Current Protein Identity：P60174
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1HTI CRYSTAL STRUCTURE OF RECOMBINANT HUMAN TRIOSEPHOSPHATE ISOMERASE AT 2.8 ANGSTROMS RESOLUTION. TRIOSEPHOSPHATE ISOMERASE RELATED HUMAN GENETIC DISORDERS AND COMPARISON WITH THE TRYPANOSOMAL ENZYME Deposited 1994-10-12 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–248（248 aa）
Chain B
1–248（248 aa）
|
Not recorded | PGA 2-PHOSPHOGLYCOLIC ACID × 1 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.80 Å |
| 1WYI human triosephosphate isomerase of new crystal form Deposited 2005-02-14 | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–248（248 aa）
Chain B
1–248（248 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
gel-tube under microgavity；pH 8；293 K；PEG4000, MgCl2, pH 8.0, gel-tube under microgavity, temperature 293K
|
Resolution 2.20 Å R-free 0.296 |
| 1WYI human triosephosphate isomerase of new crystal form Deposited 2005-02-14 | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–248（248 aa）
Chain B
1–248（248 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
gel-tube under microgavity；pH 8；293 K；PEG4000, MgCl2, pH 8.0, gel-tube under microgavity, temperature 293K
|
Resolution 2.20 Å R-free 0.296 |
| 2IAM Structural basis for recognition of mutant self by a tumor-specific, MHC class II-restricted TCR Deposited 2006-09-08 | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain P
22–36（15 aa）
Fragment：residues 23-37 (22-36)
|
Mutation：T28I | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；295 K；8% PEG6000, 0.1M di-ammonium phosphate, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 2.80 Å R-free 0.279 |
| 2IAN Structural basis for recognition of mutant self by a tumor-specific, MHC class II-restricted TCR Deposited 2006-09-08 | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain C
22–36（15 aa）
Fragment：residues 23-37 (22-36)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；295 K；10% PEG6000, 0.1M di-ammonium phosphate, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 2.80 Å R-free 0.296 |
| 2IAN Structural basis for recognition of mutant self by a tumor-specific, MHC class II-restricted TCR Deposited 2006-09-08 | Assembly 2 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain H
22–36（15 aa）
Fragment：residues 23-37 (22-36)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；295 K；10% PEG6000, 0.1M di-ammonium phosphate, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 2.80 Å R-free 0.296 |
| 2IAN Structural basis for recognition of mutant self by a tumor-specific, MHC class II-restricted TCR Deposited 2006-09-08 | Assembly 3 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain M
22–36（15 aa）
Fragment：residues 23-37 (22-36)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；295 K；10% PEG6000, 0.1M di-ammonium phosphate, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 2.80 Å R-free 0.296 |
| 2IAN Structural basis for recognition of mutant self by a tumor-specific, MHC class II-restricted TCR Deposited 2006-09-08 | Assembly 4 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain R
22–36（15 aa）
Fragment：residues 23-37 (22-36)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；295 K；10% PEG6000, 0.1M di-ammonium phosphate, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 2.80 Å R-free 0.296 |
| 2JK2 STRUCTURAL BASIS OF HUMAN TRIOSEPHOSPHATE ISOMERASE DEFICIENCY. CRYSTAL STRUCTURE OF THE WILD TYPE ENZYME. Deposited 2008-06-22 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
2–249（248 aa）
Fragment：RESIDUES 2-249
Chain B
2–249（248 aa）
Fragment：RESIDUES 2-249
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8.5；100 MM TRIS PH 8.5, 20% PEG MME2000, 10 MM NICL2
|
Resolution 1.70 Å R-free 0.252 |
| 2VOM Structural basis of human triosephosphate isomerase deficiency. Mutation E104D and correlation to solvent perturbation. Deposited 2008-02-19 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
2–249（248 aa）
Fragment：RESIDUES 2-249
Chain B
2–249（248 aa）
Fragment：RESIDUES 2-249
|
Mutation：YES Mutation：YES | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8.5；100 MM TRIS PH 8.5, 20% PEG MME2000, 4% POLYPROPYLENE GLYCOL P400, 10 MM NICL2
|
Resolution 1.85 Å R-free 0.253 |
| 2VOM Structural basis of human triosephosphate isomerase deficiency. Mutation E104D and correlation to solvent perturbation. Deposited 2008-02-19 | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
2–249（248 aa）
Fragment：RESIDUES 2-249
Chain D
2–249（248 aa）
Fragment：RESIDUES 2-249
|
Mutation：YES Mutation：YES | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8.5；100 MM TRIS PH 8.5, 20% PEG MME2000, 4% POLYPROPYLENE GLYCOL P400, 10 MM NICL2
|
Resolution 1.85 Å R-free 0.253 |
| 4BR1 Protease-induced heterodimer of human triosephosphate isomerase. Deposited 2013-06-03 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
41–286（246 aa）
Chain B
41–286（246 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8.5；100 MM TRIS PH 8.5, 30% POLYETHYLENE GLYCOL 4000, 200 MM SODIUM ACETATE TRIHYDRATE
|
Resolution 1.90 Å R-free 0.217 |
| 4E41 Structural basis for the recognition of mutant self by a tumor-specific, MHC class II-restricted T cell receptor G4 Deposited 2012-03-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain C
60–74（15 aa）
Fragment：unp residues 60-74
|
Not recorded | NA SODIUM ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 8；298 K；20% (wt/vol) polyethylene glycol 1000, 0.2 M calcium acetate, 0.1 M imidazole, pH 8.0, VAPOR DIFFUSION, temperature 298K
|
Resolution 2.60 Å R-free 0.264 |
| 4E41 Structural basis for the recognition of mutant self by a tumor-specific, MHC class II-restricted T cell receptor G4 Deposited 2012-03-11 | Assembly 2 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain H
60–74（15 aa）
Fragment：unp residues 60-74
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 8；298 K；20% (wt/vol) polyethylene glycol 1000, 0.2 M calcium acetate, 0.1 M imidazole, pH 8.0, VAPOR DIFFUSION, temperature 298K
|
Resolution 2.60 Å R-free 0.264 |
| 4POC Structure of Triosephosphate Isomerase Wild Type human enzyme. Deposited 2014-02-25 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
38–286（249 aa）
Chain B
38–286（249 aa）
|
Not recorded | K POTASSIUM ION × 2 NA SODIUM ION × 1 BR BROMIDE ION × 2 PO4 PHOSPHATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；277 K；35% PEG 2000 MME, 0.05 KBr, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 1.60 Å R-free 0.187 |
| 4POD Structure of Triosephosphate Isomerase I170V mutant human enzyme. Deposited 2014-02-25 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
38–286（249 aa）
Chain B
38–286（249 aa）
|
Mutation：I170V Mutation：I170V | K POTASSIUM ION × 2 NA SODIUM ION × 1 PO4 PHOSPHATE ION × 1 BR BROMIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；277 K；34% PEG 2000 MME, 0.05 KBr, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 1.99 Å R-free 0.213 |
| 4UNK Crystal structure of human triosephosphate isomerase (mutant N15D) Deposited 2014-05-29 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
39–286（248 aa）
Fragment：RESIDUES 39-286
Chain B
39–286（248 aa）
Fragment：RESIDUES 39-286
|
Mutation：YES Mutation：YES | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8.5；200 MM AMMONIUM ACETATE, 100 MM TRIS PH 8.5, 25% V/VW/V POLYETHYLENE GLYCOL 3350
|
Resolution 2.00 Å R-free 0.224 |
| 4UNL Crystal structure of a single mutant (N71D) of triosephosphate isomerase from human Deposited 2014-05-29 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
39–286（248 aa）
Fragment：RESIDUES 39-286
Chain B
39–286（248 aa）
Fragment：RESIDUES 39-286
|
Mutation：YES Mutation：YES | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 5.6；PROTEIN WAS CRYSTALLIZED FROM: 0.2 M AMMONIUM ACETATE, 0.1 M SODIUM CITRATE TRIBASIC DIHYDRATE PH 5.6, 30% W/V POLYETHYLENE GLYCOL 4,000
|
Resolution 1.50 Å R-free 0.226 |
| 4ZVJ Structure of human triose phosphate isomerase K13M Deposited 2015-05-18 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
38–286（249 aa）
Chain B
38–286（249 aa）
|
Mutation：K13M Mutation：K13M | NA SODIUM ION × 1 K POTASSIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；277 K；35% PEG 2000 MME, 50 mM KBr, Tris pH 7.5
|
Resolution 1.70 Å R-free 0.187 |
| 6C2G Human triosephosphate isomerase mutant V231M Deposited 2018-01-08 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
38–286（249 aa）
Chain C
38–286（249 aa）
|
Mutation：V231M Mutation：V231M | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；293.15 K；0.2 M Magnesium chloride hexahydrate, 0.1 M TRIS hydrochloride pH 8.5, 30% w/v Polyethylene glycol 4,000.
