SASDA74

RNA Aptamer

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2023-05-25T11:22:16.556436+02:00

1. 样品、组分与实验条件 Sample & Experiment

样品 1 · RNA Aptamer

浓度— – 0.5 mg/ml缓冲液 / pHHepes / 7.4
Experimental temperature15.0 设备 / 束线DORIS III, DESY / EMBL X33
波长— nm曝光120.0 s × 2

分子组分

组分类型 / OrganismUniProt 与Construct寡聚状态Molecular weight
SRB2m
查看序列
GGAACCUCGCUUCGGCGAUGAUGGAGAGGCGCAAGGUUAACCGCCUCAGGUUCC
RNA—–—dimer分子数 216.55 kDa

实验曲线

曲线点数 / 列q range误差质量负强度点来源文件
12087[3]0.242538–5.07169 1/nm含误差列缺失 037sasbdb/entries/74/sasda74/source/SASDA74.dat

2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)8.5nm
i0Guinier207.0
i0P(r)210.0
mwExperimental30.0kDa
porod_volumePorod31.0nm³
rgGuinier2.4nm
rgP(r)2.5nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

3. 来源拟合与模型 Source Fits & Models

4. 来源文件索引 Source Files

5. 实验说明与论文 Experiment & Publication

6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;Not declared的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称Hepes缓冲液浓度
pH7.4添加剂
缓冲液说明
纯度测定方法消光系数
吸收值散射对比度
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2007-10-24储存 / 测量温度15.0 / 15.0
曝光时间120.0帧数2
波长样品-探测器距离
光源X-ray synchrotron探测器MAR 345 Image Plate
机构 / 束线DORIS III, DESY / EMBL X33 · Hamburg, Germany
q range0.243 – 5.072样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图

