当前蛋白身份：P14735
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差异标签只比较当前检索结果；所有PDB和assembly原始记录仍分别保留。
相关结构差异明细
一行代表一个 PDB 条目中的一个 biological assembly；同一蛋白的多个单体会分别列出。
| PDB 条目 | Assembly / 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与非聚合物 | 实验方法 | 实验环境 | 结构质量 |
|---|---|---|---|---|---|---|---|
| 2G47 Crystal structure of human insulin-degrading enzyme in complex with amyloid-beta (1-40) 提交 2006-02-21 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
41–1018（978 aa）
|
突变：E111Q | DIO 1,4-DIETHYLENE DIOXIDE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；10% PEGMME 5000, 0.1M HEPES, 10% diaoxane, 5% tacsimate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
分辨率 2.10 Å R-free 0.223 |
| 2G47 Crystal structure of human insulin-degrading enzyme in complex with amyloid-beta (1-40) 提交 2006-02-21 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 B
41–1018（978 aa）
|
突变：E111Q | DIO 1,4-DIETHYLENE DIOXIDE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；10% PEGMME 5000, 0.1M HEPES, 10% diaoxane, 5% tacsimate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
分辨率 2.10 Å R-free 0.223 |
| 2JG4 Substrate-free IDE structure in its closed conformation 提交 2007-02-07 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
42–1018（977 aa）
片段：RESIDUES 42-1018
|
突变：YES | ZN ZINC ION × 1 DIO 1,4-DIETHYLENE DIOXIDE × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；10% PEGMME 5000, 0.1M HEPES, 10%, DIAOXANE, 5% TACSIMATE, PH 7.0, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K
|
分辨率 2.80 Å R-free 0.227 |
| 2JG4 Substrate-free IDE structure in its closed conformation 提交 2007-02-07 | Assembly 2 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
42–1018（977 aa）
片段：RESIDUES 42-1018
|
突变：YES | ZN ZINC ION × 1 DIO 1,4-DIETHYLENE DIOXIDE × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；10% PEGMME 5000, 0.1M HEPES, 10%, DIAOXANE, 5% TACSIMATE, PH 7.0, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K
|
分辨率 2.80 Å R-free 0.227 |
| 2WBY Crystal structure of human insulin-degrading enzyme in complex with insulin 提交 2009-03-06 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 3 PDB 声明：trimeric（3） 与蛋白数一致 |
链 A
42–1019（978 aa）
片段：RESIDUES 42-1019
|
突变：YES | ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 7；10-13% PEG MME 5000, 100 MM HEPES PH 7.0, 4-14% TACSIMATE, 10% DIOXANE
|
分辨率 2.60 Å R-free 0.218 |
| 2WBY Crystal structure of human insulin-degrading enzyme in complex with insulin 提交 2009-03-06 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 3 PDB 声明：trimeric（3） 与蛋白数一致 |
链 B
42–1019（978 aa）
片段：RESIDUES 42-1019
|
突变：YES | ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 7；10-13% PEG MME 5000, 100 MM HEPES PH 7.0, 4-14% TACSIMATE, 10% DIOXANE
|
分辨率 2.60 Å R-free 0.218 |
| 2WC0 crystal structure of human insulin degrading enzyme in complex with iodinated insulin 提交 2009-03-06 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 3 PDB 声明：trimeric（3） 与蛋白数一致 |
链 A
42–1019（978 aa）
片段：RESIDUES 42-1019
|
突变：YES | ZN ZINC ION × 1 DIO 1,4-DIETHYLENE DIOXIDE × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 7；pH 7
|
分辨率 2.80 Å R-free 0.220 |
| 2WC0 crystal structure of human insulin degrading enzyme in complex with iodinated insulin 提交 2009-03-06 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 3 PDB 声明：trimeric（3） 与蛋白数一致 |
链 B
42–1019（978 aa）
片段：RESIDUES 42-1019
|
突变：YES | ZN ZINC ION × 1 DIO 1,4-DIETHYLENE DIOXIDE × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 7；pH 7
|
分辨率 2.80 Å R-free 0.220 |
| 2WK3 Crystal structure of human insulin-degrading enzyme in complex with amyloid-beta (1-42) 提交 2009-06-05 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 B
1–1019（1019 aa）
|
突变：YES | ZN ZINC ION × 1 | X-RAY DIFFRACTION | mmCIF 未提供已读取条件 | 分辨率 2.59 Å R-free 0.232 |
| 2WK3 Crystal structure of human insulin-degrading enzyme in complex with amyloid-beta (1-42) 提交 2009-06-05 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
1–1019（1019 aa）
|
突变：YES | ZN ZINC ION × 1 | X-RAY DIFFRACTION | mmCIF 未提供已读取条件 | 分辨率 2.59 Å R-free 0.232 |
| 2YPU human insulin degrading enzyme E111Q in complex with inhibitor compound 41367 提交 2012-11-01 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
42–1019（978 aa）
片段：RESIDUES 42-1019
|
突变：YES | ZN ZINC ION × 1 I41 2-[[2-[[(2S)-3-(3H-IMIDAZOL-4-YL)-1-METHOXY-1-OXO-PROPAN-2-YL]AMINO]-2-OXO-ETHYL]-(PHENYLMETHYL)AMINO]ETHANOIC ACID × 1 | X-RAY DIFFRACTION | mmCIF 未提供已读取条件 | 分辨率 2.80 Å R-free 0.225 |
| 2YPU human insulin degrading enzyme E111Q in complex with inhibitor compound 41367 提交 2012-11-01 | Assembly 2 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
42–1019（978 aa）
片段：RESIDUES 42-1019
|
突变：YES | ZN ZINC ION × 1 I41 2-[[2-[[(2S)-3-(3H-IMIDAZOL-4-YL)-1-METHOXY-1-OXO-PROPAN-2-YL]AMINO]-2-OXO-ETHYL]-(PHENYLMETHYL)AMINO]ETHANOIC ACID × 1 | X-RAY DIFFRACTION | mmCIF 未提供已读取条件 | 分辨率 2.80 Å R-free 0.225 |
| 3CWW Crystal Structure of IDE-bradykinin complex 提交 2008-04-23 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 A
42–1019（978 aa）
链 B
42–1019（978 aa）
|
突变：C110L, E111Q, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966N, C974A 突变：C110L, E111Q, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966N, C974A | ZN ZINC ION × 2 ACT ACETATE ION × 4 DIO 1,4-DIETHYLENE DIOXIDE × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 7；291 K；13% PEGMME 5000, 100mM HEPES, pH 7.0, 10%, Tacsimate, 10% dioxane, vapor diffusion, temperature 291K
|
分辨率 1.96 Å R-free 0.208 |
| 3E4A Human IDE-inhibitor complex at 2.6 angstrom resolution 提交 2008-08-11 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
1–1019（1019 aa）
|
突变：C110L, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966N, C974A | ZN ZINC ION × 1 QIX N~2~-[(2R)-4-(HYDROXYAMINO)-2-(2-NAPHTHYLMETHYL)-4-OXOBUTANOYL]-L-ARGINYLGLYCYLGLYCINAMIDE × 1 DIO 1,4-DIETHYLENE DIOXIDE × 2 ACY ACETIC ACID × 8 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；291.5 K；PEG MME 5000, HEPES PH 7.0, TACSIMATE, DIOXANE, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 291K, pH 7.00, temperature 291.5K
|
分辨率 2.60 Å R-free 0.225 |
| 3E4A Human IDE-inhibitor complex at 2.6 angstrom resolution 提交 2008-08-11 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 B
1–1019（1019 aa）
|
突变：C110L, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966N, C974A | ZN ZINC ION × 1 QIX N~2~-[(2R)-4-(HYDROXYAMINO)-2-(2-NAPHTHYLMETHYL)-4-OXOBUTANOYL]-L-ARGINYLGLYCYLGLYCINAMIDE × 1 DIO 1,4-DIETHYLENE DIOXIDE × 5 ACY ACETIC ACID × 6 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；291.5 K；PEG MME 5000, HEPES PH 7.0, TACSIMATE, DIOXANE, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 291K, pH 7.00, temperature 291.5K
|
分辨率 2.60 Å R-free 0.225 |
| 3E50 Crystal structure of human insulin degrading enzyme in complex with transforming growth factor-alpha 提交 2008-08-12 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
42–1019（978 aa）
片段：UNP residues 42-1019
|
突变：E111Q | ZN ZINC ION × 1 | X-RAY DIFFRACTION | mmCIF 未提供已读取条件 | 分辨率 2.30 Å R-free 0.231 |
| 3E50 Crystal structure of human insulin degrading enzyme in complex with transforming growth factor-alpha 提交 2008-08-12 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 B
42–1019（978 aa）
片段：UNP residues 42-1019
|
突变：E111Q | ZN ZINC ION × 1 | X-RAY DIFFRACTION | mmCIF 未提供已读取条件 | 分辨率 2.30 Å R-free 0.231 |
| 3H44 Crystal Structure of Insulin Degrading Enzyme in Complex with macrophage inflammatory protein 1 alpha 提交 2009-04-17 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 A
42–1019（978 aa）
片段：residues 42-1019
链 B
42–1019（978 aa）
片段：residues 42-1019
|
突变：C110L, E111Q, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966N, C974A 突变：C110L, E111Q, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966N, C974A | DIO 1,4-DIETHYLENE DIOXIDE × 6 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；291 K；13% PEGMME 5000, 100mM HEPES, pH 7.0, 10%, Tacsimate, 10% dioxane, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
分辨率 3.00 Å R-free 0.237 |
| 3H44 Crystal Structure of Insulin Degrading Enzyme in Complex with macrophage inflammatory protein 1 alpha 提交 2009-04-17 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 A
42–1019（978 aa）
片段：residues 42-1019
链 B
42–1019（978 aa）
片段：residues 42-1019
|
突变：C110L, E111Q, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966N, C974A 突变：C110L, E111Q, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966N, C974A | DIO 1,4-DIETHYLENE DIOXIDE × 6 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；291 K；13% PEGMME 5000, 100mM HEPES, pH 7.0, 10%, Tacsimate, 10% dioxane, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
分辨率 3.00 Å R-free 0.237 |
| 3H44 Crystal Structure of Insulin Degrading Enzyme in Complex with macrophage inflammatory protein 1 alpha 提交 2009-04-17 | Assembly 3 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
42–1019（978 aa）
片段：residues 42-1019
|
突变：C110L, E111Q, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966N, C974A | DIO 1,4-DIETHYLENE DIOXIDE × 3 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；291 K；13% PEGMME 5000, 100mM HEPES, pH 7.0, 10%, Tacsimate, 10% dioxane, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
分辨率 3.00 Å R-free 0.237 |
| 3H44 Crystal Structure of Insulin Degrading Enzyme in Complex with macrophage inflammatory protein 1 alpha 提交 2009-04-17 | Assembly 4 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 B
42–1019（978 aa）
片段：residues 42-1019
|
突变：C110L, E111Q, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966N, C974A | DIO 1,4-DIETHYLENE DIOXIDE × 3 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；291 K；13% PEGMME 5000, 100mM HEPES, pH 7.0, 10%, Tacsimate, 10% dioxane, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
分辨率 3.00 Å R-free 0.237 |
| 3HGZ Crystal structure of human insulin-degrading enzyme in complex with amylin 提交 2009-05-14 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
43–1011（969 aa）
|
突变：C110L, E111Q, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966N, C974A | ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；291 K；pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
分辨率 2.91 Å R-free 0.224 |
| 3HGZ Crystal structure of human insulin-degrading enzyme in complex with amylin 提交 2009-05-14 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 B
43–1011（969 aa）
|
突变：C110L, E111Q, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966N, C974A | ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；291 K；pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
分辨率 2.91 Å R-free 0.224 |
| 3N56 Crystal Structure of human Insulin-degrading enzyme (IDE) in complex with human B-type natriuretic peptide (BNP) 提交 2010-05-24 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
42–1019（978 aa）
片段：UNP residues 42-1019
|
突变：C110L, E111Q, C171S, C178S, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966N, C974A | ZN ZINC ION × 1 DIO 1,4-DIETHYLENE DIOXIDE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；291 K；13% PEGMME-5000, 10% TACSIMATE, 10% DIOXANE, 100 mM Na-HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
分辨率 3.10 Å R-free 0.243 |
| 3N56 Crystal Structure of human Insulin-degrading enzyme (IDE) in complex with human B-type natriuretic peptide (BNP) 提交 2010-05-24 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 B
42–1019（978 aa）
片段：UNP residues 42-1019
|
突变：C110L, E111Q, C171S, C178S, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966N, C974A | ZN ZINC ION × 1 DIO 1,4-DIETHYLENE DIOXIDE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；291 K；13% PEGMME-5000, 10% TACSIMATE, 10% DIOXANE, 100 mM Na-HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
分辨率 3.10 Å R-free 0.243 |
| 3N57 Crystal Structure of human Insulin-degrading enzyme (IDE) in complex with human atrial natriuretic peptide (ANP) 提交 2010-05-24 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 A
42–1019（978 aa）
片段：UNP residues 42-1019
链 B
42–1019（978 aa）
片段：UNP residues 42-1019
|
突变：C110L, E111Q, C171S, C178S, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966N, C974A 突变：C110L, E111Q, C171S, C178S, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966N, C974A | ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；291 K；13% PEGMME-5000, 10% TACSIMATE, 10% DIOXANE, 100 mM Na-HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
分辨率 3.03 Å R-free 0.242 |
| 3OFI Crystal structure of human insulin-degrading enzyme in complex with ubiquitin 提交 2010-08-15 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
42–1019（978 aa）
|
未记录 | ZN ZINC ION × 1 DIO 1,4-DIETHYLENE DIOXIDE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；291 K；10-13% PEG MME 5000, 100 MM HEPES PH 7.0. 4-14% Tacsimate, 10% Dioxane, vapor diffusion, hanging drop, temperature 291K
|
分辨率 2.35 Å R-free 0.240 |
| 3OFI Crystal structure of human insulin-degrading enzyme in complex with ubiquitin 提交 2010-08-15 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 B
42–1019（978 aa）
|
未记录 | ZN ZINC ION × 1 DIO 1,4-DIETHYLENE DIOXIDE × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；291 K；10-13% PEG MME 5000, 100 MM HEPES PH 7.0. 4-14% Tacsimate, 10% Dioxane, vapor diffusion, hanging drop, temperature 291K
|
分辨率 2.35 Å R-free 0.240 |
| 3QZ2 The structure of cysteine-free human insulin degrading enzyme 提交 2011-03-04 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
42–1019（978 aa）
片段：UNP residues 42-1019
链 B
42–1019（978 aa）
片段：UNP residues 42-1019
|
未记录 | ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；10-13% PEG MME 5000, 100 mM HEPES pH 7.0, 4-14% Tacsimate, 10% dioxane, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
分辨率 3.20 Å R-free 0.241 |
| 4DTT Crystal structure of human insulin degrading enzyme (ide) in complex with compund 41367 提交 2012-02-21 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
42–1019（978 aa）
片段：UNP RESIDUES 42-1019
|
突变：C110L,C171S,C178A,C257V,C414L,C573N,C590S,C789S,C812A,C819A,C904S,C966N,C974A | ZN ZINC ION × 1 I41 2-[[2-[[(2S)-3-(3H-IMIDAZOL-4-YL)-1-METHOXY-1-OXO-PROPAN-2-YL]AMINO]-2-OXO-ETHYL]-(PHENYLMETHYL)AMINO]ETHANOIC ACID × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 7；298 K；10-13% PEG MME 5000, 100 MM HEPES PH 7.0, 4-14% TACSIMATE, 10% DIOXANE, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K
|
分辨率 3.22 Å R-free 0.242 |
| 4DTT Crystal structure of human insulin degrading enzyme (ide) in complex with compund 41367 提交 2012-02-21 | Assembly 2 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
42–1019（978 aa）
片段：UNP RESIDUES 42-1019
|
突变：C110L,C171S,C178A,C257V,C414L,C573N,C590S,C789S,C812A,C819A,C904S,C966N,C974A | ZN ZINC ION × 1 I41 2-[[2-[[(2S)-3-(3H-IMIDAZOL-4-YL)-1-METHOXY-1-OXO-PROPAN-2-YL]AMINO]-2-OXO-ETHYL]-(PHENYLMETHYL)AMINO]ETHANOIC ACID × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 7；298 K；10-13% PEG MME 5000, 100 MM HEPES PH 7.0, 4-14% TACSIMATE, 10% DIOXANE, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K
|
分辨率 3.22 Å R-free 0.242 |
| 4DTT Crystal structure of human insulin degrading enzyme (ide) in complex with compund 41367 提交 2012-02-21 | Assembly 3 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
42–1019（978 aa）
片段：UNP RESIDUES 42-1019
链 B
42–1019（978 aa）
片段：UNP RESIDUES 42-1019
|
突变：C110L,C171S,C178A,C257V,C414L,C573N,C590S,C789S,C812A,C819A,C904S,C966N,C974A 突变：C110L,C171S,C178A,C257V,C414L,C573N,C590S,C789S,C812A,C819A,C904S,C966N,C974A | ZN ZINC ION × 2 I41 2-[[2-[[(2S)-3-(3H-IMIDAZOL-4-YL)-1-METHOXY-1-OXO-PROPAN-2-YL]AMINO]-2-OXO-ETHYL]-(PHENYLMETHYL)AMINO]ETHANOIC ACID × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 7；298 K；10-13% PEG MME 5000, 100 MM HEPES PH 7.0, 4-14% TACSIMATE, 10% DIOXANE, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K
|
分辨率 3.22 Å R-free 0.242 |
| 4DWK Structure of cystein free insulin degrading enzyme with compound bdm41671 ((s)-2-{2-[carboxymethyl-(3-phenyl-propyl)-amino]-acetylamino}-3-(1h-imidazol-4-yl)-propionic acid methyl ester) 提交 2012-02-24 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
42–1019（978 aa）
片段：UNP RESIDUES 42-1019
|
突变：C110L,E111Q,C171S,C178A,C257V,C414L,C573N,C590S,C789S,C812A,C819A,C904S,C966N,C974A | MGK methyl N-(carboxymethyl)-N-(3-phenylpropyl)glycyl-L-histidinate × 1 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；287 K；10-13% PEG MME 5000, 100 MM HEPES PH 7.0, 4-14% TACSIMATE, 10% DIOXANE, VAPOR DIFFUSION, HANGING DROP, temperature 287K
|
分辨率 3.00 Å R-free 0.227 |
| 4DWK Structure of cystein free insulin degrading enzyme with compound bdm41671 ((s)-2-{2-[carboxymethyl-(3-phenyl-propyl)-amino]-acetylamino}-3-(1h-imidazol-4-yl)-propionic acid methyl ester) 提交 2012-02-24 | Assembly 2 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
42–1019（978 aa）
片段：UNP RESIDUES 42-1019
|
突变：C110L,E111Q,C171S,C178A,C257V,C414L,C573N,C590S,C789S,C812A,C819A,C904S,C966N,C974A | MGK methyl N-(carboxymethyl)-N-(3-phenylpropyl)glycyl-L-histidinate × 1 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；287 K；10-13% PEG MME 5000, 100 MM HEPES PH 7.0, 4-14% TACSIMATE, 10% DIOXANE, VAPOR DIFFUSION, HANGING DROP, temperature 287K
|
分辨率 3.00 Å R-free 0.227 |
| 4DWK Structure of cystein free insulin degrading enzyme with compound bdm41671 ((s)-2-{2-[carboxymethyl-(3-phenyl-propyl)-amino]-acetylamino}-3-(1h-imidazol-4-yl)-propionic acid methyl ester) 提交 2012-02-24 | Assembly 3 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
42–1019（978 aa）
片段：UNP RESIDUES 42-1019
链 B
42–1019（978 aa）
片段：UNP RESIDUES 42-1019
|
突变：C110L,E111Q,C171S,C178A,C257V,C414L,C573N,C590S,C789S,C812A,C819A,C904S,C966N,C974A 突变：C110L,E111Q,C171S,C178A,C257V,C414L,C573N,C590S,C789S,C812A,C819A,C904S,C966N,C974A | MGK methyl N-(carboxymethyl)-N-(3-phenylpropyl)glycyl-L-histidinate × 2 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；287 K；10-13% PEG MME 5000, 100 MM HEPES PH 7.0, 4-14% TACSIMATE, 10% DIOXANE, VAPOR DIFFUSION, HANGING DROP, temperature 287K
|
分辨率 3.00 Å R-free 0.227 |
| 4GS8 Structure analysis of cysteine free insulin degrading enzyme (ide) with compound bdm43079 [{[(s)-2-(1h-imidazol-4-yl)-1-methylcarbamoyl-ethylcarbamoyl]-methyl}-(3-phenyl-propyl)-amino]-acetic acid 提交 2012-08-27 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
42–1019（978 aa）
片段：UNP RESIDUES 42-1019
|
突变：C110L,E111Q,C171S,C178A,C257V,C414L,C573N,C590S,C789SC812A,C819A,C904S,C966N,C974A | MGJ N-(carboxymethyl)-N-(3-phenylpropyl)glycyl-N-methyl-L-histidinamide × 1 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；10-13% PEG MME 5000, 100 MM HEPES PH 7.0, 4-14% TACSIMATE, 10% DIOXANE, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 289K
|
分辨率 2.99 Å R-free 0.235 |
| 4GS8 Structure analysis of cysteine free insulin degrading enzyme (ide) with compound bdm43079 [{[(s)-2-(1h-imidazol-4-yl)-1-methylcarbamoyl-ethylcarbamoyl]-methyl}-(3-phenyl-propyl)-amino]-acetic acid 提交 2012-08-27 | Assembly 2 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
42–1019（978 aa）
片段：UNP RESIDUES 42-1019
|
突变：C110L,E111Q,C171S,C178A,C257V,C414L,C573N,C590S,C789SC812A,C819A,C904S,C966N,C974A | MGJ N-(carboxymethyl)-N-(3-phenylpropyl)glycyl-N-methyl-L-histidinamide × 1 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；10-13% PEG MME 5000, 100 MM HEPES PH 7.0, 4-14% TACSIMATE, 10% DIOXANE, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 289K
|
分辨率 2.99 Å R-free 0.235 |
