Current Protein Identity:P32911
New Search
Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 2OGH Solution structure of yeast eIF1 Deposited 2007-01-05 | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
1–108(108 aa)
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7.2;298 K;Ionic strength (raw mmCIF value) 300mM NaCl;Pressure 1
NMR sample composition
50mM sodium phosphate, 300mM NaCl, 5mM bME, pH 7.2, 95% H2O, 5% D2O | 95% H2O/5% D2O
|
Resolution not provided |
| 2RVH NMR structure of eIF1 Deposited 2015-10-16 | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
1–108(108 aa)
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7;298 K;Ionic strength (raw mmCIF value) 170;Pressure ambient
NMR sample composition
0.4 mM [U-100% 13C; U-100% 15N] entity-1, 20 mM sodium phosphate-2, 150 mM sodium chloride-3, 1 mM DTT-4, 95% H2O/5% D2O | 95% H2O/5% D2O
|
Resolution not provided |
| 3J80 CryoEM structure of 40S-eIF1-eIF1A preinitiation complex Deposited 2014-08-28 | Assembly 1 Protein–RNA Heteromer;Protein × 36 PDB declaration: 37-meric(37) Consistent with all polymers |
Chain j
1–108(108 aa)
|
Not recorded | MG MAGNESIUM ION × 67 ZN ZINC ION × 3 | ELECTRON MICROSCOPY |
cryo-EM buffer
20 mM MES-KOH, 40 mM potassium acetate, 10 mM ammonium acetate, 8 mM magnesium acetate, 2 mM DTT;pH 6.5;20 mM MES-KOH, 40 mM potassium acetate, 10 mM ammonium acetate, 8 mM magnesium acetate, 2 mM DTT
cryo-EM vitrification conditions
Blot for 2.5 seconds before plunging;120 K;Cryogen ETHANE;Blot for 2.5 seconds before plunging into liquid ethane (FEI VITROBOT MARK I)
|
Resolution 3.75 Å |
| 3J81 CryoEM structure of a partial yeast 48S preinitiation complex Deposited 2014-08-29 | Assembly 1 Protein–RNA Heteromer;Protein × 39 PDB declaration: 42-meric(42) Consistent with all polymers |
Chain m
1–108(108 aa)
|
Mutation:S264Y | MG MAGNESIUM ION × 81 ZN ZINC ION × 3 MET METHIONINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
20 mM MES-KOH, 40 mM potassium acetate, 10 mM ammonium acetate, 8 mM magnesium acetate, 2 mM DTT;pH 6.5;20 mM MES-KOH, 40 mM potassium acetate, 10 mM ammonium acetate, 8 mM magnesium acetate, 2 mM DTT
cryo-EM vitrification conditions
Blot for 2.5 seconds before plunging;120 K;Cryogen ETHANE;Blot for 2.5 seconds before plunging into liquid ethane (FEI VITROBOT MARK I)
|
Resolution 4.00 Å |
| 3JAM CryoEM structure of 40S-eIF1A-eIF1 complex from yeast Deposited 2015-06-17 | Assembly 1 Protein–RNA Heteromer;Protein × 36 PDB declaration: 37-meric(37) Consistent with all polymers |
Chain j
1–108(108 aa)
|
Not recorded | MG MAGNESIUM ION × 80 ZN ZINC ION × 3 | ELECTRON MICROSCOPY |
cryo-EM buffer
20 mM MES-KOH, 40 mM potassium acetate, 10 mM ammonium acetate, 8 mM magnesium acetate, 2 mM DTT;pH 6.5;20 mM MES-KOH, 40 mM potassium acetate, 10 mM ammonium acetate, 8 mM magnesium acetate, 2 mM DTT
cryo-EM vitrification conditions
Blot for 2.5 to 3 seconds before plunging;120 K;Cryogen ETHANE;Blot for 2.5 to 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK I).
|
Resolution 3.46 Å |
| 3JAP Structure of a partial yeast 48S preinitiation complex in closed conformation Deposited 2015-06-18 | Assembly 1 Protein–RNA Heteromer;Protein × 44 PDB declaration: 47-meric(47) Consistent with all polymers |
Chain m
1–108(108 aa)
|
Not recorded | MG MAGNESIUM ION × 81 ZN ZINC ION × 4 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
20 mM MES-KOH, 40 mM potassium acetate, 10 mM ammonium acetate, 8 mM magnesium acetate, 2 mM DTT;pH 6.5;20 mM MES-KOH, 40 mM potassium acetate, 10 mM ammonium acetate, 8 mM magnesium acetate, 2 mM DTT
cryo-EM vitrification conditions
Blot for 2.5 to 3 seconds before plunging;120 K;Cryogen ETHANE;Blot for 2.5 to 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK I).
|
Resolution 4.90 Å |
| 6GSM Structure of a partial yeast 48S preinitiation complex in open conformation. Deposited 2018-06-14 | Assembly 1 Protein–RNA Heteromer;Protein × 44 PDB declaration: 47-meric(47) Consistent with all polymers |
Chain m
13–108(96 aa)
|
Not recorded | 7NO [(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-2-(phosphonooxymethyl)oxolan-3-yl] (2~{S})-2-azanyl-4-methylsulfanyl-butanoate × 1 MG MAGNESIUM ION × 82 ZN ZINC ION × 4 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.15 Å |
| 6GSN Structure of a partial yeast 48S preinitiation complex in closed conformation Deposited 2018-06-14 | Assembly 1 Protein–RNA Heteromer;Protein × 44 PDB declaration: 47-meric(47) Consistent with all polymers |
Chain m
19–108(90 aa)
|
Not recorded | MG MAGNESIUM ION × 81 ZN ZINC ION × 4 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MET METHIONINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.75 Å |
| 6ZCE Structure of a yeast ABCE1-bound 43S pre-initiation complex Deposited 2020-06-10 | Assembly 1 Protein–RNA Heteromer;Protein × 44 PDB declaration: 45-meric(45) Consistent with all polymers |
Chain m
1–108(108 aa)
|
Not recorded | ZN ZINC ION × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.30 Å |
| 8S8F Structure of a yeast 48S-AUC preinitiation complex in closed conformation (model py48S-AUC-3.2) Deposited 2024-03-06 | Assembly 1 Protein–RNA Heteromer;Protein × 39 PDB declaration: 42-meric(42) Consistent with all polymers |
Chain m
1–108(108 aa)
|
Not recorded | MG MAGNESIUM ION × 116 ZN ZINC ION × 4 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MET METHIONINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.95 Å |
| 8S8I Structure of a yeast 48S-AUC preinitiation complex in closed conformation (model py48S-AUC-eIF1) Deposited 2024-03-06 | Assembly 1 Protein–RNA Heteromer;Protein × 39 PDB declaration: 42-meric(42) Consistent with all polymers |
Chain m
1–108(108 aa)
|
Not recorded | MG MAGNESIUM ION × 117 ZN ZINC ION × 4 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MET METHIONINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.30 Å |
| 8S8K Structure of a yeast 48S-AUC preinitiation complex in swivelled conformation (model py48S-AUC-swiv-eIF1) Deposited 2024-03-06 | Assembly 1 Protein–RNA Heteromer;Protein × 40 PDB declaration: 43-meric(43) Consistent with all polymers |
Chain m
1–108(108 aa)
|
Not recorded | MG MAGNESIUM ION × 96 ZN ZINC ION × 4 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MET METHIONINE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.00 Å |