SASDAG2

Lysozyme in sodium acetate

Data type: SASBDB experimental data Status: Published Curve type: Merged Last updated: 2023-05-25T11:21:52.654103+02:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Lysozyme in sodium acetate

Concentration— – 8.09 mg/mlBuffer / pH40 mM Sodium Acetate / 3.8
Experimental temperature10.0 Facility / beamlineDORIS III, DESY / EMBL X33
Wavelength0.15 nmExposure15.0 s × 8

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Lysozyme C
View sequence
>SP|P00698|LYSC_CHICKLYSOZYMECOS=GALLUSGALLUSGN=LYZPE=1SV=1MRSLLILVLCFLPLAALGKVFGRCELAAAMKRHGLDNYRGYSLGNWVCAAKFESNFNTQATNRNTDGSTDYGILQINSRWWCNDGRTPGSRNLCNIPCSALLSSDITASVNCAKKIVSDGNGMNAWVAWRNRCKGTDVQAWIRGCRL
proteinGallus gallusP00698—–—monomerMolecule count 116.24 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
13428[3]0.0871252–17.6902 1/nmHas uncertainty columnMissing 040sasbdb/entries/g2/sasdag2/source/SASDAG2.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)4.0—nm
i0Guinier12.29——
i0P(r)12.02——
mwExperimental11.0—kDa
mwGuinier I(0)11.0—kDa
porod_volumePorod20.7—nm³
rgGuinier1.45—nm
rgP(r)1.43—nm

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name40 mM Sodium AcetateBuffer concentration40.0 mM
pH3.8Additives—
Buffer description—
Purity measurement methodgel filtrationExtinction coefficient26.53
Absorbance—Scattering contrast3.047
Specific volume / dry volume0.7402 / 19648Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2012-09-21Storage / measurement temperature10.0 / 10.0
Exposure time15.0Frames8
Wavelength0.15Sample-to-detector distance2.7
SourceX-ray synchrotronDetectorPilatus 1M-W
Facility / beamlineDORIS III, DESY / EMBL X33 · Hamburg, Germany
q range0.087 – 17.69Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:178 field values
Field pathRaw value
codeSASDAG2
statusPublished
type_of_curveMerged
angular_unit1/nm
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project.publicationnull
project.statusreleased
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experiment.instrument.detector.namePilatus 1M-W
experiment.instrument.detector.resolution0.172
experiment.instrument.nameDORIS III, DESY
experiment.instrument.cityHamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL X33
experiment.instrument.beam_geometry0.60
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameLysozyme C
experiment.sample.molecule[0].short_nameLys
experiment.sample.molecule[0].sequence>sp|P00698|LYSC_CHICK Lysozyme C OS=Gallus gallus GN=LYZ PE=1 SV=1 MRSLLILVLCFLPLAALGKVFGRCELAAAMKRHGLDNYRGYSLGNWVCAAKFESNFNTQA TNRNTDGSTDYGILQINSRWWCNDGRTPGSRNLCNIPCSALLSSDITASVNCAKKIVSDG NGMNAWVAWRNRCKGTDVQAWIRGCRL
experiment.sample.molecule[0].organismGallus gallus
experiment.sample.molecule[0].uniprot_codeP00698
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
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experiment.sample.molecule[0].mw16.24
experiment.sample.molecule[0].total_mw14.313
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_statenull
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name40 mM Sodium Acetate
experiment.sample.buffer.concentration_unitmM
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentration40.0
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph3.8
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodgel filtration
experiment.sample.nameLysozyme in sodium acetate
experiment.sample.ext_coefficient26.53
experiment.sample.contrast3.047
experiment.sample.specific_vol0.7402
experiment.sample.dry_vol19648
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameEMBL-Hamburg
experiment.contributor[0].affiliation[0].addressNotkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany
experiment.contributor[0].affiliation[0].full_nameEuropean Molecular Biology Laboratory (EMBL) - Hamburg outstation
experiment.contributor[0].affiliation[0].webpagehttp://www.embl-hamburg.de/index.php
experiment.contributor[0].contributor_nameDarja
experiment.contributor[0].contributor_surnameRuskule
experiment.contributor[0].orcidnull
experiment.concentration_methodnanodrop
experiment.concentration_unitmg/ml
experiment.date2012-09-21
experiment.storage_temperature10.0
experiment.cell_temperature10.0
experiment.exposure_time15.0
experiment.number_of_frames8
experiment.wavelength0.15
experiment.sample_detector_distance2.7
experiment.concentration_minnull
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fits[0].models[0].symmetryP1
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descriptionnull
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tags[1]X33
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guinier_i0_mw_errornull
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pddf_i012.02
pddf_i0_errornull
guinier_i012.29
guinier_i0_errornull
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pddf_rg_errornull
guinier_rg1.45
guinier_rg_errornull
pddf_dmax4.0
pddf_dmax_errornull
porod_volume20.7
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first177
guinier_point_last294
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pddf_point_lastnull
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intensities_log_log_plotSASDAG2_datloglog_img.png
symmetrynull
last_modified2023-05-25T11:21:52.654103+02:00
bragg_peak[]
manifest.json:36 field values
Field pathRaw value
codeSASDAG2
statussuccess
started_at2026-08-11T13:37:56.248268+00:00
finished_at2026-08-11T13:38:07.107815+00:00
source_last_modified2023-05-25T11:21:52.654103+02:00
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View the complete summary.json source
{
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  "status": "Published",
  "type_of_curve": "Merged",
  "angular_unit": "1/nm",
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    "title": "Standard proteins",
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    "status": "released",
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      },
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      "name": "Lysozyme in sodium acetate",
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      "contrast": 3.047,
      "specific_vol": 0.7402,
      "dry_vol": 19648,
      "absorbption": null,
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    "contributor": [
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    "concentration_method": "nanodrop",
    "concentration_unit": "mg/ml",
    "date": "2012-09-21",
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View the complete manifest.json source
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