SASDA66

BAZ2B-18mer complex in Tris

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2023-05-25T11:21:52.654103+02:00

1. 样品、组分与实验条件 Sample & Experiment

样品 1 · BAZ2B-18mer complex in Tris

浓度— – 2.75 缓冲液 / pH20 mM Tris 500 mM NaCl 2 mM DTT 10 microM ZnCl2 / 8.0
实验温度10.0 设备 / 束线DORIS III, DESY / EMBL X33
波长0.15 nm曝光15.0 s × 8

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Bromodomain adjacent to zinc finger domain protein 2B, C-terminal
查看序列
AMASIMKVYCQICRKGDNEELLLLCDGCDKGCHTYCHRPKITTIPDGDWFCPACIAKASGQTLKIKKLHVKGKKTNESKKGKKVTLTGDTEDEDSASTSSSLKRGNKDLKKRKMEENTSINLSKQESFTSVKKPKRDDSKDLALCSMILTEMETHEDAWPFLLPVNLKLVPGYKKVIKKPMDFSTIREKLSSGQYPNLETFALDVRLVFDNCETFNEDDSDIGRAGHNMRKYFEKKWTDTFKVS
proteinHomo sapiens—–—monomer分子数 127.67 kDa
H3Kac9Kac14
查看序列
ARTKQTARKSTGGKAPRK
proteinHomo sapiens—–—monomer分子数 14.7 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
12168[3]0.0871252–6.01453 1/nm含误差列缺失 03sasbdb/entries/66/sasda66/source/SASDA66.dat

2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)16.0nm
i0Guinier37.5
i0P(r)37.5
mwExperimental31.0kDa
mwGuinier I(0)45.0kDa
mwPorod31.0kDa
porod_volumePorod52.0nm³
rgGuinier4.2nm
rgP(r)4.5nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

3. 来源拟合与模型 Source Fits & Models

该条目没有来源拟合记录。

4. 来源文件索引 Source Files

5. 实验说明与论文 Experiment & Publication

6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称20 mM Tris 500 mM NaCl 2 mM DTT 10 microM ZnCl2缓冲液浓度20.0 mM
pH8.0添加剂NaCl 500.000 mM, DTT 2.000 mM, ZnCl2 10.000 microM
缓冲液说明
纯度测定方法消光系数
吸收值散射对比度
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2012-09-24储存 / 测量温度10.0 / 10.0
曝光时间15.0帧数8
波长0.15样品-探测器距离2.7
光源X-ray synchrotron探测器Pilatus 1M-W
机构 / 束线DORIS III, DESY / EMBL X33 · Hamburg, Germany
q 范围0.087 – 6.015样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
curvesasbdb/entries/66/sasda66/source/SASDA66.datdownloaded9875222579790993251ff57750be914975bf25d5cfe6544e18a27a8fc401fa9972efb下载查看原文件源站
full_entry_zipsasbdb/entries/66/sasda66/source/SASDA66.zipdownloaded3543457582968cdde74f3d6b1d555cff137c720facdd1eedd40a63fc84c8b564e4ade下载查看原文件源站
pddfsasbdb/entries/66/sasda66/source/SASDA66.outdownloaded37329b37742e5941bb9ed6c33febf80a6464eea187497ca1dc6349d125e7d174853d5下载查看原文件源站
sascifsasbdb/entries/66/sasda66/source/SASDA66.sascifdownloaded149309788b9bc8345dfc0e96386888967d60f2f909389447fba919b7fb22f80ca51863下载查看原文件源站
summarysasbdb/entries/66/sasda66/source/summary.jsondownloaded63867e8fcb42035022509303bc1af284c19465bad6c9d782bc33fb65aa921c9dc6e2下载查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(2 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:151 个字段值
字段路径原始值
codeSASDA66
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleMolecular basis of histone tail recognition by human TIP5 PHD finger and bromodomain of the chromatin remodeling complex NoRC.
project.publication.titleMolecular basis of histone tail recognition by human TIP5 PHD finger and bromodomain of the chromatin remodeling complex NoRC.
project.publication.author_listTallant C, Valentini E, Fedorov O, Overvoorde L, Ferguson FM, Filippakopoulos P, Svergun DI, Knapp S, Ciulli A
project.publication.journalStructure
project.publication.doi10.1016/j.str.2014.10.017
project.publication.pmid25533489
project.publication.published_date2015 Jan 6
project.statusreleased
project.submitted_datenull
project.released_date2015-07-07
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sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDA66.sascif
experiment.instrument.detector.typeCustom-built
experiment.instrument.detector.namePilatus 1M-W
experiment.instrument.detector.resolution0.172
experiment.instrument.nameDORIS III, DESY
experiment.instrument.cityHamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL X33
experiment.instrument.beam_geometry0.60
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameBromodomain adjacent to zinc finger domain protein 2B, C-terminal
experiment.sample.molecule[0].short_nameBAZ2B
experiment.sample.molecule[0].sequenceAMASIMKVYCQICRKGDNEELLLLCDGCDKGCHTYCHRPKITTIPDGDWFCPACIAKASGQTLKIKKLHV KGKKTNESKKGKKVTLTGDTEDEDSASTSSSLKRGNKDLKKRKMEENTSINLSKQESFTSVKKPKRDDSK DLALCSMILTEMETHEDAWPFLLPVNLKLVPGYKKVIKKPMDFSTIREKLSSGQYPNLETFALDVRLVFD NCETFNEDDSDIGRAGHNMRKYFEKKWTDTFKVS
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequencenull
experiment.sample.molecule[0].mw27.67
experiment.sample.molecule[0].total_mw27.67
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_statenull
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.molecule[1].long_nameH3Kac9Kac14
experiment.sample.molecule[1].short_namepeptide 18mer
experiment.sample.molecule[1].sequenceARTKQTARKSTGGKAPRK
experiment.sample.molecule[1].organismHomo sapiens
experiment.sample.molecule[1].uniprot_codenull
experiment.sample.molecule[1].uniprot_range_firstnull
experiment.sample.molecule[1].uniprot_range_lastnull
experiment.sample.molecule[1].oligomerizationmonomer
experiment.sample.molecule[1].molecular_typeprotein
experiment.sample.molecule[1].uniprot_sequencenull
experiment.sample.molecule[1].mw4.7
experiment.sample.molecule[1].total_mw4.7
experiment.sample.molecule[1].number_molecules1
experiment.sample.molecule[1].complex_statenull
experiment.sample.molecule[1].deuterationnull
experiment.sample.molecule[1].molecule_sourcebiological
experiment.sample.molecule[1].molecule_descriptionnull
experiment.sample.buffer.name20 mM Tris 500 mM NaCl 2 mM DTT 10 microM ZnCl2
experiment.sample.buffer.concentration_unitmM
experiment.sample.buffer.commentnull
experiment.sample.buffer.additiveNaCl 500.000 mM, DTT 2.000 mM, ZnCl2 10.000 microM
experiment.sample.buffer.concentration20.0
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph8.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameBAZ2B-18mer complex in Tris
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameEMBL-Hamburg
experiment.contributor[0].affiliation[0].addressNotkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany
experiment.contributor[0].affiliation[0].full_nameEuropean Molecular Biology Laboratory (EMBL) - Hamburg outstation
experiment.contributor[0].affiliation[0].webpagehttp://www.embl-hamburg.de/index.php
experiment.contributor[0].contributor_nameErica
experiment.contributor[0].contributor_surnameValentini
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2012-09-24
experiment.storage_temperature10.0
experiment.cell_temperature10.0
experiment.exposure_time15.0
experiment.number_of_frames8
experiment.wavelength0.15
experiment.sample_detector_distance2.7
experiment.concentration_minnull
experiment.concentration_max2.75
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.087
experiment.s_max6.015
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[]
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_versionnull
i0_calibration_standardBSA
descriptionnull
experiment_descriptionnull
tags[0]X33
intensity_unitnull
experimental_mw31.0
experimental_mw_errornull
guinier_i0_mw45.0
guinier_i0_mw_errornull
porod_mw31.0
porod_mw_errornull
pddf_i037.5
pddf_i0_errornull
guinier_i037.5
guinier_i0_errornull
pddf_rg4.5
pddf_rg_errornull
guinier_rg4.2
guinier_rg_errornull
pddf_dmax16.0
pddf_dmax_errornull
porod_volume52.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first33
guinier_point_last84
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDA66_datloglog_img.png
symmetrynull
last_modified2023-05-25T11:21:52.654103+02:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDA66
statussuccess
started_at2026-08-11T15:02:27.879460+00:00
finished_at2026-08-11T15:02:36.442412+00:00
source_last_modified2023-05-25T11:21:52.654103+02:00
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查看完整 summary.json 原文
{
  "code": "SASDA66",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "Molecular basis of histone tail recognition by human TIP5 PHD finger and bromodomain of the chromatin remodeling complex NoRC.",
    "publication": {
      "title": "Molecular basis of histone tail recognition by human TIP5 PHD finger and bromodomain of the chromatin remodeling complex NoRC.",
      "author_list": "Tallant C, Valentini E, Fedorov O, Overvoorde L, Ferguson FM, Filippakopoulos P, Svergun DI, Knapp S, Ciulli A",
      "journal": "Structure",
      "doi": "10.1016/j.str.2014.10.017",
      "pmid": "25533489",
      "published_date": "2015 Jan 6"
    },
    "status": "released",
    "submitted_date": null,
    "released_date": "2015-07-07"
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        "concentration_unit": "mM",
        "comment": null,
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        "concentration": 20.0,
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}
查看完整 manifest.json 原文
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