SASDA58

UL26N of pseudorabies virus

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2023-06-21T07:56:39.492710+02:00

1. 样品、组分与实验条件 Sample & Experiment

样品 1 · UL26N of pseudorabies virus

浓度— – 9.83 mg/ml缓冲液 / pH50 mM Tris/HCl 0.5 M NaCl 0.25 M imidazole 5% glycerol 50 mM urea 0.2 M MgCl2 / 7.5
Experimental temperature10.0 设备 / 束线PETRA III / EMBL P12
波长0.12 nm曝光0.05 s × 20

分子组分

组分类型 / OrganismUniProt 与Construct寡聚状态Molecular weight
VP24
查看序列
MGSSHHHHHHSSGLVPRGSHMGPVYVSGYLALYDRDGGELALTREIVAAALPPAGPLPINIDHRPRCDIGAVLAVVDDDRGPFFLGVVNCPQLGAVLARAVGPDFFGDMRLSDEERLLYLLSNYLPSASLSSRRLAPGEAPDETLFAHVALCVIGRRVGTIVVYDASPEAAVAPFRQLSARARSELLARAAESPDRERVWHMSEEALTRALLSTAVNNMLLRDRWELVAARRREAGVRGHTYLQ
proteinSuid herpesvirus 1Q834171–224dimer分子数 226.608 kDa

实验曲线

曲线点数 / 列q range误差质量负强度点来源文件
11661[3]0.0679961–4.43724 1/nm含误差列缺失 0350sasbdb/entries/58/sasda58/source/SASDA58.dat

2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)10.6nm
i0Guinier2964.0
i0P(r)2982.0
mwExperimental45.0kDa
mwPorod45.0kDa
porod_volumePorod73.0nm³
rgGuinier2.62nm
rgP(r)2.69nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

3. 来源拟合与模型 Source Fits & Models

4. 来源文件索引 Source Files

5. 实验说明与论文 Experiment & Publication

6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;Not declared的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称50 mM Tris/HCl 0.5 M NaCl 0.25 M imidazole 5% glycerol 50 mM urea 0.2 M MgCl2缓冲液浓度50.0 mM
pH7.5添加剂0.5 M NaCl, 0.25 M imidazole, 5% glycerol, 50 mM urea, 0.2 M MgCl2
缓冲液说明
纯度测定方法消光系数
吸收值散射对比度
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2013-09-23储存 / 测量温度— / 10.0
曝光时间0.05帧数20
波长0.12样品-探测器距离3.1
光源X-ray synchrotron探测器Pilatus 2M
机构 / 束线PETRA III / EMBL P12 · DESY; Hamburg, Germany
q range0.068 – 4.437样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可Download文件

