当前蛋白身份：P18356
重新检索
差异标签只比较当前检索结果；所有PDB和assembly原始记录仍分别保留。
相关结构差异明细
一行代表一个 PDB 条目中的一个 biological assembly；同一蛋白的多个单体会分别列出。
| PDB 条目 | Assembly / 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与非聚合物 | 实验方法 | 实验环境 | 结构质量 |
|---|---|---|---|---|---|---|---|
| 2JSF Solution structures of the envelope protein domain III from the dengue-2 virus 提交 2007-07-03 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
469–577（109 aa）
片段：Residues 469-577
|
未记录 | 未记录非水小分子 | SOLUTION NMR |
NMR测量条件
pH 7.4；298 K；离子强度（mmCIF原始值）0.31；压力 ambient
NMR样品组成
1-5 mM EDTA, 1-5 mM DTT, 1 mM sodium azide, 90% H2O/10% D2O | 90% H2O/10% D2O
|
分辨率未提供 |
| 2R69 Crystal structure of Fab 1A1D-2 complexed with E-DIII of Dengue virus at 3.8 angstrom resolution 提交 2007-09-05 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 3 PDB 声明：trimeric（3） 与蛋白数一致 |
链 A
478–574（97 aa）
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 5.8；298 K；12% PEG 3350, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
分辨率 3.80 Å R-free 0.363 |
| 2R6P Fit of E protein and Fab 1A1D-2 into 24 angstrom resolution cryoEM map of Fab complexed with dengue 2 virus. 提交 2007-09-06 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 420 PDB 声明：420-MERIC（420） 与蛋白数一致 |
链 A
181–570（390 aa）
片段：E protein
链 B
181–570（390 aa）
片段：E protein
链 C
181–570（390 aa）
片段：E protein
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.6
cryo-EM玻璃化条件
冷冻剂 ETHANE；SAMPLES WERE PREPARED AS
THIN LAYERS OF VITREOUS ICE
AND MAINTAINED AT LIQUID
NITROGEN TEMPERATURE IN THE
ELECTRON MICROSCOPE
|
分辨率 24.00 Å |
| 2R6P Fit of E protein and Fab 1A1D-2 into 24 angstrom resolution cryoEM map of Fab complexed with dengue 2 virus. 提交 2007-09-06 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 7 PDB 声明：heptameric（7） 与蛋白数一致 |
链 A
181–570（390 aa）
片段：E protein
链 B
181–570（390 aa）
片段：E protein
链 C
181–570（390 aa）
片段：E protein
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.6
cryo-EM玻璃化条件
冷冻剂 ETHANE；SAMPLES WERE PREPARED AS
THIN LAYERS OF VITREOUS ICE
AND MAINTAINED AT LIQUID
NITROGEN TEMPERATURE IN THE
ELECTRON MICROSCOPE
|
分辨率 24.00 Å |
| 2R6P Fit of E protein and Fab 1A1D-2 into 24 angstrom resolution cryoEM map of Fab complexed with dengue 2 virus. 提交 2007-09-06 | Assembly 3 蛋白异源复合物 异源复合物；蛋白 × 35 PDB 声明：35-meric（35） 与蛋白数一致 |
链 A
181–570（390 aa）
片段：E protein
链 B
181–570（390 aa）
片段：E protein
链 C
181–570（390 aa）
片段：E protein
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.6
cryo-EM玻璃化条件
冷冻剂 ETHANE；SAMPLES WERE PREPARED AS
THIN LAYERS OF VITREOUS ICE
AND MAINTAINED AT LIQUID
NITROGEN TEMPERATURE IN THE
ELECTRON MICROSCOPE
|
分辨率 24.00 Å |
| 2R6P Fit of E protein and Fab 1A1D-2 into 24 angstrom resolution cryoEM map of Fab complexed with dengue 2 virus. 提交 2007-09-06 | Assembly 4 蛋白异源复合物 异源复合物；蛋白 × 42 PDB 声明：42-meric（42） 与蛋白数一致 |
链 A
181–570（390 aa）
片段：E protein
链 B
181–570（390 aa）
片段：E protein
链 C
181–570（390 aa）
片段：E protein
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.6
cryo-EM玻璃化条件
冷冻剂 ETHANE；SAMPLES WERE PREPARED AS
THIN LAYERS OF VITREOUS ICE
AND MAINTAINED AT LIQUID
NITROGEN TEMPERATURE IN THE
ELECTRON MICROSCOPE
|
分辨率 24.00 Å |
| 2R6P Fit of E protein and Fab 1A1D-2 into 24 angstrom resolution cryoEM map of Fab complexed with dengue 2 virus. 提交 2007-09-06 | Assembly 5 蛋白异源复合物 异源复合物；蛋白 × 7 PDB 声明：heptameric（7） 与蛋白数一致 |
链 A
181–570（390 aa）
片段：E protein
链 B
181–570（390 aa）
片段：E protein
链 C
181–570（390 aa）
片段：E protein
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.6
cryo-EM玻璃化条件
冷冻剂 ETHANE；SAMPLES WERE PREPARED AS
THIN LAYERS OF VITREOUS ICE
AND MAINTAINED AT LIQUID
NITROGEN TEMPERATURE IN THE
ELECTRON MICROSCOPE
|
分辨率 24.00 Å |
| 3C6R Low pH Immature Dengue Virus 提交 2008-02-05 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 360 PDB 声明：360-meric（360） 与蛋白数一致 |
链 A
181–575（395 aa）
链 B
181–575（395 aa）
链 C
181–575（395 aa）
链 D
15–95（81 aa）
链 E
15–95（81 aa）
链 F
15–95（81 aa）
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 6；The virus was mixed in NTE buffer (10 mM Tris, 120 mM NaCl, and 1 mM EDTA at pH 8) with 50 mM MES, 120 mM NaCl at pH 5.6 to yield a final pH of 6
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 25.00 Å |
| 3C6R Low pH Immature Dengue Virus 提交 2008-02-05 | Assembly 2 蛋白同源多聚体 同源多聚体；蛋白 × 6 PDB 声明：hexameric（6） 与蛋白数一致 |
链 A
181–575（395 aa）
链 B
181–575（395 aa）
链 C
181–575（395 aa）
链 D
15–95（81 aa）
链 E
15–95（81 aa）
链 F
15–95（81 aa）
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 6；The virus was mixed in NTE buffer (10 mM Tris, 120 mM NaCl, and 1 mM EDTA at pH 8) with 50 mM MES, 120 mM NaCl at pH 5.6 to yield a final pH of 6
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 25.00 Å |
| 3C6R Low pH Immature Dengue Virus 提交 2008-02-05 | Assembly 3 蛋白同源多聚体 同源多聚体；蛋白 × 30 PDB 声明：30-meric（30） 与蛋白数一致 |
链 A
181–575（395 aa）
链 B
181–575（395 aa）
链 C
181–575（395 aa）
链 D
15–95（81 aa）
链 E
15–95（81 aa）
链 F
15–95（81 aa）
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 6；The virus was mixed in NTE buffer (10 mM Tris, 120 mM NaCl, and 1 mM EDTA at pH 8) with 50 mM MES, 120 mM NaCl at pH 5.6 to yield a final pH of 6
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 25.00 Å |
| 3C6R Low pH Immature Dengue Virus 提交 2008-02-05 | Assembly 4 蛋白同源多聚体 同源多聚体；蛋白 × 36 PDB 声明：36-meric（36） 与蛋白数一致 |
链 A
181–575（395 aa）
链 B
181–575（395 aa）
链 C
181–575（395 aa）
链 D
15–95（81 aa）
链 E
15–95（81 aa）
链 F
15–95（81 aa）
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 6；The virus was mixed in NTE buffer (10 mM Tris, 120 mM NaCl, and 1 mM EDTA at pH 8) with 50 mM MES, 120 mM NaCl at pH 5.6 to yield a final pH of 6
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 25.00 Å |
| 3C6R Low pH Immature Dengue Virus 提交 2008-02-05 | Assembly 5 蛋白同源多聚体 同源多聚体；蛋白 × 6 PDB 声明：hexameric（6） 与蛋白数一致 |
链 A
181–575（395 aa）
链 B
181–575（395 aa）
链 C
181–575（395 aa）
链 D
15–95（81 aa）
链 E
15–95（81 aa）
链 F
15–95（81 aa）
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 6；The virus was mixed in NTE buffer (10 mM Tris, 120 mM NaCl, and 1 mM EDTA at pH 8) with 50 mM MES, 120 mM NaCl at pH 5.6 to yield a final pH of 6
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 25.00 Å |
| 3IXY The pseudo-atomic structure of dengue immature virus in complex with Fab fragments of the anti-fusion loop antibody E53 提交 2009-02-26 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 600 PDB 声明：600-MERIC（600） 与蛋白数一致 |
链 D
15–95（81 aa）
链 E
15–95（81 aa）
链 F
15–95（81 aa）
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.6；12 mM Tris-HCl, 120 mM NaCl, 1 mM EDTA
cryo-EM玻璃化条件
A small vial of ethane is placed inside a larger liquid nitrogen reservoir. The grid holding a few microliters of the sample is held in place at the bottom of a plunger by the means of fine tweezers. Once the ethane in the vial is completely frozen, it needs to be slightly melted. When the liquid ethane is ready, a piece of filter paper is then pressed against the sample to blot of excess buffer, sufficient to leave a thin layer on the grid. After a predetermined time, the filter paper is removed, and the plunger is allowed to drop into the liquid ethane. Once the grid enters the liquid ethane, the sample is rapidly frozen, and the grid is transferred under liquid nitrogen to a storage box immersed liquid nitrogen for later use in the microscope.；冷冻剂 ETHANE