|
Resolution 2.30 Å R-free 0.226 |
| 6C2G Human triosephosphate isomerase mutant V231M Deposited 2018-01-08 | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
38–286（249 aa）
Chain D
38–286（249 aa）
|
Mutation：V231M Mutation：V231M | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；293.15 K；0.2 M Magnesium chloride hexahydrate, 0.1 M TRIS hydrochloride pH 8.5, 30% w/v Polyethylene glycol 4,000.
|
Resolution 2.30 Å R-free 0.226 |
| 6D43 CHARACTERIZATION OF HUMAN TRIOSEPHOSPHATE ISOMERASE S-NITROSYLATION Deposited 2018-04-17 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
41–286（246 aa）
Chain B
41–286（246 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | IPA ISOPROPYL ALCOHOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；283 K；0.1 M HEPES pH 7.5, 20% PEG 4000, and 10% 2-propanol
|
Resolution 2.04 Å R-free 0.256 |
| 6NLH Structure of human triose phosphate isomerase R189A Deposited 2019-01-08 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
42–286（245 aa）
Chain E
42–286（245 aa）
|
Mutation：R189A Mutation：R189A | NA SODIUM ION × 4 BR BROMIDE ION × 1 PO4 PHOSPHATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；277 K；31% PEG 3350, 50 mM Potassium Bromide, Tris pH7.5
|
Resolution 2.20 Å R-free 0.216 |
| 6NLH Structure of human triose phosphate isomerase R189A Deposited 2019-01-08 | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
42–286（245 aa）
Chain C
42–286（245 aa）
|
Mutation：R189A Mutation：R189A | NA SODIUM ION × 7 BR BROMIDE ION × 1 PO4 PHOSPHATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；277 K；31% PEG 3350, 50 mM Potassium Bromide, Tris pH7.5
|
Resolution 2.20 Å R-free 0.216 |
| 6NLH Structure of human triose phosphate isomerase R189A Deposited 2019-01-08 | Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain D
42–286（245 aa）
Chain F
42–286（245 aa）
|
Mutation：R189A Mutation：R189A | NA SODIUM ION × 5 BR BROMIDE ION × 1 PO4 PHOSPHATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；277 K；31% PEG 3350, 50 mM Potassium Bromide, Tris pH7.5
|
Resolution 2.20 Å R-free 0.216 |
| 6NLH Structure of human triose phosphate isomerase R189A Deposited 2019-01-08 | Assembly 4 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain G
42–286（245 aa）
Chain H
42–286（245 aa）
|
Mutation：R189A Mutation：R189A | NA SODIUM ION × 4 BR BROMIDE ION × 1 PO4 PHOSPHATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；277 K；31% PEG 3350, 50 mM Potassium Bromide, Tris pH7.5
|
Resolution 2.20 Å R-free 0.216 |
| 6UP1 Triosephosphate isomerase deficiency: Effect of F240L mutation on enzyme structure Deposited 2019-10-16 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
39–286（248 aa）
Chain B
39–286（248 aa）
|
Not recorded | IPA ISOPROPYL ALCOHOL × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；283.15 K；0.1 M HEPES pH 7.5, 20% PEG 4000, and 10% 2-propanol
|
Resolution 1.83 Å R-free 0.261 |
| 6UP5 Triosephosphate isomerase deficiency: Effect of F240L mutation on enzyme structure Deposited 2019-10-16 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
39–286（248 aa）
Chain B
39–286（248 aa）
|
Not recorded | GOL GLYCEROL × 3 IPA ISOPROPYL ALCOHOL × 3 PGA 2-PHOSPHOGLYCOLIC ACID × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；283 K；0.1 M HEPES pH 7.5, 20% PEG 4000, and 10% 2-propanol
|
Resolution 1.92 Å R-free 0.232 |
| 6UP8 Triosephosphate isomerase deficiency: Effect of F240L mutation on enzyme structure Deposited 2019-10-16 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
39–286（248 aa）
Chain B
39–286（248 aa）
|
Mutation：F240L Mutation：F240L | IPA ISOPROPYL ALCOHOL × 5 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；283 K；0.1 M HEPES pH 7.5, 20% PEG 4000, and 10% 2-propanol
|
Resolution 2.00 Å R-free 0.253 |