| 4GSC Structure analysis of insulin degrading enzyme with compound bdm41559 ((s)-2-[2-(carboxymethyl-phenethyl-amino)-acetylamino]-3-(1h-imidazol-4-yl)-propionic acid methyl ester) 提交 2012-08-27 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
42–1019（978 aa）
片段：UNP RESIDUES 42-1019
|
突变：C110L,E111Q,C171S,C178A,C257V,C414L,C573N,C590S,C789S,C812A,C819A,C904S,C966N,C974A | ZN ZINC ION × 1 MGW methyl N-(carboxymethyl)-N-(2-phenylethyl)glycyl-L-histidinate × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；10-13% PEG MME 5000, 100 MM HEPES PH 7.0, 4-14% TACSIMATE, 10% DIOXANE, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 287K
|
分辨率 2.81 Å R-free 0.253 |
| 4GSC Structure analysis of insulin degrading enzyme with compound bdm41559 ((s)-2-[2-(carboxymethyl-phenethyl-amino)-acetylamino]-3-(1h-imidazol-4-yl)-propionic acid methyl ester) 提交 2012-08-27 | Assembly 2 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
42–1019（978 aa）
片段：UNP RESIDUES 42-1019
|
突变：C110L,E111Q,C171S,C178A,C257V,C414L,C573N,C590S,C789S,C812A,C819A,C904S,C966N,C974A | ZN ZINC ION × 1 MGW methyl N-(carboxymethyl)-N-(2-phenylethyl)glycyl-L-histidinate × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；10-13% PEG MME 5000, 100 MM HEPES PH 7.0, 4-14% TACSIMATE, 10% DIOXANE, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 287K
|
分辨率 2.81 Å R-free 0.253 |
| 4GSC Structure analysis of insulin degrading enzyme with compound bdm41559 ((s)-2-[2-(carboxymethyl-phenethyl-amino)-acetylamino]-3-(1h-imidazol-4-yl)-propionic acid methyl ester) 提交 2012-08-27 | Assembly 3 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
42–1019（978 aa）
片段：UNP RESIDUES 42-1019
链 B
42–1019（978 aa）
片段：UNP RESIDUES 42-1019
|
突变：C110L,E111Q,C171S,C178A,C257V,C414L,C573N,C590S,C789S,C812A,C819A,C904S,C966N,C974A 突变：C110L,E111Q,C171S,C178A,C257V,C414L,C573N,C590S,C789S,C812A,C819A,C904S,C966N,C974A | ZN ZINC ION × 2 MGW methyl N-(carboxymethyl)-N-(2-phenylethyl)glycyl-L-histidinate × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；10-13% PEG MME 5000, 100 MM HEPES PH 7.0, 4-14% TACSIMATE, 10% DIOXANE, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 287K
|
分辨率 2.81 Å R-free 0.253 |
| 4GSF The structure analysis of cysteine free insulin degrading enzyme (ide) with (s)-2-{2-[carboxymethyl-(3-phenyl-propionyl)-amino]-acetylamino}-3-(3h-imidazol-4-yl)-propionic acid methyl ester 提交 2012-08-27 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
42–1019（978 aa）
片段：UNP RESIDUES 42-1019
|
突变：C110L,E111Q,C171S,C178A,C257V,C414L,C573N,C590S,C789S,C812A,C819A,C904S,C966N,C974A | MGH methyl N-(carboxymethyl)-N-(3-phenylpropanoyl)glycyl-D-histidinate × 1 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；10-13% PEG MME 5000, 100 MM HEPES PH 7.0, 4-14% TACSIMATE, 10% DIOXANE, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 287K
|
分辨率 2.70 Å R-free 0.220 |
| 4GSF The structure analysis of cysteine free insulin degrading enzyme (ide) with (s)-2-{2-[carboxymethyl-(3-phenyl-propionyl)-amino]-acetylamino}-3-(3h-imidazol-4-yl)-propionic acid methyl ester 提交 2012-08-27 | Assembly 2 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
42–1019（978 aa）
片段：UNP RESIDUES 42-1019
|
突变：C110L,E111Q,C171S,C178A,C257V,C414L,C573N,C590S,C789S,C812A,C819A,C904S,C966N,C974A | MGH methyl N-(carboxymethyl)-N-(3-phenylpropanoyl)glycyl-D-histidinate × 1 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；10-13% PEG MME 5000, 100 MM HEPES PH 7.0, 4-14% TACSIMATE, 10% DIOXANE, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 287K
|
分辨率 2.70 Å R-free 0.220 |
| 4IFH Crystal structure of human insulin degrading enzyme (IDE) in complex with compound BDM44619 提交 2012-12-14 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
42–1019（978 aa）
链 B
42–1019（978 aa）
|
突变：C110L, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966N, C974A 突变：C110L, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966N, C974A | ZN ZINC ION × 2 1EF N-({1-[(2R)-4-(hydroxyamino)-1-(naphthalen-2-yl)-4-oxobutan-2-yl]-1H-1,2,3-triazol-4-yl}methyl)-4-methylbenzamide × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；291.15 K；10% PEG 5000, 100mM HEPES, 14% Tacsimate, 10% Dioxane, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K
|
分辨率 3.29 Å R-free 0.224 |
| 4IFH Crystal structure of human insulin degrading enzyme (IDE) in complex with compound BDM44619 提交 2012-12-14 | Assembly 2 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
42–1019（978 aa）
|
突变：C110L, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966N, C974A | ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；291.15 K；10% PEG 5000, 100mM HEPES, 14% Tacsimate, 10% Dioxane, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K
|
分辨率 3.29 Å R-free 0.224 |
| 4IFH Crystal structure of human insulin degrading enzyme (IDE) in complex with compound BDM44619 提交 2012-12-14 | Assembly 3 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
42–1019（978 aa）
|
突变：C110L, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966N, C974A | ZN ZINC ION × 1 1EF N-({1-[(2R)-4-(hydroxyamino)-1-(naphthalen-2-yl)-4-oxobutan-2-yl]-1H-1,2,3-triazol-4-yl}methyl)-4-methylbenzamide × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；291.15 K；10% PEG 5000, 100mM HEPES, 14% Tacsimate, 10% Dioxane, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K
|
分辨率 3.29 Å R-free 0.224 |
| 4IOF Crystal structure analysis of Fab-bound human Insulin Degrading Enzyme (IDE) 提交 2013-01-07 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 6 PDB 声明：hexameric（6） 与蛋白数一致 |
链 A
42–1019（978 aa）
链 B
42–1019（978 aa）
|
未记录 | ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.5；291.15 K；0.1M Sodium cacodylate, pH6.5; 0.2M MgCl2; 10% PEG3000, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K
|
分辨率 3.35 Å R-free 0.280 |
| 4LTE Structure of Cysteine-free Human Insulin Degrading Enzyme in Complex with Macrocyclic Inhibitor 提交 2013-07-23 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
42–1019（978 aa）
片段：UNP RESIDUES 42-1019
|
突变：C110L, E111Q, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966N, C974A | ZN ZINC ION × 1 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 LYN 2,6-DIAMINO-HEXANOIC ACID AMIDE × 1 FUM FUMARIC ACID × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；0.1 M HEPES, 20 % PEGMME-5000, 12 % tacsimate, 10 % dioxane, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
分辨率 2.71 Å R-free 0.200 |
| 4LTE Structure of Cysteine-free Human Insulin Degrading Enzyme in Complex with Macrocyclic Inhibitor 提交 2013-07-23 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 B
