Current Protein Identity：Q5SLP7
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 2OM7 Structural Basis for Interaction of the Ribosome with the Switch Regions of GTP-bound Elongation Factors Deposited 2007-01-21 | Assembly 1 Protein–RNA Heteromer；Protein × 4 PDB declaration: tetradecameric（14） Consistent with all polymers |
Chain K
1–229（229 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
0.3 mM GMPPNP, 10 mM Hepes-KOH (pH 7.8), 10 mM Mg acetate, 60 mM NH4Cl, and 6 mM B-mercaptoethanol；pH 7.8；0.3 mM GMPPNP, 10 mM Hepes-KOH (pH 7.8), 10 mM Mg acetate, 60 mM NH4Cl, and 6 mM B-mercaptoethanol
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.30 Å |
| 4U1U Crystal structure of the E. coli ribosome bound to quinupristin. Deposited 2014-07-16 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain B5
2–229（228 aa）
|
Not recorded | MG MAGNESIUM ION × 271 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
EVAPORATION；pH 6.5；291 K；PEG8k, MPD
|
Resolution 2.95 Å R-free 0.282 |
| 4U1V Crystal structure of the E. coli ribosome bound to linopristin. Deposited 2014-07-16 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain B5
2–229（228 aa）
|
Not recorded | MG MAGNESIUM ION × 271 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
MICROBATCH；pH 6.5；291 K；PEG8k, MPD
|
Resolution 3.00 Å R-free 0.274 |
| 4U20 Crystal structure of the E. coli ribosome bound to flopristin. Deposited 2014-07-16 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain B5
2–229（228 aa）
|
Not recorded | MG MAGNESIUM ION × 271 VIF Flopristin × 1 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
MICROBATCH；pH 6.5；291 K；PEG8k, MPD
|
Resolution 2.90 Å R-free 0.279 |
| 4U24 Crystal structure of the E. coli ribosome bound to dalfopristin. Deposited 2014-07-16 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain B5
2–229（228 aa）
|
Not recorded | MG MAGNESIUM ION × 271 DOL 5-(2-DIETHYLAMINO-ETHANESULFONYL)-21-HYDROXY-10-ISOPROPYL-11,19-DIMETHYL-9,26-DIOXA-3,15,28-TRIAZA-TRICYCLO[23.2.1.00,255]OCTACOSA-1(27),12,17,19,25(28)-PENTAENE-2,8,14,23-TETRAONE × 1 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
MICROBATCH；pH 6.5；291 K；PEG8k, MPD
|
Resolution 2.90 Å R-free 0.264 |
| 4U25 Crystal structure of the E. coli ribosome bound to virginiamycin M1. Deposited 2014-07-16 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain B5
2–229（228 aa）
|
Not recorded | MG MAGNESIUM ION × 271 VIR VIRGINIAMYCIN M1 × 1 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
MICROBATCH；pH 6.5；291 K；PEG8k, MPD
|
Resolution 2.90 Å R-free 0.265 |
| 4U26 Crystal structure of the E. coli ribosome bound to dalfopristin and quinupristin. Deposited 2014-07-16 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain B5
2–229（228 aa）
|
Not recorded | MG MAGNESIUM ION × 271 DOL 5-(2-DIETHYLAMINO-ETHANESULFONYL)-21-HYDROXY-10-ISOPROPYL-11,19-DIMETHYL-9,26-DIOXA-3,15,28-TRIAZA-TRICYCLO[23.2.1.00,255]OCTACOSA-1(27),12,17,19,25(28)-PENTAENE-2,8,14,23-TETRAONE × 1 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
MICROBATCH；pH 6.5；291 K；PEG8k, MPD
|
Resolution 2.80 Å R-free 0.271 |
| 4U27 Crystal structure of the E. coli ribosome bound to flopristin and linopristin. Deposited 2014-07-16 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain B5
2–229（228 aa）
|
Not recorded | MG MAGNESIUM ION × 271 VIF Flopristin × 1 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
MICROBATCH；pH 6.5；291 K；PEG8k, MPD
|
Resolution 2.80 Å R-free 0.260 |
| 4V51 Structure of the Thermus thermophilus 70S ribosome complexed with mRNA, tRNA and paromomycin Deposited 2006-07-31 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain BC
1–228（228 aa）
|
Not recorded | MG MAGNESIUM ION × 741 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7；0.2M KSCN, 4% PEG 20K, 4% PEG550 MME, 0.1M TRIS-ACETATE PH 7
|
Resolution 2.80 Å R-free 0.313 |
| 4V51 Structure of the Thermus thermophilus 70S ribosome complexed with mRNA, tRNA and paromomycin Deposited 2006-07-31 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain DC
1–228（228 aa）
|
Not recorded | MG MAGNESIUM ION × 638 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7；0.2M KSCN, 4% PEG 20K, 4% PEG550 MME, 0.1M TRIS-ACETATE PH 7
|
Resolution 2.80 Å R-free 0.313 |
| 4V5A Structure of the Ribosome Recycling Factor bound to the Thermus thermophilus 70S ribosome with mRNA, ASL-Phe and tRNA-fMet Deposited 2007-06-28 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain BC
1–228（228 aa）
|
Not recorded | MG MAGNESIUM ION × 741 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7；COMPARE SELMER ET AL SCIENCE 2006, pH 7.0
|
Resolution 3.50 Å R-free 0.326 |
| 4V5A Structure of the Ribosome Recycling Factor bound to the Thermus thermophilus 70S ribosome with mRNA, ASL-Phe and tRNA-fMet Deposited 2007-06-28 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain DC
1–228（228 aa）
|
Not recorded | MG MAGNESIUM ION × 741 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7；COMPARE SELMER ET AL SCIENCE 2006, pH 7.0
|
Resolution 3.50 Å R-free 0.326 |
| 4V5C Structure of the Thermus thermophilus 70S ribosome in complex with mRNA, paromomycin, acylated A-site tRNA, deacylated P-site tRNA, and E-site tRNA. Deposited 2009-03-24 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain BC
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 741 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.1；pH 7.1
|
Resolution 3.30 Å R-free 0.272 |
| 4V5C Structure of the Thermus thermophilus 70S ribosome in complex with mRNA, paromomycin, acylated A-site tRNA, deacylated P-site tRNA, and E-site tRNA. Deposited 2009-03-24 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain DC
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 741 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.1；pH 7.1
|
Resolution 3.30 Å R-free 0.272 |
| 4V5D Structure of the Thermus thermophilus 70S ribosome in complex with mRNA, paromomycin, acylated A- and P-site tRNAs, and E-site tRNA. Deposited 2009-03-24 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain BC
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 673 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.1；pH 7.1
|
Resolution 3.50 Å R-free 0.256 |
| 4V5D Structure of the Thermus thermophilus 70S ribosome in complex with mRNA, paromomycin, acylated A- and P-site tRNAs, and E-site tRNA. Deposited 2009-03-24 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain DC
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 673 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.1；pH 7.1
|
Resolution 3.50 Å R-free 0.256 |
| 4V5E Insights into translational termination from the structure of RF2 bound to the ribosome Deposited 2009-04-30 | Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain BC
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 546 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.1；SEE SELMER ET AL., SCIENCE 2006, pH 7.1
|
Resolution 3.45 Å R-free 0.257 |
| 4V5E Insights into translational termination from the structure of RF2 bound to the ribosome Deposited 2009-04-30 | Assembly 2 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain DC
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 546 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.1；SEE SELMER ET AL., SCIENCE 2006, pH 7.1
|
Resolution 3.45 Å R-free 0.257 |
| 4V5F The structure of the ribosome with elongation factor G trapped in the post-translocational state Deposited 2009-09-01 | Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 62-meric（62） Consistent with all polymers |
Chain BC
1–229（229 aa）
|
Not recorded | ZN ZINC ION × 4 MG MAGNESIUM ION × 1 FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5；100 MM MES PH 6.5, 12%PEG 20K, 50 MM KCL, 10 MM NH4CL, 3UM DEOXY-BIG-CHAP
|
Resolution 3.60 Å R-free 0.260 |
| 4V5F The structure of the ribosome with elongation factor G trapped in the post-translocational state Deposited 2009-09-01 | Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 62-meric（62） Consistent with all polymers |
Chain DC
1–229（229 aa）
|
Not recorded | ZN ZINC ION × 4 MG MAGNESIUM ION × 1 FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5；100 MM MES PH 6.5, 12%PEG 20K, 50 MM KCL, 10 MM NH4CL, 3UM DEOXY-BIG-CHAP
|
Resolution 3.60 Å R-free 0.260 |
| 4V5G The crystal structure of the 70S ribosome bound to EF-Tu and tRNA Deposited 2009-09-01 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain BC
1–229（229 aa）
|
Not recorded | PAR PAROMOMYCIN × 1 ZN ZINC ION × 4 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 KIR KIRROMYCIN × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.3；100 MM MES PH 6.3, 22-25 MM KCL, 50 MM SUCROSE, 1% GLYCEROL, 5.4% (W/V) PEG20K
|
Resolution 3.60 Å R-free 0.315 |
| 4V5G The crystal structure of the 70S ribosome bound to EF-Tu and tRNA Deposited 2009-09-01 | Assembly 2 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain DC
1–229（229 aa）
|
Not recorded | PAR PAROMOMYCIN × 1 ZN ZINC ION × 4 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 KIR KIRROMYCIN × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.3；100 MM MES PH 6.3, 22-25 MM KCL, 50 MM SUCROSE, 1% GLYCEROL, 5.4% (W/V) PEG20K
|
Resolution 3.60 Å R-free 0.315 |
