当前蛋白身份：P11142
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差异标签只比较当前检索结果；所有PDB和assembly原始记录仍分别保留。
相关结构差异明细
一行代表一个 PDB 条目中的一个 biological assembly；同一蛋白的多个单体会分别列出。
| PDB 条目 | Assembly / 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与非聚合物 | 实验方法 | 实验环境 | 结构质量 |
|---|---|---|---|---|---|---|---|
| 3AGY Crystal structure of human Hsp40 Hdj1 peptide-binding domain complexed with a C-terminal peptide of Hsp70 提交 2010-04-12 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 5 PDB 声明：pentameric（5） 与蛋白数一致 |
链 C
639–646（8 aa）
片段：UNP residues 639-646
链 D
639–646（8 aa）
片段：UNP residues 639-646
链 F
639–646（8 aa）
片段：UNP residues 639-646
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；18% PEG 3350, 0.1M Na citrate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
分辨率 1.85 Å R-free 0.247 |
| 3AGZ Crystal structure of human Hsp40 Hdj1 peptide-binding domain complexed with a C-terminal peptide of Hsp70 提交 2010-04-12 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 6 PDB 声明：hexameric（6） 与蛋白数一致 |
链 C
639–646（8 aa）
片段：UNP residues 639-646
链 D
639–646（8 aa）
片段：UNP residues 639-646
链 E
639–646（8 aa）
片段：UNP residues 639-646
链 F
639–646（8 aa）
片段：UNP residues 639-646
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 8.8；277 K；18% PEG 3350, 0.1M CHES, pH 8.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
分辨率 2.51 Å R-free 0.269 |
| 3ESK Structure of HOP TPR2A domain in complex with the non-cognate Hsc70 peptide ligand 提交 2008-10-06 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 B
635–646（12 aa）
片段：Hsc70 C-terminal peptide, UNP residues 635-646
|
未记录 | NI NICKEL (II) ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；298 K；TRIS pH 8.5, PEG MME 2000, NiCl2, Xylitol, VAPOR DIFFUSION, temperature 298K
|
分辨率 2.05 Å R-free 0.244 |
| 3FZF Crystal Structure of Hsc70/Bag1 in complex with ATP 提交 2009-01-26 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
4–381（378 aa）
片段：UNP residues 4-381
|
未记录 | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；15% PEG3350, 0.1M Tris buffer, 25mM sodium-potassium tartrate, pH8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 2.20 Å R-free 0.292 |
| 3FZH Crystal Structures of Hsc70/Bag1 in Complex with Small Molecule Inhibitors 提交 2009-01-26 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
4–381（378 aa）
片段：UNP residues 4-381
|
未记录 | 3BH (2R,3R,4S,5R)-2-(6,8-diaminopurin-9-yl)-5-(hydroxymethyl)oxolane-3,4-diol × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；15% PEG3350, 0.1M Tris buffer, 25mM sodium-potassium tartrate, pH8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 2.00 Å R-free 0.272 |
| 3FZK Crystal Structures of Hsc70/Bag1 in Complex with Small Molecule Inhibitors 提交 2009-01-26 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
4–381（378 aa）
片段：UNP residues 4-381
|
未记录 | TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 CL CHLORIDE ION × 1 3BK (2R,3R,4S,5R)-2-[6-amino-8-[(3,4-dichlorophenyl)methylamino]purin-9-yl]-5-(hydroxymethyl)oxolane-3,4-diol × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；15% PEG3350, 0.1M Tris buffer, 25mM sodium-potassium tartrate, pH8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 2.10 Å R-free 0.294 |
| 3FZL Crystal Structures of Hsc70/Bag1 in Complex with Small Molecule Inhibitors 提交 2009-01-26 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
4–381（378 aa）
片段：UNP residues 4-381
|
