Current Protein Identity：P28320
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 5GMK Cryo-EM structure of the Catalytic Step I spliceosome (C complex) at 3.4 angstrom resolution Deposited 2016-07-14 | Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 45-meric（45） Consistent with all polymers |
Chain F
1–278（278 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8；The CEB buffer (10 mM Tris-HCl, pH 8.0, 75 mM NaCl, 1 mM Mg(OAc)2, 1 mM imidazole, 0.01% NP40, 1 mM TCEP, 0.5 mM EGTA)
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 5LJ3 Structure of the core of the yeast spliceosome immediately after branching Deposited 2016-07-17 | Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 38-meric（38） Consistent with all polymers |
Chain D
1–278（278 aa）
|
Not recorded | MG MAGNESIUM ION × 2 ZN ZINC ION × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.8
cryo-EM vitrification conditions
Cryogen ETHANE；3 microlitres sample were applied to the grid, left for 30 seconds and then blotted for 2.5-3.0 seconds before plunging.
|
Resolution 3.80 Å |
| 5LJ5 Overall structure of the yeast spliceosome immediately after branching. Deposited 2016-07-17 | Assembly 1 Protein–RNA Heteromer；Protein × 40 PDB declaration: 45-meric（45） Consistent with all polymers |
Chain D
1–278（278 aa）
|
Not recorded | MG MAGNESIUM ION × 2 ZN ZINC ION × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.8
cryo-EM vitrification conditions
Cryogen ETHANE；3 microlitres sample were applied to the grid, left for 30 seconds and then blotted for 2.5-3.0 seconds before plunging.
|
Resolution 10.00 Å |
| 5Y88 Cryo-EM structure of the intron-lariat spliceosome ready for disassembly from S.cerevisiae at 3.5 angstrom Deposited 2017-08-20 | Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 44-meric（44） Consistent with all polymers |
Chain R
1–278（278 aa）
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.46 Å |
| 6EXN Post-catalytic P complex spliceosome with 3' splice site docked Deposited 2017-11-08 | Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 40-meric（40） Review required |
Chain D
1–278（278 aa）
|
Not recorded | MG MAGNESIUM ION × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE；3 uL sample was applied to the grid, left for 30s, then blotted for 3s and immediately plunged into liquid ethane.
|
Resolution 3.70 Å |
| 6J6Q Cryo-EM structure of the yeast B*-b2 complex at an average resolution of 3.7 angstrom Deposited 2019-01-15 | Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 42-meric（42） Consistent with all polymers |
Chain F
1–278（278 aa）
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 7 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |