Current Protein Identity:P14736
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 2M14 NMR structure of the complex between the PH domain of the Tfb1 subunit from TFIIH and Rad4 Deposited 2012-11-16 | Assembly 1 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain B
76–115(40 aa)
Fragment:UNP residues 76-115
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 6.5;300 K;Ionic strength (raw mmCIF value) 20;Pressure ambient
NMR sample composition
1 mM [U-100% 13C; U-100% 15N] Tfb1, 1.25 mM Rad4, 20 mM sodium phosphate, 1 mM EDTA, 1 mM DTT, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
1 mM [U-100% 13C; U-100% 15N] Tfb1, 1.25 mM Rad4, 20 mM sodium phosphate, 1 mM EDTA, 1 mM DTT, 100% D2O | 100% D2O
NMR sample composition
1 mM [U-100% 15N] Tfb1, 1.25 mM Rad4, 20 mM sodium phosphate, 1 mM EDTA, 1 mM DTT, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
1.25 mM Tfb1, 1 mM [U-100% 13C; U-100% 15N] Rad4, 20 mM sodium phosphate, 1 mM EDTA, 1 mM DTT, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
1.25 mM Tfb1, 1 mM [U-100% 13C; U-100% 15N] Rad4, 20 mM sodium phosphate, 1 mM EDTA, 1 mM DTT, 100% D2O | 100% D2O
NMR sample composition
1.25 mM Tfb1, 1 mM [U-100% 15N] Rad4, 20 mM sodium phosphate, 1 mM EDTA, 1 mM DTT, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 2QSF Crystal structure of the Rad4-Rad23 complex Deposited 2007-07-31 | Assembly 1 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain A
101–632(532 aa)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6.5;277 K;100 mM sodium phosphate, 8% (w/v) PEG 2000, 2 mM dithiothreitol, pH 6.5, hanging-drop vapor diffusion, temperature 277K, VAPOR DIFFUSION, HANGING DROP
|
Resolution 2.35 Å R-free 0.245 |
| 2QSG Crystal structure of Rad4-Rad23 bound to a UV-damaged DNA Deposited 2007-07-31 | Assembly 1 Protein–DNA Heteromer;Protein × 2 PDB declaration: tetrameric(4) Consistent with all polymers |
Chain A
101–632(532 aa)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6.8;277 K;50 mM bis-tris propane, 100mM sodium chloride, 6% (v/v) isopropanol, 14 mM calcium chloride and 5 mM
dithiothreitol, pH 6.8, hanging-drop vapor diffusion, temperature 277K, VAPOR DIFFUSION, HANGING DROP
|
Resolution 3.10 Å R-free 0.277 |
| 2QSH Crystal structure of Rad4-Rad23 bound to a mismatch DNA Deposited 2007-07-31 | Assembly 1 Protein–DNA Heteromer;Protein × 2 PDB declaration: tetrameric(4) Consistent with all polymers |
Chain A
101–632(532 aa)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6.8;277 K;50 mM bis-tris propane, 100 mM sodium chloride, 15% (v/v) isopropanol, 10 mM calcium chloride and 5 mM dithiothreitol, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.81 Å R-free 0.244 |
| 4YIR Crystal structure of Rad4-Rad23 crosslinked to an undamaged DNA Deposited 2015-03-02 | Assembly 1 Protein–DNA Heteromer;Protein × 2 PDB declaration: tetrameric(4) Consistent with all polymers |
Chain A
101–632(532 aa)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;277 K;50mM BTP-HCl, 100mM NaCl, 14%
isopropanol and 100mM calcium chloride
|
Resolution 3.05 Å R-free 0.253 |
| 6CFI Crystal structure of Rad4-Rad23 bound to a 6-4 photoproduct UV lesion Deposited 2018-02-15 | Assembly 1 Protein–DNA Heteromer;Protein × 2 PDB declaration: tetrameric(4) Consistent with all polymers |
Chain A
101–632(532 aa)
Fragment:residues 101-632
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6.8;277 K;bis-tris propane 5 mM
sodium chloride 100 mM
1-propanol 14%
spermidine-HCl 5 mM
dithiothreitol 5 mM
|
Resolution 3.36 Å R-free 0.268 |
| 6UBF Role of Beta-hairpin motifs in the DNA duplex opening by the Rad4/XPC nucleotide excision repair complex Deposited 2019-09-11 | Assembly 1 Protein–DNA Heteromer;Protein × 2 PDB declaration: tetrameric(4) Consistent with all polymers |
Chain A
101–632(532 aa)
Fragment:UNP residues 101-632
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6.8;277 K;50 mM BTP-HCl, 150 mM sodium chloride, 12% isopropanol
|
Resolution 4.60 Å R-free 0.356 |
| 6UG1 Sequence impact in DNA duplex opening by the Rad4/XPC nucleotide excision repair complex Deposited 2019-09-25 | Assembly 1 Protein–DNA Heteromer;Protein × 2 PDB declaration: tetrameric(4) Consistent with all polymers |
Chain A
129–632(504 aa)
Fragment:UNP residues 129-632
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6.8;277 K;50 mM BTP-HCl, 150 mM sodium chloride, 12% isopropanol, 100 mM calcium chloride
|
Resolution 2.83 Å R-free 0.272 |
| 6UIN Role of Beta-hairpin motifs in the DNA duplex opening by the Rad4/XPC nucleotide excision repair complex Deposited 2019-10-01 | Assembly 1 Protein–DNA Heteromer;Protein × 2 PDB declaration: tetrameric(4) Consistent with all polymers |
Chain A
101–632(532 aa)
Fragment:UNP residues 101-632
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6.8;277 K;50 mM BTP-HCl, 200 mM sodium chloride, 12% isopropanol, 100 mM calcium chloride
|
Resolution 3.35 Å R-free 0.262 |
| 7K04 Structure of TFIIH/Rad4-Rad23-Rad33/DNA in DNA opening Deposited 2020-09-03 | Assembly 1 Protein–DNA Heteromer;Protein × 9 PDB declaration: undecameric(11) Consistent with all polymers |
Chain A
1–754(754 aa)
|
Not recorded | CA CALCIUM ION × 2 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE;Manually blotted by Leica EM CPC
|
Resolution 9.25 Å |
| 7M2U Nucleotide Excision Repair complex TFIIH Rad4-33 Deposited 2021-03-17 | Assembly 1 Protein–DNA Heteromer;Protein × 9 PDB declaration: undecameric(11) Consistent with all polymers |
Chain A
1–754(754 aa)
|
Not recorded | SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 5 CA CALCIUM ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 8.20 Å |