未记录 | TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 3FD 4-[[(2R,3S,4R,5R)-5-[6-amino-8-[(3,4-dichlorophenyl)methylamino]purin-9-yl]-3,4-dihydroxy-oxolan-2-yl]methoxymethyl]benzonitrile × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；15% PEG3350, 0.1M Tris buffer, 25mM sodium-potassium tartrate, pH8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 2.20 Å R-free 0.304 |
| 3FZM Crystal Structures of Hsc70/Bag1 in Complex with Small Molecule Inhibitors 提交 2009-01-26 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
4–381（378 aa）
片段：UNP residues 4-381
|
未记录 | 3GO 4-[[(2R,3S,4R,5R)-5-[6-amino-8-(quinolin-6-ylmethylamino)purin-9-yl]-3,4-dihydroxy-oxolan-2-yl]methoxymethyl]benzonitri le × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；15% PEG3350, 0.1M Tris buffer, 25mM sodium-potassium tartrate, pH8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 2.30 Å R-free 0.254 |
| 3LDQ Crystal structure of HSC70/BAG1 in complex with small molecule inhibitor 提交 2010-01-13 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
4–381（378 aa）
|
未记录 | 3P1 8-[(quinolin-2-ylmethyl)amino]adenosine × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；15% PEG3350, 0.1M TRIS BUFFER, 25MM SODIUM-POTASSIUM TARTRATE, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 1.90 Å R-free 0.256 |
| 3M3Z Crystal structure of HSC70/BAG1 in complex with small molecule inhibitor 提交 2010-03-10 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
4–381（378 aa）
|
未记录 | 3F5 5'-O-(2-amino-2-oxoethyl)-8-(methylamino)adenosine × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；15% PEG3350, 0.1M TRIS BUFFER, 25MM SODIUM-POTASSIUM TARTRATE, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 2.10 Å R-free 0.293 |
| 4H5N HSC70 NBD with PO4, Na, Cl 提交 2012-09-18 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
2–384（383 aa）
|
未记录 | PO4 PHOSPHATE ION × 5 CL CHLORIDE ION × 1 NA SODIUM ION × 1 GOL GLYCEROL × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8.5；298 K；0.1 M KCl in Tris, 30%PEG 400, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
分辨率 1.86 Å R-free 0.248 |
| 4H5N HSC70 NBD with PO4, Na, Cl 提交 2012-09-18 | Assembly 2 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
2–384（383 aa）
|
未记录 | PO4 PHOSPHATE ION × 6 CL CHLORIDE ION × 1 NA SODIUM ION × 1 GOL GLYCEROL × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8.5；298 K；0.1 M KCl in Tris, 30%PEG 400, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
分辨率 1.86 Å R-free 0.248 |
| 4H5N HSC70 NBD with PO4, Na, Cl 提交 2012-09-18 | Assembly 3 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
2–384（383 aa）
链 B
2–384（383 aa）
|
未记录 | PO4 PHOSPHATE ION × 11 CL CHLORIDE ION × 2 NA SODIUM ION × 2 GOL GLYCEROL × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8.5；298 K；0.1 M KCl in Tris, 30%PEG 400, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
分辨率 1.86 Å R-free 0.248 |
| 4H5R HSC70 NBD with Na, Cl and glycerol 提交 2012-09-18 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
2–384（383 aa）
|
未记录 | NA SODIUM ION × 1 PO4 PHOSPHATE ION × 5 CL CHLORIDE ION × 1 SO4 SULFATE ION × 1 GOL GLYCEROL × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8.5；0.1 M KCl in Tris, 30%PEG 400, pH 8.5, VAPOR DIFFUSION, SITTING DROP
|
分辨率 1.64 Å R-free 0.239 |
| 4H5R HSC70 NBD with Na, Cl and glycerol 提交 2012-09-18 | Assembly 2 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
2–384（383 aa）
|
未记录 | NA SODIUM ION × 1 PO4 PHOSPHATE ION × 2 CL CHLORIDE ION × 1 SO4 SULFATE ION × 2 GOL GLYCEROL × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8.5；0.1 M KCl in Tris, 30%PEG 400, pH 8.5, VAPOR DIFFUSION, SITTING DROP
|
分辨率 1.64 Å R-free 0.239 |
