SASDAH4

DH-PH

Data type: SASBDB experimental data Status: Published Curve type: Merged Last updated: 2023-05-25T11:22:16.556436+02:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · DH-PH

Concentration0.86 – 4.7 mg/mlBuffer / pH50 mM Tris-HCL 150 mM NaCl 1.0 mM DTT / 7.5
Experimental temperature10.0 Facility / beamlineDORIS III, DESY / EMBL X33
Wavelength— nmExposure— s × —

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
DH-PH module of PDZRhoGEF
View sequence
QNWQHTVGKDVVAGLTQREIDRQEVINELFVTEASHLRTLRVLDLIFYQRMKKENLMPREELARLFPNLPELIEIHNSWCEAMKKLREEGPIIKEISDLMLARFDGPAREELQQVAAQFCSYQSIALELIKTKQRKESRFQLFMQEAESHPQCRRLQLRDLIISEMQRLTKYPLLLESIIKHTEGGTSEHEKLCRARDQCREILKYVNEAVKQTENRHRLEGYQKRLDATALERASNPLAAEFKSLDLTTRKMIHEGPLTWRISKDKTLDLHVLLLEDLLVLLQKQDEKLLLKCTFSPVLKLNAVLIRSVATDKRAFFIICTSKLGPPQIYELVALTSSDKNTWMELLEEAVRNA
proteinHomo sapiens——–—monomerMolecule count 141.0 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
11994[3]0.2295–4.816 1/nmHas uncertainty columnMissing 00sasbdb/entries/h4/sasdah4/source/SASDAH4.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)9.0—nm
i0Guinier0.378——
mwExperimental40.0—kDa
porod_volumePorod60.0—nm³
rgGuinier2.9—nm

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name50 mM Tris-HCL 150 mM NaCl 1.0 mM DTTBuffer concentration—
pH7.5Additives150 mM NaCl, 1.0 mM DTT
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2008-10-30Storage / measurement temperature10.0 / 10.0
Exposure time—Frames—
Wavelength—Sample-to-detector distance—
SourceX-ray synchrotronDetectorMAR 345 Image Plate
Facility / beamlineDORIS III, DESY / EMBL X33 · Hamburg, Germany
q range0.23 – 4.816Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:179 field values
Field pathRaw value
codeSASDAH4
statusPublished
type_of_curveMerged
angular_unit1/nm
project.titleThe solution structure and dynamics of the DH-PH module of PDZRhoGEF in isolation and in complex with nucleotide-free RhoA.
project.publication.titleThe solution structure and dynamics of the DH-PH module of PDZRhoGEF in isolation and in complex with nucleotide-free RhoA.
project.publication.author_listCierpicki T, Bielnicki J, Zheng M, Gruszczyk J, Kasterka M, Petoukhov M, Zhang A, Fernandez EJ, Svergun DI, Derewenda U, Bushweller JH, Derewenda ZS
project.publication.journalProtein Sci
project.publication.doi10.1002/pro.219
project.publication.pmid19670212
project.publication.published_date2009 Oct
project.statusreleased
project.submitted_datenull
project.released_date2015-07-07
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experiment.instrument.detector.typenull
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experiment.instrument.detector.resolutionnull
experiment.instrument.nameDORIS III, DESY
experiment.instrument.cityHamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL X33
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
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experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
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experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameDH-PH module of PDZRhoGEF
experiment.sample.molecule[0].short_nameDH-PH
experiment.sample.molecule[0].sequenceQNWQHTVGKDVVAGLTQREIDRQEVINELFVTEASHLRTLRVLDLIFYQRMKKENLMPREELARLFPNLPELIEIHNSWC EAMKKLREEGPIIKEISDLMLARFDGPAREELQQVAAQFCSYQSIALELIKTKQRKESRFQLFMQEAESHPQCRRLQLRD LIISEMQRLTKYPLLLESIIKHTEGGTSEHEKLCRARDQCREILKYVNEAVKQTENRHRLEGYQKRLDATALERASNPLA AEFKSLDLTTRKMIHEGPLTWRISKDKTLDLHVLLLEDLLVLLQKQDEKLLLKCTFSPVLKLNAVLIRSVATDKRAFFII CTSKLGPPQIYELVALTSSDKNTWMELLEEAVRNA
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequencenull
experiment.sample.molecule[0].mw41.0
experiment.sample.molecule[0].total_mw41.0
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_statenull
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experiment.sample.molecule[0].molecule_sourcebiological
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experiment.sample.buffer.name50 mM Tris-HCL 150 mM NaCl 1.0 mM DTT
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additive150 mM NaCl, 1.0 mM DTT
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameDH-PH
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameEMBL-Hamburg
experiment.contributor[0].affiliation[0].addressNotkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany
experiment.contributor[0].affiliation[0].full_nameEuropean Molecular Biology Laboratory (EMBL) - Hamburg outstation
experiment.contributor[0].affiliation[0].webpagehttp://www.embl-hamburg.de/index.php
experiment.contributor[0].contributor_nameMaxim
experiment.contributor[0].contributor_surnamePetoukhov
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitmg/ml
experiment.date2008-10-30
experiment.storage_temperature10.0
experiment.cell_temperature10.0
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i0_calibration_standardBSA
descriptionnull
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guinier_point_last96
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symmetrynull
last_modified2023-05-25T11:22:16.556436+02:00
bragg_peak[]
manifest.json:36 field values
Field pathRaw value
codeSASDAH4
statussuccess
started_at2026-08-11T14:23:49.205108+00:00
finished_at2026-08-11T14:23:58.330572+00:00
source_last_modified2023-05-25T11:22:16.556436+02:00
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View the complete summary.json source
{
  "code": "SASDAH4",
  "status": "Published",
  "type_of_curve": "Merged",
  "angular_unit": "1/nm",
  "project": {
    "title": "The solution structure and dynamics of the DH-PH module of PDZRhoGEF in isolation and in complex with nucleotide-free RhoA.",
    "publication": {
      "title": "The solution structure and dynamics of the DH-PH module of PDZRhoGEF in isolation and in complex with nucleotide-free RhoA.",
      "author_list": "Cierpicki T, Bielnicki J, Zheng M, Gruszczyk J, Kasterka M, Petoukhov M, Zhang A, Fernandez EJ, Svergun DI, Derewenda U, Bushweller JH, Derewenda ZS",
      "journal": "Protein Sci",
      "doi": "10.1002/pro.219",
      "pmid": "19670212",
      "published_date": "2009 Oct"
    },
    "status": "released",
    "submitted_date": null,
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