SASDA56

BAZ2B in Tris

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2023-05-25T11:21:52.654103+02:00

1. 样品、组分与实验条件 Sample & Experiment

样品 1 · BAZ2B in Tris

浓度— – 10.0 mg/ml缓冲液 / pH20 mM Tris 500 mM NaCl 2 mM DTT 10 microM ZnCl2 / 8.0
Experimental temperature10.0 设备 / 束线DORIS III, DESY / EMBL X33
波长0.15 nm曝光15.0 s × 8

分子组分

组分类型 / OrganismUniProt 与Construct寡聚状态Molecular weight
Bromodomain adjacent to zinc finger domain protein 2B, C-terminal
查看序列
AMASIMKVYCQICRKGDNEELLLLCDGCDKGCHTYCHRPKITTIPDGDWFCPACIAKASGQTLKIKKLHVKGKKTNESKKGKKVTLTGDTEDEDSASTSSSLKRGNKDLKKRKMEENTSINLSKQESFTSVKKPKRDDSKDLALCSMILTEMETHEDAWPFLLPVNLKLVPGYKKVIKKPMDFSTIREKLSSGQYPNLETFALDVRLVFDNCETFNEDDSDIGRAGHNMRKYFEKKWTDTFKVS
proteinHomo sapiens—–—monomer分子数 127.67 kDa

实验曲线

曲线点数 / 列q range误差质量负强度点来源文件
12168[3]0.0871252–6.01453 1/nm含误差列缺失 00sasbdb/entries/56/sasda56/source/SASDA56.dat

2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)14.5nm
i0Guinier28.0
i0P(r)28.5
mwExperimental25.0kDa
mwGuinier I(0)33.0kDa
mwPorod25.0kDa
porod_volumePorod43.0nm³
rgGuinier4.0nm
rgP(r)4.2nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

3. 来源拟合与模型 Source Fits & Models

4. 来源文件索引 Source Files

5. 实验说明与论文 Experiment & Publication

6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;Not declared的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称20 mM Tris 500 mM NaCl 2 mM DTT 10 microM ZnCl2缓冲液浓度20.0 mM
pH8.0添加剂NaCl 500.000 mM, DTT 2.000 mM, ZnCl2 10.000 microM
缓冲液说明
纯度测定方法消光系数
吸收值散射对比度
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2012-09-24储存 / 测量温度10.0 / 10.0
曝光时间15.0帧数8
波长0.15样品-探测器距离2.7
光源X-ray synchrotron探测器Pilatus 1M-W
机构 / 束线DORIS III, DESY / EMBL X33 · Hamburg, Germany
q range0.087 – 6.015样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可Download文件

类别文件状态大小校验值Download与查看
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curve:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:234 个字段值
字段路径原始值
codeSASDA56
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleMolecular basis of histone tail recognition by human TIP5 PHD finger and bromodomain of the chromatin remodeling complex NoRC.
project.publication.titleMolecular basis of histone tail recognition by human TIP5 PHD finger and bromodomain of the chromatin remodeling complex NoRC.
project.publication.author_listTallant C, Valentini E, Fedorov O, Overvoorde L, Ferguson FM, Filippakopoulos P, Svergun DI, Knapp S, Ciulli A
project.publication.journalStructure
project.publication.doi10.1016/j.str.2014.10.017
project.publication.pmid25533489
project.publication.published_date2015 Jan 6
project.statusreleased
project.submitted_datenull
project.released_date2015-07-07
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experiment.instrument.detector.namePilatus 1M-W
experiment.instrument.detector.resolution0.172
experiment.instrument.nameDORIS III, DESY
experiment.instrument.cityHamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL X33
experiment.instrument.beam_geometry0.60
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
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experiment.sample.molecule[0].long_nameBromodomain adjacent to zinc finger domain protein 2B, C-terminal
experiment.sample.molecule[0].short_nameBAZ2B
experiment.sample.molecule[0].sequenceAMASIMKVYCQICRKGDNEELLLLCDGCDKGCHTYCHRPKITTIPDGDWFCPACIAKASGQTLKIKKLHV KGKKTNESKKGKKVTLTGDTEDEDSASTSSSLKRGNKDLKKRKMEENTSINLSKQESFTSVKKPKRDDSK DLALCSMILTEMETHEDAWPFLLPVNLKLVPGYKKVIKKPMDFSTIREKLSSGQYPNLETFALDVRLVFD NCETFNEDDSDIGRAGHNMRKYFEKKWTDTFKVS
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
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experiment.sample.molecule[0].complex_statenull
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experiment.sample.molecule[0].molecule_sourcebiological
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experiment.sample.buffer.concentration_unitmM
experiment.sample.buffer.commentnull
experiment.sample.buffer.additiveNaCl 500.000 mM, DTT 2.000 mM, ZnCl2 10.000 microM
experiment.sample.buffer.concentration20.0
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph8.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameBAZ2B in Tris
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameEMBL-Hamburg
experiment.contributor[0].affiliation[0].addressNotkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany
experiment.contributor[0].affiliation[0].full_nameEuropean Molecular Biology Laboratory (EMBL) - Hamburg outstation
experiment.contributor[0].affiliation[0].webpagehttp://www.embl-hamburg.de/index.php
experiment.contributor[0].contributor_nameErica
experiment.contributor[0].contributor_surnameValentini
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitmg/ml
experiment.date2012-09-24
experiment.storage_temperature10.0
experiment.cell_temperature10.0
experiment.exposure_time15.0
experiment.number_of_frames8
experiment.wavelength0.15
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last_modified2023-05-25T11:21:52.654103+02:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDA56
statussuccess
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finished_at2026-08-11T15:02:00.730692+00:00
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查看完整 summary.json 原文
{
  "code": "SASDA56",
  "status": "Published",
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查看完整 manifest.json 原文
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