可Download文件

类别文件状态大小校验值Download与查看
curvesasbdb/entries/74/sasda74/source/SASDA74.datdownloaded954523ec689ebc9df19ab53f3bea3ca227d8869421021fa3294a218c72e60d166ef0bDownload查看原文件源站
full_entry_zipsasbdb/entries/74/sasda74/source/SASDA74.zipdownloaded50120707041443fd82f5ea05f151a8ab2aeef86fa9f55de743e758736a65793bac8ebDownload查看原文件源站
pddfsasbdb/entries/74/sasda74/source/SASDA74.outnot_listedDownload查看原文件
sascifsasbdb/entries/74/sasda74/source/SASDA74.sascifdownloaded24188406a159f15e173896c16887d994cc97aa69236f21a221b668c6805764f328cbf8Download查看原文件源站
summarysasbdb/entries/74/sasda74/source/summary.jsondownloaded618197ff757a2b4811bf961e6a868f276d203c8fe1c5997febed45458b3e8fc5519fDownload查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(3 项)
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pddf:来源记录
{
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  "role": "pddf",
  "status": "not_listed"
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:156 个字段值
字段路径原始值
codeSASDA74
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleCharacterization of a fluorophore binding RNA aptamer by fluorescence correlation spectroscopy and small angle X-ray scattering.
project.publication.titleCharacterization of a fluorophore binding RNA aptamer by fluorescence correlation spectroscopy and small angle X-ray scattering.
project.publication.author_listWerner A, Konarev PV, Svergun DI, Hahn U
project.publication.journalAnal Biochem
project.publication.doi10.1016/j.ab.2009.03.018
project.publication.pmid19303859
project.publication.published_date2009 Jun 1
project.statusreleased
project.submitted_datenull
project.released_date2015-07-07
pddf_datanull
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDA74.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDA74_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDA74_kratky_img.png
pddf_plotnull
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDA74_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDA74.sascif
experiment.instrument.detector.typenull
experiment.instrument.detector.nameMAR 345 Image Plate
experiment.instrument.detector.resolutionnull
experiment.instrument.nameDORIS III, DESY
experiment.instrument.cityHamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL X33
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameSRB2m
experiment.sample.molecule[0].short_nameSRB2m
experiment.sample.molecule[0].sequenceGGAACCUCGCUUCGGCGAUGAUGGAGAGGC GCAAGGUUAACCGCCUCAGGUUCC
experiment.sample.molecule[0].organismnull
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeRNA
experiment.sample.molecule[0].uniprot_sequencenull
experiment.sample.molecule[0].mw16.55
experiment.sample.molecule[0].total_mw33.1
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_statenull
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.nameHepes
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.4
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameRNA Aptamer
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameEMBL-Hamburg
experiment.contributor[0].affiliation[0].addressNotkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany
experiment.contributor[0].affiliation[0].full_nameEuropean Molecular Biology Laboratory (EMBL) - Hamburg outstation
experiment.contributor[0].affiliation[0].webpagehttp://www.embl-hamburg.de/index.php
experiment.contributor[0].contributor_namePetr
experiment.contributor[0].contributor_surnameKonarev
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitmg/ml
experiment.date2007-10-24
experiment.storage_temperature15.0
experiment.cell_temperature15.0
experiment.exposure_time120.0
experiment.number_of_frames2
experiment.wavelengthnull
experiment.sample_detector_distancenull
experiment.concentration_minnull
experiment.concentration_max0.5
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.243
experiment.s_max5.072
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[0].models[0].model_plothttps://www.sasbdb.org/media/pdb_file/images/SASDA74_fit1_model1_img.png
fits[0].models[0].softwareDAMMIN
fits[0].models[0].pdb_link[]
fits[0].models[0].model_titlenull
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fits[0].models[0].model_mwnull
fits[0].models[0].bead_radiusnull
fits[0].models[0].lognull
fits[0].models[0].symmetry
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fits[0].models[0].user88
fits[0].fit_unit1/A
fits[0].fit_plothttps://www.sasbdb.org/media/fitting_files/scattering_plots/SASDA74_fit1_fit_img.png
fits[0].softwarenull
fits[0].chi_square_value4.888521
fits[0].p_value0.0
fits[0].fit_residual_plotSASDA74_fit1_fitresiduals_img.png
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fits[0].fit_lognull
fits[0].software_versionnull
fits[0].descriptionnull
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_versionGNOM
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[0]X33
intensity_unitnull
experimental_mw30.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mwnull
porod_mw_errornull
pddf_i0210.0
pddf_i0_errornull
guinier_i0207.0
guinier_i0_errornull
pddf_rg2.5
pddf_rg_errornull
guinier_rg2.4
guinier_rg_errornull
pddf_dmax8.5
pddf_dmax_errornull
porod_volume31.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_firstnull
guinier_point_lastnull
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDA74_datloglog_img.png
symmetrynull
last_modified2023-05-25T11:22:16.556436+02:00
bragg_peak[]
manifest.json:33 个字段值
字段路径原始值
codeSASDA74
statussuccess
started_at2026-08-11T14:15:08.003969+00:00
finished_at2026-08-11T14:15:14.973319+00:00
source_last_modified2023-05-25T11:22:16.556436+02:00
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查看完整 summary.json 原文
{
  "code": "SASDA74",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "Characterization of a fluorophore binding RNA aptamer by fluorescence correlation spectroscopy and small angle X-ray scattering.",
    "publication": {
      "title": "Characterization of a fluorophore binding RNA aptamer by fluorescence correlation spectroscopy and small angle X-ray scattering.",
      "author_list": "Werner A, Konarev PV, Svergun DI, Hahn U",
      "journal": "Anal Biochem",
      "doi": "10.1016/j.ab.2009.03.018",
      "pmid": "19303859",
      "published_date": "2009 Jun 1"
    },
    "status": "released",
    "submitted_date": null,
    "released_date": "2015-07-07"
  },
  "pddf_data": null,
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDA74.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDA74_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDA74_kratky_img.png",
  "pddf_plot": null,
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDA74_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDA74.sascif",
  "experiment": {
    "instrument": {
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        "name": "MAR 345 Image Plate",
        "resolution": null
      },
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      "city": "Hamburg",
      "country": "Germany",
      "beamline_name": "EMBL X33",
      "beam_geometry": null,
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      "line_collimation_integrationwidth": null,
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      "beam_profile_ah": null,
      "beam_profile_al": null
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    "sample": {
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          "short_name": "SRB2m",
          "sequence": "GGAACCUCGCUUCGGCGAUGAUGGAGAGGC\r\nGCAAGGUUAACCGCCUCAGGUUCC",
          "organism": null,
          "uniprot_code": null,
          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "dimer",
          "molecular_type": "RNA",
          "uniprot_sequence": null,
          "mw": 16.55,
          "total_mw": 33.1,
          "number_molecules": 2,
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        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.4,
        "deuteration": null
      },
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      "name": "RNA Aptamer",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
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  "tags": [
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  "porod_mw_error": null,
  "pddf_i0": 210.0,
  "pddf_i0_error": null,
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  "guinier_i0_error": null,
  "pddf_rg": 2.5,
  "pddf_rg_error": null,
  "guinier_rg": 2.4,
  "guinier_rg_error": null,
  "pddf_dmax": 8.5,
  "pddf_dmax_error": null,
  "porod_volume": 31.0,
  "porod_volume_error": null,
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  "estimated_volume_error": null,
  "guinier_point_first": null,
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  "symmetry": null,
  "last_modified": "2023-05-25T11:22:16.556436+02:00",
  "bragg_peak": []
}
查看完整 manifest.json 原文
{
  "code": "SASDA74",
  "status": "success",
  "started_at": "2026-08-11T14:15:08.003969+00:00",
  "finished_at": "2026-08-11T14:15:14.973319+00:00",
  "source_last_modified": "2023-05-25T11:22:16.556436+02:00",
  "files": [
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