当前蛋白身份：Q86V25
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相关结构差异明细
一行代表一个 PDB 条目中的一个 biological assembly；同一蛋白的多个单体会分别列出。
| PDB 条目 | Assembly / 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与非聚合物 | 实验方法 | 实验环境 | 结构质量 |
|---|---|---|---|---|---|---|---|
| 6J4O Structural basis of tubulin detyrosination by vasohibins-SVBP enzyme complex and functional implications 提交 2019-01-10 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
46–296（251 aa）
|
非标准单体：是（mmCIF未提供具体位点） | GOL GLYCEROL × 5 PO4 PHOSPHATE ION × 3 | X-RAY DIFFRACTION |
X-ray结晶条件
EVAPORATION；pH 8.2；293 K；1.6 M sodium/potassium phosphate, pH 8.2
|
分辨率 2.30 Å R-free 0.224 |
| 6J4P Structural basis of tubulin detyrosination by vasohibins-SVBP enzyme complex and functional implications 提交 2019-01-10 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
46–296（251 aa）
|
未记录 | BJL N-[(3R)-4-ethoxy-3-hydroxy-4-oxobutanoyl]-L-tyrosine × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
EVAPORATION；pH 7；293 K；V2c-SVBP was mixed with the inhibitor epoY at a mole ratio of 1:5 and incubated on ice for 30 min. Then, the resulting co-complex of V2c-SVBP-epoY was crystallized in 1.0 M lithium chloride, 0.1 M HEPES, pH 7.0, 20% PEG 6000.
|
分辨率 1.60 Å R-free 0.188 |
| 6J4Q Structural basis of tubulin detyrosination by vasohibins-SVBP enzyme complex and functional implications 提交 2019-01-10 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
46–296（251 aa）
|
未记录 | TQ8 N-[(2S)-4-chloro-3-oxo-1-phenyl-butan-2-yl]-4-methyl-benzenesulfonamide × 2 GOL GLYCEROL × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
EVAPORATION；pH 7；293 K；V2c-SVBP was mixed with the inhibitor TPCK at a mole ratio of 1:5 and incubated on ice for 30 min. Then, the resulting co-complex of V2c-SVBP-TPCK was crystallized in 1.0 M sodium malonate, pH 7.0.
|
分辨率 2.70 Å R-free 0.247 |
| 6J4Q Structural basis of tubulin detyrosination by vasohibins-SVBP enzyme complex and functional implications 提交 2019-01-10 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 C
46–296（251 aa）
|
未记录 | TQ8 N-[(2S)-4-chloro-3-oxo-1-phenyl-butan-2-yl]-4-methyl-benzenesulfonamide × 1 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
EVAPORATION；pH 7；293 K；V2c-SVBP was mixed with the inhibitor TPCK at a mole ratio of 1:5 and incubated on ice for 30 min. Then, the resulting co-complex of V2c-SVBP-TPCK was crystallized in 1.0 M sodium malonate, pH 7.0.
|
分辨率 2.70 Å R-free 0.247 |
| 6J4Q Structural basis of tubulin detyrosination by vasohibins-SVBP enzyme complex and functional implications 提交 2019-01-10 | Assembly 3 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 F
46–296（251 aa）
|
未记录 | TQ8 N-[(2S)-4-chloro-3-oxo-1-phenyl-butan-2-yl]-4-methyl-benzenesulfonamide × 2 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
EVAPORATION；pH 7；293 K；V2c-SVBP was mixed with the inhibitor TPCK at a mole ratio of 1:5 and incubated on ice for 30 min. Then, the resulting co-complex of V2c-SVBP-TPCK was crystallized in 1.0 M sodium malonate, pH 7.0.
|
分辨率 2.70 Å R-free 0.247 |
| 6J4Q Structural basis of tubulin detyrosination by vasohibins-SVBP enzyme complex and functional implications 提交 2019-01-10 | Assembly 4 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 J
46–296（251 aa）
|
未记录 | TQ8 N-[(2S)-4-chloro-3-oxo-1-phenyl-butan-2-yl]-4-methyl-benzenesulfonamide × 1 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
EVAPORATION；pH 7；293 K；V2c-SVBP was mixed with the inhibitor TPCK at a mole ratio of 1:5 and incubated on ice for 30 min. Then, the resulting co-complex of V2c-SVBP-TPCK was crystallized in 1.0 M sodium malonate, pH 7.0.
|
分辨率 2.70 Å R-free 0.247 |
| 6J4S Structural basis of tubulin detyrosination by vasohibins-SVBP enzyme complex and functional implications 提交 2019-01-10 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
1–355（355 aa）
|
未记录 | GOL GLYCEROL × 3 PO4 PHOSPHATE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
EVAPORATION；pH 6.8；293 K；full-length V2-SVBP complex at a concentration of 15 mg/mL was crystallized in 1.8 M sodium/potassium phosphate, pH 6.8
|
分辨率 2.80 Å R-free 0.227 |
| 6J4V Structural basis of tubulin detyrosination by vasohibins-SVBP enzyme complex and functional implications 提交 2019-01-10 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 3 PDB 声明：trimeric（3） 与蛋白数一致 |
链 A
46–296（251 aa）
|
未记录 | PO4 PHOSPHATE ION × 2 GOL GLYCEROL × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
EVAPORATION；pH 8.2；293 K；For crystallization of V2c-SVBP C58S in complex with the maTail peptide the components were mixed in a 1:3 molar ratio and incubated at room temperature for 24 h before setting up crystallization trials. The resulting V2c-SVBP S58C-maTail complex was crystallized in 1.4 M sodium/potassium phosphate, pH 8.2.
|
分辨率 2.10 Å R-free 0.200 |
| 6QBY Crystal structure of VASH 2 in complex with SVBP 提交 2018-12-24 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
40–295（256 aa）
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293.15 K；0.2M Sodium formate
0.1M Bis-Tris propane pH 6.5
20% PEG3350
|
分辨率 2.09 Å R-free 0.230 |
| 6QBY Crystal structure of VASH 2 in complex with SVBP 提交 2018-12-24 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 C
40–295（256 aa）
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293.15 K；0.2M Sodium formate
0.1M Bis-Tris propane pH 6.5
20% PEG3350
|
分辨率 2.09 Å R-free 0.230 |
| 7ZCW Cryo-EM structure of GMPCPP-microtubules in complex with VASH2-SVBP 提交 2022-03-29 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 8 PDB 声明：octameric（8） 与蛋白数一致 |
链 C
1–355（355 aa）
|
突变：C158A | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 6 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 4 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.60 Å |