当前蛋白身份：Q9JHU4
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差异标签只比较当前检索结果；所有PDB和assembly原始记录仍分别保留。
相关结构差异明细
一行代表一个 PDB 条目中的一个 biological assembly；同一蛋白的多个单体会分别列出。
| PDB 条目 | Assembly / 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与非聚合物 | 实验方法 | 实验环境 | 结构质量 |
|---|---|---|---|---|---|---|---|
| 3ERR Microtubule binding domain from mouse cytoplasmic dynein as a fusion with seryl-tRNA synthetase 提交 2008-10-03 | Assembly 1 信息不足 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
3260–3427（168 aa）
链 B
3260–3427（168 aa）
|
突变：C3323A, C3387A 突变：C3323A, C3387A | AMP ADENOSINE MONOPHOSPHATE × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；300 K；Protein was changed into crystallization buffer (20mM K-HEPES, pH7.5, 10% w/v glycerol, 0.2mM PMSF, 1mM DTT, 4mM Mg-ATP, 0.01% Na-Azide) and concentrated to 18 mg/ml. Crystallization was carried out by setting hanging drops containing 2 ul of protein, (diluted to 13.5mg/ml with 20mM K-Hepes, pH 7.5, 10% glycerol), 0.3 ul 70% glycerol and 1.8 ul of precipitant (20% PEG 4000, 200mM Ammonium sulfate, 100mM Bis-Tris, pH 5.5) over 500ml of the same precipitant solution. Crystals appeared within one day and were of dimensions up to 200 um., VAPOR DIFFUSION, HANGING DROP, temperature 300K
|
分辨率 2.27 Å R-free 0.247 |
| 3J1T High affinity dynein microtubule binding domain - tubulin complex 提交 2012-06-25 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 3 PDB 声明：trimeric（3） 与蛋白数一致 |
链 A
3264–3427（164 aa）
片段：SEE REMARK 999
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
50 mM Tris HCl, 1 mM MgCl2, 1mM EGTA, 1mM DTT；pH 8；50 mM Tris HCl, 1 mM MgCl2, 1mM EGTA, 1mM DTT
cryo-EM玻璃化条件
冷冻剂 ETHANE；The solution was blotted manually, and the process of addition and blotting of SRS-MTBD was repeated a total of three times. The final blotting was done inside the humidity chamber of a Vitrobot Mark IV (FEI) at 22 Celsius.
|
分辨率 9.70 Å |
| 3J1U Low affinity dynein microtubule binding domain - tubulin complex 提交 2012-06-25 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 3 PDB 声明：trimeric（3） 与蛋白数一致 |
链 A
3264–3427（164 aa）
片段：SEE REMARK 999
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
50 mM Tris HCl, 1 mM MgCl2, 1mM EGTA, 1mM DTT；pH 8；50 mM Tris HCl, 1 mM MgCl2, 1mM EGTA, 1mM DTT
cryo-EM玻璃化条件
冷冻剂 ETHANE；The solution was blotted manually, and the process of addition and blotting of SRS-MTBD was repeated a total of three times. The final blotting was done inside the humidity chamber of a Vitrobot Mark IV (FEI) at 22 Celsius.
|
分辨率 9.70 Å |
| 3WUQ Structure of the entire stalk region of the dynein motor domain 提交 2014-05-01 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
3207–3483（277 aa）
片段：STALK DOMAIN, UNP residues 3207-3483
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；277 K；20% PEG3350, 0.2M ammonium phosphate, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
分辨率 3.50 Å R-free 0.305 |
| 5AYH Structure of the entire dynein stalk region 提交 2015-08-21 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
3207–3475（269 aa）
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；277 K；PEG 3350, ammonium phosphate monobasic
|
分辨率 3.01 Å R-free 0.295 |
| 6RZA Cryo-EM structure of the human inner arm dynein DNAH7 microtubule binding domain bound to microtubules 提交 2019-06-13 | Assembly 1 信息不足 异源复合物；蛋白 × 5 PDB 声明：pentameric（5） 与蛋白数一致 |
链 X
3270–3284（15 aa）
链 X
3410–3418（9 aa）
|
未记录 | GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 2 TA1 TAXOL × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 4.50 Å |