Current Protein Identity：Q9NVX2
New Search
Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 8FL0 Human nucleolar pre-60S ribosomal subunit (State H) Deposited 2022-12-21 | Assembly 1 Protein–RNA Heteromer；Protein × 29 PDB declaration: 31-meric（31） Consistent with all polymers |
Chain NJ
1–485（485 aa）
|
Not recorded | MG MAGNESIUM ION × 22 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 K POTASSIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；Four applications with manual blotting before last blotting with the vitrobot.
|
Resolution 2.91 Å |
| 8FL2 Human nuclear pre-60S ribosomal subunit (State I1) Deposited 2022-12-21 | Assembly 1 Protein–RNA Heteromer；Protein × 61 PDB declaration: 65-meric（65） Consistent with all polymers |
Chain NJ
1–485（485 aa）
|
Not recorded | MG MAGNESIUM ION × 100 ZN ZINC ION × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 K POTASSIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；Four applications with manual blotting before last blotting with the vitrobot.
|
Resolution 2.67 Å |
| 8FL3 Human nuclear pre-60S ribosomal subunit (State I2) Deposited 2022-12-21 | Assembly 1 Protein–RNA Heteromer；Protein × 60 PDB declaration: 63-meric（63） Consistent with all polymers |
Chain NJ
1–485（485 aa）
|
Not recorded | MG MAGNESIUM ION × 100 ZN ZINC ION × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 K POTASSIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；Four applications with manual blotting before last blotting with the vitrobot.
|
Resolution 2.53 Å |
| 8FL4 Human nuclear pre-60S ribosomal subunit (State I3) Deposited 2022-12-21 | Assembly 1 Protein–RNA Heteromer；Protein × 59 PDB declaration: 62-meric（62） Consistent with all polymers |
Chain NJ
1–485（485 aa）
|
Not recorded | MG MAGNESIUM ION × 99 ZN ZINC ION × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 K POTASSIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE；Four applications with manual blotting before last blotting with the vitrobot.
|
Resolution 2.89 Å |
| 8INK human nuclear pre-60S ribosomal particle - State D Deposited 2023-03-10 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain W
1–485（485 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 8IPD human nuclear pre-60S ribosomal particle - State C Deposited 2023-03-14 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric（52） Consistent with all polymers |
Chain W
1–485（485 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 8IPX human nuclear pre-60S ribosomal particle - State C' Deposited 2023-03-15 | Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain W
1–485（485 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.30 Å |
| 8IPY human nuclear pre-60S ribosomal particle - State D' Deposited 2023-03-15 | Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain W
1–485（485 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 8IR1 human nuclear pre-60S ribosomal particle - State A Deposited 2023-03-17 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric（53） Consistent with all polymers |
Chain N
1–485（485 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 8IR3 human nuclear pre-60S ribosomal particle - State B' Deposited 2023-03-17 | Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain N
1–485（485 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 8QL1 Crystal structure of the human MDN1-MIDAS/NLE1-UBL complex Deposited 2023-09-19 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
10–104（95 aa）
|
Not recorded | MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；0.2 M Ammonium sulfate, 18.2% v/v Polyethylene glycol 300, 0.1 M Sodium Acetate, 3.5 M Ammonium chloride
|
Resolution 2.30 Å R-free 0.248 |
| 8QL1 Crystal structure of the human MDN1-MIDAS/NLE1-UBL complex Deposited 2023-09-19 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain D
10–104（95 aa）
|
Not recorded | MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；0.2 M Ammonium sulfate, 18.2% v/v Polyethylene glycol 300, 0.1 M Sodium Acetate, 3.5 M Ammonium chloride
|
Resolution 2.30 Å R-free 0.248 |
| 8QL1 Crystal structure of the human MDN1-MIDAS/NLE1-UBL complex Deposited 2023-09-19 | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain F
10–104（95 aa）
|
Not recorded | MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；0.2 M Ammonium sulfate, 18.2% v/v Polyethylene glycol 300, 0.1 M Sodium Acetate, 3.5 M Ammonium chloride
|
Resolution 2.30 Å R-free 0.248 |
| 8QL1 Crystal structure of the human MDN1-MIDAS/NLE1-UBL complex Deposited 2023-09-19 | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain H
10–104（95 aa）
|
Not recorded | MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；0.2 M Ammonium sulfate, 18.2% v/v Polyethylene glycol 300, 0.1 M Sodium Acetate, 3.5 M Ammonium chloride
|
Resolution 2.30 Å R-free 0.248 |
| 8RL2 Human pre-60S - State 5 Deposited 2024-01-02 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric（52） Consistent with all polymers |
Chain CG
1–485（485 aa）
|
Mutation：E85A | ZN ZINC ION × 5 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.84 Å |
| 9QIW Human pre-60S - State 3 Deposited 2025-03-17 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 51-meric（51） Consistent with all polymers |
Chain CG
1–485（485 aa）
|
Mutation：E85A | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.04 Å |