SASDA78

Carbonic Anhydrase in Tris/HCl

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2023-05-25T11:22:16.556436+02:00

1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Carbonic Anhydrase in Tris/HCl

浓度— – 5.1 mg/ml缓冲液 / pH100 mM Tris/HCl 100 mM NaCl / 7.5
Experimental temperature20.0 设备 / 束线DORIS III, DESY / EMBL X33
波长— nm曝光15.0 s × 8

分子组分

组分类型 / OrganismUniProt 与Construct寡聚状态Molecular weight
Carbonic anhydrase 2
查看序列
MSHHWGYGKHNGPEHWHKDFPIANGERQSPVDIDTKAVVQDPALKPLALVYGEATSRRMVNNGHSFNVEYDDSQDKAVLKDGPLTGTYRLVQFHFHWGSSDDQGSEHTVDRKKYAAELHLVHWNTKYGDFGTAAQQPDGLAVVGVFLKVGDANPALQKVLDALDSIKTKGKSTDFPNFDPGSLLPNVLDYWTYPGSLTTPPLLESVTWIVLKEPISVSSQQMLKFRTLNFNAEGEPELLMLANWRPAQPLKNRQVRGFPK
proteinBos taurusP00921—–—monomer分子数 129.113 kDa

实验曲线

曲线点数 / 列q range误差质量负强度点来源文件
12135[3]0.0810267–4.95628 1/nm含误差列缺失 01sasbdb/entries/78/sasda78/source/SASDA78.dat

2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)6.0nm
i0Guinier119.52
i0P(r)112.0
mwExperimental33.1kDa
mwGuinier I(0)33.1kDa
rgGuinier2.06nm
rgP(r)1.92nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

3. 来源拟合与模型 Source Fits & Models

4. 来源文件索引 Source Files

5. 实验说明与论文 Experiment & Publication

6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;Not declared的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称100 mM Tris/HCl 100 mM NaCl缓冲液浓度100.0 mM
pH7.5添加剂100 mM NaCl
缓冲液说明
纯度测定方法消光系数
吸收值散射对比度
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2006-05-19储存 / 测量温度— / 20.0
曝光时间15.0帧数8
波长样品-探测器距离
光源X-ray synchrotron探测器MAR 345 Image Plate
机构 / 束线DORIS III, DESY / EMBL X33 · Hamburg, Germany
q range0.081 – 4.956样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可Download文件

类别文件状态大小校验值Download与查看
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curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:163 个字段值
字段路径原始值
codeSASDA78
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleAccuracy of molecular mass determination of proteins in solution by small-angle X-ray scattering
project.publication.titleAccuracy of molecular mass determination of proteins in solution by small-angle X-ray scattering
project.publication.author_listMylonas E, Svergun D
project.publication.journalJournal of Applied Crystallography
project.publication.doi10.1107/S002188980700252X
project.publication.pmidnull
project.publication.published_date2007 Feb 17
project.statusreleased
project.submitted_date2015-07-06
project.released_date2015-07-07
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experiment.instrument.cityHamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL X33
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experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
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experiment.instrument.xray_energynull
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experiment.sample.molecule[0].long_nameCarbonic anhydrase 2
experiment.sample.molecule[0].short_namenull
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experiment.sample.molecule[0].organismBos taurus
experiment.sample.molecule[0].uniprot_codeP00921
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationmonomer
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experiment.sample.molecule[0].total_mw29.113
experiment.sample.molecule[0].number_molecules1
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experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name100 mM Tris/HCl 100 mM NaCl
experiment.sample.buffer.concentration_unitmM
experiment.sample.buffer.commentnull
experiment.sample.buffer.additive100 mM NaCl
experiment.sample.buffer.concentration100.0
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameCarbonic Anhydrase in Tris/HCl
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameEMBL-Hamburg
experiment.contributor[0].affiliation[0].addressNotkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany
experiment.contributor[0].affiliation[0].full_nameEuropean Molecular Biology Laboratory (EMBL) - Hamburg outstation
experiment.contributor[0].affiliation[0].webpagehttp://www.embl-hamburg.de/index.php
experiment.contributor[0].affiliation[1].short_nameFORTH
experiment.contributor[0].affiliation[1].addressHeraklion, Greece
experiment.contributor[0].affiliation[1].full_nameFoundation for Research and Technology-Hellas
experiment.contributor[0].affiliation[1].webpagehttp://www.forth.gr/index.php?l=e
experiment.contributor[0].contributor_nameEfstratios
experiment.contributor[0].contributor_surnameMylonas
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitmg/ml
experiment.date2006-05-19
experiment.storage_temperaturenull
experiment.cell_temperature20.0
experiment.exposure_time15.0
experiment.number_of_frames8
experiment.wavelengthnull
experiment.sample_detector_distancenull
experiment.concentration_minnull
experiment.concentration_max5.1
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.081
experiment.s_max4.956
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].models[0].softwarePDB (Protein Data Bank)
fits[0].models[0].pdb_link[0].pdb_code1v9e
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pddf_software_versionnull
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[0]X33
intensity_unitnull
experimental_mw33.1
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porod_mwnull
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pddf_rg_errornull
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guinier_rg_errornull
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guinier_point_last232
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDA78_datloglog_img.png
symmetrynull
last_modified2023-05-25T11:22:16.556436+02:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDA78
statussuccess
started_at2026-08-11T16:33:11.933071+00:00
finished_at2026-08-11T16:33:21.854695+00:00
source_last_modified2023-05-25T11:22:16.556436+02:00
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查看完整 summary.json 原文
{
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  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "Accuracy of molecular mass determination of proteins in solution by small-angle X-ray scattering",
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      "author_list": "Mylonas E, Svergun D",
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      "doi": "10.1107/S002188980700252X",
      "pmid": null,
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查看完整 manifest.json 原文
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