Current Protein Identity：P18356
New Search
Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 2JSF Solution structures of the envelope protein domain III from the dengue-2 virus Deposited 2007-07-03 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
469–577（109 aa）
Fragment：Residues 469-577
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7.4；298 K；Ionic strength (raw mmCIF value) 0.31；Pressure ambient
NMR sample composition
1-5 mM EDTA, 1-5 mM DTT, 1 mM sodium azide, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 2R69 Crystal structure of Fab 1A1D-2 complexed with E-DIII of Dengue virus at 3.8 angstrom resolution Deposited 2007-09-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
478–574（97 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.8；298 K；12% PEG 3350, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.80 Å R-free 0.363 |
| 2R6P Fit of E protein and Fab 1A1D-2 into 24 angstrom resolution cryoEM map of Fab complexed with dengue 2 virus. Deposited 2007-09-06 | Assembly 1 Protein heterocomplex Heteromer；Protein × 420 PDB declaration: 420-MERIC（420） Consistent with protein count |
Chain A
181–570（390 aa）
Fragment：E protein
Chain B
181–570（390 aa）
Fragment：E protein
Chain C
181–570（390 aa）
Fragment：E protein
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；SAMPLES WERE PREPARED AS
THIN LAYERS OF VITREOUS ICE
AND MAINTAINED AT LIQUID
NITROGEN TEMPERATURE IN THE
ELECTRON MICROSCOPE
|
Resolution 24.00 Å |
| 2R6P Fit of E protein and Fab 1A1D-2 into 24 angstrom resolution cryoEM map of Fab complexed with dengue 2 virus. Deposited 2007-09-06 | Assembly 2 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric（7） Consistent with protein count |
Chain A
181–570（390 aa）
Fragment：E protein
Chain B
181–570（390 aa）
Fragment：E protein
Chain C
181–570（390 aa）
Fragment：E protein
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；SAMPLES WERE PREPARED AS
THIN LAYERS OF VITREOUS ICE
AND MAINTAINED AT LIQUID
NITROGEN TEMPERATURE IN THE
ELECTRON MICROSCOPE
|
Resolution 24.00 Å |
| 2R6P Fit of E protein and Fab 1A1D-2 into 24 angstrom resolution cryoEM map of Fab complexed with dengue 2 virus. Deposited 2007-09-06 | Assembly 3 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric（35） Consistent with protein count |
Chain A
181–570（390 aa）
Fragment：E protein
Chain B
181–570（390 aa）
Fragment：E protein
Chain C
181–570（390 aa）
Fragment：E protein
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；SAMPLES WERE PREPARED AS
THIN LAYERS OF VITREOUS ICE
AND MAINTAINED AT LIQUID
NITROGEN TEMPERATURE IN THE
ELECTRON MICROSCOPE
|
Resolution 24.00 Å |
| 2R6P Fit of E protein and Fab 1A1D-2 into 24 angstrom resolution cryoEM map of Fab complexed with dengue 2 virus. Deposited 2007-09-06 | Assembly 4 Protein heterocomplex Heteromer；Protein × 42 PDB declaration: 42-meric（42） Consistent with protein count |
Chain A
181–570（390 aa）
Fragment：E protein
Chain B
181–570（390 aa）
Fragment：E protein
Chain C
181–570（390 aa）
Fragment：E protein
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；SAMPLES WERE PREPARED AS
THIN LAYERS OF VITREOUS ICE
AND MAINTAINED AT LIQUID
NITROGEN TEMPERATURE IN THE
ELECTRON MICROSCOPE
|
Resolution 24.00 Å |
| 2R6P Fit of E protein and Fab 1A1D-2 into 24 angstrom resolution cryoEM map of Fab complexed with dengue 2 virus. Deposited 2007-09-06 | Assembly 5 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric（7） Consistent with protein count |
Chain A
181–570（390 aa）
Fragment：E protein
Chain B
181–570（390 aa）
Fragment：E protein
Chain C
181–570（390 aa）
Fragment：E protein
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；SAMPLES WERE PREPARED AS
THIN LAYERS OF VITREOUS ICE
AND MAINTAINED AT LIQUID
NITROGEN TEMPERATURE IN THE
ELECTRON MICROSCOPE
|
Resolution 24.00 Å |
