当前蛋白身份:Q9UPY8
重新检索
差异标签只比较当前检索结果;所有PDB和assembly原始记录仍分别保留。
相关结构差异明细
一行代表一个 PDB 条目中的一个 biological assembly;同一蛋白的多个单体会分别列出。
| PDB 条目 | Assembly / 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与非聚合物 | 实验方法 | 实验环境 | 结构质量 |
|---|---|---|---|---|---|---|---|
| 1WYO Solution structure of the CH domain of human microtubule-associated protein RP/EB family member 3 提交 2005-02-15 | Assembly 1 蛋白单体 单体;蛋白 × 1 PDB 声明:monomeric(1) 与蛋白数一致 |
链 A
1–146(146 aa)
片段:CH domain
|
未记录 | 未记录非水小分子 | SOLUTION NMR |
NMR测量条件
pH 7;296 K;离子强度(mmCIF原始值)120mM;压力 ambient
NMR样品组成
1.28mM CH domain U-15N,13C; 20mM d-Tris-HCl(pH 7.0); 100mM NaCl; 1mM d-DTT; 0.02% NaN3; 10% D2O | 90% H2O/10% D2O
|
分辨率未提供 |
| 3CO1 Crystal structure of microtubule binding domain of human EB3 提交 2008-03-27 | Assembly 1 蛋白单体 单体;蛋白 × 1 PDB 声明:monomeric(1) 与蛋白数一致 |
链 A
1–130(130 aa)
片段:CH domain
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP;pH 7.4;298 K;0.04M potassium phosphate, 16% PEG 8000, 20% glycerol anhydrous, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
分辨率 1.40 Å R-free 0.234 |
| 3JAK Cryo-EM structure of GTPgammaS-microtubule co-polymerized with EB3 (merged dataset with and without kinesin bound) 提交 2015-06-16 | Assembly 1 蛋白异源复合物 异源复合物;蛋白 × 14 PDB 声明:tetradecameric(14) 与蛋白数一致 |
链 M
1–200(200 aa)
片段:UNP residues 1-200
链 N
1–200(200 aa)
片段:UNP residues 1-200
|
未记录 | GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 GSP 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE × 6 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
BRB80;pH 6.8;80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40
cryo-EM玻璃化条件
Blot once for 4 seconds before plunging.;90.4 K;冷冻剂 ETHANE;Blot once for 4 seconds before plunging into liquid ethane (FEI VITROBOT MARK II).
|
分辨率 3.30 Å |
| 3JAL Cryo-EM structure of GMPCPP-microtubule co-polymerized with EB3 提交 2015-06-16 | Assembly 1 蛋白异源复合物 异源复合物;蛋白 × 14 PDB 声明:tetradecameric(14) 与蛋白数一致 |
链 M
1–200(200 aa)
片段:UNP residues 1-200
链 N
1–200(200 aa)
片段:UNP residues 1-200
|
未记录 | GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 6 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
BRB80;pH 6.8;80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40
cryo-EM玻璃化条件
Blot once for 4 seconds before plunging;90.4 K;冷冻剂 ETHANE;Blot once for 4 seconds before plunging into liquid ethane (FEI VITROBOT MARK II).
|
分辨率 3.50 Å |
| 3JAR Cryo-EM structure of GDP-microtubule co-polymerized with EB3 提交 2015-06-19 | Assembly 1 蛋白异源复合物 异源复合物;蛋白 × 14 PDB 声明:tetradecameric(14) 与蛋白数一致 |
链 M
1–200(200 aa)
片段:UNP residues 1-200
链 N
1–200(200 aa)
片段:UNP residues 1-200
|
未记录 | GTP GUANOSINE-5'-TRIPHOSPHATE × 6 MG MAGNESIUM ION × 6 GDP GUANOSINE-5'-DIPHOSPHATE × 6 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
BRB80;pH 6.8;80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM DTT, 0.05% Nonidet P-40
cryo-EM玻璃化条件
Blot once for 4 seconds before plunging/;90.4 K;冷冻剂 ETHANE;Blot once for 4 seconds before plunging into liquid ethane (FEI VITROBOT MARK II).
|
分辨率 3.40 Å |
| 3TQ7 EB1c/EB3c heterodimer in complex with the CAP-Gly domain of P150glued 提交 2011-09-09 | Assembly 1 蛋白异源复合物 异源复合物;蛋白 × 4 PDB 声明:tetrameric(4) 与蛋白数一致 |
链 B
200–281(82 aa)
片段:EB3 c-terminal domain
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP;pH 8.5;293 K;15% PEG 4000, 0.1M Tris-HCl, 0.2M Magnesium chloride hexahydrate, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
分辨率 2.30 Å R-free 0.281 |
| 7SJ9 13pf E254A microtubule from recombinant human tubulin decorated with EB3 提交 2021-10-16 | Assembly 1 蛋白异源复合物 异源复合物;蛋白 × 14 PDB 声明:tetradecameric(14) 与蛋白数一致 |
链 M
1–281(281 aa)
链 N
1–281(281 aa)
|
未记录 | GTP GUANOSINE-5'-TRIPHOSPHATE × 12 MG MAGNESIUM ION × 12 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 6.8;80 mM PIPES, 1 mM EGTA, 5 mM MgCl2, 2 mM GTP, pH 6.8
cryo-EM玻璃化条件
冷冻剂 ETHANE-PROPANE
|
分辨率 3.80 Å |
| 9F3R 13pf E254Q microtubule from recombinant human tubulin decorated with EB3 提交 2024-04-25 | Assembly 1 蛋白异源复合物 异源复合物;蛋白 × 14 PDB 声明:14-meric(14) 与蛋白数一致 |
链 S
1–131(131 aa)
链 T
1–131(131 aa)
|
未记录 | GTP GUANOSINE-5'-TRIPHOSPHATE × 12 MG MAGNESIUM ION × 12 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 6.8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 4.30 Å |
| 9F3S 13pf mosaic 20%E254Q - 80% E254N microtubule from recombinant human tubulin decorated with EB3 提交 2024-04-25 | Assembly 1 蛋白异源复合物 异源复合物;蛋白 × 14 PDB 声明:14-meric(14) 与蛋白数一致 |
链 S
1–131(131 aa)
链 T
1–131(131 aa)
|
未记录 | GTP GUANOSINE-5'-TRIPHOSPHATE × 12 MG MAGNESIUM ION × 12 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 6.8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 4.20 Å |