| 4V5J Structure of the 70S ribosome bound to Release factor 2 and a substrate analog provides insights into catalysis of peptide release Deposited 2010-03-24 | Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain BC
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 548 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5；SEE SELMER ET AL., SCIENCE 2006, pH 6.5
|
Resolution 3.10 Å R-free 0.264 |
| 4V5J Structure of the 70S ribosome bound to Release factor 2 and a substrate analog provides insights into catalysis of peptide release Deposited 2010-03-24 | Assembly 2 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain DC
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 546 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5；SEE SELMER ET AL., SCIENCE 2006, pH 6.5
|
Resolution 3.10 Å R-free 0.264 |
| 4V5K Structure of cytotoxic domain of colicin E3 bound to the 70S ribosome Deposited 2010-05-29 | Assembly 1 Insufficient information Heteromer；Protein × 52 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain BC
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 1164 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.18；AS STATED IN STRUCTURE OF THE 70S RIBOSOME COMPLEXED WITH MRNA AND TRNA. (2006) SCIENCE 313: 1935, PH 7.18
|
Resolution 3.20 Å R-free 0.270 |
| 4V5K Structure of cytotoxic domain of colicin E3 bound to the 70S ribosome Deposited 2010-05-29 | Assembly 2 Insufficient information Heteromer；Protein × 52 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain DC
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 1170 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.18；AS STATED IN STRUCTURE OF THE 70S RIBOSOME COMPLEXED WITH MRNA AND TRNA. (2006) SCIENCE 313: 1935, PH 7.18
|
Resolution 3.20 Å R-free 0.270 |
| 4V5L The structure of EF-Tu and aminoacyl-tRNA bound to the 70S ribosome with a GTP analog Deposited 2010-09-02 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain BC
1–229（229 aa）
|
Not recorded | PAR PAROMOMYCIN × 1 ZN ZINC ION × 4 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.3；100 MM MES PH 6.3, 60-100 MM KCL, 50 MM SUCROSE, 1% GLYCEROL, AND 5.3% (W/V) PEG20K
|
Resolution 3.10 Å R-free 0.268 |
| 4V5M tRNA tranlocation on the 70S ribosome: the pre-translocational translocation intermediate TI(PRE) Deposited 2010-10-01 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain BC
1–229（229 aa）
|
Not recorded | FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM vitrification conditions
Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, INSTRUMENT- VITROBOT (FEI)
|
Resolution 7.80 Å |
| 4V5N tRNA translocation on the 70S ribosome: the post- translocational translocation intermediate TI(POST) Deposited 2010-10-21 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain BC
1–229（229 aa）
|
Not recorded | FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM vitrification conditions
Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, INSTRUMENT- VITROBOT (FEI)
|
Resolution 7.60 Å |
| 4V5P The crystal structure of EF-Tu and A9C-tRNA-Trp bound to a near- cognate codon on the 70S ribosome Deposited 2010-12-07 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain BC
1–229（229 aa）
|
Not recorded | ZN ZINC ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 1 KIR KIRROMYCIN × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.3；100 MM MES PH 6.3, 60-100 MM KCL, 50 MM SUCROSE, 1% GLYCEROL, 5.2% (W/V) PEG20K
|
Resolution 3.10 Å R-free 0.267 |
| 4V5P The crystal structure of EF-Tu and A9C-tRNA-Trp bound to a near- cognate codon on the 70S ribosome Deposited 2010-12-07 | Assembly 2 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain DC
1–229（229 aa）
|
Not recorded | ZN ZINC ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 1 KIR KIRROMYCIN × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.3；100 MM MES PH 6.3, 60-100 MM KCL, 50 MM SUCROSE, 1% GLYCEROL, 5.2% (W/V) PEG20K
|
Resolution 3.10 Å R-free 0.267 |
| 4V5Q The crystal structure of EF-Tu and G24A-tRNA-Trp bound to a near- cognate codon on the 70S ribosome Deposited 2010-12-07 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain BC
1–229（229 aa）
|
Not recorded | ZN ZINC ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 1 KIR KIRROMYCIN × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.3；100 MM MES PH 6.3, 60-100 MM KCL, 50 MM SUCROSE, 1% GLYCEROL, 5.2% (W/V) PEG20K
|
Resolution 3.10 Å R-free 0.275 |
| 4V5Q The crystal structure of EF-Tu and G24A-tRNA-Trp bound to a near- cognate codon on the 70S ribosome Deposited 2010-12-07 | Assembly 2 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain DC