42–1019（978 aa）
片段：UNP RESIDUES 42-1019
|
突变：C110L, E111Q, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966N, C974A | ZN ZINC ION × 1 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 LYN 2,6-DIAMINO-HEXANOIC ACID AMIDE × 1 FUM FUMARIC ACID × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；0.1 M HEPES, 20 % PEGMME-5000, 12 % tacsimate, 10 % dioxane, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
分辨率 2.71 Å R-free 0.200 |
| 4M1C Crystal Structure Analysis of Fab-Bound Human Insulin Degrading Enzyme (IDE) in Complex with Amyloid-Beta (1-40) 提交 2013-08-02 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 A
42–1019（978 aa）
|
突变：E111Q | ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.5；291.15 K；0.1M Sodium cacodylate, pH6.5, 0.2M MgCl2, 10% PEG-3000, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K
|
分辨率 3.50 Å R-free 0.270 |
| 4M1C Crystal Structure Analysis of Fab-Bound Human Insulin Degrading Enzyme (IDE) in Complex with Amyloid-Beta (1-40) 提交 2013-08-02 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
42–1019（978 aa）
|
突变：E111Q | ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.5；291.15 K；0.1M Sodium cacodylate, pH6.5, 0.2M MgCl2, 10% PEG-3000, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K
|
分辨率 3.50 Å R-free 0.270 |
| 4NXO Crystal Structure of Insulin Degrading Enzyme in complex with BDM44768 提交 2013-12-09 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
42–1019（978 aa）
片段：UNP residues 42-1019
|
未记录 | ZN ZINC ION × 1 2H7 4-fluoro-N-({1-[(2R)-4-(hydroxyamino)-1-(naphthalen-2-yl)-4-oxobutan-2-yl]-1H-1,2,3-triazol-4-yl}methyl)benzamide × 1 1PE PENTAETHYLENE GLYCOL × 1 PEG DI(HYDROXYETHYL)ETHER × 3 EDO 1,2-ETHANEDIOL × 2 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 7；291.15 K；0.1M HEPES, pH 7.0, 10% Tacsimate, 10%Dioxane, 10% PEG-5000, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K
|
分辨率 2.73 Å R-free 0.219 |
| 4NXO Crystal Structure of Insulin Degrading Enzyme in complex with BDM44768 提交 2013-12-09 | Assembly 2 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
42–1019（978 aa）
片段：UNP residues 42-1019
|
未记录 | ZN ZINC ION × 1 2H7 4-fluoro-N-({1-[(2R)-4-(hydroxyamino)-1-(naphthalen-2-yl)-4-oxobutan-2-yl]-1H-1,2,3-triazol-4-yl}methyl)benzamide × 1 1PE PENTAETHYLENE GLYCOL × 1 PEG DI(HYDROXYETHYL)ETHER × 4 EDO 1,2-ETHANEDIOL × 2 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 DIO 1,4-DIETHYLENE DIOXIDE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 7；291.15 K；0.1M HEPES, pH 7.0, 10% Tacsimate, 10%Dioxane, 10% PEG-5000, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K
|
分辨率 2.73 Å R-free 0.219 |
| 4PES Crystal structure of insulin degrading enzyme complexed with inhibitor tert-butyl [(2S)-2-(2,5-difluorophenyl)-3-(quinolin-3-yl)propyl]carbamate 提交 2014-04-24 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
42–1019（978 aa）
片段：UNP residues 42-1019
|
突变：C110L, E111Q, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966N, C974A | 2PJ tert-butyl [(2S)-2-(2,5-difluorophenyl)-3-(quinolin-3-yl)propyl]carbamate × 1 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；298 K；20% PEG3350, 0.2 mM sodium thiocyanate
|
分辨率 2.21 Å R-free 0.256 |
| 4PES Crystal structure of insulin degrading enzyme complexed with inhibitor tert-butyl [(2S)-2-(2,5-difluorophenyl)-3-(quinolin-3-yl)propyl]carbamate 提交 2014-04-24 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 B
42–1019（978 aa）
片段：UNP residues 42-1019
|
突变：C110L, E111Q, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966N, C974A | 2PJ tert-butyl [(2S)-2-(2,5-difluorophenyl)-3-(quinolin-3-yl)propyl]carbamate × 1 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；298 K；20% PEG3350, 0.2 mM sodium thiocyanate
|
分辨率 2.21 Å R-free 0.256 |
| 4PF7 Crystal structure of insulin degrading enzyme complexed with inhibitor 提交 2014-04-28 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
42–1019（978 aa）
片段：UNP residues 42-1019
链 B
42–1019（978 aa）
片段：UNP residues 42-1019
|
突变：C110L, E111Q, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966N, C974A 突变：C110L, E111Q, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966N, C974A | 2QW (2S)-2-amino-N-{(1S)-1-cyclohexyl-2-[(4-methylphenyl)amino]-2-oxoethyl}-4-(methylselanyl)butanamide × 2 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；298 K；20% PEG3350, 0.2 mM sodium thiocyanate
|
分辨率 2.33 Å R-free 0.236 |
| 4PF9 Crystal structure of insulin degrading enzyme complexed with inhibitor 提交 2014-04-28 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
42–1019（978 aa）
片段：UNP residues 42-1019
链 B
42–1019（978 aa）
片段：UNP residues 42-1019
|
突变：C110L, E111Q, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966N, C974A 突变：C110L, E111Q, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966N, C974A | 2Q6 methyl [(2S)-2-[4-({5-[4-({(2S)-2-[(3S)-3-amino-2-oxopiperidin-1-yl]-2-cyclohexylacetyl}amino)phenyl]pentyl}oxy)phenyl]-3-(quinolin-3-yl)propyl]carbamate × 2 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；298 K；20% PEG3350, 0.2 mM sodium thiocyanate
|
分辨率 2.50 Å R-free 0.253 |
| 4PFC Crystal structure of insulin degrading enzyme complexed with inhibitor 提交 2014-04-28 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
42–1019（978 aa）
片段：UNP residues 42-1019
链 B
42–1019（978 aa）
片段：UNP residues 42-1019
|
突变：C110L, E111Q, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966N, C974A 突变：C110L, E111Q, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966N, C974A | 2QX methyl [(2S)-2-(5-{5-[4-({(2S)-2-[(3S)-3-amino-2-oxopiperidin-1-yl]-2-cyclohexylacetyl}amino)phenyl]pentyl}-2-fluorophenyl)-3-(quinolin-3-yl)propyl]carbamate × 2 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；298 K；20% PEG3350, 0.2 mM sodium thiocyanate
|
分辨率 2.21 Å R-free 0.233 |
| 4QIA Crystal structure of human insulin degrading enzyme (ide) in complex with inhibitor N-benzyl-N-(carboxymethyl)glycyl-L-histidine 提交 2014-05-30 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
42–1019（978 aa）
|
未记录 | 33K N-benzyl-N-(carboxymethyl)glycyl-L-histidine × 1 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；287 K；10-13% PEG MME 5000, 100 MM HEPES PH 7.0, 4-14% TACSIMATE, 10% DIOXANE, VAPOR DIFFUSION, HANGING DROP, temperature 287K
|
分辨率 3.20 Å R-free 0.222 |
| 4QIA Crystal structure of human insulin degrading enzyme (ide) in complex with inhibitor N-benzyl-N-(carboxymethyl)glycyl-L-histidine 提交 2014-05-30 | Assembly 2 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
