SASDAE7

Structure of a complex between full length and truncated CS74L endolysin

Data type: SASBDB experimental data Status: Published Curve type: Merged Last updated: 2023-05-25T11:21:52.654103+02:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Structure of a complex between full length and truncated CS74L endolysin

Concentration1.0 – 6.8 Buffer / pH20 mM HEPES / 7.4
Experimental temperature20.0 Facility / beamlineDORIS III, DESY / EMBL X33
Wavelength0.15 nmExposure15.0 s × 8

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Endolysin CS74L
View sequence
MGSSHHHHHHSSGLVPRGSHMKIGIDMGHTLSGADYGVVGLRPESVLTREVGTKVIYKLQKLGHVVVNCTVDKASSVSESLYTRYYRANQANVDLFISIHFNATPGGTGTEVYTYAGRQLGEATRIRQEFKSLGLRDRGTKDGSGLAVIRNTKAKAMLVECCFCDNPNDMKLYNSESFSNAIVKGITGKLPNGESGNNNQGGNKVKAVVIYNEGADRRGAEYLADYLNCPTISNSRTFDYSCVEHVYAVGGKKEQYTKYLKTLLSGANRYDTMQQILNFINGGK
proteinClostridium phage phi8074-B1I1TJX31–264otherMolecule count —31.1 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
12143[3]0.162829–6.02412 1/nmHas uncertainty columnMissing 050sasbdb/entries/e7/sasdae7/source/SASDAE7.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)14.0—nm
i0Guinier50.3——
i0P(r)51.9——
mwExperimental38.4—kDa
mwGuinier I(0)38.4—kDa
mwPorod49.5—kDa
porod_volumePorod79.0—nm³
rgGuinier3.6—nm
rgP(r)3.8—nm

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name20 mM HEPESBuffer concentration—
pH7.4Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2011-03-17Storage / measurement temperature— / 20.0
Exposure time15.0Frames8
Wavelength0.15Sample-to-detector distance2.7
SourceX-ray synchrotronDetectorPilatus 1M-W
Facility / beamlineDORIS III, DESY / EMBL X33 · Hamburg, Germany
q range0.163 – 6.024Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:205 field values
Field pathRaw value
codeSASDAE7
statusPublished
type_of_curveMerged
angular_unit1/nm
project.titleCrystal Structure of the CTP1L Endolysin Reveals How Its Activity Is Regulated by a Secondary Translation Product.
project.publication.titleCrystal Structure of the CTP1L Endolysin Reveals How Its Activity Is Regulated by a Secondary Translation Product.
project.publication.author_listDunne M, Leicht S, Krichel B, Mertens HD, Thompson A, Krijgsveld J, Svergun DI, Gómez-Torres N, Garde S, Uetrecht C, Narbad A, Mayer MJ, Meijers R
project.publication.journalJ Biol Chem
project.publication.doi10.1074/jbc.M115.671172
project.publication.pmid26683375
project.publication.published_date2016 Mar 4
project.statusreleased
project.submitted_date2015-01-19
project.released_date2016-03-17
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experiment.instrument.detector.namePilatus 1M-W
experiment.instrument.detector.resolution0.172
experiment.instrument.nameDORIS III, DESY
experiment.instrument.cityHamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL X33
experiment.instrument.beam_geometry0.60
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameEndolysin CS74L
experiment.sample.molecule[0].short_nameCS74L
experiment.sample.molecule[0].sequenceMGSSHHHHHHSSGLVPRGSHMKIGIDMGHTLSGADYGVVGLRPESVLTREVGTKVIYKLQ KLGHVVVNCTVDKASSVSESLYTRYYRANQANVDLFISIHFNATPGGTGTEVYTYAGRQL GEATRIRQEFKSLGLRDRGTKDGSGLAVIRNTKAKAMLVECCFCDNPNDMKLYNSESFSN AIVKGITGKLPNGESGNNNQGGNKVKAVVIYNEGADRRGAEYLADYLNCPTISNSRTFDY SCVEHVYAVGGKKEQYTKYLKTLLSGANRYDTMQQILNFINGGK
experiment.sample.molecule[0].organismClostridium phage phi8074-B1
experiment.sample.molecule[0].uniprot_codeI1TJX3
experiment.sample.molecule[0].uniprot_range_first1
experiment.sample.molecule[0].uniprot_range_last264
experiment.sample.molecule[0].oligomerizationother
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMKIGIDMGHTLSGADYGVVGLRPESVLTREVGTKVIYKLQKLGHVVVNCTVDKASSVSES LYTRYYRANQANVDLFISIHFNATPGGTGTEVYTYAGRQLGEATRIRQEFKSLGLRDRGT KDGSGLAVIRNTKAKAMLVECCFCDNPNDMKLYNSESFSNAIVKGITGKLPNGESGNNNQ GGNKVKAVVIYNEGADRRGAEYLADYLNCPTISNSRTFDYSCVEHVYAVGGKKEQYTKYL KTLLSGANRYDTMQQILNFINGGK
experiment.sample.molecule[0].mw31.1
experiment.sample.molecule[0].total_mw31.1
experiment.sample.molecule[0].number_molecules0
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
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experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
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experiment.sample.buffer.pkanull
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experiment.sample.purity_methodnull
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experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
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experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameEMBL-Hamburg
experiment.contributor[0].affiliation[0].addressNotkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany
experiment.contributor[0].affiliation[0].full_nameEuropean Molecular Biology Laboratory (EMBL) - Hamburg outstation
experiment.contributor[0].affiliation[0].webpagehttp://www.embl-hamburg.de/index.php
experiment.contributor[0].contributor_nameHaydyn
experiment.contributor[0].contributor_surnameMertens
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2011-03-17
experiment.storage_temperaturenull
experiment.cell_temperature20.0
experiment.exposure_time15.0
experiment.number_of_frames8
experiment.wavelength0.15
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last_modified2023-05-25T11:21:52.654103+02:00
bragg_peak[]
manifest.json:36 field values
Field pathRaw value
codeSASDAE7
statussuccess
started_at2026-08-11T15:27:48.681066+00:00
finished_at2026-08-11T15:28:03.499559+00:00
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View the complete summary.json source
{
  "code": "SASDAE7",
  "status": "Published",
  "type_of_curve": "Merged",
  "angular_unit": "1/nm",
  "project": {
    "title": "Crystal Structure of the CTP1L Endolysin Reveals How Its Activity Is Regulated by a Secondary Translation Product.",
    "publication": {
      "title": "Crystal Structure of the CTP1L Endolysin Reveals How Its Activity Is Regulated by a Secondary Translation Product.",
      "author_list": "Dunne M, Leicht S, Krichel B, Mertens HD, Thompson A, Krijgsveld J, Svergun DI, Gómez-Torres N, Garde S, Uetrecht C, Narbad A, Mayer MJ, Meijers R",
      "journal": "J Biol Chem",
      "doi": "10.1074/jbc.M115.671172",
      "pmid": "26683375",
      "published_date": "2016 Mar 4"
    },
    "status": "released",
    "submitted_date": "2015-01-19",
    "released_date": "2016-03-17"
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        "resolution": 0.172
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      "name": "DORIS III, DESY",
      "city": "Hamburg",
      "country": "Germany",
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