Current Protein Identity:Q9UBP0
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 3EAB Crystal structure of Spastin MIT in complex with ESCRT III Deposited 2008-08-25 | Assembly 1 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain A
112–196(85 aa)
Fragment:UNP residues 112 to 196
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 4.6;288 K;10% PEG 2000 MME, 0.2 M Ammonia Sulfate, 0.1 M Sodium Acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 288K
|
Resolution 2.50 Å R-free 0.268 |
| 3EAB Crystal structure of Spastin MIT in complex with ESCRT III Deposited 2008-08-25 | Assembly 2 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain B
112–196(85 aa)
Fragment:UNP residues 112 to 196
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 4.6;288 K;10% PEG 2000 MME, 0.2 M Ammonia Sulfate, 0.1 M Sodium Acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 288K
|
Resolution 2.50 Å R-free 0.268 |
| 3EAB Crystal structure of Spastin MIT in complex with ESCRT III Deposited 2008-08-25 | Assembly 3 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain C
112–196(85 aa)
Fragment:UNP residues 112 to 196
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 4.6;288 K;10% PEG 2000 MME, 0.2 M Ammonia Sulfate, 0.1 M Sodium Acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 288K
|
Resolution 2.50 Å R-free 0.268 |
| 3EAB Crystal structure of Spastin MIT in complex with ESCRT III Deposited 2008-08-25 | Assembly 4 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain D
112–196(85 aa)
Fragment:UNP residues 112 to 196
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 4.6;288 K;10% PEG 2000 MME, 0.2 M Ammonia Sulfate, 0.1 M Sodium Acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 288K
|
Resolution 2.50 Å R-free 0.268 |
| 3EAB Crystal structure of Spastin MIT in complex with ESCRT III Deposited 2008-08-25 | Assembly 5 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain E
112–196(85 aa)
Fragment:UNP residues 112 to 196
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 4.6;288 K;10% PEG 2000 MME, 0.2 M Ammonia Sulfate, 0.1 M Sodium Acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 288K
|
Resolution 2.50 Å R-free 0.268 |
| 3EAB Crystal structure of Spastin MIT in complex with ESCRT III Deposited 2008-08-25 | Assembly 6 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain F
112–196(85 aa)
Fragment:UNP residues 112 to 196
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 4.6;288 K;10% PEG 2000 MME, 0.2 M Ammonia Sulfate, 0.1 M Sodium Acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 288K
|
Resolution 2.50 Å R-free 0.268 |
| 3VFD Human spastin AAA domain Deposited 2012-01-09 | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
228–616(389 aa)
Fragment:AAA domain
|
Mutation:E442Q | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6.5;296 K;1.6M (NH3)2SO4, 0.1M MES monohydrate (pH 6.5),10% 1,4-dioxane and 4% benzamidine HCl, 1mM ATP, VAPOR DIFFUSION, HANGING DROP, temperature 296K
|
Resolution 3.30 Å R-free 0.275 |
| 5Z6Q Crystal structure of AAA of Spastin Deposited 2018-01-25 | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
229–616(388 aa)
Fragment:UNP residues 229-616
|
Not recorded | CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;293 K;3M (NH4)2SO4, 100mM NH4Cl
|
Resolution 3.00 Å R-free 0.276 |
| 5Z6R SPASTIN AAA WITH ATP Deposited 2018-01-25 | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
229–616(388 aa)
Fragment:UNP RESIDUES 229-616
|
Mutation:E442Q | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 8.2;293 K;25MM TRIS-HCL, 1MM DTT, 1MM MGCL2, 1MM ATP, 2% PEG 3350, 20MM KSCN
|
Resolution 3.00 Å R-free 0.279 |
| 6PEK Structure of Spastin Hexamer (Subunit A-E) in complex with substrate peptide Deposited 2019-06-20 | Assembly 1 Protein heterocomplex Heteromer;Protein × 6 PDB declaration: hexameric(6) Consistent with protein count |
Chain A
87–584(498 aa)
Chain B
87–584(498 aa)
Chain C
87–584(498 aa)
Chain D
87–584(498 aa)
Chain E
87–584(498 aa)
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 5 BEF BERYLLIUM TRIFLUORIDE ION × 4 MG MAGNESIUM ION × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.20 Å |
| 6PEN Structure of Spastin Hexamer (whole model) in complex with substrate peptide Deposited 2019-06-20 | Assembly 1 Protein heterocomplex Heteromer;Protein × 7 PDB declaration: heptameric(7) Consistent with protein count |
Chain A
87–584(498 aa)
Chain B
87–584(498 aa)
Chain C
87–584(498 aa)
Chain D
87–584(498 aa)
Chain E
87–584(498 aa)
Chain F
87–584(498 aa)
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 5 BEF BERYLLIUM TRIFLUORIDE ION × 4 MG MAGNESIUM ION × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.20 Å |
| 7S7J Structure of Human SPASTIN-IST1 complex. Deposited 2021-09-16 | Assembly 1 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain A
112–195(84 aa)
|
Not recorded | PG4 TETRAETHYLENE GLYCOL × 1 CA CALCIUM ION × 1 PGE TRIETHYLENE GLYCOL × 1 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 6.5;278 K;40% v/v PEG 300, 100 mM Sodium cacodylate / Hydrochloric acid pH=6.5, 200 mM Calcium Acetate
|
Resolution 1.15 Å R-free 0.157 |