| 4H5R HSC70 NBD with Na, Cl and glycerol 提交 2012-09-18 | Assembly 3 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
2–384（383 aa）
链 B
2–384（383 aa）
|
未记录 | NA SODIUM ION × 2 PO4 PHOSPHATE ION × 7 CL CHLORIDE ION × 2 SO4 SULFATE ION × 3 GOL GLYCEROL × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8.5；0.1 M KCl in Tris, 30%PEG 400, pH 8.5, VAPOR DIFFUSION, SITTING DROP
|
分辨率 1.64 Å R-free 0.239 |
| 4H5T HSC70 NBD with ADP and Mg 提交 2012-09-18 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
2–384（383 aa）
|
未记录 | ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 2 PO4 PHOSPHATE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 9.5；298 K；0.1 M TRIS buffer, 1.5M NaCl, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
分辨率 1.90 Å R-free 0.298 |
| 4H5V HSC70 NBD with Mg 提交 2012-09-18 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
2–384（383 aa）
|
未记录 | SO4 SULFATE ION × 3 MG MAGNESIUM ION × 1 CL CHLORIDE ION × 1 GOL GLYCEROL × 4 ACT ACETATE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 5.6；298 K；0.1 M ammonium sulfate (AS) in 0.1 M MES buffer, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
分辨率 1.75 Å R-free 0.215 |
| 4H5W HSC70 NBD with betaine 提交 2012-09-19 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
2–384（383 aa）
|
未记录 | MG MAGNESIUM ION × 1 PO4 PHOSPHATE ION × 2 BET TRIMETHYL GLYCINE × 1 ACT ACETATE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8.5；298 K；0.2 M Ammonium Acetate, 0.1 M Tris, 25% PEG3350, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
分辨率 1.94 Å R-free 0.253 |
| 4H5W HSC70 NBD with betaine 提交 2012-09-19 | Assembly 2 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
2–384（383 aa）
|
未记录 | MG MAGNESIUM ION × 1 PO4 PHOSPHATE ION × 2 BET TRIMETHYL GLYCINE × 1 ACT ACETATE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8.5；298 K；0.2 M Ammonium Acetate, 0.1 M Tris, 25% PEG3350, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
分辨率 1.94 Å R-free 0.253 |
| 4HWI Crystal structure of ATBAG1 in complex with HSP70 提交 2012-11-07 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
5–381（377 aa）
片段：HSP70 ATPase domain (UNP residues 5-381)
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 7.5；277 K；0.02 M citric acid / 0.08 M Bis-Tris propane, pH 8.8, 20% PEG3350, VAPOR DIFFUSION, temperature 277K
|
分辨率 2.27 Å R-free 0.259 |
| 4KBQ Structure of the CHIP-TPR domain in complex with the Hsc70 Lid-Tail domains 提交 2013-04-23 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 C
541–646（106 aa）
片段：Lid-Tail (delta626-638)
|
突变：delta(626-638) deletion mutant | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；1.7M ammonium citrate, 0.1M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 2.91 Å R-free 0.263 |
| 4KBQ Structure of the CHIP-TPR domain in complex with the Hsc70 Lid-Tail domains 提交 2013-04-23 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 D
541–646（106 aa）
片段：Lid-Tail (delta626-638)
|
突变：delta(626-638) deletion mutant | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；1.7M ammonium citrate, 0.1M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 2.91 Å R-free 0.263 |
| 5AQF Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
1–381（381 aa）
片段：NUCLEOTIDE BINDING DOMAIN, RESIDUES 1-381
|
未记录 | ADN ADENOSINE × 1 GOL GLYCEROL × 8 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；HSC70/BAG1 COMPLEX AT 10 MG/ML INCUBATED WITH 5 MM ADENOSINE, MIXED 1:1 WITH 16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 1.88 Å R-free 0.208 |
| 5AQF Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 C
1–381（381 aa）
