当前蛋白身份：P06996
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差异标签只比较当前检索结果；所有PDB和assembly原始记录仍分别保留。
相关结构差异明细
一行代表一个 PDB 条目中的一个 biological assembly；同一蛋白的多个单体会分别列出。
| PDB 条目 | Assembly / 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与非聚合物 | 实验方法 | 实验环境 | 结构质量 |
|---|---|---|---|---|---|---|---|
| 2J1N osmoporin OmpC 提交 2006-08-15 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 3 PDB 声明：trimeric（3） 与蛋白数一致 |
链 A
22–367（346 aa）
链 B
22–367（346 aa）
链 C
22–367（346 aa）
|
未记录 | MG MAGNESIUM ION × 3 CL CHLORIDE ION × 2 D12 DODECANE × 11 | X-RAY DIFFRACTION |
X-ray结晶条件
25 MG/ML OMPC IN 25 MM TRIS-HCL (PH 8.0), 0.5 M NACL, 1.8 % (W/W) N-OCTYL-2- HYDROXYETHYLSULFOXIDE (C8HESO), 0.3 % (V/V) OCTYL-POLYOXYETHYLENE (OCTYL-POE) WERE EQUILIBRATED AGAINST A RESERVOIR SOLUTION CONTAINING 25 MM TRIS-HCL (PH 8.0), 0.5 M NACL AND 28% PEG2000.
|
分辨率 2.00 Å R-free 0.214 |
| 2J4U E.coli OmpC - camel Lactoferrin complex 提交 2006-09-06 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 P
22–367（346 aa）
片段：RESIDUES 22-367
链 Q
22–367（346 aa）
片段：RESIDUES 22-367
链 R
22–367（346 aa）
片段：RESIDUES 22-367
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION | mmCIF 未提供已读取条件 | 分辨率 2.99 Å R-free 0.282 |
| 2J4U E.coli OmpC - camel Lactoferrin complex 提交 2006-09-06 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 U
22–367（346 aa）
片段：RESIDUES 22-367
链 V
22–367（346 aa）
片段：RESIDUES 22-367
链 W
22–367（346 aa）
片段：RESIDUES 22-367
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION | mmCIF 未提供已读取条件 | 分辨率 2.99 Å R-free 0.282 |
| 2ZLE Cryo-EM structure of DegP12/OMP 提交 2008-04-09 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 13 PDB 声明：tridecameric（13） 与蛋白数一致 |
链 D
22–367（346 aa）
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM玻璃化条件
冷冻剂 ETHANE；Embedded in vitreous ice using C-flat holey carbon grids (CF-2/2-4C-100, Protochip) and a Vitrobot (FEI) at 20 temperature and 100% relative humidity
|
分辨率 28.00 Å |
| 3NB3 The host outer membrane proteins OmpA and OmpC are packed at specific sites in the Shigella phage Sf6 virion as structural components 提交 2010-06-02 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 240 PDB 声明：240-MERIC（240） 与蛋白数一致 |
链 D
22–367（346 aa）
片段：outer membrane protein C
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
10 mM Tris-HCl, pH 7.4, 10 mM MgCl2；pH 7.4；10 mM Tris-HCl, pH 7.4, 10 mM MgCl2
cryo-EM玻璃化条件
冷冻剂 ETHANE；Vitrification was carried out with a standard cryoEM method. The sample of 4 microlitres was applied to a holey EM grid, blotted for 3-4 seconds with a Whatman #2 filter paper, and plunged into ethane slush incubated in liquid nitrogen. The grid was stored in liquid nitrogen.
|
分辨率 19.00 Å |
| 3NB3 The host outer membrane proteins OmpA and OmpC are packed at specific sites in the Shigella phage Sf6 virion as structural components 提交 2010-06-02 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 D
22–367（346 aa）
片段：outer membrane protein C
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
10 mM Tris-HCl, pH 7.4, 10 mM MgCl2；pH 7.4；10 mM Tris-HCl, pH 7.4, 10 mM MgCl2
cryo-EM玻璃化条件
冷冻剂 ETHANE；Vitrification was carried out with a standard cryoEM method. The sample of 4 microlitres was applied to a holey EM grid, blotted for 3-4 seconds with a Whatman #2 filter paper, and plunged into ethane slush incubated in liquid nitrogen. The grid was stored in liquid nitrogen.
|
分辨率 19.00 Å |
| 3NB3 The host outer membrane proteins OmpA and OmpC are packed at specific sites in the Shigella phage Sf6 virion as structural components 提交 2010-06-02 | Assembly 3 蛋白异源复合物 异源复合物；蛋白 × 20 PDB 声明：eicosameric（20） 与蛋白数一致 |
链 D
22–367（346 aa）
片段：outer membrane protein C
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
10 mM Tris-HCl, pH 7.4, 10 mM MgCl2；pH 7.4；10 mM Tris-HCl, pH 7.4, 10 mM MgCl2
cryo-EM玻璃化条件
冷冻剂 ETHANE；Vitrification was carried out with a standard cryoEM method. The sample of 4 microlitres was applied to a holey EM grid, blotted for 3-4 seconds with a Whatman #2 filter paper, and plunged into ethane slush incubated in liquid nitrogen. The grid was stored in liquid nitrogen.
|
分辨率 19.00 Å |
| 3NB3 The host outer membrane proteins OmpA and OmpC are packed at specific sites in the Shigella phage Sf6 virion as structural components 提交 2010-06-02 | Assembly 4 蛋白异源复合物 异源复合物；蛋白 × 24 PDB 声明：24-meric（24） 与蛋白数一致 |
链 D
22–367（346 aa）
片段：outer membrane protein C
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
10 mM Tris-HCl, pH 7.4, 10 mM MgCl2；pH 7.4；10 mM Tris-HCl, pH 7.4, 10 mM MgCl2
cryo-EM玻璃化条件
冷冻剂 ETHANE；Vitrification was carried out with a standard cryoEM method. The sample of 4 microlitres was applied to a holey EM grid, blotted for 3-4 seconds with a Whatman #2 filter paper, and plunged into ethane slush incubated in liquid nitrogen. The grid was stored in liquid nitrogen.
|
分辨率 19.00 Å |
| 3NB3 The host outer membrane proteins OmpA and OmpC are packed at specific sites in the Shigella phage Sf6 virion as structural components 提交 2010-06-02 | Assembly 5 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 D
22–367（346 aa）
片段：outer membrane protein C
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
10 mM Tris-HCl, pH 7.4, 10 mM MgCl2；pH 7.4；10 mM Tris-HCl, pH 7.4, 10 mM MgCl2
cryo-EM玻璃化条件
冷冻剂 ETHANE；Vitrification was carried out with a standard cryoEM method. The sample of 4 microlitres was applied to a holey EM grid, blotted for 3-4 seconds with a Whatman #2 filter paper, and plunged into ethane slush incubated in liquid nitrogen. The grid was stored in liquid nitrogen.
|
分辨率 19.00 Å |
| 4A8D DegP dodecamer with bound OMP 提交 2011-11-20 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 13 PDB 声明：tridecameric（13） 与蛋白数一致 |
链 M
22–367（346 aa）
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
300MM NACL, 50MM HEPES- NAOH；pH 8；300MM NACL, 50MM HEPES- NAOH
cryo-EM玻璃化条件
冷冻剂 ETHANE；EMBEDDED IN VITREOUS ICE USING C-FLAT HOLEY CARBON GRIDS AND A VITROBOT AT 20C.
|
分辨率 28.00 Å |
| 8I8R Cryo-EM Structure of OmpC3-MlaA Complex in MSP2N2 Nanodiscs 提交 2023-02-05 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 A
22–367（346 aa）
链 B
22–367（346 aa）
链 C
22–367（346 aa）
|
未记录 | KDL (2~{R},4~{R},5~{R},6~{R})-6-[(1~{R})-1,2-bis(oxidanyl)ethyl]-2-[(2~{R},4~{R},5~{R},6~{R})-6-[(1~{R})-1,2-bis(oxidanyl)ethyl]-2-carboxy-2-[[(2~{R},3~{S},4~{R},5~{R},6~{R})-5-[[(3~{R})-3-dodecanoyloxytetradecanoyl]amino]-6-[[(2~{R},3~{S},4~{R},5~{R},6~{R})-3-oxidanyl-5-[[(3~{R})-3-oxidanyltetradecanoyl]amino]-4-[(3~{R})-3-oxidanyltetradecanoyl]oxy-6-phosphonooxy-oxan-2-yl]methoxy]-3-phosphonooxy-4-[(3~{R})-3-tetradecanoyloxytetradecanoyl]oxy-oxan-2-yl]methoxy]-5-oxidanyl-oxan-4-yl]oxy-4,5-bis(oxidanyl)oxane-2-carboxylic acid × 3 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8；Tris-buffered saline (TBS) buffer (20 mM Tris HCl pH 8.0, 150 mM NaCl)
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.93 Å |
| 8I8X Cryo-EM Structure of OmpC3-MlaA-MlaC Complex in MSP2N2 Nanodiscs 提交 2023-02-05 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 5 PDB 声明：pentameric（5） 与蛋白数一致 |
链 A
22–367（346 aa）
链 B
22–367（346 aa）
链 C
22–367（346 aa）
|
未记录 | KDL (2~{R},4~{R},5~{R},6~{R})-6-[(1~{R})-1,2-bis(oxidanyl)ethyl]-2-[(2~{R},4~{R},5~{R},6~{R})-6-[(1~{R})-1,2-bis(oxidanyl)ethyl]-2-carboxy-2-[[(2~{R},3~{S},4~{R},5~{R},6~{R})-5-[[(3~{R})-3-dodecanoyloxytetradecanoyl]amino]-6-[[(2~{R},3~{S},4~{R},5~{R},6~{R})-3-oxidanyl-5-[[(3~{R})-3-oxidanyltetradecanoyl]amino]-4-[(3~{R})-3-oxidanyltetradecanoyl]oxy-6-phosphonooxy-oxan-2-yl]methoxy]-3-phosphonooxy-4-[(3~{R})-3-tetradecanoyloxytetradecanoyl]oxy-oxan-2-yl]methoxy]-5-oxidanyl-oxan-4-yl]oxy-4,5-bis(oxidanyl)oxane-2-carboxylic acid × 3 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8；Tris-buffered saline (TBS) buffer (20 mM Tris HCl pH 8.0, 150 mM NaCl)
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.25 Å |