SASDAA5

CHD4 (AH)

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Extrapolated to infinite dilution 最后更新:2023-05-25T11:21:52.654103+02:00

The ATPase CHD4 mediates nucleosome remodeling by the NuRD (nucleosome remodeling and deacetylase) complex. The NuRD complex serves as a crucial epigenetic regulator of cell differentiation, proliferation, and hematopoietic development by coupling the deacetylation and demethylation of histones, nucleosome mobilization, and the recruitment of transcription factors. The three dimensional small-angle X-ray scattering model of CHD4 helps to define its interdomain interactions, with cross linking and limited proteolysis studies used to validate the model. Functional and binding assays suggest a regulatory role for the PHD and chromo domains.

1. 样品、组分与实验条件 Sample & Experiment

样品 1 · CHD4 (AH)

浓度1.4 – 8.6 缓冲液 / pH50 mM HEPES 50 mM KCl / 7.5
Experimental temperature10.0 设备 / 束线DORIS III, DESY / EMBL X33
波长0.15 nm曝光15.0 s × 8

分子组分

组分类型 / OrganismUniProt 与Construct寡聚状态Molecular weight
Human Chromatin Remodeler CHD4 (685-1233)
查看序列
MPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATD
proteinHomo sapiens—–—monomer分子数 162.8 kDa

实验曲线

曲线点数 / 列q range误差质量负强度点来源文件
12129[3]0.198457–6.01554 1/nm含误差列缺失 019sasbdb/entries/a5/sasdaa5/source/SASDAA5.dat

2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)14.5nm
i0Guinier45.63
mwExperimental52.0kDa
mwGuinier I(0)52.0kDa
rgGuinier4.0nm
rgP(r)4.2nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

3. 来源拟合与模型 Source Fits & Models

4. 来源文件索引 Source Files

5. 实验说明与论文 Experiment & Publication

6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;Not declared的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称50 mM HEPES 50 mM KCl缓冲液浓度50.0 mM
pH7.5添加剂KCl 50.000 mM
缓冲液说明4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid
纯度测定方法消光系数
吸收值散射对比度
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2010-11-13储存 / 测量温度10.0 / 10.0
曝光时间15.0帧数8
波长0.15样品-探测器距离2.7
光源X-ray synchrotron探测器Pilatus 1M-W
机构 / 束线DORIS III, DESY / EMBL X33 · Hamburg, Germany
q range0.198 – 6.016样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可Download文件