42–1019（978 aa）
|
未记录 | 33K N-benzyl-N-(carboxymethyl)glycyl-L-histidine × 1 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；287 K；10-13% PEG MME 5000, 100 MM HEPES PH 7.0, 4-14% TACSIMATE, 10% DIOXANE, VAPOR DIFFUSION, HANGING DROP, temperature 287K
|
分辨率 3.20 Å R-free 0.222 |
| 4RAL Crystal structure of insulin degrading enzyme in complex with macrophage inflammatory protein 1 beta 提交 2014-09-10 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
42–1019（978 aa）
片段：UNP residues 42-1019
|
突变：C110L, E111Q, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966N, C974A | ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；291 K；13% PEG5000 MME, 100 mM HEPES, pH 7.0, 10% Tacsimate, 10% dioxane, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K
|
分辨率 3.15 Å R-free 0.255 |
| 4RAL Crystal structure of insulin degrading enzyme in complex with macrophage inflammatory protein 1 beta 提交 2014-09-10 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 B
42–1019（978 aa）
片段：UNP residues 42-1019
|
突变：C110L, E111Q, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966N, C974A | ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；291 K；13% PEG5000 MME, 100 mM HEPES, pH 7.0, 10% Tacsimate, 10% dioxane, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K
|
分辨率 3.15 Å R-free 0.255 |
| 4RE9 Crystal structure of human insulin degrading enzyme (IDE) in complex with compound 71290 提交 2014-09-22 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
42–1019（978 aa）
片段：UNP residues 42-1019
|
突变：C110L, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966N, C974A | ZN ZINC ION × 1 3M9 4-fluoro-N-({1-[(2R)-4-(hydroxyamino)-1-(naphthalen-2-yl)-4-oxobutan-2-yl]-1H-1,2,3-triazol-5-yl}methyl)benzamide × 1 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 PEG DI(HYDROXYETHYL)ETHER × 6 1PE PENTAETHYLENE GLYCOL × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；291.15 K；10% PEG5000, 100 mM HEPES, 14% Tacsimate, 10% dioxane, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K
|
分辨率 2.91 Å R-free 0.213 |
| 4RE9 Crystal structure of human insulin degrading enzyme (IDE) in complex with compound 71290 提交 2014-09-22 | Assembly 2 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
42–1019（978 aa）
片段：UNP residues 42-1019
|
突变：C110L, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966N, C974A | ZN ZINC ION × 1 3M9 4-fluoro-N-({1-[(2R)-4-(hydroxyamino)-1-(naphthalen-2-yl)-4-oxobutan-2-yl]-1H-1,2,3-triazol-5-yl}methyl)benzamide × 1 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 PEG DI(HYDROXYETHYL)ETHER × 4 1PE PENTAETHYLENE GLYCOL × 1 EDO 1,2-ETHANEDIOL × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；291.15 K；10% PEG5000, 100 mM HEPES, 14% Tacsimate, 10% dioxane, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K
|
分辨率 2.91 Å R-free 0.213 |
| 5CJO Crystal Structure Analysis of Elbow-Engineered-Fab-Bound Human Insulin Degrading Enzyme (IDE) in Complex with Insulin 提交 2015-07-14 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 8 PDB 声明：octameric（8） 与蛋白数一致 |
链 A
42–1019（978 aa）
|
突变：C110L, E111Q, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966N, C974A | EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 2 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；291.15 K；6% v/v Tacsimate pH 7.0, 0.1 M HEPES pH 7.0, 8% w/v Polyethylene glycol monomethyl ether 5,000, and 8% v/v tert-butanol as additive.
|
分辨率 3.29 Å R-free 0.245 |
| 5UOE Crystal Structure Analysis of Elbow-Engineered-Fab-Bound Human Insulin Degrading Enzyme (IDE) 提交 2017-01-31 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 3 PDB 声明：trimeric（3） 与蛋白数一致 |
链 A
42–1019（978 aa）
|
突变：C110L, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966N, C974A. | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；291.15 K；0.088M Ammonium citrate tribasic, ph 7, 10% w/v peg3350, 0.02 M Ethylenediaminetetraacetic disodium salt dihydrate
|
分辨率 3.80 Å R-free 0.270 |
| 5UOE Crystal Structure Analysis of Elbow-Engineered-Fab-Bound Human Insulin Degrading Enzyme (IDE) 提交 2017-01-31 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 3 PDB 声明：trimeric（3） 与蛋白数一致 |
链 B
42–1019（978 aa）
|
突变：C110L, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966N, C974A. | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；291.15 K；0.088M Ammonium citrate tribasic, ph 7, 10% w/v peg3350, 0.02 M Ethylenediaminetetraacetic disodium salt dihydrate
|
分辨率 3.80 Å R-free 0.270 |
| 5UOE Crystal Structure Analysis of Elbow-Engineered-Fab-Bound Human Insulin Degrading Enzyme (IDE) 提交 2017-01-31 | Assembly 3 蛋白异源复合物 异源复合物；蛋白 × 3 PDB 声明：trimeric（3） 与蛋白数一致 |
链 C
42–1019（978 aa）
|
突变：C110L, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966N, C974A. | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；291.15 K；0.088M Ammonium citrate tribasic, ph 7, 10% w/v peg3350, 0.02 M Ethylenediaminetetraacetic disodium salt dihydrate
|
分辨率 3.80 Å R-free 0.270 |
| 5UOE Crystal Structure Analysis of Elbow-Engineered-Fab-Bound Human Insulin Degrading Enzyme (IDE) 提交 2017-01-31 | Assembly 4 蛋白异源复合物 异源复合物；蛋白 × 3 PDB 声明：trimeric（3） 与蛋白数一致 |
链 D
42–1019（978 aa）
|
突变：C110L, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966N, C974A. | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；291.15 K；0.088M Ammonium citrate tribasic, ph 7, 10% w/v peg3350, 0.02 M Ethylenediaminetetraacetic disodium salt dihydrate
|
分辨率 3.80 Å R-free 0.270 |
| 5UOE Crystal Structure Analysis of Elbow-Engineered-Fab-Bound Human Insulin Degrading Enzyme (IDE) 提交 2017-01-31 | Assembly 5 蛋白异源复合物 异源复合物；蛋白 × 3 PDB 声明：trimeric（3） 与蛋白数一致 |
链 E
42–1019（978 aa）
|
突变：C110L, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966N, C974A. | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；291.15 K；0.088M Ammonium citrate tribasic, ph 7, 10% w/v peg3350, 0.02 M Ethylenediaminetetraacetic disodium salt dihydrate
|
分辨率 3.80 Å R-free 0.270 |
| 5WOB Crystal Structure Analysis of Fab1-Bound Human Insulin Degrading Enzyme (IDE) in Complex with Insulin 提交 2017-08-01 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 8 PDB 声明：octameric（8） 与蛋白数一致 |
链 A
42–1019（978 aa）
片段：UNP residues 42-1019
链 B
42–1019（978 aa）
片段：UNP residues 42-1019
|
未记录 | ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.5；291.15 K；0.1M Sodium cacodylate, pH6.5; 0.2M MgCl2; 10% PEG3000, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K
|
分辨率 3.95 Å R-free 0.291 |
