当前蛋白身份：Q99933
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差异标签只比较当前检索结果；所有PDB和assembly原始记录仍分别保留。
相关结构差异明细
一行代表一个 PDB 条目中的一个 biological assembly；同一蛋白的多个单体会分别列出。
| PDB 条目 | Assembly / 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与非聚合物 | 实验方法 | 实验环境 | 结构质量 |
|---|---|---|---|---|---|---|---|
| 1HX1 CRYSTAL STRUCTURE OF A BAG DOMAIN IN COMPLEX WITH THE HSC70 ATPASE DOMAIN 提交 2001-01-11 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 B
222–334（113 aa）
片段：BAG DOMAIN
|
未记录 | TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；PEG3350, Tris, Na-K-tartrate, glycerol, DTT, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 1.90 Å R-free 0.279 |
| 1WXV Solution structure of the ubiquitin domain of BCL-2 binding athanogene-1 提交 2005-02-02 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
73–151（79 aa）
片段：ubiquitin-like domain
|
未记录 | 未记录非水小分子 | SOLUTION NMR |
NMR测量条件
pH 7；298 K；离子强度（mmCIF原始值）100mM；压力 ambient
NMR样品组成
1.04mM ubiquitin domain U-13C, 15N; 20mM d-Tris-HCl; 100mM NaCl; 1mM d-DTT; 0.02 % NaN3 | 90% H2O/10% D2O
|
分辨率未提供 |
| 3FZF Crystal Structure of Hsc70/Bag1 in complex with ATP 提交 2009-01-26 | Assembly 2 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
222–334（113 aa）
片段：UNP residues 222-334
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；15% PEG3350, 0.1M Tris buffer, 25mM sodium-potassium tartrate, pH8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 2.20 Å R-free 0.292 |
| 3FZH Crystal Structures of Hsc70/Bag1 in Complex with Small Molecule Inhibitors 提交 2009-01-26 | Assembly 2 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
222–334（113 aa）
片段：UNP residues 222-334
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；15% PEG3350, 0.1M Tris buffer, 25mM sodium-potassium tartrate, pH8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 2.00 Å R-free 0.272 |
| 3FZK Crystal Structures of Hsc70/Bag1 in Complex with Small Molecule Inhibitors 提交 2009-01-26 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 B
222–334（113 aa）
片段：UNP residues 222-334
|
未记录 | TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 CL CHLORIDE ION × 1 3BK (2R,3R,4S,5R)-2-[6-amino-8-[(3,4-dichlorophenyl)methylamino]purin-9-yl]-5-(hydroxymethyl)oxolane-3,4-diol × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；15% PEG3350, 0.1M Tris buffer, 25mM sodium-potassium tartrate, pH8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 2.10 Å R-free 0.294 |
| 3FZL Crystal Structures of Hsc70/Bag1 in Complex with Small Molecule Inhibitors 提交 2009-01-26 | Assembly 2 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
222–334（113 aa）
片段：UNP residues 222-334
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；15% PEG3350, 0.1M Tris buffer, 25mM sodium-potassium tartrate, pH8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 2.20 Å R-free 0.304 |
| 3FZM Crystal Structures of Hsc70/Bag1 in Complex with Small Molecule Inhibitors 提交 2009-01-26 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 B
222–334（113 aa）
片段：UNP residues 222-334
|
未记录 | 3GO 4-[[(2R,3S,4R,5R)-5-[6-amino-8-(quinolin-6-ylmethylamino)purin-9-yl]-3,4-dihydroxy-oxolan-2-yl]methoxymethyl]benzonitri le × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；15% PEG3350, 0.1M Tris buffer, 25mM sodium-potassium tartrate, pH8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 2.30 Å R-free 0.254 |
| 3LDQ Crystal structure of HSC70/BAG1 in complex with small molecule inhibitor 提交 2010-01-13 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 B
222–334（113 aa）
|
未记录 | 3P1 8-[(quinolin-2-ylmethyl)amino]adenosine × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；15% PEG3350, 0.1M TRIS BUFFER, 25MM SODIUM-POTASSIUM TARTRATE, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 1.90 Å R-free 0.256 |
| 3M3Z Crystal structure of HSC70/BAG1 in complex with small molecule inhibitor 提交 2010-03-10 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 B
222–334（113 aa）
|
未记录 | 3F5 5'-O-(2-amino-2-oxoethyl)-8-(methylamino)adenosine × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；15% PEG3350, 0.1M TRIS BUFFER, 25MM SODIUM-POTASSIUM TARTRATE, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 2.10 Å R-free 0.293 |