片段：NUCLEOTIDE BINDING DOMAIN, RESIDUES 1-381
|
未记录 | ADN ADENOSINE × 1 GOL GLYCEROL × 4 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；HSC70/BAG1 COMPLEX AT 10 MG/ML INCUBATED WITH 5 MM ADENOSINE, MIXED 1:1 WITH 16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 1.88 Å R-free 0.208 |
| 5AQG Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
1–381（381 aa）
片段：NUCLEOTIDE BINDING DOMAIN, RESIDUES 1-381
|
未记录 | ZJB (2R,3R,4S,5R)-2-(3-AMINO-5-METHYL-1,4,5,6,8-PENTAAZAACENAPHTHYLEN-1(5H)-YL)-5-(HYDROXYMETHYL)TETRAHYDROFURAN-3,4-DIOL × 1 GOL GLYCEROL × 2 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 2.24 Å R-free 0.222 |
| 5AQG Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 C
1–381（381 aa）
片段：NUCLEOTIDE BINDING DOMAIN, RESIDUES 1-381
|
未记录 | ZJB (2R,3R,4S,5R)-2-(3-AMINO-5-METHYL-1,4,5,6,8-PENTAAZAACENAPHTHYLEN-1(5H)-YL)-5-(HYDROXYMETHYL)TETRAHYDROFURAN-3,4-DIOL × 1 GOL GLYCEROL × 6 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 2.24 Å R-free 0.222 |
| 5AQG Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 3 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 E
1–381（381 aa）
片段：NUCLEOTIDE BINDING DOMAIN, RESIDUES 1-381
|
未记录 | ZJB (2R,3R,4S,5R)-2-(3-AMINO-5-METHYL-1,4,5,6,8-PENTAAZAACENAPHTHYLEN-1(5H)-YL)-5-(HYDROXYMETHYL)TETRAHYDROFURAN-3,4-DIOL × 1 GOL GLYCEROL × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 2.24 Å R-free 0.222 |
| 5AQH Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
1–381（381 aa）
片段：NUCLEOTIDE BINDING DOMAIN, RESIDUES 1-381
|
未记录 | ZVO 5-methyl-1,5-dihydro-1,4,5,6,8-pentaazaacenaphthylen-3-amine × 1 GOL GLYCEROL × 7 DMS DIMETHYL SULFOXIDE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 2.00 Å R-free 0.228 |
| 5AQI Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
1–381（381 aa）
片段：NUCLEOTIDE BINDING DOMAIN, RESIDUES 1-381
|
未记录 | DMS DIMETHYL SULFOXIDE × 2 GOL GLYCEROL × 7 ADE ADENINE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 1.98 Å R-free 0.242 |
| 5AQI Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 C
1–381（381 aa）
片段：NUCLEOTIDE BINDING DOMAIN, RESIDUES 1-381
|
未记录 | DMS DIMETHYL SULFOXIDE × 1 GOL GLYCEROL × 4 ADE ADENINE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 1.98 Å R-free 0.242 |
| 5AQJ Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 E
1–381（381 aa）
片段：NUCLEOTIDE BINDING DOMAIN, RESIDUES 1-381
|
未记录 | CL CHLORIDE ION × 1 Q88 9H-purine-6,8-diamine × 1 GOL GLYCEROL × 5 DMS DIMETHYL SULFOXIDE × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 1.96 Å R-free 0.221 |
| 5AQJ Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
1–381（381 aa）
片段：NUCLEOTIDE BINDING DOMAIN, RESIDUES 1-381
|
未记录 | CL CHLORIDE ION × 1 Q88 9H-purine-6,8-diamine × 1 GOL GLYCEROL × 3 DMS DIMETHYL SULFOXIDE × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 1.96 Å R-free 0.221 |
| 5AQJ Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 3 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 C
1–381（381 aa）
片段：NUCLEOTIDE BINDING DOMAIN, RESIDUES 1-381
|
未记录 | CL CHLORIDE ION × 1 Q88 9H-purine-6,8-diamine × 1 GOL GLYCEROL × 8 DMS DIMETHYL SULFOXIDE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 1.96 Å R-free 0.221 |
| 5AQK Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
1–381（381 aa）
片段：NUCLEOTIDE BINDING DOMAIN, RESIDUES 1-381
|
未记录 | GDP GUANOSINE-5'-DIPHOSPHATE × 2 GOL GLYCEROL × 3 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 DMS DIMETHYL SULFOXIDE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5, 25% (V/V) GLYCEROL AND 100 MM GDP FROM 1 M STOCK AT PH 7.5
|
分辨率 2.09 Å R-free 0.232 |
