Current Protein Identity：P26368
New Search
Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1JMT X-ray Structure of a Core U2AF65/U2AF35 Heterodimer Deposited 2001-07-19 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
85–112（28 aa）
|
Not recorded | HEZ HEXANE-1,6-DIOL × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.3；277 K；PEG mme5000, sodium acetate, 1,6-hexanediol, MES, pH 5.3, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 2.20 Å R-free 0.238 |
| 1O0P Solution Structure of the third RNA Recognition Motif (RRM) of U2AF65 in complex with an N-terminal SF1 peptide Deposited 2003-02-24 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
372–475（104 aa）
Fragment：C-terminal RRM domain
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 6.4；295 K；Ionic strength (raw mmCIF value) 50mM salt；Pressure ambient
NMR sample composition
1mM 15N,13C U2AF65-RRM3 + 1mM unlabeled SF1_10-25, 30mM phosphate buffer, 20mM NaCL, 3mM DTT | 90% H2O/10% D2O
NMR sample composition
1mM 15N,13C U2AF65-RRM3 + 1mM unlabeled SF1_10-25, 30mM phosphate buffer, 20mM NaCL, 3mM DTT | 100% D2O
|
Resolution not provided |
| 1OPI SOLUTION STRUCTURE OF THE THIRD RNA RECOGNITION MOTIF (RRM) OF U2AF65 IN COMPLEX WITH AN N-TERMINAL SF1 PEPTIDE Deposited 2003-03-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
372–475（104 aa）
Fragment：C-TERMINAL RRM DOMAIN
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 6.4；295 K；Ionic strength (raw mmCIF value) 50mM SALT；Pressure AMBIENT
NMR sample composition
1MM 15N,13C U2AF65-RRM3 + 1MM UNLABELED SF1_10-25, 30MM PHOSPHATE BUFFER, 20MM NACL, 3MM DTT | D2O
|
Resolution not provided |
| 1U2F SOLUTION STRUCTURE OF THE FIRST RNA-BINDING DOMAIN OF HU2AF65 Deposited 1999-05-26 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
148–237（90 aa）
Fragment：FIRST RNA-BINDING DOMAIN
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7.5；298 K
|
Resolution not provided |
| 2G4B Structure of U2AF65 variant with polyuridine tract Deposited 2006-02-21 | Assembly 1 Protein–RNA Homooligomer；Protein × 2 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain A
148–237（90 aa）
Fragment：RNA binding domain
Chain A
258–336（79 aa）
Fragment：RNA binding domain
|
Mutation：deletion of residues 238-258 Mutation：deletion of residues 238-258 | DIO 1,4-DIETHYLENE DIOXIDE × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；.6 M ammonium sulfate, 100 mM 2-(N-morpholino) ethanesulfonic acid, sodium salt (MES) pH 6.5, 10% dioxane, 200 mM non-detergent sulfobetaine (NDSB) 195 , VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.50 Å R-free 0.276 |
| 2HZC Crystal structure of the N-terminal RRM of the U2AF large subunit Deposited 2006-08-08 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
148–229（82 aa）
Fragment：RRM 1
|
Not recorded | ZN ZINC ION × 2 P6G HEXAETHYLENE GLYCOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；21% PEGmme550, 200mM Zn acetate, 0.1M MES pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.47 Å R-free 0.226 |
| 2HZC Crystal structure of the N-terminal RRM of the U2AF large subunit Deposited 2006-08-08 | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
148–229（82 aa）
Fragment：RRM 1
|
Not recorded | ZN ZINC ION × 4 P6G HEXAETHYLENE GLYCOL × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；21% PEGmme550, 200mM Zn acetate, 0.1M MES pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.47 Å R-free 0.226 |
| 2M0G Structure, phosphorylation and U2AF65 binding of the Nterminal Domain of splicing factor 1 during 3 splice site Recognition Deposited 2012-10-25 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
372–475（104 aa）
Fragment：RRM 3 domain residues 372-475
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 6.5；298 K；Ionic strength (raw mmCIF value) 70；Pressure ambient
NMR sample composition
100-600 uM [U-15N] protein, 100-600 uM [U-13C; U-15N] entity, 100-600 uM [U-13C; U-15N; U-2H] entity, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 2U2F SOLUTION STRUCTURE OF THE SECOND RNA-BINDING DOMAIN OF HU2AF65 Deposited 1999-05-26 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
258–342（85 aa）
Fragment：SECOND RNA-BINDING DOMAIN
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 6.5；298 K
|
Resolution not provided |
| 2YH0 Solution structure of the closed conformation of human U2AF65 tandem RRM1 and RRM2 domains Deposited 2011-04-26 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
148–342（195 aa）
Fragment：TANDEM RRM1 AND RRM2 DOMAINS JOINED BY NATIVE LINKER, RESIDUES 148-342
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 6.5；295 K；Ionic strength (raw mmCIF value) 70；Pressure 1.0
NMR sample composition
90% WATER/10% D2O
|
Resolution not provided |
| 2YH1 Model of human U2AF65 tandem RRM1 and RRM2 domains with eight-site uridine binding Deposited 2011-04-26 | Assembly 1 Protein–RNA Monomer；Protein × 1 PDB declaration: dimeric（2） Consistent with all polymers |
Chain A
148–342（195 aa）
Fragment：TANDEM RRM1 AND RRM2 DOMAINS JOINED BY NATIVE LINKER, RESIDUES 148-342
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 6.5；295 K；Ionic strength (raw mmCIF value) 70；Pressure 1.0
NMR measurement conditions
pH 6.5；295 K；Ionic strength (raw mmCIF value) 70；Pressure 1.0
NMR measurement conditions
pH 6.5；295 K；Ionic strength (raw mmCIF value) 70；Pressure 1.0
NMR sample composition
90% WATER/10% D2O
|
Resolution not provided |
| 3VAF Structure of U2AF65 variant with BrU3 DNA Deposited 2011-12-29 | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain A
148–237（90 aa）
Fragment：RNA Binding Domains 1 and 2
Chain A
258–336（79 aa）
Fragment：RNA Binding Domains 1 and 2
Chain B
148–237（90 aa）
Fragment：RNA Binding Domains 1 and 2
Chain B
258–336（79 aa）
Fragment：RNA Binding Domains 1 and 2
|
Not recorded | DIO 1,4-DIETHYLENE DIOXIDE × 4 SO4 SULFATE ION × 6 GOL GLYCEROL × 1 CPQ N,N-BIS(3-D-GLUCONAMIDOPROPYL)DEOXYCHOLAMIDE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；1.6M Ammonium sulfate, 10% Dioxane, 0.1M MES pH 6.5, Deoxy-Big Chap, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.49 Å R-free 0.289 |
| 3VAG Structure of U2AF65 variant with BrU3C2 DNA Deposited 2011-12-29 | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain A
148–237（90 aa）
Fragment：RNA Binding Domains 1 and 2
Chain A
258–336（79 aa）
Fragment：RNA Binding Domains 1 and 2
Chain B
148–237（90 aa）
Fragment：RNA Binding Domains 1 and 2
Chain B
258–336（79 aa）
Fragment：RNA Binding Domains 1 and 2
|
Not recorded | DIO 1,4-DIETHYLENE DIOXIDE × 6 SO4 SULFATE ION × 7 GOL GLYCEROL × 2 CPQ N,N-BIS(3-D-GLUCONAMIDOPROPYL)DEOXYCHOLAMIDE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；1.6M Ammonium sulfate, 10% Dioxane, 0.1M MES pH 6.5, Deoxy-Big Chap, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.19 Å R-free 0.232 |
| 3VAH Structure of U2AF65 variant with BrU3C4 DNA Deposited 2011-12-29 | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain A
148–237（90 aa）
Fragment：RNA Binding Domains 1 and 2
Chain A
258–336（79 aa）
Fragment：RNA Binding Domains 1 and 2
Chain B
148–237（90 aa）
Fragment：RNA Binding Domains 1 and 2