| 5AQF Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 B
222–334（113 aa）
片段：RESIDUES 222-334
|
未记录 | ADN ADENOSINE × 1 GOL GLYCEROL × 8 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；HSC70/BAG1 COMPLEX AT 10 MG/ML INCUBATED WITH 5 MM ADENOSINE, MIXED 1:1 WITH 16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 1.88 Å R-free 0.208 |
| 5AQF Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 D
222–334（113 aa）
片段：RESIDUES 222-334
|
未记录 | ADN ADENOSINE × 1 GOL GLYCEROL × 4 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；HSC70/BAG1 COMPLEX AT 10 MG/ML INCUBATED WITH 5 MM ADENOSINE, MIXED 1:1 WITH 16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 1.88 Å R-free 0.208 |
| 5AQG Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 B
222–334（113 aa）
片段：RESIDUES 222-334
|
未记录 | ZJB (2R,3R,4S,5R)-2-(3-AMINO-5-METHYL-1,4,5,6,8-PENTAAZAACENAPHTHYLEN-1(5H)-YL)-5-(HYDROXYMETHYL)TETRAHYDROFURAN-3,4-DIOL × 1 GOL GLYCEROL × 2 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 2.24 Å R-free 0.222 |
| 5AQG Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 D
222–334（113 aa）
片段：RESIDUES 222-334
|
未记录 | ZJB (2R,3R,4S,5R)-2-(3-AMINO-5-METHYL-1,4,5,6,8-PENTAAZAACENAPHTHYLEN-1(5H)-YL)-5-(HYDROXYMETHYL)TETRAHYDROFURAN-3,4-DIOL × 1 GOL GLYCEROL × 6 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 2.24 Å R-free 0.222 |
| 5AQG Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 3 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 F
222–334（113 aa）
片段：RESIDUES 222-334
|
未记录 | ZJB (2R,3R,4S,5R)-2-(3-AMINO-5-METHYL-1,4,5,6,8-PENTAAZAACENAPHTHYLEN-1(5H)-YL)-5-(HYDROXYMETHYL)TETRAHYDROFURAN-3,4-DIOL × 1 GOL GLYCEROL × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 2.24 Å R-free 0.222 |
| 5AQH Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 B
222–334（113 aa）
片段：RESIDUES 222-334
|
未记录 | ZVO 5-methyl-1,5-dihydro-1,4,5,6,8-pentaazaacenaphthylen-3-amine × 1 GOL GLYCEROL × 7 DMS DIMETHYL SULFOXIDE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 2.00 Å R-free 0.228 |
| 5AQI Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 B
222–334（113 aa）
片段：RESIDUES 222-334
|
未记录 | DMS DIMETHYL SULFOXIDE × 2 GOL GLYCEROL × 7 ADE ADENINE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 1.98 Å R-free 0.242 |
| 5AQI Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 D
222–334（113 aa）
片段：RESIDUES 222-334
|
未记录 | DMS DIMETHYL SULFOXIDE × 1 GOL GLYCEROL × 4 ADE ADENINE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 1.98 Å R-free 0.242 |
| 5AQJ Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 F
222–334（113 aa）
片段：RESIDUES 222-334
|
未记录 | CL CHLORIDE ION × 1 Q88 9H-purine-6,8-diamine × 1 GOL GLYCEROL × 5 DMS DIMETHYL SULFOXIDE × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 1.96 Å R-free 0.221 |
| 5AQJ Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 B
222–334（113 aa）
片段：RESIDUES 222-334
|
未记录 | CL CHLORIDE ION × 1 Q88 9H-purine-6,8-diamine × 1 GOL GLYCEROL × 3 DMS DIMETHYL SULFOXIDE × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 1.96 Å R-free 0.221 |
| 5AQJ Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 3 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 D
222–334（113 aa）
片段：RESIDUES 222-334
|
未记录 | CL CHLORIDE ION × 1 Q88 9H-purine-6,8-diamine × 1 GOL GLYCEROL × 8 DMS DIMETHYL SULFOXIDE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 1.96 Å R-free 0.221 |
| 5AQK Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 B
222–334（113 aa）
片段：RESIDUES 222-334
|
未记录 | GDP GUANOSINE-5'-DIPHOSPHATE × 2 GOL GLYCEROL × 3 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 DMS DIMETHYL SULFOXIDE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5, 25% (V/V) GLYCEROL AND 100 MM GDP FROM 1 M STOCK AT PH 7.5
|
分辨率 2.09 Å R-free 0.232 |
| 5AQL Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 B
222–334（113 aa）
片段：RESIDUES 222-334
|
未记录 | TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 2 GOL GLYCEROL × 3 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 1.69 Å R-free 0.197 |
| 5AQL Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 D
