Current Protein Identity:P40217
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 3JAP Structure of a partial yeast 48S preinitiation complex in closed conformation Deposited 2015-06-18 | Assembly 1 Protein–RNA Heteromer;Protein × 44 PDB declaration: 47-meric(47) Consistent with all polymers |
Chain q
1–347(347 aa)
|
Not recorded | MG MAGNESIUM ION × 81 ZN ZINC ION × 4 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
20 mM MES-KOH, 40 mM potassium acetate, 10 mM ammonium acetate, 8 mM magnesium acetate, 2 mM DTT;pH 6.5;20 mM MES-KOH, 40 mM potassium acetate, 10 mM ammonium acetate, 8 mM magnesium acetate, 2 mM DTT
cryo-EM vitrification conditions
Blot for 2.5 to 3 seconds before plunging;120 K;Cryogen ETHANE;Blot for 2.5 to 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK I).
|
Resolution 4.90 Å |
| 3ZWL Structure of eukaryotic translation initiation factor eIF3i complex with eIF3b C-terminus (655-700) Deposited 2011-08-01 | Assembly 1 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain B
1–347(347 aa)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8.8;0.1 M TRIS PH 8.8, 0.1 M LI2SO4, 30% PEG4000, 8% DIAMINOPENTANE
|
Resolution 2.20 Å R-free 0.247 |
| 3ZWL Structure of eukaryotic translation initiation factor eIF3i complex with eIF3b C-terminus (655-700) Deposited 2011-08-01 | Assembly 2 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain D
1–347(347 aa)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8.8;0.1 M TRIS PH 8.8, 0.1 M LI2SO4, 30% PEG4000, 8% DIAMINOPENTANE
|
Resolution 2.20 Å R-free 0.247 |
| 5A5U Structure of mammalian eIF3 in the context of the 43S preinitiation complex Deposited 2015-06-21 | Assembly 1 Protein heterocomplex Heteromer;Protein × 3 PDB declaration: trimeric(3) Consistent with protein count |
Chain I
1–347(347 aa)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM vitrification conditions
Cryogen ETHANE;VITRIFICATION 1 -- CRYOGEN- ETHANE, HUMIDITY- 100, TEMPERATURE- 120, INSTRUMENT- FEI VITROBOT MARK IV,
|
Resolution 9.00 Å |
| 6FYX Structure of a partial yeast 48S preinitiation complex with eIF5 N-terminal domain (model C1) Deposited 2018-03-12 | Assembly 1 Protein–RNA Heteromer;Protein × 44 PDB declaration: 47-meric(47) Consistent with all polymers |
Chain s
1–347(347 aa)
|
Not recorded | MG MAGNESIUM ION × 117 ZN ZINC ION × 5 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 6GSM Structure of a partial yeast 48S preinitiation complex in open conformation. Deposited 2018-06-14 | Assembly 1 Protein–RNA Heteromer;Protein × 44 PDB declaration: 47-meric(47) Consistent with all polymers |
Chain s
1–342(342 aa)
|
Not recorded | 7NO [(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-2-(phosphonooxymethyl)oxolan-3-yl] (2~{S})-2-azanyl-4-methylsulfanyl-butanoate × 1 MG MAGNESIUM ION × 82 ZN ZINC ION × 4 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.15 Å |
| 6ZCE Structure of a yeast ABCE1-bound 43S pre-initiation complex Deposited 2020-06-10 | Assembly 1 Protein–RNA Heteromer;Protein × 44 PDB declaration: 45-meric(45) Consistent with all polymers |
Chain l
1–347(347 aa)
|
Not recorded | ZN ZINC ION × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.30 Å |
| 6ZU9 Structure of a yeast ABCE1-bound 48S initiation complex Deposited 2020-07-22 | Assembly 1 Protein–RNA Heteromer;Protein × 41 PDB declaration: 44-meric(44) Consistent with all polymers |
Chain l
1–347(347 aa)
|
Not recorded | MG MAGNESIUM ION × 4 ZN ZINC ION × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.20 Å |