Current Protein Identity：P07276
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 2LOX NMR structure of the complex between the PH domain of the Tfb1 subunit from TFIIH and Rad2 Deposited 2012-01-27 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
642–690（49 aa）
Fragment：UNP residues 642-690
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 6.5；300 K；Pressure ambient
NMR sample composition
1 mM [U-100% 13C; U-100% 15N] Tfb1, 1.25 mM Rad2, 20 mM sodium phosphate, 1 mM EDTA, 1 mM DTT, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
1 mM [U-100% 13C; U-100% 15N] Tfb1, 1.25 mM Rad2, 20 mM sodium phosphate, 1 mM EDTA, 1 mM DTT, 100% D2O | 100% D2O
NMR sample composition
1 mM [U-100% 15N] Tfb1, 1.25 mM Rad2, 20 mM sodium phosphate, 1 mM EDTA, 1 mM DTT, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
1.25 mM Tfb1, 1 mM [U-100% 13C; U-100% 15N] Rad2, 20 mM sodium phosphate, 1 mM EDTA, 1 mM DTT, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
1.25 mM Tfb1, 1 mM [U-100% 13C; U-100% 15N] Rad2, 20 mM sodium phosphate, 1 mM EDTA, 1 mM DTT, 100% D2O | 100% D2O
NMR sample composition
1.25 mM Tfb1, 1 mM [U-100% 15N] Rad2, 20 mM sodium phosphate, 1 mM EDTA, 1 mM DTT, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 4Q0R The catalytic core of Rad2 (complex I) Deposited 2014-04-02 | Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: dimeric（2） Consistent with all polymers |
Chain A
2–111（110 aa）
Fragment：enzyme catalytic core, unp residues 2-111, unp residues 732-986
Chain A
732–986（255 aa）
Fragment：enzyme catalytic core, unp residues 2-111, unp residues 732-986
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；291 K；20% (v/v) ethylene glycol, VAPOR DIFFUSION, SITTING DROP, temperature 291K
|
Resolution 2.75 Å R-free 0.310 |
| 4Q0R The catalytic core of Rad2 (complex I) Deposited 2014-04-02 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
2–111（110 aa）
Fragment：enzyme catalytic core, unp residues 2-111, unp residues 732-986
Chain B
732–986（255 aa）
Fragment：enzyme catalytic core, unp residues 2-111, unp residues 732-986
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；291 K；20% (v/v) ethylene glycol, VAPOR DIFFUSION, SITTING DROP, temperature 291K
|
Resolution 2.75 Å R-free 0.310 |
| 4Q0W he catalytic core of Rad2 in complex with DNA substrate (complex II) Deposited 2014-04-02 | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain A
2–111（110 aa）
Fragment：Rad2
Chain A
732–986（255 aa）
Fragment：Rad2
Chain B
2–111（110 aa）
Fragment：Rad2
Chain B
732–986（255 aa）
Fragment：Rad2
|
Not recorded | CA CALCIUM ION × 2 K POTASSIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；291 K；9% (w/v) PEG 20000, 20% (v/v) PEG MME 550, 0.2 M D-glucose, 0.2 M D-mannose, 0.2 M D-galactose, 0.2 M L-fructose, 0.2 M D-xylose, 0.2 M N-acetyl-D-glucosamine, and 0.1 M MOPS/HEPES-Na, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K
|
Resolution 2.10 Å R-free 0.230 |
| 4Q0Z The catalytic core of Rad2 in complex with DNA substrate (complex III) Deposited 2014-04-02 | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain A
2–111（110 aa）
Fragment：Rad2 catalytic core
Chain A
732–986（255 aa）
Fragment：Rad2 catalytic core
Chain B
2–111（110 aa）
Fragment：Rad2 catalytic core
Chain B
732–986（255 aa）
Fragment：Rad2 catalytic core
|
Not recorded | CA CALCIUM ION × 2 K POTASSIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；291 K；10% (w/v) PEG 8000, 20% (v/v) ethylene glycol, 0.2 M D-glucose, 0.2 M D-mannose, 0.2 M D-galactose, 0.2 M L-fructose, 0.2 M D-xylose, 0.2 M N-acetyl-D-glucosamine, and 0.1 M MES/imidazole, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K
|
Resolution 2.40 Å R-free 0.235 |
| 4Q0Z The catalytic core of Rad2 in complex with DNA substrate (complex III) Deposited 2014-04-02 | Assembly 2 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain E
2–111（110 aa）
Fragment：Rad2 catalytic core
Chain E
732–986（255 aa）
Fragment：Rad2 catalytic core
Chain F
2–111（110 aa）
Fragment：Rad2 catalytic core
Chain F
732–986（255 aa）
Fragment：Rad2 catalytic core
|
Not recorded | CA CALCIUM ION × 2 K POTASSIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；291 K；10% (w/v) PEG 8000, 20% (v/v) ethylene glycol, 0.2 M D-glucose, 0.2 M D-mannose, 0.2 M D-galactose, 0.2 M L-fructose, 0.2 M D-xylose, 0.2 M N-acetyl-D-glucosamine, and 0.1 M MES/imidazole, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K
|
Resolution 2.40 Å R-free 0.235 |
| 4Q10 The catalytic core of Rad2 in complex with DNA substrate (complex IV) Deposited 2014-04-02 | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain A
2–111（110 aa）
Fragment：enzyme catalytic core, unp residues 2-111, unp residues 732-986
Chain A
732–986（255 aa）
Fragment：enzyme catalytic core, unp residues 2-111, unp residues 732-986
Chain B
2–111（110 aa）
Fragment：enzyme catalytic core, unp residues 2-111, unp residues 732-986
Chain B
732–986（255 aa）
Fragment：enzyme catalytic core, unp residues 2-111, unp residues 732-986
|
Not recorded | CA CALCIUM ION × 2 K POTASSIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；291 K；8% (w/v) PEG 8000, 18% (v/v) ethylene glycol, 0.2 M D-glucose, 0.2 M D-mannose, 0.2 M D-galactose, 0.2 M L-fructose, 0.2 M D-xylose, 0.2 M N-acetyl-D-glucosamine, and 0.1 M MOPS/HEPES-Na, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K
|
Resolution 2.70 Å R-free 0.284 |