222–334（113 aa）
片段：RESIDUES 222-334
|
未记录 | TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 2 GOL GLYCEROL × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 1.69 Å R-free 0.197 |
| 5AQM Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 B
222–334（113 aa）
片段：RESIDUES 222-334
|
未记录 | TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 2 GOL GLYCEROL × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 1.63 Å R-free 0.195 |
| 5AQM Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 D
222–334（113 aa）
片段：RESIDUES 222-334
|
未记录 | TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 2 GOL GLYCEROL × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 1.63 Å R-free 0.195 |
| 5AQN Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 B
222–334（113 aa）
片段：RESIDUES 222 TO 334
|
未记录 | JG8 BENZOFURO[3,2-D]PYRIMIDIN-4(3H)-ONE × 1 GOL GLYCEROL × 4 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 DMS DIMETHYL SULFOXIDE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 2.45 Å R-free 0.228 |
| 5AQN Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 D
222–334（113 aa）
片段：RESIDUES 222 TO 334
|
未记录 | JG8 BENZOFURO[3,2-D]PYRIMIDIN-4(3H)-ONE × 1 GOL GLYCEROL × 3 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 DMS DIMETHYL SULFOXIDE × 1 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 2.45 Å R-free 0.228 |
| 5AQN Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 3 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 F
222–334（113 aa）
片段：RESIDUES 222 TO 334
|
未记录 | JG8 BENZOFURO[3,2-D]PYRIMIDIN-4(3H)-ONE × 1 GOL GLYCEROL × 2 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 DMS DIMETHYL SULFOXIDE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 2.45 Å R-free 0.228 |
| 5AQO Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 B
222–334（113 aa）
片段：RESIDUES 222 TO 334
|
未记录 | GOL GLYCEROL × 6 CWS 6-METHYLQUINAZOLIN-4-AMINE × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 DMS DIMETHYL SULFOXIDE × 1 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 2.12 Å R-free 0.210 |
| 5AQO Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 D
222–334（113 aa）
片段：RESIDUES 222 TO 334
|
未记录 | GOL GLYCEROL × 10 CWS 6-METHYLQUINAZOLIN-4-AMINE × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 DMS DIMETHYL SULFOXIDE × 1 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 2.12 Å R-free 0.210 |
| 5AQO Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 3 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 F
222–334（113 aa）
片段：RESIDUES 222 TO 334
|
未记录 | GOL GLYCEROL × 5 CWS 6-METHYLQUINAZOLIN-4-AMINE × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 DMS DIMETHYL SULFOXIDE × 1 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 2.12 Å R-free 0.210 |
| 5AQP Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 D
222–334（113 aa）
片段：UNP RESIDUES 222-334
|
未记录 | 1LQ quinazolin-4-amine × 1 GOL GLYCEROL × 8 DMS DIMETHYL SULFOXIDE × 2 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 2.08 Å R-free 0.209 |
| 5AQP Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 F
222–334（113 aa）
片段：UNP RESIDUES 222-334
|
未记录 | 1LQ quinazolin-4-amine × 1 GOL GLYCEROL × 4 DMS DIMETHYL SULFOXIDE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 2.08 Å R-free 0.209 |
| 5AQP Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 3 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 B
222–334（113 aa）
片段：UNP RESIDUES 222-334
|
未记录 | 1LQ quinazolin-4-amine × 1 GOL GLYCEROL × 2 DMS DIMETHYL SULFOXIDE × 2 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 2.08 Å R-free 0.209 |
| 5AQQ Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 F
222–334（113 aa）
片段：UNP RESIDUES 222-334
|
未记录 | BBW 7-methylquinazolin-4-amine × 1 DMS DIMETHYL SULFOXIDE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 2.72 Å R-free 0.241 |
| 5AQQ Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 B
222–334（113 aa）
片段：UNP RESIDUES 222-334
|
未记录 | BBW 7-methylquinazolin-4-amine × 1 DMS DIMETHYL SULFOXIDE × 1 GOL GLYCEROL × 2 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 2.72 Å R-free 0.241 |