| 5WOB Crystal Structure Analysis of Fab1-Bound Human Insulin Degrading Enzyme (IDE) in Complex with Insulin 提交 2017-08-01 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 8 PDB 声明：octameric（8） 与蛋白数一致 |
链 C
42–1019（978 aa）
片段：UNP residues 42-1019
链 D
42–1019（978 aa）
片段：UNP residues 42-1019
|
未记录 | ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.5；291.15 K；0.1M Sodium cacodylate, pH6.5; 0.2M MgCl2; 10% PEG3000, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K
|
分辨率 3.95 Å R-free 0.291 |
| 5WOB Crystal Structure Analysis of Fab1-Bound Human Insulin Degrading Enzyme (IDE) in Complex with Insulin 提交 2017-08-01 | Assembly 3 蛋白异源复合物 异源复合物；蛋白 × 8 PDB 声明：octameric（8） 与蛋白数一致 |
链 E
42–1019（978 aa）
片段：UNP residues 42-1019
链 F
42–1019（978 aa）
片段：UNP residues 42-1019
|
未记录 | ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.5；291.15 K；0.1M Sodium cacodylate, pH6.5; 0.2M MgCl2; 10% PEG3000, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K
|
分辨率 3.95 Å R-free 0.291 |
| 5WOB Crystal Structure Analysis of Fab1-Bound Human Insulin Degrading Enzyme (IDE) in Complex with Insulin 提交 2017-08-01 | Assembly 4 蛋白异源复合物 异源复合物；蛋白 × 8 PDB 声明：octameric（8） 与蛋白数一致 |
链 G
42–1019（978 aa）
片段：UNP residues 42-1019
链 H
42–1019（978 aa）
片段：UNP residues 42-1019
|
未记录 | ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.5；291.15 K；0.1M Sodium cacodylate, pH6.5; 0.2M MgCl2; 10% PEG3000, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K
|
分辨率 3.95 Å R-free 0.291 |
| 6B3Q Cryo-EM structure of human insulin degrading enzyme in complex with insulin 提交 2017-09-22 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 A
42–1019（978 aa）
片段：residues 42-1019
链 B
42–1019（978 aa）
片段：residues 42-1019
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.8
cryo-EM玻璃化条件
冷冻剂 ETHANE；The cryo grids were made using Spotiton and homemade plunger
|
分辨率 3.70 Å |
| 6B70 Cryo-EM structure of human insulin degrading enzyme in complex with FAB H11-E heavy chain, FAB H11-E light chain and insulin 提交 2017-10-03 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 8 PDB 声明：octameric（8） 与蛋白数一致 |
链 A
46–1011（966 aa）
片段：residues 46-1011
链 B
46–1011（966 aa）
片段：residues 46-1011
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.8
cryo-EM玻璃化条件
冷冻剂 ETHANE；The cryo grids were made using Spotiton and homemade plunger
|
分辨率 3.70 Å |
| 6B7Y Cryo-EM structure of human insulin degrading enzyme 提交 2017-10-05 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
46–1011（966 aa）
片段：residues 46-1011
链 B
46–1011（966 aa）
片段：residues 46-1011
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.8
cryo-EM玻璃化条件
冷冻剂 ETHANE；The cryo grids were made using Spotiton
|
分辨率 6.50 Å |
| 6B7Z Cryo-EM structure of human insulin degrading enzyme in complex with FAB H11 heavy chain and FAB H11 light chain 提交 2017-10-05 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 6 PDB 声明：hexameric（6） 与蛋白数一致 |
链 A
46–1011（966 aa）
片段：residues 46-1011
链 B
46–1011（966 aa）
片段：residues 46-1011
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.8
cryo-EM玻璃化条件
冷冻剂 ETHANE；The cryo grids were made using Spotiton
|
分辨率 6.50 Å |
| 6BF6 Cryo-EM structure of human insulin degrading enzyme 提交 2017-10-26 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
46–1011（966 aa）
片段：residues 46-1011
链 B
46–1011（966 aa）
片段：residues 46-1011
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.8
cryo-EM玻璃化条件
冷冻剂 ETHANE；The cryo grids were made using Spotiton
|
分辨率 6.50 Å |
| 6BF7 Cryo-EM structure of human insulin degrading enzyme in complex with FAB H11-E heavy chain, FAB H11-E light chain 提交 2017-10-26 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 6 PDB 声明：hexameric（6） 与蛋白数一致 |
链 A
46–1011（966 aa）
片段：residues 46-1011
链 B
46–1011（966 aa）
片段：residues 46-1011
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.8
cryo-EM玻璃化条件
冷冻剂 ETHANE；The cryo grids were made using Spotiton
|
分辨率 6.50 Å |
| 6BF8 Cryo-EM structure of human insulin degrading enzyme in complex with insulin 提交 2017-10-26 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
46–1011（966 aa）
片段：residues 46-1011
链 B
46–1011（966 aa）
片段：residues 46-1011
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.8
cryo-EM玻璃化条件
冷冻剂 ETHANE；The cryo grids were made using Spotiton
|
分辨率 4.20 Å |
| 6BF9 Cryo-EM structure of human insulin degrading enzyme in complex with FAB H11-E heavy chain, FAB H11-E light chain 提交 2017-10-26 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 6 PDB 声明：hexameric（6） 与蛋白数一致 |
链 A
46–1011（966 aa）
片段：residues 46-1011
链 B
46–1011（966 aa）
片段：residues 46-1011
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.8
cryo-EM玻璃化条件
冷冻剂 ETHANE；The cryo grids were made using Spotiton
|
分辨率 7.20 Å |
| 6BFC Cryo-EM structure of human insulin degrading enzyme in complex with insulin 提交 2017-10-26 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 A
46–1011（966 aa）
链 B
46–1011（966 aa）
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.8
cryo-EM玻璃化条件
冷冻剂 ETHANE；Grids made using Spotiton
|
分辨率 3.70 Å |
| 6BYZ Structure of Cysteine-free Human Insulin-Degrading Enzyme in complex with Substrate-selective Macrocyclic Inhibitor 37 提交 2017-12-21 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
1–1019（1019 aa）
|
突变：C110L, E111Q, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966N, C974A | J18 [(8R,9S,10S)-9-(2',3'-dimethyl[1,1'-biphenyl]-4-yl)-6-{[2-(trifluoromethyl)phenyl]sulfonyl}-1,6-diazabicyclo[6.2.0]decan-10-yl]methanol × 1 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.8；298 K；0.1 M HEPES, 12% Tacsimate, 20% PEGMME-5000, 10% 1,4-dioxane
|
分辨率 2.96 Å R-free 0.203 |
| 6BYZ Structure of Cysteine-free Human Insulin-Degrading Enzyme in complex with Substrate-selective Macrocyclic Inhibitor 37 提交 2017-12-21 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 B
1–1019（1019 aa）
|
突变：C110L, E111Q, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966N, C974A | J18 [(8R,9S,10S)-9-(2',3'-dimethyl[1,1'-biphenyl]-4-yl)-6-{[2-(trifluoromethyl)phenyl]sulfonyl}-1,6-diazabicyclo[6.2.0]decan-10-yl]methanol × 1 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.8；298 K；0.1 M HEPES, 12% Tacsimate, 20% PEGMME-5000, 10% 1,4-dioxane
|
分辨率 2.96 Å R-free 0.203 |
| 6EDS Structure of Cysteine-free Human Insulin-Degrading Enzyme in complex with Glucagon and Substrate-selective Macrocyclic Inhibitor 63 提交 2018-08-10 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
42–1019（978 aa）
|
