC ACID × 4 DPO DIPHOSPHATE × 1 DAO LAURIC ACID × 1 EAP 2-AMINO-VINYL-PHOSPHATE × 1 FCI FERRICROCIN-IRON × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6；291 K；8% polyethylene glycol 4000, 50 mM MES (pH 6.0), 75 mM NaCl, 5% glycerol, 0.7% C8E4, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
分辨率 3.30 Å R-free 0.329 |
| 2GRX Crystal structure of TonB in complex with FhuA, E. coli outer membrane receptor for ferrichrome 提交 2006-04-25 | Assembly 2 其他组合 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 B
34–758（725 aa）
|
未记录 | PO4 PHOSPHATE ION × 1 FTT 3-HYDROXY-TETRADECANOIC ACID × 4 DPO DIPHOSPHATE × 1 DAO LAURIC ACID × 1 EAP 2-AMINO-VINYL-PHOSPHATE × 1 FCI FERRICROCIN-IRON × 1 MYR MYRISTIC ACID × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6；291 K；8% polyethylene glycol 4000, 50 mM MES (pH 6.0), 75 mM NaCl, 5% glycerol, 0.7% C8E4, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
分辨率 3.30 Å R-free 0.329 |
| 4CU4 FhuA from E. coli in complex with the lasso peptide microcin J25 (MccJ25) 提交 2014-03-17 | Assembly 1 其他组合 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
53–438（386 aa）
片段：RESIDUES 53-747
链 A
439–747（309 aa）
片段：RESIDUES 53-747
|
未记录 | FTT 3-HYDROXY-TETRADECANOIC ACID × 4 DAO LAURIC ACID × 1 MYR MYRISTIC ACID × 1 LDA LAURYL DIMETHYLAMINE-N-OXIDE × 20 3PH 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE × 1 DPO DIPHOSPHATE × 2 PO4 PHOSPHATE ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 5；20 MM TRIS PH 7.5, 120 MM LITHIUM SULFATE, 100 MM SODIUM CITRATE PH 5, 20 % PEG300
|
分辨率 2.30 Å R-free 0.255 |
| 8A60 Crystal structure of FhuA in complex with the superinfection exclusion lipoprotein Llp 提交 2022-06-16 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
34–747（714 aa）
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 4.6；293 K；12-16% PEG 4000
0.4 M sodium isothiocyanate
0.1 M sodium acetate
|
分辨率 3.37 Å R-free 0.307 |
| 8A8C T5 phage receptor-binding protein pb5 bound to ferrichrome transporter FhuA 提交 2022-06-22 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
34–747（714 aa）
|
未记录 | LU9 [(2R,3S,4R,5R,6R)-2-[[(2R,4R,5R,6R)-6-[(1R)-1,2-bis(oxidanyl)ethyl]-4-[(2R,4R,5R,6R)-6-[(1R)-1,2-bis(oxidanyl)ethyl]-2-carboxy-4,5-bis(oxidanyl)oxan-2-yl]oxy-2-carboxy-5-oxidanyl-oxan-2-yl]oxymethyl]-5-[[(3R)-3-dodecanoyloxytetradecanoyl]amino]-4-(3-nonanoyloxypropanoyloxy)-6-[[(2R,3S,4R,5R,6R)-3-oxidanyl-4-[(3S)-3-oxidanyltetradecanoyl]oxy-5-[[(3R)-3-oxidanyltridecanoyl]amino]-6-phosphonatooxy-oxan-2-yl]methoxy]oxan-3-yl] phosphate × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.10 Å |
| 8B14 T5 Receptor Binding Protein pb5 in complex with its E. coli receptor FhuA 提交 2022-09-09 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
34–747（714 aa）
|
突变：Presence of an Histag after P405, added residues : SHHHHHHGS | DDQ DECYLAMINE-N,N-DIMETHYL-N-OXIDE × 1 LU9 [(2R,3S,4R,5R,6R)-2-[[(2R,4R,5R,6R)-6-[(1R)-1,2-bis(oxidanyl)ethyl]-4-[(2R,4R,5R,6R)-6-[(1R)-1,2-bis(oxidanyl)ethyl]-2-carboxy-4,5-bis(oxidanyl)oxan-2-yl]oxy-2-carboxy-5-oxidanyl-oxan-2-yl]oxymethyl]-5-[[(3R)-3-dodecanoyloxytetradecanoyl]amino]-4-(3-nonanoyloxypropanoyloxy)-6-[[(2R,3S,4R,5R,6R)-3-oxidanyl-4-[(3S)-3-oxidanyltetradecanoyl]oxy-5-[[(3R)-3-oxidanyltridecanoyl]amino]-6-phosphonatooxy-oxan-2-yl]methoxy]oxan-3-yl] phosphate × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8.5
cryo-EM玻璃化条件
冷冻剂 ETHANE；3.5 microliters of the FhuA-pb5 complex were deposited on a freshly glow discharged (25 mA, 30 sec) Cu/Rh 400 mesh Quantifoil R 2/1 EM grids and flash-frozen in nitrogen-cooled liquid ethane using a ThermoFisher Mark IV Vitrobot device (100% humidity, 293.15K, 2s blotting time, blot force 1).
|
分辨率 2.60 Å |