1–229（229 aa）
|
Not recorded | ZN ZINC ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 1 KIR KIRROMYCIN × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.3；100 MM MES PH 6.3, 60-100 MM KCL, 50 MM SUCROSE, 1% GLYCEROL, 5.2% (W/V) PEG20K
|
Resolution 3.10 Å R-free 0.275 |
| 4V5R The crystal structure of EF-Tu and Trp-tRNA-Trp bound to a cognate codon on the 70S ribosome. Deposited 2010-12-07 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain BC
1–229（229 aa）
|
Not recorded | ZN ZINC ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 1 KIR KIRROMYCIN × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.3；100 MM MES PH 6.3, 60-100 MM KCL, 50 MM SUCROSE, 1% GLYCEROL, 5.2% (W/V) PEG20K
|
Resolution 3.10 Å R-free 0.264 |
| 4V5R The crystal structure of EF-Tu and Trp-tRNA-Trp bound to a cognate codon on the 70S ribosome. Deposited 2010-12-07 | Assembly 2 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain DC
1–229（229 aa）
|
Not recorded | ZN ZINC ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 1 KIR KIRROMYCIN × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.3；100 MM MES PH 6.3, 60-100 MM KCL, 50 MM SUCROSE, 1% GLYCEROL, 5.2% (W/V) PEG20K
|
Resolution 3.10 Å R-free 0.264 |
| 4V5S The crystal structure of EF-Tu and G24A-tRNA-Trp bound to a cognate codon on the 70S ribosome. Deposited 2010-12-07 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain BC
1–229（229 aa）
|
Not recorded | ZN ZINC ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 1 KIR KIRROMYCIN × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.3；100 MM MES PH 6.3, 60-100 MM KCL, 50 MM SUCROSE, 1% GLYCEROL, 5.2% (W/V) PEG20K
|
Resolution 3.10 Å R-free 0.285 |
| 4V5S The crystal structure of EF-Tu and G24A-tRNA-Trp bound to a cognate codon on the 70S ribosome. Deposited 2010-12-07 | Assembly 2 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain DC
1–229（229 aa）
|
Not recorded | ZN ZINC ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 1 KIR KIRROMYCIN × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.3；100 MM MES PH 6.3, 60-100 MM KCL, 50 MM SUCROSE, 1% GLYCEROL, 5.2% (W/V) PEG20K
|
Resolution 3.10 Å R-free 0.285 |
| 4V68 T. thermophilus 70S ribosome in complex with mRNA, tRNAs and EF-Tu.GDP.kirromycin ternary complex, fitted to a 6.4 A Cryo-EM map. Deposited 2008-12-11 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 60-meric（60） Consistent with all polymers |
Chain BC
19–225（207 aa）
|
Not recorded | PHA PHENYLALANINAL × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MAU N-METHYL KIRROMYCIN × 1 BME BETA-MERCAPTOETHANOL × 1 | ELECTRON MICROSCOPY |
cryo-EM vitrification conditions
Cryogen METHANE；A Vitrobot was used to shock-freeze the sample in liquid methane.
|
Resolution 6.40 Å |
| 4V6A Structure of EF-P bound to the 70S ribosome. Deposited 2009-06-15 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain BC
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 611 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCL, 0.1 M Tris pH 7.6, 2.5 % Peg 20K, 7-12% MPD, 0.5 mM BME, vapor diffusion, temperature 292K
|
Resolution 3.10 Å R-free 0.302 |
| 4V6A Structure of EF-P bound to the 70S ribosome. Deposited 2009-06-15 | Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain DC
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 439 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCL, 0.1 M Tris pH 7.6, 2.5 % Peg 20K, 7-12% MPD, 0.5 mM BME, vapor diffusion, temperature 292K
|
Resolution 3.10 Å R-free 0.302 |
| 4V7J Structure of RelE nuclease bound to the 70S ribosome (precleavage state) Deposited 2009-11-02 | Assembly 1 Insufficient information Heteromer；Protein × 52 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain AC
1–229（229 aa）
|
Not recorded | ZN ZINC ION × 4 MG MAGNESIUM ION × 532 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.2；293 K；0.1M Tris-HAc, 0.2M KSCN, 3-4.5% w/v PEG 20k, 3-4.5% PEG 550 monomethylether, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 3.30 Å R-free 0.247 |
| 4V7J Structure of RelE nuclease bound to the 70S ribosome (precleavage state) Deposited 2009-11-02 | Assembly 2 Insufficient information Heteromer；Protein × 52 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain BC
1–229（229 aa）
|
Not recorded | ZN ZINC ION × 4 MG MAGNESIUM ION × 532 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.2；293 K；0.1M Tris-HAc, 0.2M KSCN, 3-4.5% w/v PEG 20k, 3-4.5% PEG 550 monomethylether, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 3.30 Å R-free 0.247 |