| 5AQL Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
1–381（381 aa）
片段：NUCLEOTIDE BINDING DOMAIN, RESIDUES 1-381
|
突变：YES | TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 2 GOL GLYCEROL × 3 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 1.69 Å R-free 0.197 |
| 5AQL Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 C
1–381（381 aa）
片段：NUCLEOTIDE BINDING DOMAIN, RESIDUES 1-381
|
突变：YES | TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 2 GOL GLYCEROL × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 1.69 Å R-free 0.197 |
| 5AQM Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
1–381（381 aa）
片段：NUCLEOTIDE BINDING DOMAIN, RESIDUES 1-381
|
突变：YES | TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 2 GOL GLYCEROL × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 1.63 Å R-free 0.195 |
| 5AQM Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 C
1–381（381 aa）
片段：NUCLEOTIDE BINDING DOMAIN, RESIDUES 1-381
|
突变：YES | TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 2 GOL GLYCEROL × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 1.63 Å R-free 0.195 |
| 5AQN Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
1–381（381 aa）
片段：NUCLEOTIDE BINDING DOMAIN RESIDUES 1-381
|
未记录 | JG8 BENZOFURO[3,2-D]PYRIMIDIN-4(3H)-ONE × 1 GOL GLYCEROL × 4 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 DMS DIMETHYL SULFOXIDE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 2.45 Å R-free 0.228 |
| 5AQN Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 C
1–381（381 aa）
片段：NUCLEOTIDE BINDING DOMAIN RESIDUES 1-381
|
未记录 | JG8 BENZOFURO[3,2-D]PYRIMIDIN-4(3H)-ONE × 1 GOL GLYCEROL × 3 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 DMS DIMETHYL SULFOXIDE × 1 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 2.45 Å R-free 0.228 |
| 5AQN Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 3 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 E
1–381（381 aa）
片段：NUCLEOTIDE BINDING DOMAIN RESIDUES 1-381
|
未记录 | JG8 BENZOFURO[3,2-D]PYRIMIDIN-4(3H)-ONE × 1 GOL GLYCEROL × 2 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 DMS DIMETHYL SULFOXIDE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 2.45 Å R-free 0.228 |
| 5AQO Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
1–381（381 aa）
片段：NUCLEOTIDE BINDING DOMAIN RESIDUES 1-381
|
未记录 | GOL GLYCEROL × 6 CWS 6-METHYLQUINAZOLIN-4-AMINE × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 DMS DIMETHYL SULFOXIDE × 1 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 2.12 Å R-free 0.210 |
| 5AQO Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 C
1–381（381 aa）
片段：NUCLEOTIDE BINDING DOMAIN RESIDUES 1-381
|
未记录 | GOL GLYCEROL × 10 CWS 6-METHYLQUINAZOLIN-4-AMINE × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 DMS DIMETHYL SULFOXIDE × 1 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 2.12 Å R-free 0.210 |
| 5AQO Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 3 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 E
1–381（381 aa）
片段：NUCLEOTIDE BINDING DOMAIN RESIDUES 1-381
|
未记录 | GOL GLYCEROL × 5 CWS 6-METHYLQUINAZOLIN-4-AMINE × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 DMS DIMETHYL SULFOXIDE × 1 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 2.12 Å R-free 0.210 |
| 5AQP Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 C
1–381（381 aa）
片段：NUCLEOTIDE BINDING DOMAIN, UNP RESIDUES 1-381
|
未记录 | 1LQ quinazolin-4-amine × 1 GOL GLYCEROL × 8 DMS DIMETHYL SULFOXIDE × 2 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 2.08 Å R-free 0.209 |