| 3C6R Low pH Immature Dengue Virus Deposited 2008-02-05 | Assembly 1 Protein homooligomer Homooligomer；Protein × 360 PDB declaration: 360-meric（360） Consistent with protein count |
Chain A
181–575（395 aa）
Chain B
181–575（395 aa）
Chain C
181–575（395 aa）
Chain D
15–95（81 aa）
Chain E
15–95（81 aa）
Chain F
15–95（81 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6；The virus was mixed in NTE buffer (10 mM Tris, 120 mM NaCl, and 1 mM EDTA at pH 8) with 50 mM MES, 120 mM NaCl at pH 5.6 to yield a final pH of 6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 25.00 Å |
| 3C6R Low pH Immature Dengue Virus Deposited 2008-02-05 | Assembly 2 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain A
181–575（395 aa）
Chain B
181–575（395 aa）
Chain C
181–575（395 aa）
Chain D
15–95（81 aa）
Chain E
15–95（81 aa）
Chain F
15–95（81 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6；The virus was mixed in NTE buffer (10 mM Tris, 120 mM NaCl, and 1 mM EDTA at pH 8) with 50 mM MES, 120 mM NaCl at pH 5.6 to yield a final pH of 6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 25.00 Å |
| 3C6R Low pH Immature Dengue Virus Deposited 2008-02-05 | Assembly 3 Protein homooligomer Homooligomer；Protein × 30 PDB declaration: 30-meric（30） Consistent with protein count |
Chain A
181–575（395 aa）
Chain B
181–575（395 aa）
Chain C
181–575（395 aa）
Chain D
15–95（81 aa）
Chain E
15–95（81 aa）
Chain F
15–95（81 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6；The virus was mixed in NTE buffer (10 mM Tris, 120 mM NaCl, and 1 mM EDTA at pH 8) with 50 mM MES, 120 mM NaCl at pH 5.6 to yield a final pH of 6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 25.00 Å |
| 3C6R Low pH Immature Dengue Virus Deposited 2008-02-05 | Assembly 4 Protein homooligomer Homooligomer；Protein × 36 PDB declaration: 36-meric（36） Consistent with protein count |
Chain A
181–575（395 aa）
Chain B
181–575（395 aa）
Chain C
181–575（395 aa）
Chain D
15–95（81 aa）
Chain E
15–95（81 aa）
Chain F
15–95（81 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6；The virus was mixed in NTE buffer (10 mM Tris, 120 mM NaCl, and 1 mM EDTA at pH 8) with 50 mM MES, 120 mM NaCl at pH 5.6 to yield a final pH of 6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 25.00 Å |
| 3C6R Low pH Immature Dengue Virus Deposited 2008-02-05 | Assembly 5 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain A
181–575（395 aa）
Chain B
181–575（395 aa）
Chain C
181–575（395 aa）
Chain D
15–95（81 aa）
Chain E
15–95（81 aa）
Chain F
15–95（81 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6；The virus was mixed in NTE buffer (10 mM Tris, 120 mM NaCl, and 1 mM EDTA at pH 8) with 50 mM MES, 120 mM NaCl at pH 5.6 to yield a final pH of 6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 25.00 Å |
| 3IXY The pseudo-atomic structure of dengue immature virus in complex with Fab fragments of the anti-fusion loop antibody E53 Deposited 2009-02-26 | Assembly 1 Protein heterocomplex Heteromer；Protein × 600 PDB declaration: 600-MERIC（600） Consistent with protein count |
Chain D
15–95（81 aa）
Chain E
15–95（81 aa）
Chain F
15–95（81 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6；12 mM Tris-HCl, 120 mM NaCl, 1 mM EDTA
cryo-EM vitrification conditions
A small vial of ethane is placed inside a larger liquid nitrogen reservoir. The grid holding a few microliters of the sample is held in place at the bottom of a plunger by the means of fine tweezers. Once the ethane in the vial is completely frozen, it needs to be slightly melted. When the liquid ethane is ready, a piece of filter paper is then pressed against the sample to blot of excess buffer, sufficient to leave a thin layer on the grid. After a predetermined time, the filter paper is removed, and the plunger is allowed to drop into the liquid ethane. Once the grid enters the liquid ethane, the sample is rapidly frozen, and the grid is transferred under liquid nitrogen to a storage box immersed liquid nitrogen for later use in the microscope.；Cryogen ETHANE
|
Resolution 23.00 Å |
| 3IXY The pseudo-atomic structure of dengue immature virus in complex with Fab fragments of the anti-fusion loop antibody E53 Deposited 2009-02-26 | Assembly 2 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric（10） Consistent with protein count |
Chain D
15–95（81 aa）
Chain E
15–95（81 aa）
Chain F
15–95（81 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6；12 mM Tris-HCl, 120 mM NaCl, 1 mM EDTA
cryo-EM vitrification conditions
A small vial of ethane is placed inside a larger liquid nitrogen reservoir. The grid holding a few microliters of the sample is held in place at the bottom of a plunger by the means of fine tweezers. Once the ethane in the vial is completely frozen, it needs to be slightly melted. When the liquid ethane is ready, a piece of filter paper is then pressed against the sample to blot of excess buffer, sufficient to leave a thin layer on the grid. After a predetermined time, the filter paper is removed, and the plunger is allowed to drop into the liquid ethane. Once the grid enters the liquid ethane, the sample is rapidly frozen, and the grid is transferred under liquid nitrogen to a storage box immersed liquid nitrogen for later use in the microscope.；Cryogen ETHANE
|
Resolution 23.00 Å |
| 3IXY The pseudo-atomic structure of dengue immature virus in complex with Fab fragments of the anti-fusion loop antibody E53 Deposited 2009-02-26 | Assembly 3 Protein heterocomplex Heteromer；Protein × 50 PDB declaration: 50-meric（50） Consistent with protein count |
Chain D
15–95（81 aa）
Chain E
15–95（81 aa）
Chain F
15–95（81 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6；12 mM Tris-HCl, 120 mM NaCl, 1 mM EDTA
cryo-EM vitrification conditions
A small vial of ethane is placed inside a larger liquid nitrogen reservoir. The grid holding a few microliters of the sample is held in place at the bottom of a plunger by the means of fine tweezers. Once the ethane in the vial is completely frozen, it needs to be slightly melted. When the liquid ethane is ready, a piece of filter paper is then pressed against the sample to blot of excess buffer, sufficient to leave a thin layer on the grid. After a predetermined time, the filter paper is removed, and the plunger is allowed to drop into the liquid ethane. Once the grid enters the liquid ethane, the sample is rapidly frozen, and the grid is transferred under liquid nitrogen to a storage box immersed liquid nitrogen for later use in the microscope.；Cryogen ETHANE
|
Resolution 23.00 Å |
| 3IXY The pseudo-atomic structure of dengue immature virus in complex with Fab fragments of the anti-fusion loop antibody E53 Deposited 2009-02-26 | Assembly 4 Protein heterocomplex Heteromer；Protein × 60 PDB declaration: 60-meric（60） Consistent with protein count |
Chain D
15–95（81 aa）
Chain E
15–95（81 aa）
Chain F
15–95（81 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6；12 mM Tris-HCl, 120 mM NaCl, 1 mM EDTA
cryo-EM vitrification conditions
A small vial of ethane is placed inside a larger liquid nitrogen reservoir. The grid holding a few microliters of the sample is held in place at the bottom of a plunger by the means of fine tweezers. Once the ethane in the vial is completely frozen, it needs to be slightly melted. When the liquid ethane is ready, a piece of filter paper is then pressed against the sample to blot of excess buffer, sufficient to leave a thin layer on the grid. After a predetermined time, the filter paper is removed, and the plunger is allowed to drop into the liquid ethane. Once the grid enters the liquid ethane, the sample is rapidly frozen, and the grid is transferred under liquid nitrogen to a storage box immersed liquid nitrogen for later use in the microscope.；Cryogen ETHANE