| 4V7K Structure of RelE nuclease bound to the 70S ribosome (postcleavage state) Deposited 2009-11-02 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain AC
1–229（229 aa）
|
Not recorded | ZN ZINC ION × 4 MG MAGNESIUM ION × 532 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.2；293 K；0.1 M Tris-HAc, 0.2 M KSCN, 3-4.5% PEG20K (W/V), 3-4.5% PEG550 MME, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 3.60 Å R-free 0.245 |
| 4V7K Structure of RelE nuclease bound to the 70S ribosome (postcleavage state) Deposited 2009-11-02 | Assembly 2 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain BC
1–229（229 aa）
|
Not recorded | ZN ZINC ION × 4 MG MAGNESIUM ION × 532 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.2；293 K；0.1 M Tris-HAc, 0.2 M KSCN, 3-4.5% PEG20K (W/V), 3-4.5% PEG550 MME, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 3.60 Å R-free 0.245 |
| 4V7L The structures of viomycin bound to the 70S ribosome. Deposited 2009-11-12 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain BC
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 577 ZN ZINC ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCL, 0.1 M Tris pH 7.6, 2.5% Peg 20K, 7-12% MPD, 0.5 mM BME, vapor diffusion, temperature 292K
|
Resolution 3.00 Å R-free 0.272 |
| 4V7L The structures of viomycin bound to the 70S ribosome. Deposited 2009-11-12 | Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain DC
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 388 ZN ZINC ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCL, 0.1 M Tris pH 7.6, 2.5% Peg 20K, 7-12% MPD, 0.5 mM BME, vapor diffusion, temperature 292K
|
Resolution 3.00 Å R-free 0.272 |
| 4V7M The structures of Capreomycin bound to the 70S ribosome. Deposited 2009-11-12 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain BC
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 483 ZN ZINC ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCL, 0.1 M Tris pH 7.6, 2.5% Peg 20K, 7-12% MPD, 0.5 mM BME, vapor diffusion, temperature 292K
|
Resolution 3.45 Å R-free 0.268 |
| 4V7M The structures of Capreomycin bound to the 70S ribosome. Deposited 2009-11-12 | Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain DC
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 359 ZN ZINC ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCL, 0.1 M Tris pH 7.6, 2.5% Peg 20K, 7-12% MPD, 0.5 mM BME, vapor diffusion, temperature 292K
|
Resolution 3.45 Å R-free 0.268 |
| 4V8J Crystal structure of the bacterial ribosome ram mutation G347U. Deposited 2011-12-20 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain BC
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 369 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；3.5-4.5% PEG 20K, 3.5-4.5% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10MM MGCL2, PH 7.0, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K
|
Resolution 3.90 Å R-free 0.269 |
| 4V8J Crystal structure of the bacterial ribosome ram mutation G347U. Deposited 2011-12-20 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain DC
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 371 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；3.5-4.5% PEG 20K, 3.5-4.5% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10MM MGCL2, PH 7.0, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K
|
Resolution 3.90 Å R-free 0.269 |
| 4V8N The crystal structure of agmatidine tRNA-Ile2 bound to the 70S ribosome in the A and P site. Deposited 2013-02-13 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain BC
1–229（229 aa）
|
Not recorded | ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.1；0.1 M TRIS-ACETATE PH 7, 0.2 M KSCN, 3.5-5.5% (W/V) PEG 20K, AND 3.5-5.5% (W/V) PEG 550 MONOMETHYL ETHER
|
Resolution 3.10 Å R-free 0.280 |
| 4V8N The crystal structure of agmatidine tRNA-Ile2 bound to the 70S ribosome in the A and P site. Deposited 2013-02-13 | Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain DC
1–229（229 aa）
|
Not recorded | ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.1；0.1 M TRIS-ACETATE PH 7, 0.2 M KSCN, 3.5-5.5% (W/V) PEG 20K, AND 3.5-5.5% (W/V) PEG 550 MONOMETHYL ETHER
|
Resolution 3.10 Å R-free 0.280 |
| 4V8O Crystal structure of the hybrid state of ribosome in complex with the guanosine triphosphatase release factor 3 Deposited 2011-07-26 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain BC
1–229（229 aa）
|
Not recorded | GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.7；100 MM MES-KOH PH 6.7, 20 MM KCL, 7.4% (W/V) PEG20K
|
Resolution 3.80 Å R-free 0.351 |