类别文件状态大小校验值Download与查看
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curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:156 个字段值
字段路径原始值
codeSASDAA5
statusPublished
type_of_curveExtrapolated to infinite dilution
angular_unit1/nm
project.titleThe PHD and chromo domains regulate the ATPase activity of the human chromatin remodeler CHD4.
project.publication.titleThe PHD and chromo domains regulate the ATPase activity of the human chromatin remodeler CHD4.
project.publication.author_listWatson AA, Mahajan P, Mertens HD, Deery MJ, Zhang W, Pham P, Du X, Bartke T, Zhang W, Edlich C, Berridge G, Chen Y, Burgess-Brown NA, Kouzarides T, Wiechens N, Owen-Hughes T, Svergun DI, Gileadi O, Laue ED
project.publication.journalJ Mol Biol
project.publication.doi10.1016/j.jmb.2012.04.031
project.publication.pmid22575888
project.publication.published_date2012 Sep 7
project.statusreleased
project.submitted_datenull
project.released_date2015-07-07
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experiment.instrument.detector.typeCustom-built
experiment.instrument.detector.namePilatus 1M-W
experiment.instrument.detector.resolution0.172
experiment.instrument.nameDORIS III, DESY
experiment.instrument.cityHamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL X33
experiment.instrument.beam_geometry0.60
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
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experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameHuman Chromatin Remodeler CHD4 (685-1233)
experiment.sample.molecule[0].short_nameCHD4 (AH)
experiment.sample.molecule[0].sequenceMPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTV QTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKAS RMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEE LFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYK YILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLK EGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATAD TVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQEL DDILKFGTEELFKDEATD
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequencenull
experiment.sample.molecule[0].mw62.8
experiment.sample.molecule[0].total_mw62.8
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_statenull
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name50 mM HEPES 50 mM KCl
experiment.sample.buffer.concentration_unitmM
experiment.sample.buffer.comment4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid
experiment.sample.buffer.additiveKCl 50.000 mM
experiment.sample.buffer.concentration50.0
experiment.sample.buffer.pka7.0
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameCHD4 (AH)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameEMBL-Hamburg
experiment.contributor[0].affiliation[0].addressNotkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany
experiment.contributor[0].affiliation[0].full_nameEuropean Molecular Biology Laboratory (EMBL) - Hamburg outstation
experiment.contributor[0].affiliation[0].webpagehttp://www.embl-hamburg.de/index.php
experiment.contributor[0].contributor_nameHaydyn
experiment.contributor[0].contributor_surnameMertens
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2010-11-13
experiment.storage_temperature10.0
experiment.cell_temperature10.0
experiment.exposure_time15.0
experiment.number_of_frames8
experiment.wavelength0.15
experiment.sample_detector_distance2.7
experiment.concentration_min1.4
experiment.concentration_max8.6
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.198
experiment.s_max6.016
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].models[0].softwareDAMMIF
fits[0].models[0].pdb_link[]
fits[0].models[0].model_titlenull
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fits[0].models[0].user12
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fits[0].softwarenull
fits[0].chi_square_value1.592644
fits[0].p_value0.000135
fits[0].fit_residual_plotSASDAA5_fit1_fitresiduals_img.png
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fits[0].fit_lognull
fits[0].software_versionnull
fits[0].descriptionnull
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_versionnull
i0_calibration_standardBSA
descriptionThe ATPase CHD4 mediates nucleosome remodeling by the NuRD (nucleosome remodeling and deacetylase) complex. The NuRD complex serves as a crucial epigenetic regulator of cell differentiation, proliferation, and hematopoietic development by coupling the deacetylation and demethylation of histones, nucleosome mobilization, and the recruitment of transcription factors. The three dimensional small-angle X-ray scattering model of CHD4 helps to define its interdomain interactions, with cross linking and limited proteolysis studies used to validate the model. Functional and binding assays suggest a regulatory role for the PHD and chromo domains.
experiment_descriptionnull
tags[0]X33
intensity_unitnull
experimental_mw52.0
experimental_mw_errornull
guinier_i0_mw52.0
guinier_i0_mw_errornull
porod_mwnull
porod_mw_errornull
pddf_i0null
pddf_i0_errornull
guinier_i045.63
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pddf_rg_errornull
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guinier_rg_errornull
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pddf_dmax_errornull
porod_volumenull
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first1
guinier_point_last52
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDAA5_datloglog_img.png
symmetrynull
last_modified2023-05-25T11:21:52.654103+02:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDAA5
statussuccess
started_at2026-08-11T14:42:59.587569+00:00
finished_at2026-08-11T14:43:08.505220+00:00
source_last_modified2023-05-25T11:21:52.654103+02:00
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查看完整 summary.json 原文
{
  "code": "SASDAA5",
  "status": "Published",
  "type_of_curve": "Extrapolated to infinite dilution",
  "angular_unit": "1/nm",
  "project": {
    "title": "The PHD and chromo domains regulate the ATPase activity of the human chromatin remodeler CHD4.",
    "publication": {
      "title": "The PHD and chromo domains regulate the ATPase activity of the human chromatin remodeler CHD4.",
      "author_list": "Watson AA, Mahajan P, Mertens HD, Deery MJ, Zhang W, Pham P, Du X, Bartke T, Zhang W, Edlich C, Berridge G, Chen Y, Burgess-Brown NA, Kouzarides T, Wiechens N, Owen-Hughes T, Svergun DI, Gileadi O, Laue ED",
      "journal": "J Mol Biol",
      "doi": "10.1016/j.jmb.2012.04.031",
      "pmid": "22575888",
      "published_date": "2012 Sep 7"
    },
    "status": "released",
    "submitted_date": null,
    "released_date": "2015-07-07"
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      "country": "Germany",
      "beamline_name": "EMBL X33",
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      "beam_profile_ah": null,
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    "sample": {
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            "short_name": "EMBL-Hamburg",
            "address": "Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany",
            "full_name": "European Molecular Biology Laboratory (EMBL) - Hamburg outstation",
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}
查看完整 manifest.json 原文
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