|
分辨率 23.00 Å |
| 3IXY The pseudo-atomic structure of dengue immature virus in complex with Fab fragments of the anti-fusion loop antibody E53 提交 2009-02-26 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 10 PDB 声明：decameric（10） 与蛋白数一致 |
链 D
15–95（81 aa）
链 E
15–95（81 aa）
链 F
15–95（81 aa）
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.6；12 mM Tris-HCl, 120 mM NaCl, 1 mM EDTA
cryo-EM玻璃化条件
A small vial of ethane is placed inside a larger liquid nitrogen reservoir. The grid holding a few microliters of the sample is held in place at the bottom of a plunger by the means of fine tweezers. Once the ethane in the vial is completely frozen, it needs to be slightly melted. When the liquid ethane is ready, a piece of filter paper is then pressed against the sample to blot of excess buffer, sufficient to leave a thin layer on the grid. After a predetermined time, the filter paper is removed, and the plunger is allowed to drop into the liquid ethane. Once the grid enters the liquid ethane, the sample is rapidly frozen, and the grid is transferred under liquid nitrogen to a storage box immersed liquid nitrogen for later use in the microscope.；冷冻剂 ETHANE
|
分辨率 23.00 Å |
| 3IXY The pseudo-atomic structure of dengue immature virus in complex with Fab fragments of the anti-fusion loop antibody E53 提交 2009-02-26 | Assembly 3 蛋白异源复合物 异源复合物；蛋白 × 50 PDB 声明：50-meric（50） 与蛋白数一致 |
链 D
15–95（81 aa）
链 E
15–95（81 aa）
链 F
15–95（81 aa）
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.6；12 mM Tris-HCl, 120 mM NaCl, 1 mM EDTA
cryo-EM玻璃化条件
A small vial of ethane is placed inside a larger liquid nitrogen reservoir. The grid holding a few microliters of the sample is held in place at the bottom of a plunger by the means of fine tweezers. Once the ethane in the vial is completely frozen, it needs to be slightly melted. When the liquid ethane is ready, a piece of filter paper is then pressed against the sample to blot of excess buffer, sufficient to leave a thin layer on the grid. After a predetermined time, the filter paper is removed, and the plunger is allowed to drop into the liquid ethane. Once the grid enters the liquid ethane, the sample is rapidly frozen, and the grid is transferred under liquid nitrogen to a storage box immersed liquid nitrogen for later use in the microscope.；冷冻剂 ETHANE
|
分辨率 23.00 Å |
| 3IXY The pseudo-atomic structure of dengue immature virus in complex with Fab fragments of the anti-fusion loop antibody E53 提交 2009-02-26 | Assembly 4 蛋白异源复合物 异源复合物；蛋白 × 60 PDB 声明：60-meric（60） 与蛋白数一致 |
链 D
15–95（81 aa）
链 E
15–95（81 aa）
链 F
15–95（81 aa）
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.6；12 mM Tris-HCl, 120 mM NaCl, 1 mM EDTA
cryo-EM玻璃化条件
A small vial of ethane is placed inside a larger liquid nitrogen reservoir. The grid holding a few microliters of the sample is held in place at the bottom of a plunger by the means of fine tweezers. Once the ethane in the vial is completely frozen, it needs to be slightly melted. When the liquid ethane is ready, a piece of filter paper is then pressed against the sample to blot of excess buffer, sufficient to leave a thin layer on the grid. After a predetermined time, the filter paper is removed, and the plunger is allowed to drop into the liquid ethane. Once the grid enters the liquid ethane, the sample is rapidly frozen, and the grid is transferred under liquid nitrogen to a storage box immersed liquid nitrogen for later use in the microscope.；冷冻剂 ETHANE