| 5AQP Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 E
1–381（381 aa）
片段：NUCLEOTIDE BINDING DOMAIN, UNP RESIDUES 1-381
|
未记录 | 1LQ quinazolin-4-amine × 1 GOL GLYCEROL × 4 DMS DIMETHYL SULFOXIDE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 2.08 Å R-free 0.209 |
| 5AQP Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 3 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
1–381（381 aa）
片段：NUCLEOTIDE BINDING DOMAIN, UNP RESIDUES 1-381
|
未记录 | 1LQ quinazolin-4-amine × 1 GOL GLYCEROL × 2 DMS DIMETHYL SULFOXIDE × 2 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 2.08 Å R-free 0.209 |
| 5AQQ Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 E
1–381（381 aa）
片段：NUCLEOTIDE BINDING DOMAIN, UNP RESIDUES 1-381
|
未记录 | BBW 7-methylquinazolin-4-amine × 1 DMS DIMETHYL SULFOXIDE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 2.72 Å R-free 0.241 |
| 5AQQ Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
1–381（381 aa）
片段：NUCLEOTIDE BINDING DOMAIN, UNP RESIDUES 1-381
|
未记录 | BBW 7-methylquinazolin-4-amine × 1 DMS DIMETHYL SULFOXIDE × 1 GOL GLYCEROL × 2 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 2.72 Å R-free 0.241 |
| 5AQQ Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 3 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 C
1–381（381 aa）
片段：NUCLEOTIDE BINDING DOMAIN, UNP RESIDUES 1-381
|
未记录 | BBW 7-methylquinazolin-4-amine × 1 DMS DIMETHYL SULFOXIDE × 1 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 2.72 Å R-free 0.241 |
| 5AQR Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
1–381（381 aa）
片段：NUCLEOTIDE BINDING DOMAIN RESIDUES 1-381
|
未记录 | TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 N8Y 6-methoxyquinazolin-4-amine × 1 GOL GLYCEROL × 11 DMS DIMETHYL SULFOXIDE × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 1.91 Å R-free 0.236 |
| 5AQR Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 C
1–381（381 aa）
片段：NUCLEOTIDE BINDING DOMAIN RESIDUES 1-381
|
未记录 | TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 N8Y 6-methoxyquinazolin-4-amine × 1 GOL GLYCEROL × 5 DMS DIMETHYL SULFOXIDE × 1 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 1.91 Å R-free 0.236 |
| 5AQR Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 3 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 E
1–381（381 aa）
片段：NUCLEOTIDE BINDING DOMAIN RESIDUES 1-381
|
未记录 | TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 N8Y 6-methoxyquinazolin-4-amine × 1 GOL GLYCEROL × 2 DMS DIMETHYL SULFOXIDE × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 1.91 Å R-free 0.236 |
| 5AQS Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 C
1–381（381 aa）
片段：NUCLEOTIDE BINDING DOMAIN RESIDUES 1-381
|
未记录 | 1SQ ISOQUINOLIN-1-AMINE × 1 GOL GLYCEROL × 3 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 2.00 Å R-free 0.261 |
| 5AQS Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
1–381（381 aa）
片段：NUCLEOTIDE BINDING DOMAIN RESIDUES 1-381
|
未记录 | 1SQ ISOQUINOLIN-1-AMINE × 1 GOL GLYCEROL × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 2.00 Å R-free 0.261 |
| 5AQT Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
1–381（381 aa）
片段：NUCLEOTIDE BINDING DOMAIN, UNP RESIDUES 1-381
|
未记录 | 5P7 (1S,2R,3R,5R)-3-(hydroxymethyl)-5-(quinazolin-4-ylamino)cyclopentane-1,2-diol × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 DMS DIMETHYL SULFOXIDE × 2 GOL GLYCEROL × 6 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 1.90 Å R-free 0.222 |
| 5AQU Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
1–381（381 aa）
片段：NUCLEOTIDE BINDING DOMAIN, UNP RESIDUES 1-381
|
未记录 | GOL GLYCEROL × 9 UX0 (1S,2R,3R,5R)-3-(HYDROXYMETHYL)-5-((5-METHOXYQUINAZOLIN-4-YL)AMINO)CYCLOPENTANE-1,2-DIOL × 1 DMS DIMETHYL SULFOXIDE × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 1.92 Å R-free 0.201 |
| 5AQV Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
1–381（381 aa）
片段：NUCLEOTIDE BINDING DOMAIN, RESIDUES 1-381
|
未记录 | KC7 (1R,2S,3R,5R)-3-((5-(benzyloxy)quinazolin-4-yl)amino)-5-(hydroxymethyl)cyclopentane-1,2-diol × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 GOL GLYCEROL × 7 DMS DIMETHYL SULFOXIDE × 3 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 1.75 Å R-free 0.197 |
| 6B1I Disrupted hydrogen bond network impairs ATPase activity in an Hsc70 cysteine mutant 提交 2017-09-18 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
5–381（377 aa）
|
未记录 | MG MAGNESIUM ION × 1 PO4 PHOSPHATE ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 GOL GLYCEROL × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；293.15 K；NaCl, Tris, PEG 3350, MgCl2, ATP
|
分辨率 2.30 Å R-free 0.242 |
| 6B1I Disrupted hydrogen bond network impairs ATPase activity in an Hsc70 cysteine mutant 提交 2017-09-18 | Assembly 2 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
5–381（377 aa）
|
未记录 | MG MAGNESIUM ION × 1 PO4 PHOSPHATE ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；293.15 K；NaCl, Tris, PEG 3350, MgCl2, ATP
|
分辨率 2.30 Å R-free 0.242 |
| 6B1M Disrupted hydrogen bond network impairs ATPase activity in an Hsc70 cysteine mutant 提交 2017-09-18 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
5–381（377 aa）
|
突变：C17W | ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；293.15 K；NaCl, Tris, PEG 3350, MgCl2, AppNHp
|
分辨率 1.90 Å R-free 0.230 |
| 6B1M Disrupted hydrogen bond network impairs ATPase activity in an Hsc70 cysteine mutant 提交 2017-09-18 | Assembly 2 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
5–381（377 aa）
|
突变：C17W | ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 MG MAGNESIUM ION × 1 GOL GLYCEROL × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；293.15 K；NaCl, Tris, PEG 3350, MgCl2, AppNHp
|
分辨率 1.90 Å R-free 0.230 |
| 6B1N Disrupted hydrogen bond network impairs ATPase activity in an Hsc70 cysteine mutant 提交 2017-09-18 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
5–381（377 aa）
|
突变：C17W | ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GOL GLYCEROL × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；293.15 K；NaCl, Tris, PEG 3350, MgCl2, ADP
|
分辨率 1.80 Å R-free 0.226 |
| 6B1N Disrupted hydrogen bond network impairs ATPase activity in an Hsc70 cysteine mutant 提交 2017-09-18 | Assembly 2 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
5–381（377 aa）
|
突变：C17W | ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GOL GLYCEROL × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；293.15 K；NaCl, Tris, PEG 3350, MgCl2, ADP
|
分辨率 1.80 Å R-free 0.226 |
| 6ZYJ Crystal structure of Hsc70 ATPase domain in complex with ADP and calcium 提交 2020-08-01 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
5–384（380 aa）
|
未记录 | ACT ACETATE ION × 1 CA CALCIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；293 K；24% PEG3350,
0.2M Ca acetate
|
分辨率 1.85 Å R-free 0.247 |
| 6ZYJ Crystal structure of Hsc70 ATPase domain in complex with ADP and calcium 提交 2020-08-01 | Assembly 2 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
5–384（380 aa）
|
未记录 | ACT ACETATE ION × 1 CA CALCIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；293 K；24% PEG3350,
0.2M Ca acetate
|
分辨率 1.85 Å R-free 0.247 |