Current Protein Identity:Q7Z406
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 5I4E Crystal Structure of Human Nonmuscle Myosin 2C motor domain Deposited 2016-02-11 | Assembly 1 Insufficient information Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
47–784(738 aa)
Fragment:UNP residues 265-489,UNP residues 265-489
|
Not recorded | AOV ADP ORTHOVANADATE × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;281.15 K;TRIS PH 8.2, PEG 5K MME, MPD, NACL.
|
Resolution 2.25 Å R-free 0.241 |
| 5JLH Cryo-EM structure of a human cytoplasmic actomyosin complex at near-atomic resolution Deposited 2016-04-27 | Assembly 1 Insufficient information Heteromer;Protein × 11 PDB declaration: undecameric(11) Consistent with protein count |
Chain F
1–799(799 aa)
Chain G
1–799(799 aa)
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5;5 mM Tris-HCl pH 7.5, 1 mM DTT, 100 mM KCl, and 2 mM MgCl2
cryo-EM vitrification conditions
Cryogen ETHANE;Sample (2 uL of F-actin-tropomyosin solution) was applied to a glow-discharged holey carbon grid, incubated for 20 s and manually blotted from the backside for less than a second with filter paper. Afterwards 1.5 uL of myosin solution (3 uM without nucleotide) were added directly on the grid, incubated for 10 s and then manually blotted for 5 s from the backside with filter paper.
|
Resolution 3.90 Å |