|
分辨率 23.00 Å |
| 3IXY The pseudo-atomic structure of dengue immature virus in complex with Fab fragments of the anti-fusion loop antibody E53 提交 2009-02-26 | Assembly 5 蛋白异源复合物 异源复合物；蛋白 × 10 PDB 声明：decameric（10） 与蛋白数一致 |
链 D
15–95（81 aa）
链 E
15–95（81 aa）
链 F
15–95（81 aa）
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.6；12 mM Tris-HCl, 120 mM NaCl, 1 mM EDTA
cryo-EM玻璃化条件
A small vial of ethane is placed inside a larger liquid nitrogen reservoir. The grid holding a few microliters of the sample is held in place at the bottom of a plunger by the means of fine tweezers. Once the ethane in the vial is completely frozen, it needs to be slightly melted. When the liquid ethane is ready, a piece of filter paper is then pressed against the sample to blot of excess buffer, sufficient to leave a thin layer on the grid. After a predetermined time, the filter paper is removed, and the plunger is allowed to drop into the liquid ethane. Once the grid enters the liquid ethane, the sample is rapidly frozen, and the grid is transferred under liquid nitrogen to a storage box immersed liquid nitrogen for later use in the microscope.；冷冻剂 ETHANE
|
分辨率 23.00 Å |
| 3IYA Association of the pr peptides with dengue virus blocks membrane fusion at acidic pH 提交 2009-06-01 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 360 PDB 声明：360-meric（360） 与蛋白数一致 |
链 A
181–575（395 aa）
链 B
181–575（395 aa）
链 C
181–575（395 aa）
链 D
15–95（81 aa）
链 E
15–95（81 aa）
链 F
15–95（81 aa）
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 6；6 mM Tris-HCL 25 mM MES 120 mM NaCl 1 mM EDTA
cryo-EM玻璃化条件
A small vial of ethane is placed inside a larger liquid nitrogen reservoir. The grid holding a few microliters of the sample is held in place at the bottom of a plunger by the means of fine tweezers. Once the ethane in the vial is completely frozen, it needs to be slightly melted. When the liquid ethane is ready, a piece of filter paper is then pressed against the sample to blot of excess buffer, sufficient to leave a thin layer on the grid. After a predetermined time, the filter paper is removed, and the plunger is allowed to drop into the liquid ethane. Once the grid enters the liquid ethane, the sample is rapidly frozen, and the grid is transferred under liquid nitrogen to a storage box immersed liquid nitrogen for later use in the microscope.；冷冻剂 ETHANE
|
分辨率 22.00 Å |
| 3IYA Association of the pr peptides with dengue virus blocks membrane fusion at acidic pH 提交 2009-06-01 | Assembly 2 蛋白同源多聚体 同源多聚体；蛋白 × 6 PDB 声明：hexameric（6） 与蛋白数一致 |
链 A
181–575（395 aa）
链 B
181–575（395 aa）
链 C
181–575（395 aa）
链 D
15–95（81 aa）
链 E
15–95（81 aa）
链 F
15–95（81 aa）
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 6；6 mM Tris-HCL 25 mM MES 120 mM NaCl 1 mM EDTA
cryo-EM玻璃化条件
A small vial of ethane is placed inside a larger liquid nitrogen reservoir. The grid holding a few microliters of the sample is held in place at the bottom of a plunger by the means of fine tweezers. Once the ethane in the vial is completely frozen, it needs to be slightly melted. When the liquid ethane is ready, a piece of filter paper is then pressed against the sample to blot of excess buffer, sufficient to leave a thin layer on the grid. After a predetermined time, the filter paper is removed, and the plunger is allowed to drop into the liquid ethane. Once the grid enters the liquid ethane, the sample is rapidly frozen, and the grid is transferred under liquid nitrogen to a storage box immersed liquid nitrogen for later use in the microscope.；冷冻剂 ETHANE