类别文件状态大小校验值Download与查看
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curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(6 项)
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pddf:来源记录
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sascif:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:182 个字段值
字段路径原始值
codeSASDA58
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleDimerization-Induced Allosteric Changes of the Oxyanion-Hole Loop Activate the Pseudorabies Virus Assemblin pUL26N, a Herpesvirus Serine Protease.
project.publication.titleDimerization-Induced Allosteric Changes of the Oxyanion-Hole Loop Activate the Pseudorabies Virus Assemblin pUL26N, a Herpesvirus Serine Protease.
project.publication.author_listZühlsdorf M, Werten S, Klupp BG, Palm GJ, Mettenleiter TC, Hinrichs W
project.publication.journalPLoS Pathog
project.publication.doi10.1371/journal.ppat.1005045
project.publication.pmid26161660
project.publication.published_date2015 Jul
project.statusreleased
project.submitted_date2015-04-25
project.released_date2015-07-17
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experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus 2M
experiment.instrument.detector.resolutionnull
experiment.instrument.namePETRA III
experiment.instrument.cityDESY; Hamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL P12
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameVP24
experiment.sample.molecule[0].short_nameUL26N
experiment.sample.molecule[0].sequenceMGSSHHHHHHSSGLVPRGSHMGPVYVSGYLALYDRDGGELALTREIVAAALPPAGPLPINIDHRPRCDIGAVLAVVDDDRGPFFLGVVNCPQLGAVLARAVGPDFFGDMRLSDEERLLYLLSNYLPSASLSSRRLAPGEAPDETLFAHVALCVIGRRVGTIVVYDASPEAAVAPFRQLSARARSELLARAAESPDRERVWHMSEEALTRALLSTAVNNMLLRDRWELVAARRREAGVRGHTYLQ
experiment.sample.molecule[0].organismSuid herpesvirus 1
experiment.sample.molecule[0].uniprot_codeQ83417
experiment.sample.molecule[0].uniprot_range_first1
experiment.sample.molecule[0].uniprot_range_last224
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMGPVYVSGYLALYDRDGGELALTREIVAAALPPAGPLPINIDHRPRCDIGAVLAVVDDDR GPFFLGVVNCPQLGAVLARAVGPDFFGDMRLSDEERLLYLLSNYLPSASLSSRRLAPGEA PDETLFAHVALCVIGRRVGTIVVYDASPEAAVAPFRQLSARARSELLARAAESPDRERVW HMSEEALTRALLSTAVNNMLLRDRWELVAARRREAGVRGHTYLQATMWAGLLPKSGASPA PGPSAAMAAPPSAAPGDYIFVPAAQYNQLVVNQRPAPSLESQLGAIVSAAMDRRHRRSPS PEPRPPARKRRYDDYAQDNAYYPGEAPPPASDLAAVVSSLQREISHLRAQQLRYPTPYYA PAAPPQLLPPGAVVGHPHPHHAAGALYPPMYAPQPGLHAPPPSPVAHAVPALPGLPGLQG LAAPVAHVPAQVVPQQPVVVQAQPVAVPAAAAAAPAPAPAAAAAAAAPVQAAAPAAPASA PQPPVQASVSAPADVSAGTIDASSAAVACQRGADIFVSQMMSQR
experiment.sample.molecule[0].mw26.608
experiment.sample.molecule[0].total_mw53.216
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name50 mM Tris/HCl 0.5 M NaCl 0.25 M imidazole 5% glycerol 50 mM urea 0.2 M MgCl2
experiment.sample.buffer.concentration_unitmM
experiment.sample.buffer.commentnull
experiment.sample.buffer.additive0.5 M NaCl, 0.25 M imidazole, 5% glycerol, 50 mM urea, 0.2 M MgCl2
experiment.sample.buffer.concentration50.0
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameUL26N of pseudorabies virus
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressDomstraße 11 17489 Greifswald
experiment.contributor[0].affiliation[0].full_nameErnst-Moritz-Arndt-Universität Greifswald
experiment.contributor[0].affiliation[0].webpagehttp://www.uni-greifswald.de/
experiment.contributor[0].contributor_nameSebastiaan
experiment.contributor[0].contributor_surnameWerten
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitmg/ml
experiment.date2013-09-23
experiment.storage_temperaturenull
experiment.cell_temperature10.0
experiment.exposure_time0.05
experiment.number_of_frames20
experiment.wavelength0.12
experiment.sample_detector_distance3.1
experiment.concentration_minnull
experiment.concentration_max9.83
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.068
experiment.s_max4.437
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].models[0].pdb_link[0].ext_code4V07
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fits[1].softwarenull
fits[1].chi_square_value1.196
fits[1].p_value0.58613
fits[1].fit_residual_plotSASDA58_fit2_fitresiduals_img.png
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fits[1].fit_lognull
fits[1].software_versionnull
fits[1].descriptionnull
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_versionnull
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unitnull
experimental_mw45.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw45.0
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guinier_rg_errornull
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pddf_dmax_errornull
porod_volume73.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_firstnull
guinier_point_lastnull
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDA58_datloglog_img.png
symmetrynull
last_modified2023-06-21T07:56:39.492710+02:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDA58
statussuccess
started_at2026-08-11T15:45:58.898803+00:00
finished_at2026-08-11T15:46:08.129629+00:00
source_last_modified2023-06-21T07:56:39.492710+02:00
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查看完整 summary.json 原文
{
  "code": "SASDA58",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "Dimerization-Induced Allosteric Changes of the Oxyanion-Hole Loop Activate the Pseudorabies Virus Assemblin pUL26N, a Herpesvirus Serine Protease.",
    "publication": {
      "title": "Dimerization-Induced Allosteric Changes of the Oxyanion-Hole Loop Activate the Pseudorabies Virus Assemblin pUL26N, a Herpesvirus Serine Protease.",
      "author_list": "Zühlsdorf M, Werten S, Klupp BG, Palm GJ, Mettenleiter TC, Hinrichs W",
      "journal": "PLoS Pathog",
      "doi": "10.1371/journal.ppat.1005045",
      "pmid": "26161660",
      "published_date": "2015 Jul"
    },
    "status": "released",
    "submitted_date": "2015-04-25",
    "released_date": "2015-07-17"
  },
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      "country": "Germany",
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    },
    "sample": {
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查看完整 manifest.json 原文
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