Current Protein Identity：Q6DKI1
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 8FKP Human nucleolar pre-60S ribosomal subunit (State A1) Deposited 2022-12-21 | Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 47-meric（47） Consistent with all polymers |
Chain SI
1–255（255 aa）
|
Not recorded | MG MAGNESIUM ION × 54 ZN ZINC ION × 2 SF4 IRON/SULFUR CLUSTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；Four applications with manual blotting before last blotting with the Vitrobot.
|
Resolution 2.85 Å |
| 8FKQ Human nucleolar pre-60S ribosomal subunit (State A2) Deposited 2022-12-21 | Assembly 1 Protein–RNA Heteromer；Protein × 40 PDB declaration: 43-meric（43） Consistent with all polymers |
Chain SI
1–255（255 aa）
|
Not recorded | MG MAGNESIUM ION × 51 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；Four applications with manual blotting before last blotting with the vitrobot.
|
Resolution 2.76 Å |
| 8FKR Human nucleolar pre-60S ribosomal subunit (State B1) Deposited 2022-12-21 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 51-meric（51） Consistent with all polymers |
Chain SI
1–255（255 aa）
|
Not recorded | MG MAGNESIUM ION × 54 ZN ZINC ION × 2 SF4 IRON/SULFUR CLUSTER × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 K POTASSIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；Four applications with manual blotting before last blotting with the vitrobot.
|
Resolution 2.89 Å |
| 8FKS Human nucleolar pre-60S ribosomal subunit (State B2) Deposited 2022-12-21 | Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 47-meric（47） Consistent with all polymers |
Chain SI
1–255（255 aa）
|
Not recorded | MG MAGNESIUM ION × 61 ZN ZINC ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 K POTASSIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；Four applications with manual blotting before last blotting with the vitrobot.
|
Resolution 2.88 Å |
| 8FKT Human nucleolar pre-60S ribosomal subunit (State C1) Deposited 2022-12-21 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain SI
1–255（255 aa）
|
Not recorded | MG MAGNESIUM ION × 64 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 K POTASSIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；Four applications with manual blotting before last blotting with the vitrobot.
|
Resolution 2.81 Å |
| 8FKU Human nucleolar pre-60S ribosomal subunit (State C2) Deposited 2022-12-21 | Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 50-meric（50） Consistent with all polymers |
Chain SI
1–255（255 aa）
|
Not recorded | MG MAGNESIUM ION × 58 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 K POTASSIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；Four applications with manual blotting before last blotting with the vitrobot.
|
Resolution 2.82 Å |
| 8FKV Human nucleolar pre-60S ribosomal subunit (State D1) Deposited 2022-12-21 | Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain SI
1–255（255 aa）
|
Not recorded | MG MAGNESIUM ION × 65 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 K POTASSIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；Four applications with manual blotting before last blotting with the vitrobot.
|
Resolution 2.47 Å |
| 8FKW Human nucleolar pre-60S ribosomal subunit (State D2) Deposited 2022-12-21 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain SI
1–255（255 aa）
|
Not recorded | MG MAGNESIUM ION × 59 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 K POTASSIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；Four applications with manual blotting before last blotting with the vitrobot.
|
Resolution 2.50 Å |
| 8FKX Human nucleolar pre-60S ribosomal subunit (State E) Deposited 2022-12-21 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain SI
1–255（255 aa）
|
Not recorded | MG MAGNESIUM ION × 61 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 K POTASSIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；Four applications with manual blotting before last blotting with the vitrobot.
|
Resolution 2.59 Å |
| 8FKY Human nucleolar pre-60S ribosomal subunit (State F) Deposited 2022-12-21 | Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain SI
1–255（255 aa）
|
Not recorded | MG MAGNESIUM ION × 70 ZN ZINC ION × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 K POTASSIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；Four applications with manual blotting before last blotting with the vitrobot.
|
Resolution 2.67 Å |
| 8FKZ Human nucleolar pre-60S ribosomal subunit (State G) Deposited 2022-12-21 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 51-meric（51） Consistent with all polymers |
Chain SI
1–255（255 aa）
|
Not recorded | MG MAGNESIUM ION × 64 ZN ZINC ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 1 K POTASSIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；Four applications with manual blotting before last blotting with the vitrobot.