| 6UPF Triosephosphate isomerase deficiency: Effect of F240L mutation on enzyme structure Deposited 2019-10-17 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
39–286（248 aa）
Chain B
39–286（248 aa）
|
Mutation：F240L Mutation：F240L | PGA 2-PHOSPHOGLYCOLIC ACID × 2 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；283 K；0.05 M Tris-HCl pH 7.5, 0.05 M NaCl, 1 mM EDTA, 15% PEG 4000 and 2 mM 2-PG
|
Resolution 1.65 Å R-free 0.197 |
| 7RDE Human Triose Phosphate Isomerase Q181P Deposited 2021-07-09 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–249（249 aa）
Chain B
1–249（249 aa）
|
Mutation：Q181P Mutation：Q181P | BR BROMIDE ION × 2 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；277 K；30% PEG 3350, 50mM Potassium Bromide, Tris pH7.5
|
Resolution 1.31 Å R-free 0.162 |
| 7SX1 human triosephosphate isomerase mutant v154m Deposited 2021-11-22 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–249（249 aa）
Chain B
1–249（249 aa）
|
Mutation：V154M Mutation：V154M | IPA ISOPROPYL ALCOHOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；283 K；0.1 M HEPES pH 7.5, 20% PEG 4000, and 10% 2-propanol
|
Resolution 2.23 Å R-free 0.225 |
| 7T0Q human triosephosphate isomerase mutant v154m Deposited 2021-11-30 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
2–249（248 aa）
Chain B
2–249（248 aa）
|
Mutation：V154M Mutation：V154M | PGA 2-PHOSPHOGLYCOLIC ACID × 1 GOL GLYCEROL × 2 IPA ISOPROPYL ALCOHOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；283 K；0.1 M HEPES pH 7.5, 20% PEG 4000, and 10% 2-propanol
|
Resolution 2.00 Å R-free 0.206 |
| 7UXB Human triosephosphate isomerase mutant G122R Deposited 2022-05-05 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–249（249 aa）
Chain B
1–249（249 aa）
|
Mutation：G122R Mutation：G122R | IPA ISOPROPYL ALCOHOL × 1 GOL GLYCEROL × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；283 K；0.1 M HEPES pH 7.5, 20% PEG 4000, and 10% 2-propanol
|
Resolution 2.00 Å R-free 0.228 |
| 7UXV human triosephosphate isomerase mutant G122R Deposited 2022-05-06 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–249（249 aa）
Chain B
1–249（249 aa）
|
Mutation：G122R Mutation：G122R | PGA 2-PHOSPHOGLYCOLIC ACID × 2 GOL GLYCEROL × 4 IPA ISOPROPYL ALCOHOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；283 K；0.1 M HEPES pH 7.5, 20% PEG 4000, and 10% 2-porpanol
|
Resolution 2.15 Å R-free 0.246 |
| 9F69 Crystal structure of human triose phosphate isomerase with methyl malonic acid ligand Deposited 2024-04-30 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
5–249（245 aa）
|
Not recorded | DXX METHYLMALONIC ACID × 2 BR BROMIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；295 K；Protein buffer: 20 mM Tris pH 7.4, 30 mM NaCl.
Reservoir: 0.14 M KBr, 24% PEG 2000 MME.
Hanging drop: 1.5:1.5:1.0 ul - Reservoir-Protein (8 mg/ml)-Seed stock.
Cryoprotectant = 20% glycerol;
Ligand soaking performed for 5-6 min in a mixture containing 24 mM methylmalonate (pH adjusted to 7.4), mother liquor, and cryo-protectant
Ligand soaking and cryoprotectant were performed simultaneously
|
Resolution 1.17 Å R-free 0.184 |
| 9FFC Crystal structure of human triose phosphate isomerase with glycerol-3-phosphate ligand Deposited 2024-05-23 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–249（249 aa）
|
Not recorded | G3P SN-GLYCEROL-3-PHOSPHATE × 2 BR BROMIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.4；295 K；Protein buffer: 20 mM Tris pH 7.4, 30 mM NaCl.
Reservoir: 0.14 M KBr, 24% PEG 2000 MME.
Hanging drop: 1.5:1.5:1.0 ul - Reservoir-Protein (8 mg/ml)-Seed stock.
Cryoprotectant = 20% glycerol;
Ligand soaking performed for 5-6 min in a mixture containing 20 mM glycerol-3-phosphate (pH adjusted to 7.4), mother liquor, and cryo-protectant
Ligand soaking and cryoprotectant were performed simultaneously
|
Resolution 1.25 Å R-free 0.180 |