| 4V8Q Complex of SmpB, a tmRNA fragment and EF-Tu-GDP-Kirromycin with the 70S ribosome Deposited 2011-12-10 | Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 60-meric（60） Consistent with all polymers |
Chain AC
1–229（229 aa）
|
Not recorded | ZN ZINC ION × 3 MG MAGNESIUM ION × 2 KIR KIRROMYCIN × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5；100 MM MES PH 6.5, 20 MM KCL, 8% (W/V) PEG20K
|
Resolution 3.10 Å R-free 0.270 |
| 4V8U Crystal Structure of 70S Ribosome with Both Cognate tRNAs in the E and P Sites Representing an Authentic Elongation Complex. Deposited 2012-08-28 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain BC
1–229（229 aa）
|
Not recorded | ZN ZINC ION × 4 FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.4；0.1M MES, PH6.5, 8.5-9.0% PEG20K, 0-25MM KCL, pH 6.4
|
Resolution 3.70 Å R-free 0.249 |
| 4V8U Crystal Structure of 70S Ribosome with Both Cognate tRNAs in the E and P Sites Representing an Authentic Elongation Complex. Deposited 2012-08-28 | Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain DC
1–229（229 aa）
|
Not recorded | ZN ZINC ION × 4 FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.4；0.1M MES, PH6.5, 8.5-9.0% PEG20K, 0-25MM KCL, pH 6.4
|
Resolution 3.70 Å R-free 0.249 |
| 4V8X Structure of Thermus thermophilus ribosome Deposited 2013-07-19 | Assembly 1 Insufficient information Heteromer；Protein × 53 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain BC
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 352 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.1；0.1 M TRIS-HAC PH 7.2, 0.2 M KSCN, 4.1%-4.3% (W/V) PEG 20K AND 4.1%-4.3% (W/V) PEG 550MME
|
Resolution 3.35 Å R-free 0.261 |
| 4V8X Structure of Thermus thermophilus ribosome Deposited 2013-07-19 | Assembly 2 Insufficient information Heteromer；Protein × 53 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain DC
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 355 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.1；0.1 M TRIS-HAC PH 7.2, 0.2 M KSCN, 4.1%-4.3% (W/V) PEG 20K AND 4.1%-4.3% (W/V) PEG 550MME
|
Resolution 3.35 Å R-free 0.261 |
| 4V90 Thermus thermophilus Ribosome Deposited 2014-02-22 | Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain BC
2–229（228 aa）
|
Not recorded | MG MAGNESIUM ION × 523 ZN ZINC ION × 3 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5；0.1M MES PH 6.5, 5.8-6.8%PEG20K, 30-120 MM KCL AND 0.8-2.5% GLYCEROL
|
Resolution 2.95 Å R-free 0.244 |
| 4V97 Crystal structure of the bacterial ribosome ram mutation G299A. Deposited 2012-04-06 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain BC
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 457 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl2, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 3.52 Å R-free 0.249 |
| 4V97 Crystal structure of the bacterial ribosome ram mutation G299A. Deposited 2012-04-06 | Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain DC
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 576 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl2, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 3.52 Å R-free 0.249 |
| 4V9H Crystal structure of the ribosome bound to elongation factor G in the guanosine triphosphatase state Deposited 2013-03-25 | Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain BC
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 138 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.3；294 K；100mM MES pH 6.3, 75mM KCl, 6.0% PEG 20K, VAPOR DIFFUSION, HANGING DROP, temperature 294K
|
Resolution 2.86 Å R-free 0.250 |
| 4WF1 Crystal structure of the E. coli ribosome bound to negamycin. Deposited 2014-09-11 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain B5
19–225（207 aa）
|
Not recorded | MG MAGNESIUM ION × 271 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
MICROBATCH；pH 6.5；291 K；PEG8k, MPD
|
Resolution 3.09 Å R-free 0.244 |
| 4WOI 4,5-linked aminoglycoside antibiotics regulate the bacterial ribosome by targeting dynamic conformational processes within intersubunit bridge B2 Deposited 2014-10-15 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain B5
2–229（228 aa）
|
Not recorded | MG MAGNESIUM ION × 269 PAR PAROMOMYCIN × 6 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
MICROBATCH；pH 6.5；291 K；PEG8k, MPD, KCl, KSCN
|
Resolution 3.00 Å R-free 0.253 |
| 4WPO Crystal structure of the Thermus thermophilus 70S ribosome in complex with elongation factor G in the pre-translocational state Deposited 2014-10-20 | Assembly 1 Insufficient information Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain AC
2–229（228 aa）
|
Not recorded | MG MAGNESIUM ION × 1163 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.6-3.0% PEG-20K, 7-10% MPD, 0.5mM BME
|
Resolution 2.80 Å R-free 0.252 |
| 4WPO Crystal structure of the Thermus thermophilus 70S ribosome in complex with elongation factor G in the pre-translocational state Deposited 2014-10-20 | Assembly 2 Insufficient information Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain CC
2–229（228 aa）
|
Not recorded | MG MAGNESIUM ION × 893 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.6-3.0% PEG-20K, 7-10% MPD, 0.5mM BME
|
Resolution 2.80 Å R-free 0.252 |
| 4WQF Crystal structure of the Thermus thermophilus 70S ribosome in complex with elongation factor G and fusidic acid in the post-translocational state Deposited 2014-10-21 | Assembly 1 Insufficient information Heteromer；Protein × 52 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain AC
2–229（228 aa）
|
Not recorded | MG MAGNESIUM ION × 1147 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.6-3.0% PEG-20K, 7-10% MPD, 0.5mM BME
|
Resolution 2.80 Å R-free 0.255 |
| 4WQF Crystal structure of the Thermus thermophilus 70S ribosome in complex with elongation factor G and fusidic acid in the post-translocational state Deposited 2014-10-21 | Assembly 2 Insufficient information Heteromer；Protein × 52 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain CC
2–229（228 aa）
|
Not recorded | MG MAGNESIUM ION × 893 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.6-3.0% PEG-20K, 7-10% MPD, 0.5mM BME
|
Resolution 2.80 Å R-free 0.255 |
| 4WQU Crystal structure of the Thermus thermophilus 70S ribosome in complex with elongation factor G trapped by the antibiotic dityromycin Deposited 2014-10-22 | Assembly 1 Insufficient information Heteromer；Protein × 53 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain AC
2–229（228 aa）
|
Not recorded | MG MAGNESIUM ION × 1147 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.6-3.0% PEG-20K, 7-10% MPD, 0.5mM BME
|
Resolution 2.80 Å R-free 0.264 |
| 4WQU Crystal structure of the Thermus thermophilus 70S ribosome in complex with elongation factor G trapped by the antibiotic dityromycin Deposited 2014-10-22 | Assembly 2 Insufficient information Heteromer；Protein × 53 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain CC
2–229（228 aa）
|
Not recorded | MG MAGNESIUM ION × 893 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.6-3.0% PEG-20K, 7-10% MPD, 0.5mM BME
|
Resolution 2.80 Å R-free 0.264 |
| 4WQY Crystal structure of the Thermus thermophilus 70S ribosome in complex with elongation factor G in the post-translocational state (without fusitic acid) Deposited 2014-10-22 | Assembly 1 Insufficient information Heteromer；Protein × 52 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain AC
2–229（228 aa）
|
Not recorded | MG MAGNESIUM ION × 1155 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.6-3.0% PEG-20K, 7-10% MPD, 0.5mM BME
|
Resolution 2.80 Å R-free 0.266 |
| 4WQY Crystal structure of the Thermus thermophilus 70S ribosome in complex with elongation factor G in the post-translocational state (without fusitic acid) Deposited 2014-10-22 | Assembly 2 Insufficient information Heteromer；Protein × 52 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain CC
2–229（228 aa）
|
Not recorded | MG MAGNESIUM ION × 890 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.6-3.0% PEG-20K, 7-10% MPD, 0.5mM BME
|
Resolution 2.80 Å R-free 0.266 |
| 4WT8 Crystal Structure of bactobolin A bound to 70S ribosome-tRNA complex Deposited 2014-10-29 | Assembly 1 Insufficient information Heteromer；Protein × 51 PDB declaration: 61-meric（61） Review required |
Chain CA
19–111（93 aa）
|
Not recorded | PAR PAROMOMYCIN × 1 3V6 Bactobolin A × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
EVAPORATION；293 K；0.2M KSCN, 0.1M Tris-HAc pH7, 3.5-4.5%(w/v) PEG20K, 3.5-
4.5%(w/v) PEG550MME
|
Resolution 3.40 Å R-free 0.241 |
| 4WT8 Crystal Structure of bactobolin A bound to 70S ribosome-tRNA complex Deposited 2014-10-29 | Assembly 2 Insufficient information Heteromer；Protein × 51 PDB declaration: 62-meric（62） Review required |
Chain DA
19–111（93 aa）
|
Not recorded | PAR PAROMOMYCIN × 1 3V6 Bactobolin A × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
EVAPORATION；293 K；0.2M KSCN, 0.1M Tris-HAc pH7, 3.5-4.5%(w/v) PEG20K, 3.5-
4.5%(w/v) PEG550MME
|
Resolution 3.40 Å R-free 0.241 |
| 5EL4 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with a U-U mismatch in the first position Deposited 2015-11-04 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain 71