|
Resolution 23.00 Å |
| 3IXY The pseudo-atomic structure of dengue immature virus in complex with Fab fragments of the anti-fusion loop antibody E53 Deposited 2009-02-26 | Assembly 5 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric（10） Consistent with protein count |
Chain D
15–95（81 aa）
Chain E
15–95（81 aa）
Chain F
15–95（81 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6；12 mM Tris-HCl, 120 mM NaCl, 1 mM EDTA
cryo-EM vitrification conditions
A small vial of ethane is placed inside a larger liquid nitrogen reservoir. The grid holding a few microliters of the sample is held in place at the bottom of a plunger by the means of fine tweezers. Once the ethane in the vial is completely frozen, it needs to be slightly melted. When the liquid ethane is ready, a piece of filter paper is then pressed against the sample to blot of excess buffer, sufficient to leave a thin layer on the grid. After a predetermined time, the filter paper is removed, and the plunger is allowed to drop into the liquid ethane. Once the grid enters the liquid ethane, the sample is rapidly frozen, and the grid is transferred under liquid nitrogen to a storage box immersed liquid nitrogen for later use in the microscope.；Cryogen ETHANE
|
Resolution 23.00 Å |
| 3IYA Association of the pr peptides with dengue virus blocks membrane fusion at acidic pH Deposited 2009-06-01 | Assembly 1 Protein homooligomer Homooligomer；Protein × 360 PDB declaration: 360-meric（360） Consistent with protein count |
Chain A
181–575（395 aa）
Chain B
181–575（395 aa）
Chain C
181–575（395 aa）
Chain D
15–95（81 aa）
Chain E
15–95（81 aa）
Chain F
15–95（81 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6；6 mM Tris-HCL 25 mM MES 120 mM NaCl 1 mM EDTA
cryo-EM vitrification conditions
A small vial of ethane is placed inside a larger liquid nitrogen reservoir. The grid holding a few microliters of the sample is held in place at the bottom of a plunger by the means of fine tweezers. Once the ethane in the vial is completely frozen, it needs to be slightly melted. When the liquid ethane is ready, a piece of filter paper is then pressed against the sample to blot of excess buffer, sufficient to leave a thin layer on the grid. After a predetermined time, the filter paper is removed, and the plunger is allowed to drop into the liquid ethane. Once the grid enters the liquid ethane, the sample is rapidly frozen, and the grid is transferred under liquid nitrogen to a storage box immersed liquid nitrogen for later use in the microscope.；Cryogen ETHANE
|
Resolution 22.00 Å |
| 3IYA Association of the pr peptides with dengue virus blocks membrane fusion at acidic pH Deposited 2009-06-01 | Assembly 2 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain A
181–575（395 aa）
Chain B
181–575（395 aa）
Chain C
181–575（395 aa）
Chain D
15–95（81 aa）
Chain E
15–95（81 aa）
Chain F
15–95（81 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6；6 mM Tris-HCL 25 mM MES 120 mM NaCl 1 mM EDTA
cryo-EM vitrification conditions
A small vial of ethane is placed inside a larger liquid nitrogen reservoir. The grid holding a few microliters of the sample is held in place at the bottom of a plunger by the means of fine tweezers. Once the ethane in the vial is completely frozen, it needs to be slightly melted. When the liquid ethane is ready, a piece of filter paper is then pressed against the sample to blot of excess buffer, sufficient to leave a thin layer on the grid. After a predetermined time, the filter paper is removed, and the plunger is allowed to drop into the liquid ethane. Once the grid enters the liquid ethane, the sample is rapidly frozen, and the grid is transferred under liquid nitrogen to a storage box immersed liquid nitrogen for later use in the microscope.；Cryogen ETHANE
|
Resolution 22.00 Å |
| 3IYA Association of the pr peptides with dengue virus blocks membrane fusion at acidic pH Deposited 2009-06-01 | Assembly 3 Protein homooligomer Homooligomer；Protein × 30 PDB declaration: 30-meric（30） Consistent with protein count |
Chain A
181–575（395 aa）
Chain B
181–575（395 aa）
Chain C
181–575（395 aa）
Chain D
15–95（81 aa）
Chain E
15–95（81 aa）
Chain F
15–95（81 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6；6 mM Tris-HCL 25 mM MES 120 mM NaCl 1 mM EDTA
cryo-EM vitrification conditions
A small vial of ethane is placed inside a larger liquid nitrogen reservoir. The grid holding a few microliters of the sample is held in place at the bottom of a plunger by the means of fine tweezers. Once the ethane in the vial is completely frozen, it needs to be slightly melted. When the liquid ethane is ready, a piece of filter paper is then pressed against the sample to blot of excess buffer, sufficient to leave a thin layer on the grid. After a predetermined time, the filter paper is removed, and the plunger is allowed to drop into the liquid ethane. Once the grid enters the liquid ethane, the sample is rapidly frozen, and the grid is transferred under liquid nitrogen to a storage box immersed liquid nitrogen for later use in the microscope.；Cryogen ETHANE
|
Resolution 22.00 Å |
| 3IYA Association of the pr peptides with dengue virus blocks membrane fusion at acidic pH Deposited 2009-06-01 | Assembly 4 Protein homooligomer Homooligomer；Protein × 36 PDB declaration: 36-meric（36） Consistent with protein count |
Chain A
181–575（395 aa）
Chain B
181–575（395 aa）
Chain C
181–575（395 aa）
Chain D
15–95（81 aa）
Chain E
15–95（81 aa）
Chain F
15–95（81 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6；6 mM Tris-HCL 25 mM MES 120 mM NaCl 1 mM EDTA
cryo-EM vitrification conditions
A small vial of ethane is placed inside a larger liquid nitrogen reservoir. The grid holding a few microliters of the sample is held in place at the bottom of a plunger by the means of fine tweezers. Once the ethane in the vial is completely frozen, it needs to be slightly melted. When the liquid ethane is ready, a piece of filter paper is then pressed against the sample to blot of excess buffer, sufficient to leave a thin layer on the grid. After a predetermined time, the filter paper is removed, and the plunger is allowed to drop into the liquid ethane. Once the grid enters the liquid ethane, the sample is rapidly frozen, and the grid is transferred under liquid nitrogen to a storage box immersed liquid nitrogen for later use in the microscope.；Cryogen ETHANE
|
Resolution 22.00 Å |
| 3IYA Association of the pr peptides with dengue virus blocks membrane fusion at acidic pH Deposited 2009-06-01 | Assembly 5 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain A
181–575（395 aa）
Chain B
181–575（395 aa）
Chain C
181–575（395 aa）
Chain D
15–95（81 aa）
Chain E
15–95（81 aa）
Chain F
15–95（81 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6；6 mM Tris-HCL 25 mM MES 120 mM NaCl 1 mM EDTA
cryo-EM vitrification conditions
A small vial of ethane is placed inside a larger liquid nitrogen reservoir. The grid holding a few microliters of the sample is held in place at the bottom of a plunger by the means of fine tweezers. Once the ethane in the vial is completely frozen, it needs to be slightly melted. When the liquid ethane is ready, a piece of filter paper is then pressed against the sample to blot of excess buffer, sufficient to leave a thin layer on the grid. After a predetermined time, the filter paper is removed, and the plunger is allowed to drop into the liquid ethane. Once the grid enters the liquid ethane, the sample is rapidly frozen, and the grid is transferred under liquid nitrogen to a storage box immersed liquid nitrogen for later use in the microscope.；Cryogen ETHANE
|
Resolution 22.00 Å |