Chain B
258–336（79 aa）
Fragment：RNA Binding Domains 1 and 2
|
Not recorded | DIO 1,4-DIETHYLENE DIOXIDE × 4 SO4 SULFATE ION × 5 CPQ N,N-BIS(3-D-GLUCONAMIDOPROPYL)DEOXYCHOLAMIDE × 1 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；1.6M Ammonium sulfate, 10% Dioxane, 0.1M MES pH 6.5, Deoxy-Big Chap, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.50 Å R-free 0.259 |
| 3VAI Structure of U2AF65 variant with BrU3C5 DNA Deposited 2011-12-29 | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain A
148–237（90 aa）
Fragment：RNA Binding Domains 1 and 2
Chain A
258–336（79 aa）
Fragment：RNA Binding Domains 1 and 2
Chain B
148–237（90 aa）
Fragment：RNA Binding Domains 1 and 2
Chain B
258–336（79 aa）
Fragment：RNA Binding Domains 1 and 2
|
Not recorded | DIO 1,4-DIETHYLENE DIOXIDE × 5 SO4 SULFATE ION × 7 CPQ N,N-BIS(3-D-GLUCONAMIDOPROPYL)DEOXYCHOLAMIDE × 1 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；1.6M M Ammonium sulfate, 10% Dioxane, 0.1M MES pH 6.5, Deoxy-Big Chap, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.20 Å R-free 0.250 |
| 3VAJ Structure of U2AF65 variant with BrU5C6 DNA Deposited 2011-12-29 | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain A
148–237（90 aa）
Fragment：RNA Binding Domains 1 and 2
Chain A
258–336（79 aa）
Fragment：RNA Binding Domains 1 and 2
Chain B
148–237（90 aa）
Fragment：RNA Binding Domains 1 and 2
Chain B
258–336（79 aa）
Fragment：RNA Binding Domains 1 and 2
|
Not recorded | DIO 1,4-DIETHYLENE DIOXIDE × 5 SO4 SULFATE ION × 6 GOL GLYCEROL × 2 CPQ N,N-BIS(3-D-GLUCONAMIDOPROPYL)DEOXYCHOLAMIDE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；1.6M Ammonium sulfate, 10% Dioxane, 0.1M MES pH 6.5, Deoxy-Big Chap, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.90 Å R-free 0.236 |
| 3VAK Structure of U2AF65 variant with BrU5 DNA Deposited 2011-12-29 | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain A
148–237（90 aa）
Fragment：RNA Binding Domains 1 and 2
Chain A
258–336（79 aa）
Fragment：RNA Binding Domains 1 and 2
Chain B
148–237（90 aa）
Fragment：RNA Binding Domains 1 and 2
Chain B
258–336（79 aa）
Fragment：RNA Binding Domains 1 and 2
|
Not recorded | DIO 1,4-DIETHYLENE DIOXIDE × 5 SO4 SULFATE ION × 7 GOL GLYCEROL × 4 CPQ N,N-BIS(3-D-GLUCONAMIDOPROPYL)DEOXYCHOLAMIDE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；1.6M Ammonium sulfate, 10% Dioxane, 0.1M MES pH 6.5, Deoxy-Big Chap, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.17 Å R-free 0.229 |
| 3VAL Structure of U2AF65 variant with BrU5C1 DNA Deposited 2011-12-29 | Assembly 1 Protein–DNA Homooligomer；Protein × 4 PDB declaration: octameric（8） Consistent with all polymers |
Chain A
148–237（90 aa）
Fragment：RNA Binding Domains 1 and 2
Chain A
258–336（79 aa）
Fragment：RNA Binding Domains 1 and 2
Chain B
148–237（90 aa）
Fragment：RNA Binding Domains 1 and 2
Chain B
258–336（79 aa）
Fragment：RNA Binding Domains 1 and 2
Chain D
148–237（90 aa）
Fragment：RNA Binding Domains 1 and 2
Chain D
258–336（79 aa）
Fragment：RNA Binding Domains 1 and 2
Chain I
148–237（90 aa）
Fragment：RNA Binding Domains 1 and 2
Chain I
258–336（79 aa）
Fragment：RNA Binding Domains 1 and 2
|
Not recorded | DIO 1,4-DIETHYLENE DIOXIDE × 4 SO4 SULFATE ION × 11 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；1.6M Ammonium sulfate, 10% Dioxane, 0.1M MES pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.50 Å R-free 0.271 |
| 3VAM Structure of U2AF65 variant with BrU5C2 DNA Deposited 2011-12-29 | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain A
148–237（90 aa）
Fragment：RNA Binding Domains 1 and 2