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 671 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
|
Resolution 3.15 Å R-free 0.251 |
| 5HAU Crystal structure of antimicrobial peptide Bac7(1-19) bound to the Thermus thermophilus 70S ribosome Deposited 2015-12-30 | Assembly 1 Insufficient information Heteromer；Protein × 53 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain 1C
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 1081 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；292 K；0.15M Arginine-HCl, 0.1M Tris-HCl pH 7.3-7.6, 2.6-2.9% PEG-20K, 9-10% MPD, 0.5 mM BME
|
Resolution 3.00 Å R-free 0.265 |
| 5HAU Crystal structure of antimicrobial peptide Bac7(1-19) bound to the Thermus thermophilus 70S ribosome Deposited 2015-12-30 | Assembly 2 Insufficient information Heteromer；Protein × 53 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain 2C
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 848 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；292 K；0.15M Arginine-HCl, 0.1M Tris-HCl pH 7.3-7.6, 2.6-2.9% PEG-20K, 9-10% MPD, 0.5 mM BME
|
Resolution 3.00 Å R-free 0.265 |
| 5OT7 Elongation factor G-ribosome complex captures in the absence of inhibitors. Deposited 2017-08-21 | Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain f
3–229（227 aa）
|
Not recorded | MG MAGNESIUM ION × 462 ZN ZINC ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 5UQ7 70S ribosome complex with dnaX mRNA stemloop and E-site tRNA ("in" conformation) Deposited 2017-02-07 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain C
3–229（227 aa）
|
Not recorded | ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 5UQ8 70S ribosome complex with dnaX mRNA stem-loop and E-site tRNA ("out" conformation) Deposited 2017-02-07 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain C
3–229（227 aa）
|
Not recorded | ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 6C5L Conformation of methylated GGQ in the Peptidyl Transferase Center during translation termination (T. thermophilus) Deposited 2018-01-16 | Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain BC
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 218 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；292 K；5 mM HEPES, pH 7.5, 10 mM magnesium acetate, 50 mM potassium chloride, 10 mM ammonium chloride, 6 mM BME
|
Resolution 3.20 Å R-free 0.247 |
| 6C5L Conformation of methylated GGQ in the Peptidyl Transferase Center during translation termination (T. thermophilus) Deposited 2018-01-16 | Assembly 2 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain DC
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 256 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；292 K；5 mM HEPES, pH 7.5, 10 mM magnesium acetate, 50 mM potassium chloride, 10 mM ammonium chloride, 6 mM BME
|
Resolution 3.20 Å R-free 0.247 |
| 6Q95 Structure of tmRNA SmpB bound in A site of T. thermophilus 70S ribosome Deposited 2018-12-17 | Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 61-meric（61） Consistent with all polymers |
Chain A
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 637 ZN ZINC ION × 3 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 7LH5 Crystal structure of the Thermus thermophilus 70S ribosome in complex with plazomicin, mRNA and tRNAs Deposited 2021-01-21 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain BC
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 743 EDS (2S)-4-amino-N-[(1R,2S,3S,4R,5S)-5-amino-4-{[(2S,3R)-3-amino-6-{[(2-hydroxyethyl)amino]methyl}-3,4-dihydro-2H-pyran-2-y l]oxy}-2-{[3-deoxy-4-C-methyl-3-(methylamino)-beta-L-arabinopyranosyl]oxy}-3-hydroxycyclohexyl]-2-hydroxybutanamide × 1 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；294.15 K；100 mM TRIS-HCl, pH 7.6,
3-3.2% (w/v) PEG 20K,
7-12% (v/v) MPD,
100-200 mM arginine,
0.5 mM Beta-mercaptoethanol
|
Resolution 3.27 Å R-free 0.277 |
| 7LH5 Crystal structure of the Thermus thermophilus 70S ribosome in complex with plazomicin, mRNA and tRNAs Deposited 2021-01-21 | Assembly 2 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain DC
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 633 EDS (2S)-4-amino-N-[(1R,2S,3S,4R,5S)-5-amino-4-{[(2S,3R)-3-amino-6-{[(2-hydroxyethyl)amino]methyl}-3,4-dihydro-2H-pyran-2-y l]oxy}-2-{[3-deoxy-4-C-methyl-3-(methylamino)-beta-L-arabinopyranosyl]oxy}-3-hydroxycyclohexyl]-2-hydroxybutanamide × 1 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；294.15 K；100 mM TRIS-HCl, pH 7.6,
3-3.2% (w/v) PEG 20K,
7-12% (v/v) MPD,
100-200 mM arginine,
0.5 mM Beta-mercaptoethanol
|
Resolution 3.27 Å R-free 0.277 |