| 5AQQ Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 3 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 D
222–334（113 aa）
片段：UNP RESIDUES 222-334
|
未记录 | BBW 7-methylquinazolin-4-amine × 1 DMS DIMETHYL SULFOXIDE × 1 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 2.72 Å R-free 0.241 |
| 5AQR Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 B
222–334（113 aa）
片段：UNP RESIDUES 222-334
|
未记录 | TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 N8Y 6-methoxyquinazolin-4-amine × 1 GOL GLYCEROL × 11 DMS DIMETHYL SULFOXIDE × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 1.91 Å R-free 0.236 |
| 5AQR Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 D
222–334（113 aa）
片段：UNP RESIDUES 222-334
|
未记录 | TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 N8Y 6-methoxyquinazolin-4-amine × 1 GOL GLYCEROL × 5 DMS DIMETHYL SULFOXIDE × 1 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 1.91 Å R-free 0.236 |
| 5AQR Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 3 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 F
222–334（113 aa）
片段：UNP RESIDUES 222-334
|
未记录 | TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 N8Y 6-methoxyquinazolin-4-amine × 1 GOL GLYCEROL × 2 DMS DIMETHYL SULFOXIDE × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 1.91 Å R-free 0.236 |
| 5AQS Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 D
222–334（113 aa）
片段：RESIDUES 222 TO 334
|
未记录 | 1SQ ISOQUINOLIN-1-AMINE × 1 GOL GLYCEROL × 3 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 2.00 Å R-free 0.261 |
| 5AQS Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 B
222–334（113 aa）
片段：RESIDUES 222 TO 334
|
未记录 | 1SQ ISOQUINOLIN-1-AMINE × 1 GOL GLYCEROL × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 2.00 Å R-free 0.261 |
| 5AQT Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 B
222–334（113 aa）
片段：UNP RESIDUES 222-334
|
未记录 | 5P7 (1S,2R,3R,5R)-3-(hydroxymethyl)-5-(quinazolin-4-ylamino)cyclopentane-1,2-diol × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 DMS DIMETHYL SULFOXIDE × 2 GOL GLYCEROL × 6 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 1.90 Å R-free 0.222 |
| 5AQU Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 B
222–334（113 aa）
片段：UNP RESIDUES 222-334
|
未记录 | GOL GLYCEROL × 9 UX0 (1S,2R,3R,5R)-3-(HYDROXYMETHYL)-5-((5-METHOXYQUINAZOLIN-4-YL)AMINO)CYCLOPENTANE-1,2-DIOL × 1 DMS DIMETHYL SULFOXIDE × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 1.92 Å R-free 0.201 |
| 5AQV Fragment-based screening of HSP70 sheds light on the functional role of ATP-binding site residues 提交 2015-09-22 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 B
222–334（113 aa）
片段：UNP RESIDUES 222-334
|
未记录 | KC7 (1R,2S,3R,5R)-3-((5-(benzyloxy)quinazolin-4-yl)amino)-5-(hydroxymethyl)cyclopentane-1,2-diol × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 GOL GLYCEROL × 7 DMS DIMETHYL SULFOXIDE × 3 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；16-26% (W/V) PEG3350, 0.1 M K-NA TARTRATE, 0.1 M TRIS.HCL PH 8.5 AND 25% (V/V) GLYCEROL
|
分辨率 1.75 Å R-free 0.197 |
| 9HEU Co-chaperone Bag1-bound human 26S proteasome in SBAG1 state 提交 2024-11-14 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 47 PDB 声明：47-meric（47） 与蛋白数一致 |
链 x
116–345（230 aa）
|
未记录 | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 5 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.6
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.60 Å |
| 9SZT Co-chaperone Bag1-bound human 26S proteasome in SBAG1.2 state 提交 2025-10-15 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 47 PDB 声明：47-meric（47） 与蛋白数一致 |
链 x
116–345（230 aa）
|
未记录 | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 5 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.6
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.50 Å |
| 9SZV Co-chaperone Bag1-bound human 26S proteasome in SBAG1.3 state 提交 2025-10-15 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 47 PDB 声明：47-meric（47） 与蛋白数一致 |
链 x
116–345（230 aa）
|
未记录 | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 5 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.6
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.60 Å |