Chain A
258–336（79 aa）
Fragment：RNA Binding Domains 1 and 2
Chain B
148–237（90 aa）
Fragment：RNA Binding Domains 1 and 2
Chain B
258–336（79 aa）
Fragment：RNA Binding Domains 1 and 2
|
Not recorded | DIO 1,4-DIETHYLENE DIOXIDE × 5 SO4 SULFATE ION × 5 GOL GLYCEROL × 2 CPQ N,N-BIS(3-D-GLUCONAMIDOPROPYL)DEOXYCHOLAMIDE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；1.6M Ammonium sulfate, 10% Dioxane, 0.1M MES pH 6.5, Deoxy-Big Chap, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.40 Å R-free 0.237 |
| 4FXW Structure of phosphorylated SF1 complex with U2AF65-UHM domain Deposited 2012-07-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
375–475（101 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；0.2 M Lithium sulfate, 20% PEG 3350 and 0.1 M Tris pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.29 Å R-free 0.281 |
| 4FXW Structure of phosphorylated SF1 complex with U2AF65-UHM domain Deposited 2012-07-03 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
375–475（101 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；0.2 M Lithium sulfate, 20% PEG 3350 and 0.1 M Tris pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.29 Å R-free 0.281 |
| 4TU7 Structure of U2AF65 D231V variant with BrU5 DNA Deposited 2014-06-24 | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain A
148–336（189 aa）
Chain B
148–336（189 aa）
|
Mutation：D231V Mutation：D231V | DIO 1,4-DIETHYLENE DIOXIDE × 4 SO4 SULFATE ION × 6 GOL GLYCEROL × 4 CPQ N,N-BIS(3-D-GLUCONAMIDOPROPYL)DEOXYCHOLAMIDE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；1.6M AMMONIUM SULFATE, 10% DIOXANE, 0.1M MES PH 6.0, DEOXY-BIG CHAP
|
Resolution 2.09 Å R-free 0.223 |
| 4TU8 STRUCTURE OF U2AF65 VARIANT WITH BRU5A6 DNA Deposited 2014-06-24 | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain A
148–336（189 aa）
Fragment：UNP residues 148-336
Chain B
148–336（189 aa）
Fragment：UNP residues 148-336
|
Not recorded | DIO 1,4-DIETHYLENE DIOXIDE × 7 GOL GLYCEROL × 1 EDO 1,2-ETHANEDIOL × 9 SO4 SULFATE ION × 3 CPQ N,N-BIS(3-D-GLUCONAMIDOPROPYL)DEOXYCHOLAMIDE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；1.6M AMMONIUM SULFATE, 10% DIOXANE, 0.1M MES PH 6.5, ETHYLENE GLYCOL, DEOXY-BIG CHAP
|
Resolution 1.92 Å R-free 0.218 |
| 4TU9 STRUCTURE OF U2AF65 VARIANT WITH BRU5G6 DNA Deposited 2014-06-24 | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain A
148–336（189 aa）
Fragment：UNP residues 148-336
Chain B
148–336（189 aa）
Fragment：UNP residues 148-336
|
Not recorded | DIO 1,4-DIETHYLENE DIOXIDE × 5 SO4 SULFATE ION × 6 GOL GLYCEROL × 2 CPQ N,N-BIS(3-D-GLUCONAMIDOPROPYL)DEOXYCHOLAMIDE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；1.6M AMMONIUM SULFATE, 10% DIOXANE, 0.1M MES PH 6.5, DEOXY-BIG CHAP
|
Resolution 1.99 Å R-free 0.234 |
| 5EV1 Structure I of Intact U2AF65 Recognizing a 3' Splice Site Signal Deposited 2015-11-19 | Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: dimeric（2） Consistent with all polymers |
Chain A
141–341（201 aa）
Fragment：UNP residues 141-341
|
Not recorded | NA SODIUM ION × 2 PGE TRIETHYLENE GLYCOL × 1 PEG DI(HYDROXYETHYL)ETHER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；100 mM phosphate-citrate buffer, 40% Peg400, 10% dioxane
|
Resolution 2.04 Å R-free 0.228 |
| 5EV2 Structure II of Intact U2AF65 Recognizing the 3' Splice Site Signal Deposited 2015-11-19 | Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: dimeric（2） Consistent with all polymers |
Chain A
141–341（201 aa）
Fragment：UNP residues 141-341
|