|
分辨率 22.00 Å |
| 3IYA Association of the pr peptides with dengue virus blocks membrane fusion at acidic pH 提交 2009-06-01 | Assembly 3 蛋白同源多聚体 同源多聚体；蛋白 × 30 PDB 声明：30-meric（30） 与蛋白数一致 |
链 A
181–575（395 aa）
链 B
181–575（395 aa）
链 C
181–575（395 aa）
链 D
15–95（81 aa）
链 E
15–95（81 aa）
链 F
15–95（81 aa）
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 6；6 mM Tris-HCL 25 mM MES 120 mM NaCl 1 mM EDTA
cryo-EM玻璃化条件
A small vial of ethane is placed inside a larger liquid nitrogen reservoir. The grid holding a few microliters of the sample is held in place at the bottom of a plunger by the means of fine tweezers. Once the ethane in the vial is completely frozen, it needs to be slightly melted. When the liquid ethane is ready, a piece of filter paper is then pressed against the sample to blot of excess buffer, sufficient to leave a thin layer on the grid. After a predetermined time, the filter paper is removed, and the plunger is allowed to drop into the liquid ethane. Once the grid enters the liquid ethane, the sample is rapidly frozen, and the grid is transferred under liquid nitrogen to a storage box immersed liquid nitrogen for later use in the microscope.；冷冻剂 ETHANE
|
分辨率 22.00 Å |
| 3IYA Association of the pr peptides with dengue virus blocks membrane fusion at acidic pH 提交 2009-06-01 | Assembly 4 蛋白同源多聚体 同源多聚体；蛋白 × 36 PDB 声明：36-meric（36） 与蛋白数一致 |
链 A
181–575（395 aa）
链 B
181–575（395 aa）
链 C
181–575（395 aa）
链 D
15–95（81 aa）
链 E
15–95（81 aa）
链 F
15–95（81 aa）
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 6；6 mM Tris-HCL 25 mM MES 120 mM NaCl 1 mM EDTA
cryo-EM玻璃化条件
A small vial of ethane is placed inside a larger liquid nitrogen reservoir. The grid holding a few microliters of the sample is held in place at the bottom of a plunger by the means of fine tweezers. Once the ethane in the vial is completely frozen, it needs to be slightly melted. When the liquid ethane is ready, a piece of filter paper is then pressed against the sample to blot of excess buffer, sufficient to leave a thin layer on the grid. After a predetermined time, the filter paper is removed, and the plunger is allowed to drop into the liquid ethane. Once the grid enters the liquid ethane, the sample is rapidly frozen, and the grid is transferred under liquid nitrogen to a storage box immersed liquid nitrogen for later use in the microscope.；冷冻剂 ETHANE
|
分辨率 22.00 Å |
| 3IYA Association of the pr peptides with dengue virus blocks membrane fusion at acidic pH 提交 2009-06-01 | Assembly 5 蛋白同源多聚体 同源多聚体；蛋白 × 6 PDB 声明：hexameric（6） 与蛋白数一致 |
链 A
181–575（395 aa）
链 B
181–575（395 aa）
链 C
181–575（395 aa）
链 D
15–95（81 aa）
链 E
15–95（81 aa）
链 F
15–95（81 aa）
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 6；6 mM Tris-HCL 25 mM MES 120 mM NaCl 1 mM EDTA
cryo-EM玻璃化条件
A small vial of ethane is placed inside a larger liquid nitrogen reservoir. The grid holding a few microliters of the sample is held in place at the bottom of a plunger by the means of fine tweezers. Once the ethane in the vial is completely frozen, it needs to be slightly melted. When the liquid ethane is ready, a piece of filter paper is then pressed against the sample to blot of excess buffer, sufficient to leave a thin layer on the grid. After a predetermined time, the filter paper is removed, and the plunger is allowed to drop into the liquid ethane. Once the grid enters the liquid ethane, the sample is rapidly frozen, and the grid is transferred under liquid nitrogen to a storage box immersed liquid nitrogen for later use in the microscope.；冷冻剂 ETHANE
|
分辨率 22.00 Å |