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Protein Structures and Oligomeric States

Enter a protein name, UniProt accession or PDB ID. Name searches ask you to confirm the protein identity before related structures are queried.

Current Protein Identity：P54578 New Search
23PDB entries
29Biological assembly
39chain/assembly records
Main Difference Dimensions in This Set
Different construct Different mutation/modification Different assembly state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics

Difference tags compare only the current result set; every original PDB and assembly record remains separate.

Related-Structure Differences

Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.

PDB Entry Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Experimental Method Experimental Conditions Structure Quality
2AYN Structure of USP14, a proteasome-associated deubiquitinating enzyme Deposited 2005-09-07 Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count
Chain A 90–493（404 aa）
Chain B 90–493（404 aa）
Chain C 90–493（404 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；290 K；MES, ammonium sulfate, PEG monomethyl ether, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K
Resolution 3.20 Å R-free 0.322
2AYO Structure of USP14 bound to ubquitin aldehyde Deposited 2005-09-07 Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 90–493（404 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；290 K；Tris, calsium chloride, PEG1000, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K
Resolution 3.50 Å R-free 0.330
2AYO Structure of USP14 bound to ubquitin aldehyde Deposited 2005-09-07 Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count
Chain A 90–493（404 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；290 K；Tris, calsium chloride, PEG1000, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K
Resolution 3.50 Å R-free 0.330
5GJQ Structure of the human 26S proteasome bound to USP14-UbAl Deposited 2016-07-01 Assembly 1 Protein heterocomplex Heteromer；Protein × 48 PDB declaration: 48-meric（48） Consistent with protein count
Chain x 1–494（494 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；blot for 2 seconds before plunging
Resolution 4.35 Å
6IIK USP14 catalytic domain with IU1 Deposited 2018-10-06 Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 96–494（399 aa）
Chain B 96–494（399 aa）
Not recorded IU1 1-[1-(4-fluorophenyl)-2,5-dimethyl-1H-pyrrol-3-yl]-2-(pyrrolidin-1-yl)ethan-1-one × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；277 K；NH4F. PEG 3350, glycine, Cscl
Resolution 1.97 Å R-free 0.218
6IIL USP14 catalytic domain bind to IU1-47 Deposited 2018-10-07 Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 96–494（399 aa） Fragment：catalytic domain
Chain B 96–494（399 aa） Fragment：catalytic domain
Not recorded A8F 1-[1-(4-chlorophenyl)-2,5-dimethyl-1H-pyrrol-3-yl]-2-(piperidin-1-yl)ethan-1-one × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；277 K；PEG 3350, NH4F, glycine, Cscl
Resolution 2.20 Å R-free 0.232
6IIM USP14 catalytic domain with IU1-206 Deposited 2018-10-07 Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 96–494（399 aa） Fragment：catalytic domain
Chain B 96–494（399 aa） Fragment：catalytic domain
Not recorded A8L 1-[1-(4-chlorophenyl)-2,5-dimethyl-1H-pyrrol-3-yl]-2-(4-hydroxypiperidin-1-yl)ethan-1-one × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；277 K；PEG 3350, NH4F, Cscl, glycine
Resolution 2.21 Å R-free 0.247
6IIN USP14 catalytic domain with IU1-248 Deposited 2018-10-07 Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 101–485（385 aa） Fragment：catalytic domain
Chain B 101–485（385 aa） Fragment：catalytic domain
Not recorded A8O 4-{3-[(4-hydroxypiperidin-1-yl)acetyl]-2,5-dimethyl-1H-pyrrol-1-yl}benzonitrile × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；277 K；PEG 3350, NH4F, Cscl, glycine
Resolution 2.53 Å R-free 0.264
6LVS USP14 catalytic domain mutant C114S Deposited 2020-02-04 Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain A 92–494（403 aa） Fragment：catalytic domain
Mutation：C114S, deletion of 223-231 GOL GLYCEROL × 3 NA SODIUM ION × 7 FMT FORMIC ACID × 2 BME BETA-MERCAPTOETHANOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；295.15 K；0.2M HcooNa, 0.1M BICINE, 20% PEGmme 5000
Resolution 2.73 Å R-free 0.277
6LVS USP14 catalytic domain mutant C114S Deposited 2020-02-04 Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain B 92–494（403 aa） Fragment：catalytic domain
Mutation：C114S, deletion of 223-231 GOL GLYCEROL × 3 NA SODIUM ION × 8 FMT FORMIC ACID × 7 BME BETA-MERCAPTOETHANOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；295.15 K；0.2M HcooNa, 0.1M BICINE, 20% PEGmme 5000
Resolution 2.73 Å R-free 0.277
6LVS USP14 catalytic domain mutant C114S Deposited 2020-02-04 Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain C 92–494（403 aa） Fragment：catalytic domain
Mutation：C114S, deletion of 223-231 GOL GLYCEROL × 2 NA SODIUM ION × 8 FMT FORMIC ACID × 6 BME BETA-MERCAPTOETHANOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；295.15 K；0.2M HcooNa, 0.1M BICINE, 20% PEGmme 5000
Resolution 2.73 Å R-free 0.277
6LVS USP14 catalytic domain mutant C114S Deposited 2020-02-04 Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain D 92–494（403 aa） Fragment：catalytic domain
Mutation：C114S, deletion of 223-231 GOL GLYCEROL × 2 NA SODIUM ION × 5 FMT FORMIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；295.15 K；0.2M HcooNa, 0.1M BICINE, 20% PEGmme 5000
Resolution 2.73 Å R-free 0.277
6LVS USP14 catalytic domain mutant C114S Deposited 2020-02-04 Assembly 5 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain E 92–494（403 aa） Fragment：catalytic domain
Mutation：C114S, deletion of 223-231 GOL GLYCEROL × 2 NA SODIUM ION × 7 FMT FORMIC ACID × 5 BME BETA-MERCAPTOETHANOL × 3 EDO 1,2-ETHANEDIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；295.15 K；0.2M HcooNa, 0.1M BICINE, 20% PEGmme 5000
Resolution 2.73 Å R-free 0.277
6LVS USP14 catalytic domain mutant C114S Deposited 2020-02-04 Assembly 6 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count
Chain F 92–494（403 aa） Fragment：catalytic domain
Mutation：C114S, deletion of 223-231 NA SODIUM ION × 6 FMT FORMIC ACID × 4 BME BETA-MERCAPTOETHANOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；295.15 K；0.2M HcooNa, 0.1M BICINE, 20% PEGmme 5000
Resolution 2.73 Å R-free 0.277
7W37 Structure of USP14-bound human 26S proteasome in state EA1_UBL Deposited 2021-11-25 Assembly 1 Protein heterocomplex Heteromer；Protein × 47 PDB declaration: 47-meric（47） Consistent with protein count
Chain x 1–494（494 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
7W38 Structure of USP14-bound human 26S proteasome in state EA2.0_UBL Deposited 2021-11-25 Assembly 1 Protein heterocomplex Heteromer；Protein × 48 PDB declaration: 48-meric（48） Consistent with protein count
Chain x 1–494（494 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 6 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7W39 Structure of USP14-bound human 26S proteasome in state EA2.1_UBL Deposited 2021-11-25 Assembly 1 Protein heterocomplex Heteromer；Protein × 49 PDB declaration: 49-meric（49） Consistent with protein count
Chain x 1–494（494 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 5 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
7W3A Structure of USP14-bound human 26S proteasome in substrate-engaged state ED4_USP14 Deposited 2021-11-25 Assembly 1 Protein heterocomplex Heteromer；Protein × 49 PDB declaration: 49-meric（49） Consistent with protein count
Chain x 1–494（494 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 4 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
7W3B Structure of USP14-bound human 26S proteasome in substrate-engaged state ED5_USP14 Deposited 2021-11-25 Assembly 1 Protein heterocomplex Heteromer；Protein × 49 PDB declaration: 49-meric（49） Consistent with protein count