|
Resolution 3.04 Å |
| 8FL2 Human nuclear pre-60S ribosomal subunit (State I1) Deposited 2022-12-21 | Assembly 1 Protein–RNA Heteromer；Protein × 61 PDB declaration: 65-meric（65） Consistent with all polymers |
Chain SI
1–255（255 aa）
|
Not recorded | MG MAGNESIUM ION × 100 ZN ZINC ION × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 K POTASSIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；Four applications with manual blotting before last blotting with the vitrobot.
|
Resolution 2.67 Å |
| 8FL3 Human nuclear pre-60S ribosomal subunit (State I2) Deposited 2022-12-21 | Assembly 1 Protein–RNA Heteromer；Protein × 60 PDB declaration: 63-meric（63） Consistent with all polymers |
Chain SI
1–255（255 aa）
|
Not recorded | MG MAGNESIUM ION × 100 ZN ZINC ION × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 K POTASSIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；Four applications with manual blotting before last blotting with the vitrobot.
|
Resolution 2.53 Å |
| 8FL4 Human nuclear pre-60S ribosomal subunit (State I3) Deposited 2022-12-21 | Assembly 1 Protein–RNA Heteromer；Protein × 59 PDB declaration: 62-meric（62） Consistent with all polymers |
Chain SI
1–255（255 aa）
|
Not recorded | MG MAGNESIUM ION × 99 ZN ZINC ION × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 K POTASSIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；Four applications with manual blotting before last blotting with the vitrobot.
|
Resolution 2.89 Å |
| 8FL6 Human nuclear pre-60S ribosomal subunit (State J1) Deposited 2022-12-21 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain SI
1–255（255 aa）
|
Not recorded | MG MAGNESIUM ION × 88 ZN ZINC ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 K POTASSIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；Four applications with manual blotting before last blotting with the vitrobot.
|
Resolution 2.62 Å |
| 8FL7 Human nuclear pre-60S ribosomal subunit (State J2) Deposited 2022-12-21 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain SI
1–255（255 aa）
|
Not recorded | MG MAGNESIUM ION × 88 ZN ZINC ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 K POTASSIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；Four applications with manual blotting before last blotting with the vitrobot.
|
Resolution 2.55 Å |
| 8FLA Human nuclear pre-60S ribosomal subunit (State K1) Deposited 2022-12-21 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain SI
1–255（255 aa）
|
Not recorded | MG MAGNESIUM ION × 88 ZN ZINC ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 K POTASSIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；Four applications with manual blotting before last blotting with the vitrobot.
|
Resolution 2.63 Å |
| 8FLB Human nuclear pre-60S ribosomal subunit (State K2) Deposited 2022-12-21 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain SI
1–255（255 aa）
|
Not recorded | MG MAGNESIUM ION × 88 ZN ZINC ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 1 K POTASSIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；Four applications with manual blotting before last blotting with the vitrobot.
|
Resolution 2.55 Å |
| 8FLD Human nuclear pre-60S ribosomal subunit (State L1) Deposited 2022-12-21 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain SI
1–255（255 aa）
|
Not recorded | MG MAGNESIUM ION × 87 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；Four applications with manual blotting before last blotting with the Vitrobot.
|
Resolution 2.58 Å |
| 8FLE Human nuclear pre-60S ribosomal subunit (State L2) Deposited 2022-12-21 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric（52） Consistent with all polymers |
Chain SI
1–255（255 aa）
|
Not recorded | MG MAGNESIUM ION × 87 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；Four applications with manual blotting before last blotting with the Vitrobot.
|
Resolution 2.48 Å |
| 8INE human nuclear pre-60S ribosomal particle - State G' Deposited 2023-03-09 | Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain 3
1–255（255 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 8INF human nuclear pre-60S ribosomal particle - State F' Deposited 2023-03-09 | Assembly 1 Protein–RNA Heteromer；Protein × 57 PDB declaration: 61-meric（61） Consistent with all polymers |
Chain 3
1–255（255 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 8IPX human nuclear pre-60S ribosomal particle - State C' Deposited 2023-03-15 | Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain 1
1–255（255 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.30 Å |
| 8IPY human nuclear pre-60S ribosomal particle - State D' Deposited 2023-03-15 | Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain 1
1–255（255 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 8IR3 human nuclear pre-60S ribosomal particle - State B' Deposited 2023-03-17 | Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain 3
1–255（255 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |