Current Protein Identity:Q07457
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 4R7E Structure of Bre1 RING domain Deposited 2014-08-27 | Assembly 1 Protein homooligomer Homooligomer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain A
632–700(69 aa)
Fragment:RING domain (UNP residues 632-700)
|
Not recorded | ZN ZINC ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 5.2;293 K;2.0 M ammonium sulfate and 0.1 mM sodium acetate, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.25 Å R-free 0.247 |
| 7UV8 Rad6-Bre1 Complex Deposited 2022-04-29 | Assembly 1 Protein heterocomplex Heteromer;Protein × 3 PDB declaration: trimeric(3) Consistent with protein count |
Chain U
1–212(212 aa)
Chain V
1–212(212 aa)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 5.6;286.15 K;0.05 to 0.1 M MMT buffer and 15-25% PEG400
|
Resolution 2.70 Å R-free 0.280 |
| 7UVC Rad6(P43L)-Bre1 Complex Deposited 2022-04-29 | Assembly 1 Protein heterocomplex Heteromer;Protein × 3 PDB declaration: trimeric(3) Consistent with protein count |
Chain U
1–212(212 aa)
Chain V
1–212(212 aa)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 5.6;286 K;0.05 to 0.1 M MMT buffer and 15-25% PEG400
|
Resolution 3.05 Å R-free 0.279 |
| 8IEG Bre1(mRBD-RING)/Rad6-Ub/nucleosome complex Deposited 2023-02-15 | Assembly 1 Protein–DNA Heteromer;Protein × 11 PDB declaration: tridecameric(13) Consistent with all polymers |
Chain A
637–700(64 aa)
Chain B
637–700(64 aa)
|
Not recorded | ZN ZINC ION × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.44 Å |
| 8T3T Structure of Bre1-nucleosome complex - state3 Deposited 2023-06-07 | Assembly 1 Protein–DNA Heteromer;Protein × 10 PDB declaration: dodecameric(12) Consistent with all polymers |
Chain K
591–700(110 aa)
Chain L
591–700(110 aa)
|
Not recorded | ZN ZINC ION × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5;20 mM HEPES pH 7.5, 50 mM NaCl, 1 mM DTT
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.21 Å |
| 8T3W Structure of Bre1-nucleosome complex - state2 Deposited 2023-06-07 | Assembly 1 Protein–DNA Heteromer;Protein × 10 PDB declaration: dodecameric(12) Consistent with all polymers |
Chain K
591–700(110 aa)
Chain L
591–700(110 aa)
|
Not recorded | ZN ZINC ION × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5;20 mM HEPES pH 7.5, 50 mM NaCl, 1 mM DTT
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.25 Å |
| 8T3Y Structure of Bre1-nucleosome complex - state1 Deposited 2023-06-08 | Assembly 1 Protein–DNA Heteromer;Protein × 10 PDB declaration: dodecameric(12) Consistent with all polymers |
Chain K
591–700(110 aa)
Chain L
591–700(110 aa)
|
Not recorded | ZN ZINC ION × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5;20 mM HEPES, pH 7.5, 50 mM NaCl, 1 mM DTT
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.47 Å |
| 9M3I Crystal structure of the Bre1-Lge1 complex Deposited 2025-03-02 | Assembly 1 Protein heterocomplex Heteromer;Protein × 5 PDB declaration: pentameric(5) Consistent with protein count |
Chain A
313–700(388 aa)
Chain B
313–700(388 aa)
Chain D
227–312(86 aa)
Chain E
227–312(86 aa)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 7;277 K;120 mM ammonium citrate (pH 7.0), 12 % polyethylene glycol monomethyl ether (molecular weight 5000 Da), 0.5% beta-mercaptoethanol and 20 mM dithiothreitol
|
Resolution 3.50 Å R-free 0.325 |