SASDAD4

Full length GtBP3

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2023-05-25T11:22:16.556436+02:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Full length GtBP3

Concentration2.0 – 8.0 Buffer / pH25 mM Tris/HCl 250 mM NaCl / 8.0
Experimental temperature10.0 Facility / beamlineDORIS III, DESY / EMBL X33
Wavelength— nmExposure120.0 s × 2

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
full length CtBP3
View sequence
MGHHHHHHMSGVRPPIMNGPMHPRPLVALLDGRDCTVEMPILKDVATVAFCDAQSTQEIHEKVLNEAVGALMYHTITLTREDLEKFKALRIIVRIGSGFDNIDIKSAGDLGIAVCNVPAASVEETADSTLCHILNLYRRTTWLHQALREGTRVQSVEQIREVASGAARIRGETLGIIGLGRVGQAVALRAKAFGFNVLFYDPYLSDGIERALGLQRVSTLQDLLFHSDCVTLHCGLNEHNHHLINDFTVKQMRQGAFLVNTARGGLMDEKALAQALKEGRIRGAALDVHESEPFSFSQGPLKDAPNLICTPHAAWYSEQASIEMREEAAREIRRAITGRIPDSLKNCVNKDHLTAATHWASMDPAVVHPELNGAAYSRYPPGVVSVAPTGIPAAVEGIVPSAMSLSHGLPPVAHPPHAPSPGQTVKPEADRDHTTDQL
protein———–—tetramerMolecule count 442.0 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
1916[3]0.157028–3.39429 1/nmHas uncertainty columnMissing 0147sasbdb/entries/d4/sasdad4/source/SASDAD4.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)19.0—nm
i0Guinier291.7——
i0P(r)288.0——
mwExperimental170.0—kDa
porod_volumePorod330.0—nm³
rgGuinier5.1—nm
rgP(r)5.23—nm

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name25 mM Tris/HCl 250 mM NaClBuffer concentration—
pH8.0Additives250 mM NaCl
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2004-11-18Storage / measurement temperature10.0 / 10.0
Exposure time120.0Frames2
Wavelength—Sample-to-detector distance—
SourceX-ray synchrotronDetectorMAR 345 Image Plate
Facility / beamlineDORIS III, DESY / EMBL X33 · Hamburg, Germany
q range0.157 – 3.394Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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pddf:source record
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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:179 field values
Field pathRaw value
codeSASDAD4
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleThe C-terminal domain of the transcriptional corepressor CtBP is intrinsically unstructured.
project.publication.titleThe C-terminal domain of the transcriptional corepressor CtBP is intrinsically unstructured.
project.publication.author_listNardini M, Svergun D, Konarev PV, Spanò S, Fasano M, Bracco C, Pesce A, Donadini A, Cericola C, Secundo F, Luini A, Corda D, Bolognesi M
project.publication.journalProtein Sci
project.publication.doi10.1110/ps.062115406
project.publication.pmid16597837
project.publication.published_date2006 May
project.statusreleased
project.submitted_datenull
project.released_date2015-07-07
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experiment.instrument.detector.typenull
experiment.instrument.detector.nameMAR 345 Image Plate
experiment.instrument.detector.resolutionnull
experiment.instrument.nameDORIS III, DESY
experiment.instrument.cityHamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL X33
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
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experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_namefull length CtBP3
experiment.sample.molecule[0].short_namefull CtBP3
experiment.sample.molecule[0].sequenceMGHHHHHHMSGVRPPIMNGPMHPRPLVALLDGRDCTVEMPILKDVATVAFCDA QSTQEIHEKVLNEAVGALMYHTITLTREDLEKFKALRIIVRIGSGFDNIDIKSAGDLGI AVCNVPAASVEETADSTLCHILNLYRRTTWLHQALREGTRVQSVEQIREVASGAARIRG ETLGIIGLGRVGQAVALRAKAFGFNVLFYDPYLSDGIERALGLQRVSTLQDLLFHSDCV TLHCGLNEHNHHLINDFTVKQMRQGAFLVNTARGGLMDEKALAQALKEGRIRGAALDVH ESEPFSFSQGPLKDAPNLICTPHAAWYSEQASIEMREEAAREIRRAITGRIPDSLKNCV NKDHLTAATHWASMDPAVVHPELNGAAYSRYPPGVVSVAPTGIPAAVEGIVPSAMSLSH GLPPVAHPPHAPSPGQTVKPEADRDHTTDQL
experiment.sample.molecule[0].organismnull
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationtetramer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequencenull
experiment.sample.molecule[0].mw42.0
experiment.sample.molecule[0].total_mw170.0
experiment.sample.molecule[0].number_molecules4
experiment.sample.molecule[0].complex_statenull
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name25 mM Tris/HCl 250 mM NaCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additive250 mM NaCl
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph8.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameFull length GtBP3
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameEMBL-Hamburg
experiment.contributor[0].affiliation[0].addressNotkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany
experiment.contributor[0].affiliation[0].full_nameEuropean Molecular Biology Laboratory (EMBL) - Hamburg outstation
experiment.contributor[0].affiliation[0].webpagehttp://www.embl-hamburg.de/index.php
experiment.contributor[0].contributor_namePetr
experiment.contributor[0].contributor_surnameKonarev
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2004-11-18
experiment.storage_temperature10.0
experiment.cell_temperature10.0
experiment.exposure_time120.0
experiment.number_of_frames2
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experiment.concentration_min2.0
experiment.concentration_max8.0
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.157
experiment.s_max3.394
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].p_value0.000104
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fits[1].descriptionnull
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_versionGNOM
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[0]X33
intensity_unitnull
experimental_mw170.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mwnull
porod_mw_errornull
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guinier_i0_errornull
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pddf_rg_errornull
guinier_rg5.1
guinier_rg_errornull
pddf_dmax19.0
pddf_dmax_errornull
porod_volume330.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_firstnull
guinier_point_lastnull
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDAD4_datloglog_img.png
symmetrynull
last_modified2023-05-25T11:22:16.556436+02:00
bragg_peak[]
manifest.json:36 field values
Field pathRaw value
codeSASDAD4
statussuccess
started_at2026-08-11T14:20:14.863748+00:00
finished_at2026-08-11T14:20:26.962068+00:00
source_last_modified2023-05-25T11:22:16.556436+02:00
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View the complete summary.json source
{
  "code": "SASDAD4",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "The C-terminal domain of the transcriptional corepressor CtBP is intrinsically unstructured.",
    "publication": {
      "title": "The C-terminal domain of the transcriptional corepressor CtBP is intrinsically unstructured.",
      "author_list": "Nardini M, Svergun D, Konarev PV, Spanò S, Fasano M, Bracco C, Pesce A, Donadini A, Cericola C, Secundo F, Luini A, Corda D, Bolognesi M",
      "journal": "Protein Sci",
      "doi": "10.1110/ps.062115406",
      "pmid": "16597837",
      "published_date": "2006 May"
    },
    "status": "released",
    "submitted_date": null,
    "released_date": "2015-07-07"
  },
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    "sample": {
      "molecule": [
        {
          "long_name": "full length CtBP3",
          "short_name": "full CtBP3",
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          "organism": null,
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          "oligomerization": "tetramer",
          "molecular_type": "protein",
          "uniprot_sequence": null,
          "mw": 42.0,
          "total_mw": 170.0,
          "number_molecules": 4,
          "complex_state": null,
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          "molecule_source": "biological",
          "molecule_description": null
        }
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      "name": "Full length GtBP3",
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      "absorbption": null,
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    },
    "contributor": [
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