Chain x 1–494（494 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7W3C Structure of USP14-bound human 26S proteasome in substrate-engaged state ED0_USP14 Deposited 2021-11-25 Assembly 1 Protein heterocomplex Heteromer；Protein × 49 PDB declaration: 49-meric（49） Consistent with protein count
Chain x 1–494（494 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
7W3F Structure of USP14-bound human 26S proteasome in substrate-engaged state ED1_USP14 Deposited 2021-11-25 Assembly 1 Protein heterocomplex Heteromer；Protein × 49 PDB declaration: 49-meric（49） Consistent with protein count
Chain x 1–494（494 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7W3G Structure of USP14-bound human 26S proteasome in substrate-engaged state ED2.0_USP14 Deposited 2021-11-25 Assembly 1 Protein heterocomplex Heteromer；Protein × 49 PDB declaration: 49-meric（49） Consistent with protein count
Chain x 1–494（494 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
7W3H Structure of USP14-bound human 26S proteasome in substrate-engaged state ED2.1_USP14 Deposited 2021-11-25 Assembly 1 Protein heterocomplex Heteromer；Protein × 49 PDB declaration: 49-meric（49） Consistent with protein count
Chain x 1–494（494 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 4 MG MAGNESIUM ION × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
7W3I Structure of USP14-bound human 26S proteasome in substrate-inhibited state SB_USP14 Deposited 2021-11-25 Assembly 1 Protein heterocomplex Heteromer；Protein × 48 PDB declaration: 48-meric（48） Consistent with protein count
Chain x 1–494（494 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
7W3J Structure of USP14-bound human 26S proteasome in substrate-inhibited state SC_USP14 Deposited 2021-11-25 Assembly 1 Protein heterocomplex Heteromer；Protein × 48 PDB declaration: 48-meric（48） Consistent with protein count
Chain x 1–494（494 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 4 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.50 Å
7W3K Structure of USP14-bound human 26S proteasome in substrate-inhibited state SD4_USP14 Deposited 2021-11-25 Assembly 1 Protein heterocomplex Heteromer；Protein × 48 PDB declaration: 48-meric（48） Consistent with protein count
Chain x 1–494（494 aa）
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7W3M Structure of USP14-bound human 26S proteasome in substrate-inhibited state SD5_USP14 Deposited 2021-11-25 Assembly 1 Protein heterocomplex Heteromer；Protein × 48 PDB declaration: 48-meric（48） Consistent with protein count
Chain x 1–494（494 aa）
Not recorded ATP ADENOSINE-5'-TRIPHOSPHATE × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
9F19 Human USP30 chimera in complex with NK036 inhibitor Deposited 2024-04-18 Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 248–272（25 aa）
Chain A 295–320（26 aa）
Chain B 248–272（25 aa）
Chain B 295–320（26 aa）
Not recorded A1H8X 4-fluoranyl-~{N}-[(2~{S})-1-[[4-[(2-methyl-1-oxidanyl-propan-2-yl)sulfamoyl]phenyl]amino]-1-oxidanylidene-3-phenyl-propan-2-yl]benzamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；76 mM NaOH, 100 mM Bicine, 10.2% (w/v) PEG 20,000, 1% (v/v) Dioxane, 10 mM L-Proline (Crystal 1)
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；85 mM NaOH, 100 mM Bicine, 10.2% (w/v) PEG 20,000, 1% (v/v) Dioxane, 10 mM L-Proline (Crystal 2)
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；88 mM NaOH, 100 mM Bicine, 11% (w/v) PEG 20,000, 1% (v/v) Dioxane, 10 mM Sarcosine (Crystal 3)
Resolution 2.75 Å R-free 0.266
9F6G Human USP30 chimera bound to Ubiquitin-PA Deposited 2024-05-01 Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count
Chain A 248–272（25 aa）
Chain A 295–320（26 aa）
Not recorded AYE prop-2-en-1-amine × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；0.56 M sodium citrate pH 7.0
Resolution 1.50 Å R-free 0.211

This page presents structural evidence recorded in PDB/mmCIF.“No recorded non-water small molecule” does not prove that the experimental sample was ligand-free; experimental conditions belong to the PDB experiment, while ligands and copy counts are shown for each biological assembly.