Current Protein Identity：Q99848
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 8FKP Human nucleolar pre-60S ribosomal subunit (State A1) Deposited 2022-12-21 | Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 47-meric（47） Consistent with all polymers |
Chain SN
1–306（306 aa）
|
Not recorded | MG MAGNESIUM ION × 54 ZN ZINC ION × 2 SF4 IRON/SULFUR CLUSTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；Four applications with manual blotting before last blotting with the Vitrobot.
|
Resolution 2.85 Å |
| 8FKQ Human nucleolar pre-60S ribosomal subunit (State A2) Deposited 2022-12-21 | Assembly 1 Protein–RNA Heteromer；Protein × 40 PDB declaration: 43-meric（43） Consistent with all polymers |
Chain SN
1–306（306 aa）
|
Not recorded | MG MAGNESIUM ION × 51 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；Four applications with manual blotting before last blotting with the vitrobot.
|
Resolution 2.76 Å |
| 8FKR Human nucleolar pre-60S ribosomal subunit (State B1) Deposited 2022-12-21 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 51-meric（51） Consistent with all polymers |
Chain SN
1–306（306 aa）
|
Not recorded | MG MAGNESIUM ION × 54 ZN ZINC ION × 2 SF4 IRON/SULFUR CLUSTER × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 K POTASSIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；Four applications with manual blotting before last blotting with the vitrobot.
|
Resolution 2.89 Å |
| 8FKS Human nucleolar pre-60S ribosomal subunit (State B2) Deposited 2022-12-21 | Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 47-meric（47） Consistent with all polymers |
Chain SN
1–306（306 aa）
|
Not recorded | MG MAGNESIUM ION × 61 ZN ZINC ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 K POTASSIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；Four applications with manual blotting before last blotting with the vitrobot.
|
Resolution 2.88 Å |
| 8FKT Human nucleolar pre-60S ribosomal subunit (State C1) Deposited 2022-12-21 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain SN
1–306（306 aa）
|
Not recorded | MG MAGNESIUM ION × 64 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 K POTASSIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；Four applications with manual blotting before last blotting with the vitrobot.
|
Resolution 2.81 Å |
| 8FKU Human nucleolar pre-60S ribosomal subunit (State C2) Deposited 2022-12-21 | Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 50-meric（50） Consistent with all polymers |
Chain SN
1–306（306 aa）
|
Not recorded | MG MAGNESIUM ION × 58 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 K POTASSIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；Four applications with manual blotting before last blotting with the vitrobot.
|
Resolution 2.82 Å |
| 8FKV Human nucleolar pre-60S ribosomal subunit (State D1) Deposited 2022-12-21 | Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain SN
1–306（306 aa）
|
Not recorded | MG MAGNESIUM ION × 65 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 K POTASSIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；Four applications with manual blotting before last blotting with the vitrobot.
|
Resolution 2.47 Å |
| 8FKW Human nucleolar pre-60S ribosomal subunit (State D2) Deposited 2022-12-21 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain SN
1–306（306 aa）
|
Not recorded | MG MAGNESIUM ION × 59 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 K POTASSIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；Four applications with manual blotting before last blotting with the vitrobot.
|
Resolution 2.50 Å |
| 8FKX Human nucleolar pre-60S ribosomal subunit (State E) Deposited 2022-12-21 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain SN
1–306（306 aa）
|
Not recorded | MG MAGNESIUM ION × 61 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 K POTASSIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；Four applications with manual blotting before last blotting with the vitrobot.
|
Resolution 2.59 Å |
| 8FKY Human nucleolar pre-60S ribosomal subunit (State F) Deposited 2022-12-21 | Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain SN
1–306（306 aa）
|
Not recorded | MG MAGNESIUM ION × 70 ZN ZINC ION × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 K POTASSIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；Four applications with manual blotting before last blotting with the vitrobot.
|
Resolution 2.67 Å |