Not recorded | DIO 1,4-DIETHYLENE DIOXIDE × 1 PGE TRIETHYLENE GLYCOL × 1 PEG DI(HYDROXYETHYL)ETHER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；200 mM LiCl, 100 mM sodium citrate, 8% PEG 6000, 10% PEG 300, 10% Dioxane, Deoxy-BigCHAP
|
Resolution 1.86 Å R-free 0.188 |
| 5EV3 Structure III of Intact U2AF65 Recognizing the 3' Splice Site Signal Deposited 2015-11-19 | Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: dimeric（2） Consistent with all polymers |
Chain A
141–341（201 aa）
Fragment：UNP residues 141-341
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；1M Succinic Acid, 0.1M, 1% PEG MME 2000
|
Resolution 1.50 Å R-free 0.186 |
| 5EV4 Structure IV of Intact U2AF65 Recognizing the 3' Splice Site Signal Deposited 2015-11-19 | Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: dimeric（2） Consistent with all polymers |
Chain A
141–341（201 aa）
Fragment：UNP RESIDUES 141-341
|
Not recorded | GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；1M Succinic Acid, 0.1M HEPES, 3% PEG MME 2000
|
Resolution 1.57 Å R-free 0.176 |
| 5W0G Structure of U2AF65 (U2AF2) RRM1 at 1.07 resolution Deposited 2017-05-30 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
148–229（82 aa）
Fragment：RNA RECOGNITION motif 1 (RRM1), residues 148-229
|
Not recorded | ZN ZINC ION × 2 ACT ACETATE ION × 1 1PG 2-(2-{2-[2-(2-METHOXY-ETHOXY)-ETHOXY]-ETHOXY}-ETHOXY)-ETHANOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；21% (w/v) PEG MME 550, 210 mM zinc acetate, 100 mM MES
|
Resolution 1.07 Å R-free 0.153 |
| 5W0H Structure of U2AF65 (U2AF2) RRM2 at 1.11 Angstrom Resolution Deposited 2017-05-30 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
258–336（79 aa）
Fragment：RNA Recognition Motif 2 (RRM2), residues 258-336
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；277 K；26% (w/v) PEG 8000, 0.1 M NaOAc, 0.2 mM TCEP, 10% (v/v) dioxane, 0.2 ul BigCHAP, 0.05 M potassium phosphate monobasic
|
Resolution 1.11 Å R-free 0.168 |
| 6TR0 Solution structure of U2AF2 RRM1,2 Deposited 2019-12-17 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
140–342（203 aa）
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 6.5；298 K；Ionic strength (raw mmCIF value) 50；Pressure 1
NMR sample composition
0.5 mM [U-13C; U-15N] U2AF2, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 6XLV Crystal structure of leukemia-associated N196K mutant of U2AF65 bound to AdML splice site Deposited 2020-06-29 | Assembly 1 Protein–RNA Monomer；Protein × 1 PDB declaration: dimeric（2） Consistent with all polymers |
Chain A
141–341（201 aa）
|
Mutation：N196K | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；PEG 3350, 3.4 M sodium malonate pH 7, sucrose, LDAO
|
Resolution 1.40 Å R-free 0.169 |
| 6XLW Crystal structure of U2AF65 bound to AdML splice site sequence Deposited 2020-06-29 | Assembly 1 Protein–RNA Monomer；Protein × 1 PDB declaration: dimeric（2） Consistent with all polymers |
Chain A
141–341（201 aa）
|
Not recorded | GOL GLYCEROL × 1 PEG DI(HYDROXYETHYL)ETHER × 1 NA SODIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277.15 K；1M Succinic Acid, 0.1M HEPES pH 7, 3% PEG MME 2000
|
Resolution 1.50 Å R-free 0.153 |
| 6XLX Crystal structure of cancer-associated G301D mutant of U2AF65 bound to AdML splice site Deposited 2020-06-29 | Assembly 1 Protein–RNA Monomer；Protein × 1 PDB declaration: dimeric（2） Consistent with all polymers |
Chain A
141–341（201 aa）
|
Mutation：G301D | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；25% PEG 3350, 0.24 M Na malonate pH 7, 5% sucrose, Lauryldimethylamine oxide
|
Resolution 1.70 Å R-free 0.194 |
| 7S3A Crystal structure of intact U2AF65 RRM-region bound to AdML-C5 oligonucleotide Deposited 2021-09-05 | Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: dimeric（2） Consistent with all polymers |
Chain A
141–341（201 aa）
|
Not recorded | NA SODIUM ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277.15 K；0.60 M succinic acid, 0.10 M Hepes pH 7.0, 2% Peg MME 2000
|
Resolution 1.48 Å R-free 0.157 |
| 7S3B Crystal structure of intact U2AF65 RRM-region bound to AdML-G5 oligonucleotide Deposited 2021-09-05 | Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: dimeric（2） Consistent with all polymers |
Chain A
141–341（201 aa）
|
Not recorded | PEG DI(HYDROXYETHYL)ETHER × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277.15 K；0.24 M Na Malonate, 26% Peg 3350, n-dodecyl-N,N-dimethylamine-N-oxide (=LDAO)
|
Resolution 1.89 Å R-free 0.200 |
| 7S3C Crystal structure of intact U2AF65 RRM-region bound to AdML-A5 oligonucleotide Deposited 2021-09-05 | Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: dimeric（2） Consistent with all polymers |
Chain A
141–341（201 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277.15 K；0.24 M malonate, 26% Peg 3350, 10% sucrose, LDAO
|
Resolution 1.51 Å R-free 0.190 |
| 7SN6 U2AF65 UHM BOUND TO SF3B155 ULM5 Deposited 2021-10-27 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
375–475（101 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；0.1 M SODIUM CITRATE TRIBASIC DIHYDRATE PH 5.6, 15% V/V 2-PROPANOL, 20% PEG 4000
|
Resolution 1.80 Å R-free 0.217 |
| 7SN6 U2AF65 UHM BOUND TO SF3B155 ULM5 Deposited 2021-10-27 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
375–475（101 aa）
|
Not recorded | IPA ISOPROPYL ALCOHOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；0.1 M SODIUM CITRATE TRIBASIC DIHYDRATE PH 5.6, 15% V/V 2-PROPANOL, 20% PEG 4000
|
Resolution 1.80 Å R-free 0.217 |
| 8P25 Solution structure of a chimeric U2AF2 RRM2 / FUBP1 N-Box Deposited 2023-05-14 | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
231–342（112 aa）
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 6.5；298 K；Ionic strength (raw mmCIF value) 50mM NaCl；Pressure 1
NMR sample composition
0.6 mM [U-100% 13C; U-100% 15N] Chimeric construct of U2AF2 linker-RRM2 and FUBP1 N-box, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
0.6 mM [U-100% 13C; U-100% 15N] Chimeric construct of U2AF2 linker-RRM2 and FUBP1 N-box, 100% D2O | 100% D2O
|
Resolution not provided |
| 9C7A Crystal structure of R149W neurodevelopmental disease-associated U2AF2 variant Deposited 2024-06-10 | Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: dimeric（2） Consistent with all polymers |
Chain A
141–341（201 aa）
|
Mutation：R149W | GOL GLYCEROL × 1 NA SODIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277.15 K；24 M sodium malonate pH 7.0, 5% w/v sucrose, 25% w/v PEG 3350, with addition of 0.1 uL of 5% w/v LDAO detergent
|
Resolution 1.40 Å R-free 0.159 |
| 9C7B Crystal structure of R150H neurodevelopmental disease-associated U2AF2 variant Deposited 2024-06-10 | Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: dimeric（2） Consistent with all polymers |
Chain A
141–341（201 aa）
|
Mutation：R150H | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；277.15 K；24 M sodium malonate pH 7.0, 5% w/v sucrose, 25% w/v PEG 3350, with addition of 0.1 uL of 5% w/v LDAO detergent
|
Resolution 1.40 Å R-free 0.167 |
| 9DWU CoREST complex bound to U2AF2 Deposited 2024-10-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain C
257–336（80 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.14 Å |