当前蛋白身份：Q12756
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相关结构差异明细
一行代表一个 PDB 条目中的一个 biological assembly；同一蛋白的多个单体会分别列出。
| PDB 条目 | Assembly / 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与非聚合物 | 实验方法 | 实验环境 | 结构质量 |
|---|---|---|---|---|---|---|---|
| 4EGX Crystal structure of KIF1A CC1-FHA tandem 提交 2012-04-02 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
430–607（178 aa）
片段：CC1-FHA tandem, UNP residues 430-607
链 B
430–607（178 aa）
片段：CC1-FHA tandem, UNP residues 430-607
|
未记录 | PEG DI(HYDROXYETHYL)ETHER × 1 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 5.8；289 K；18% (w/v) PEG 5000 MME, 8% (v/v) Tacsimate (pH 6.0), 0.1 M Bis-Tris, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
分辨率 2.51 Å R-free 0.283 |
| 4EGX Crystal structure of KIF1A CC1-FHA tandem 提交 2012-04-02 | Assembly 2 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 C
430–607（178 aa）
片段：CC1-FHA tandem, UNP residues 430-607
链 D
430–607（178 aa）
片段：CC1-FHA tandem, UNP residues 430-607
|
未记录 | PEG DI(HYDROXYETHYL)ETHER × 1 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 5.8；289 K；18% (w/v) PEG 5000 MME, 8% (v/v) Tacsimate (pH 6.0), 0.1 M Bis-Tris, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
分辨率 2.51 Å R-free 0.283 |
| 4EJQ Crystal structure of KIF1A C-CC1-FHA 提交 2012-04-06 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
458–607（150 aa）
片段：C-CC1-FHA, UNP residues 458-607
链 B
458–607（150 aa）
片段：C-CC1-FHA, UNP residues 458-607
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.2；289 K；0.2 M calcium acetate hydrate, 18% (w/v) PEG 3000, 0.1 M Bis-Tris, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
分辨率 1.89 Å R-free 0.231 |
| 4EJQ Crystal structure of KIF1A C-CC1-FHA 提交 2012-04-06 | Assembly 2 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 C
458–607（150 aa）
片段：C-CC1-FHA, UNP residues 458-607
链 D
458–607（150 aa）
片段：C-CC1-FHA, UNP residues 458-607
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.2；289 K；0.2 M calcium acetate hydrate, 18% (w/v) PEG 3000, 0.1 M Bis-Tris, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
分辨率 1.89 Å R-free 0.231 |
| 4EJQ Crystal structure of KIF1A C-CC1-FHA 提交 2012-04-06 | Assembly 3 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 E
458–607（150 aa）
片段：C-CC1-FHA, UNP residues 458-607
链 F
458–607（150 aa）
片段：C-CC1-FHA, UNP residues 458-607
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.2；289 K；0.2 M calcium acetate hydrate, 18% (w/v) PEG 3000, 0.1 M Bis-Tris, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
分辨率 1.89 Å R-free 0.231 |
| 4EJQ Crystal structure of KIF1A C-CC1-FHA 提交 2012-04-06 | Assembly 4 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 G
458–607（150 aa）
片段：C-CC1-FHA, UNP residues 458-607
链 H
458–607（150 aa）
片段：C-CC1-FHA, UNP residues 458-607
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.2；289 K；0.2 M calcium acetate hydrate, 18% (w/v) PEG 3000, 0.1 M Bis-Tris, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
分辨率 1.89 Å R-free 0.231 |
| 4UXO Conserved mechanisms of microtubule-stimulated ADP release, ATP binding, and force generation in transport kinesins 提交 2014-08-27 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 3 PDB 声明：trimeric（3） 与蛋白数一致 |
链 C
1–361（361 aa）
|
未记录 | ZN ZINC ION × 1 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
20MM PIPES, 2MM MGCL2, 1MM EGTA, 2MM DTT, 10 U/ML APYRASE；pH 6.8；20MM PIPES, 2MM MGCL2, 1MM EGTA, 2MM DTT, 10 U/ML APYRASE
cryo-EM玻璃化条件
冷冻剂 ETHANE；LIQUID ETHANE
|
分辨率 6.30 Å |
| 4UXP Conserved mechanisms of microtubule-stimulated ADP release, ATP binding, and force generation in transport kinesins 提交 2014-08-27 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 3 PDB 声明：trimeric（3） 与蛋白数一致 |
链 C
1–361（361 aa）
|
未记录 | ZN ZINC ION × 1 MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
20MM PIPES, 2MM MGCL2, 1MM EGTA, 2MM DTT, 10 U/ML APYRASE；pH 6.8；20MM PIPES, 2MM MGCL2, 1MM EGTA, 2MM DTT, 10 U/ML APYRASE
cryo-EM玻璃化条件
冷冻剂 ETHANE；LIQUID ETHANE
|
分辨率 6.30 Å |
| 4UXR Conserved mechanisms of microtubule-stimulated ADP release, ATP binding, and force generation in transport kinesins 提交 2014-08-27 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 3 PDB 声明：trimeric（3） 与蛋白数一致 |
链 C
1–361（361 aa）
|
未记录 | ZN ZINC ION × 1 MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ALF TETRAFLUOROALUMINATE ION × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 6.8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 7.00 Å |
| 4UXS Conserved mechanisms of microtubule-stimulated ADP release, ATP binding, and force generation in transport kinesins 提交 2014-08-27 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 3 PDB 声明：trimeric（3） 与蛋白数一致 |
链 C
1–361（361 aa）
|
未记录 | ZN ZINC ION × 1 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
20MM PIPES, 2MM MGCL2, 1MM EGTA, 2MM DTT, 2MM ADP；pH 6.8；20MM PIPES, 2MM MGCL2, 1MM EGTA, 2MM DTT, 2MM ADP
cryo-EM玻璃化条件
冷冻剂 ETHANE；LIQUID ETHANE
|
分辨率 7.00 Å |
| 8UTN KIF1A[1-393] AMP-PNP bound two-heads-bound state in complex with a microtubule (class T23L1) 提交 2023-10-31 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 7 PDB 声明：heptameric（7） 与蛋白数一致 |