突变：C110L, E111Q, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966N, C974A | J22 {(8R,9S,10S)-9-(2',3'-dimethyl[1,1'-biphenyl]-4-yl)-6-[(1-methyl-1H-imidazol-2-yl)sulfonyl]-1,6-diazabicyclo[6.2.0]decan-10-yl}methanol × 1 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 PEG DI(HYDROXYETHYL)ETHER × 1 DIO 1,4-DIETHYLENE DIOXIDE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；298 K；0.1M HEPES pH 7.0, 12% Tacsimate pH 7.0, 13% PEGMME, 10% Dioxane
|
分辨率 3.18 Å R-free 0.222 |
| 6EDS Structure of Cysteine-free Human Insulin-Degrading Enzyme in complex with Glucagon and Substrate-selective Macrocyclic Inhibitor 63 提交 2018-08-10 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 B
42–1019（978 aa）
|
突变：C110L, E111Q, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966N, C974A | J22 {(8R,9S,10S)-9-(2',3'-dimethyl[1,1'-biphenyl]-4-yl)-6-[(1-methyl-1H-imidazol-2-yl)sulfonyl]-1,6-diazabicyclo[6.2.0]decan-10-yl}methanol × 1 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 PEG DI(HYDROXYETHYL)ETHER × 1 DIO 1,4-DIETHYLENE DIOXIDE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；298 K；0.1M HEPES pH 7.0, 12% Tacsimate pH 7.0, 13% PEGMME, 10% Dioxane
|
分辨率 3.18 Å R-free 0.222 |
| 6MQ3 Structure of Cysteine-free Human Insulin-Degrading Enzyme in complex with Substrate-selective Macrocycle Inhibitor 63 提交 2018-10-09 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：monomeric（1） 待复核 |
链 A
42–1019（978 aa）
链 B
42–1019（978 aa）
|
突变：C110L, E111Q, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966N, C974A 突变：C110L, E111Q, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966N, C974A | J22 {(8R,9S,10S)-9-(2',3'-dimethyl[1,1'-biphenyl]-4-yl)-6-[(1-methyl-1H-imidazol-2-yl)sulfonyl]-1,6-diazabicyclo[6.2.0]decan-10-yl}methanol × 2 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；298 K；0.1M HEPES pH 6.5, 5% Tacsimate pH 7, 10% 1,4-Dioxane, 14% PEGGMME 5000
|
分辨率 3.57 Å R-free 0.211 |
| 6MQ3 Structure of Cysteine-free Human Insulin-Degrading Enzyme in complex with Substrate-selective Macrocycle Inhibitor 63 提交 2018-10-09 | Assembly 2 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：monomeric（1） 待复核 |
链 A
42–1019（978 aa）
链 B
42–1019（978 aa）
|
突变：C110L, E111Q, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966N, C974A 突变：C110L, E111Q, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966N, C974A | J22 {(8R,9S,10S)-9-(2',3'-dimethyl[1,1'-biphenyl]-4-yl)-6-[(1-methyl-1H-imidazol-2-yl)sulfonyl]-1,6-diazabicyclo[6.2.0]decan-10-yl}methanol × 2 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；298 K；0.1M HEPES pH 6.5, 5% Tacsimate pH 7, 10% 1,4-Dioxane, 14% PEGGMME 5000
|
分辨率 3.57 Å R-free 0.211 |
| 7K1D Crystal structure of human insulin degrading enzyme (IDE) in complex with compound BDM_77291 提交 2020-09-07 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
42–1019（978 aa）
链 B
42–1019（978 aa）
|
未记录 | ZN ZINC ION × 2 VQD (3R)-3-{4-[(3R)-4-(3,4-difluorobenzene-1-carbonyl)morpholin-3-yl]-1H-1,2,3-triazol-1-yl}-N-hydroxy-4-(naphthalen-2-yl)butanamide × 2 DIO 1,4-DIETHYLENE DIOXIDE × 6 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；291.15 K；10% PEG 5000, 100mM HEPES, 14% Tacsimate, 10% Dioxane, pH 7.0
|
分辨率 3.00 Å R-free 0.192 |
| 7K1E Crystal structure of human insulin degrading enzyme (IDE) in complex with compound BDM_88646 提交 2020-09-07 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
42–1019（978 aa）
链 B
42–1019（978 aa）
|
未记录 | ZN ZINC ION × 2 VQG 3,4-difluoro-N-[(1S)-1-{1-[(2R)-4-(hydroxyamino)-4-oxo-1-(5,6,7,8-tetrahydronaphthalen-2-yl)butan-2-yl]-1H-1,2,3-triazol-4-yl}ethyl]benzamide × 2 DIO 1,4-DIETHYLENE DIOXIDE × 6 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；291.15 K；10% PEG 5000, 100mM HEPES, 14% Tacsimate, 10% Dioxane, pH 7.0
|
分辨率 2.80 Å R-free 0.209 |
| 7K1F Crystal structure of human insulin degrading enzyme (IDE) in complex with compound BDM_88558 提交 2020-09-07 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
42–1019（978 aa）
链 B
42–1019（978 aa）
|
未记录 | ZN ZINC ION × 2 VQJ 3,4-difluoro-N-({1-[(2R)-4-(hydroxyamino)-4-oxo-1-(quinolin-7-yl)butan-2-yl]-1H-1,2,3-triazol-4-yl}methyl)benzamide × 2 DIO 1,4-DIETHYLENE DIOXIDE × 6 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；291.15 K；10% PEG 5000, 100mM HEPES, 14% Tacsimate, 10% Dioxane, pH 7.0
|
分辨率 2.60 Å R-free 0.208 |
| 7RZE Insulin Degrading Enzyme pO/pC 提交 2021-08-27 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 A
1–1011（1011 aa）
链 B
1–1011（1011 aa）
|
突变：C110L, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966S, C974S 突变：C110L, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966S, C974S | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.2
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.30 Å |
| 7RZF Insulin Degrading Enzyme O/pC 提交 2021-08-27 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 A
1–1011（1011 aa）
链 B
1–1011（1011 aa）
|
突变：C110L, C171S, C178A, 257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966S, C974S 突变：C110L, C171S, C178A, 257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966S, C974S | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.2
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.40 Å |
| 7RZG Insulin Degrading Enzyme O/pO 提交 2021-08-27 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
1–1011（1011 aa）
链 B
1–1011（1011 aa）
|
突变：C110L, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966S, C974S 突变：C110L, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966S, C974S | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.2
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 4.10 Å |
| 7RZH Insulin Degrading Enzyme O/O 提交 2021-08-27 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
1–1011（1011 aa）
链 B
1–1011（1011 aa）
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.2
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.80 Å |
| 7RZI Insulin Degrading Enzyme pC/pC 提交 2021-08-27 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 6 PDB 声明：hexameric（6） 与蛋白数一致 |
链 A
1–1011（1011 aa）
链 B
1–1011（1011 aa）
|
突变：C110L, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966S, C974S 突变：C110L, C171S, C178A, C257V, C414L, C573N, C590S, C789S, C812A, C819A, C904S, C966S, C974S | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.2
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.00 Å |
| 9Y0H Insulin Degrading Enzyme Time-resolved O/O state 提交 2025-08-28 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
1–1011（1011 aa）
链 B
1–1011（1011 aa）
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 5.10 Å |