Current Protein Identity:Q9Y580
New Search
Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 2M8H RRM domain of human RBM7 Deposited 2013-05-21 | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
4–94(91 aa)
Fragment:unp residues 4-94
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7;293.15 K;Ionic strength (raw mmCIF value) 200;Pressure ambient
NMR sample composition
0.3 mM [U-99% 13C; U-99% 15N] RBM7, 20 mM BISTRIS, 4 mM beta-mercaptoethanol, 200 mM sodium chloride, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 5IQQ Crystal structure of the human RBM7 RRM domain Deposited 2016-03-11 | Assembly 1 Protein homooligomer Homooligomer;Protein × 5 PDB declaration: pentameric(5) Consistent with protein count |
Chain A
1–91(91 aa)
Fragment:RNA recognition motif, UNP residues 1-91
Chain B
1–91(91 aa)
Fragment:RNA recognition motif, UNP residues 1-91
Chain C
1–91(91 aa)
Fragment:RNA recognition motif, UNP residues 1-91
Chain D
1–91(91 aa)
Fragment:RNA recognition motif, UNP residues 1-91
Chain E
1–91(91 aa)
Fragment:RNA recognition motif, UNP residues 1-91
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
EVAPORATION;pH 7.5;292 K;0.1 M Hepes pH 7.5, 150 mM KCl, 1.4 M tri-sodium citrate
|
Resolution 2.52 Å R-free 0.244 |
| 5LXR Structure of the minimal RBM7 - ZCCHC8 Complex Deposited 2016-09-22 | Assembly 1 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain A
1–86(86 aa)
|
Not recorded | SM SAMARIUM (III) ION × 3 BR BROMIDE ION × 4 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6.5;291.15 K;0.1 M Bis-Tris-Propane pH 6.5
0.2 M NaBr
18% (v/v) PEG3350
7% (w/v) glycerol
|
Resolution 2.00 Å R-free 0.229 |
| 5LXY Structure of the minimal RBM7 - ZCCHC8 Complex Deposited 2016-09-23 | Assembly 1 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain A
1–86(86 aa)
|
Not recorded | BR BROMIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6.5;291.15 K;0.1 M Bis-Tris-Propane
0.2 M NaBr
0.1 M Sodium malonate
20% (w/v) PEG3350
|
Resolution 2.85 Å R-free 0.300 |
| 5LXY Structure of the minimal RBM7 - ZCCHC8 Complex Deposited 2016-09-23 | Assembly 2 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain B
1–86(86 aa)
|
Not recorded | BR BROMIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6.5;291.15 K;0.1 M Bis-Tris-Propane
0.2 M NaBr
0.1 M Sodium malonate
20% (w/v) PEG3350
|
Resolution 2.85 Å R-free 0.300 |
| 5LXY Structure of the minimal RBM7 - ZCCHC8 Complex Deposited 2016-09-23 | Assembly 3 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain E
1–86(86 aa)
|
Not recorded | BR BROMIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6.5;291.15 K;0.1 M Bis-Tris-Propane
0.2 M NaBr
0.1 M Sodium malonate
20% (w/v) PEG3350
|
Resolution 2.85 Å R-free 0.300 |
| 5LXY Structure of the minimal RBM7 - ZCCHC8 Complex Deposited 2016-09-23 | Assembly 4 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain G
1–86(86 aa)
|
Not recorded | BR BROMIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6.5;291.15 K;0.1 M Bis-Tris-Propane
0.2 M NaBr
0.1 M Sodium malonate
20% (w/v) PEG3350
|
Resolution 2.85 Å R-free 0.300 |
| 5LXY Structure of the minimal RBM7 - ZCCHC8 Complex Deposited 2016-09-23 | Assembly 5 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain I
1–86(86 aa)
|
Not recorded | BR BROMIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6.5;291.15 K;0.1 M Bis-Tris-Propane
0.2 M NaBr
0.1 M Sodium malonate
20% (w/v) PEG3350
|
Resolution 2.85 Å R-free 0.300 |
| 5LXY Structure of the minimal RBM7 - ZCCHC8 Complex Deposited 2016-09-23 | Assembly 6 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain K
1–86(86 aa)
|
Not recorded | BR BROMIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6.5;291.15 K;0.1 M Bis-Tris-Propane
0.2 M NaBr
0.1 M Sodium malonate
20% (w/v) PEG3350
|
Resolution 2.85 Å R-free 0.300 |
| 5LXY Structure of the minimal RBM7 - ZCCHC8 Complex Deposited 2016-09-23 | Assembly 7 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain M
1–86(86 aa)
|
Not recorded | BR BROMIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6.5;291.15 K;0.1 M Bis-Tris-Propane
0.2 M NaBr
0.1 M Sodium malonate
20% (w/v) PEG3350
|
Resolution 2.85 Å R-free 0.300 |
| 7S7B Human Nuclear exosome targeting (NEXT) complex homodimer bound to RNA (substrate 1) Deposited 2021-09-15 | Assembly 1 Protein–RNA Heteromer;Protein × 6 PDB declaration: octameric(8) Consistent with all polymers |
Chain C
7–86(80 aa)
Chain G
7–86(80 aa)
|
Not recorded | ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8;20 mM Tris-Cl pH 8.0, 50 mM NaCl, 0.1 mM TCEP supplemented with 0.02% (v/v) IGEPAL CA-630
cryo-EM vitrification conditions
Cryogen ETHANE;30 s wait time, blot for 2.5 s before plunging
|
Resolution 4.06 Å |
| 7S7C Human Nuclear Exosome Targeting (NEXT) complex bound to RNA (substrate 2) Deposited 2021-09-15 | Assembly 1 Protein–RNA Heteromer;Protein × 5 PDB declaration: heptameric(7) Consistent with all polymers |
Chain C
7–86(80 aa)
|
Not recorded | ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE;30 s wait time, blot for 2.5 s before plunging
|
Resolution 3.62 Å |