链 K
1–393（393 aa）
片段：residues 1-393
链 N
1–393（393 aa）
片段：residues 1-393
|
未记录 | MG MAGNESIUM ION × 4 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 6.8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.10 Å |
| 8UTO KIF1A[1-393] AMP-PNP bound two-heads-bound state in complex with a microtubule - class T2L1 提交 2023-10-31 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 7 PDB 声明：heptameric（7） 与蛋白数一致 |
链 K
1–393（393 aa）
片段：residues 1-393
链 N
1–393（393 aa）
片段：residues 1-393
|
未记录 | MG MAGNESIUM ION × 4 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 6.8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.20 Å |
| 8UTP KIF1A[1-393] - AMP-PNP two-heads-bound state in complex with a microtubule - class T3L1 提交 2023-10-31 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 7 PDB 声明：heptameric（7） 与蛋白数一致 |
链 K
1–393（393 aa）
片段：residues 1-393
链 N
1–393（393 aa）
片段：residues 1-393
|
未记录 | MG MAGNESIUM ION × 4 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 6.8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.20 Å |
| 8UTQ KIF1A[1-393] AMP-PNP bound one-head-bound state in complex with a microtubule - class T1L02* 提交 2023-10-31 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 5 PDB 声明：pentameric（5） 与蛋白数一致 |
链 K
1–393（393 aa）
片段：residues 1-393
|
未记录 | MG MAGNESIUM ION × 3 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 6.8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.10 Å |
| 8UTR KIF1A[1-393] ADP bound in complex with a microtubule 提交 2023-10-31 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 3 PDB 声明：trimeric（3） 与蛋白数一致 |
链 K
1–393（393 aa）
片段：residues 1-393
|
未记录 | ADP ADENOSINE-5'-DIPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 6.8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.30 Å |
| 8UTS KIF1A[1-393] APO in complex with a microtubule 提交 2023-10-31 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 3 PDB 声明：trimeric（3） 与蛋白数一致 |
链 K
1–393（393 aa）
片段：residues 1-393
|
未记录 | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 6.8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.70 Å |
| 8UTT KIF1A[1-393] P305L mutant AMP-PNP bound two-heads-bound state in complex with a microtubule 提交 2023-10-31 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 7 PDB 声明：heptameric（7） 与蛋白数一致 |
链 K
1–393（393 aa）
片段：residues 1-393
链 N
1–393（393 aa）
片段：residues 1-393
|
突变：P305L 突变：P305L | MG MAGNESIUM ION × 4 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 6.8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.10 Å |
| 8UTU KIF1A[1-393] P305L mutant AMP-PNP bound one and two heads bound states merged, in complex with a microtubule 提交 2023-10-31 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 K
1–393（393 aa）
片段：residues 1-393
|
突变：P305L | MG MAGNESIUM ION × 3 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 6.8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.00 Å |
| 8UTV KIF1A[1-393] P305L mutant ADP bound in complex with a microtubule 提交 2023-10-31 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 K
1–393（393 aa）
片段：residues 1-393
|
突变：P305L | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 6.8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.00 Å |
| 8UTW KIF1A[1-393] P305L mutant APO in complex with a microtubule 提交 2023-10-31 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 3 PDB 声明：trimeric（3） 与蛋白数一致 |
链 K
1–393（393 aa）
片段：residues 1-393
|
突变：P305L | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 6.8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.40 Å |
| 8UTY KIF1A[1-393] P364L mutant AMP-PNP bound two-heads-bound state in complex with a microtubule 提交 2023-10-31 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 7 PDB 声明：heptameric（7） 与蛋白数一致 |
链 K
1–393（393 aa）
片段：residues 1-393
链 N
1–393（393 aa）
片段：residues 1-393
|
突变：P364L 突变：P364L | GTP GUANOSINE-5'-TRIPHOSPHATE × 3 MG MAGNESIUM ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 2 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 6.8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.30 Å |
| 9YA5 KIF1A R350G bound to microtubules in two-heads-bound state with AMP-PNP 提交 2025-09-15 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 6 PDB 声明：hexameric（6） 与蛋白数一致 |
链 K
1–393（393 aa）
链 N
1–393（393 aa）
|
突变：R350G 突变：R350G | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 2 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 6.8；BRB80 buffer composed of 80 mM
PIPES, 2 mM MgCl2, 1 mM EGTA, PH 6.8
cryo-EM玻璃化条件
冷冻剂 ETHANE；4uL of a 6uM MT solution in BRB80 (80mM PIPES, 2mM MgCl2, 1mM EGTA pH 6.8) along with 20uM paclitaxel was added onto a plasma-cleaned grid. The MTs were incubated for 1 minute at room temperature, and then the excess liquid was removed from the grid using Whatman #1 paper. Next, 4uL of a solution containing 20uM KIF1A-R350G in BRB80 supplemented with 20uM paclitaxel and 5mM AMP-PNP was added to the grid. The grid with the MT and kinesin mixture was then mounted into a Vitrobot, incubated for 1 minute at room temperature, and plunge-frozen into liquid nitrogen-cooled ethane.
|
分辨率 2.95 Å |
| 9YA7 KIF1A R350G bound to microtubules in the apo state 提交 2025-09-15 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 3 PDB 声明：trimeric（3） 与蛋白数一致 |
链 K
1–393（393 aa）
|
突变：R350 | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 6.8；BRB80 buffer composed of 80 mM
PIPES, 2 mM MgCl2, 1 mM EGTA, PH 6.8
cryo-EM玻璃化条件
冷冻剂 ETHANE；4uL of a 6uM MT solution in BRB80 (80mM PIPES, 2mM MgCl2, 1mM EGTA pH 6.8) along with 20uM paclitaxel was added onto a plasma-cleaned grid. The MTs were incubated for 1 minute at room temperature, and then the excess liquid was removed from the grid using Whatman #1 paper. Next, 4uL of a solution containing 20uM KIF1A-R350G in BRB80 supplemented with 20uM paclitaxel and 0.005 units per uL apyrase was added to the grid. The grid with the MT and kinesin mixture was then mounted into a Vitrobot, incubated for 1 minute at room temperature, and plunge-frozen into liquid nitrogen-cooled ethane.
|
分辨率 3.29 Å |
| 9YAB KIF1A R350W bound to microtubules in the apo state 提交 2025-09-15 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 3 PDB 声明：trimeric（3） 与蛋白数一致 |
链 K
1–393（393 aa）
|
突变：R350W | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 TA1 TAXOL × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 6.8；BRB80 buffer composed of 80 mM
PIPES, 2 mM MgCl2, 1 mM EGTA, PH 6.8
cryo-EM玻璃化条件
冷冻剂 ETHANE；4uL of a 6uM MT solution in BRB80 (80mM PIPES, 2mM MgCl2, 1mM EGTA pH 6.8) along with 20uM paclitaxel was added onto a plasma-cleaned grid. The MTs were incubated for 1 minute at room temperature, and then the excess liquid was removed from the grid using Whatman #1 paper. Next, 4uL of a solution containing 17.5uM KIF1A-R350W in BRB80 supplemented with 20uM paclitaxel and 0.005 units per uL apyrase was added to the grid. The grid with the MT and kinesin mixture was then mounted into a Vitrobot, incubated for 1 minute at room temperature, and plunge-frozen into liquid nitrogen-cooled ethane.
|
分辨率 3.21 Å |
| 9YAI KIF1A R350W bound to microtubules in two-heads-bound state with AMP-PNP 提交 2025-09-16 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 6 PDB 声明：hexameric（6） 与蛋白数一致 |
链 K
1–393（393 aa）
链 N
1–393（393 aa）
|
突变：R350W 突变：R350W | MG MAGNESIUM ION × 3 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 6.8；BRB80 buffer composed of 80 mM
PIPES, 2 mM MgCl2, 1 mM EGTA, PH 6.8
cryo-EM玻璃化条件
冷冻剂 ETHANE；4uL of a 6uM MT solution in BRB80 (80mM PIPES, 2mM MgCl2, 1mM EGTA pH 6.8) along with 20uM paclitaxel was added onto a plasma-cleaned grid. The MTs were incubated for 1 minute at room temperature, and then the excess liquid was removed from the grid using Whatman #1 paper. Next, 4uL of a solution containing 17.5uM KIF1A-R350W in BRB80 supplemented with 20uM paclitaxel and 5mM AMP-PNP was added to the grid. The grid with the MT and kinesin mixture was then mounted into a Vitrobot, incubated for 1 minute at room temperature, and plunge-frozen into liquid nitrogen-cooled ethane.
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分辨率 3.12 Å |