Current Protein Identity:Q7LDG7
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 2MA2 Solution structure of RasGRP2 EF hands bound to calcium Deposited 2013-06-24 | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
417–497(81 aa)
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7;304 K;Ionic strength (raw mmCIF value) 0.125;Pressure ambient
NMR sample composition
500 uM [U-99% 13C; U-99% 15N] RasGRP2, 25 mM HEPES, 100 mM sodium chloride, 1 mM TCEP, 7 % D2O, 93% H2O/7% D2O | 93% H2O/7% D2O
NMR sample composition
500 uM [U-99% 13C; U-99% 15N] RasGRP2, 25 mM HEPES, 100 mM sodium chloride, 1 mM TCEP, 7 % D2O, 17 mg/mL Pf1 phage, 93% H2O/7% D2O | 93% H2O/7% D2O
|
Resolution not provided |
| 6AXF Structure of RasGRP2 in complex with Rap1B Deposited 2017-09-06 | Assembly 1 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain A
63–456(394 aa)
|
Non-standard monomer:Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 5.6;293 K;17% PEG3350 and 10 mM sodium citrate (pH 5.6)
|
Resolution 3.10 Å R-free 0.258 |
| 6AXF Structure of RasGRP2 in complex with Rap1B Deposited 2017-09-06 | Assembly 2 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain C
63–456(394 aa)
|
Non-standard monomer:Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 5.6;293 K;17% PEG3350 and 10 mM sodium citrate (pH 5.6)
|
Resolution 3.10 Å R-free 0.258 |
| 6AXF Structure of RasGRP2 in complex with Rap1B Deposited 2017-09-06 | Assembly 3 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain E
63–456(394 aa)
|
Non-standard monomer:Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 5.6;293 K;17% PEG3350 and 10 mM sodium citrate (pH 5.6)
|
Resolution 3.10 Å R-free 0.258 |
| 6AXF Structure of RasGRP2 in complex with Rap1B Deposited 2017-09-06 | Assembly 4 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain G
63–456(394 aa)
|
Non-standard monomer:Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 5.6;293 K;17% PEG3350 and 10 mM sodium citrate (pH 5.6)
|
Resolution 3.10 Å R-free 0.258 |
| 6AXF Structure of RasGRP2 in complex with Rap1B Deposited 2017-09-06 | Assembly 5 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain I
63–456(394 aa)
|
Non-standard monomer:Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 5.6;293 K;17% PEG3350 and 10 mM sodium citrate (pH 5.6)
|
Resolution 3.10 Å R-free 0.258 |
| 6AXF Structure of RasGRP2 in complex with Rap1B Deposited 2017-09-06 | Assembly 6 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain K
63–456(394 aa)
|
Non-standard monomer:Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 5.6;293 K;17% PEG3350 and 10 mM sodium citrate (pH 5.6)
|
Resolution 3.10 Å R-free 0.258 |
| 6AXF Structure of RasGRP2 in complex with Rap1B Deposited 2017-09-06 | Assembly 7 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain M
63–456(394 aa)
|
Non-standard monomer:Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 5.6;293 K;17% PEG3350 and 10 mM sodium citrate (pH 5.6)
|
Resolution 3.10 Å R-free 0.258 |
| 6AXF Structure of RasGRP2 in complex with Rap1B Deposited 2017-09-06 | Assembly 8 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain O
63–456(394 aa)
|
Non-standard monomer:Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 5.6;293 K;17% PEG3350 and 10 mM sodium citrate (pH 5.6)
|
Resolution 3.10 Å R-free 0.258 |