SASDAL5

Clostridium difficile bacteriophage 27 endolysin dimer, CD27L

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Merged 最后更新:2023-05-25T11:21:52.654103+02:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Clostridium difficile bacteriophage 27 endolysin dimer, CD27L

浓度0.9 – 4.0 缓冲液 / pH20 mM HEPES 150 mM NaCl / 7.5
实验温度10.0 设备 / 束线DORIS III, DESY / EMBL X33
波长0.15 nm曝光15.0 s × 8

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Clostridium difficile bacteriophage 27 endolysin
查看序列
MGSSHHHHHHSSGLVPRGSHMKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADTFRKEGHKVDVIISPEKQFKTKNEEKSYKIPRVNSGGYDLLIELHLNASNGQGKGSEVLYYSNKGLEYATRICDKLGTVFKNRGAKLDKRLYILNSSKPTAVLIESFFCDNKEDYDKAKKLGHEGIAKLIVEGVLNKNINNEGVKQMYKHTIVYDGEVDKISATVVGWGYNDGKILICDIKDYVPGQTQNLYVVGGGACEKISSITKEKFIMIKGNDRFDTLYKALDFIN
proteinClostridioides difficile——–—dimer分子数 232.1 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
12316[3]0.129187–6.34517 1/nm含误差列缺失 059sasbdb/entries/l5/sasdal5/source/SASDAL5.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)10.6—nm
i0Guinier45.6——
i0P(r)44.0——
mwExperimental42.1—kDa
mwGuinier I(0)42.1—kDa
mwPorod45.1—kDa
porod_volumePorod72.15—nm³
rgGuinier3.3—nm
rgP(r)3.3—nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

拟合 1 · —

χ²:2.4649CorMap p:0.0单位:1/A
模型名称 / 类型软件对称性模型 MWPDB 声明来源文件
1—atomicOLIGOMER —64.24—模型 图片
2—atomicOLIGOMER —64.36—模型 图片
3—atomicOLIGOMER —41.53—模型 图片
4—atomicOLIGOMER —42.2—模型 图片
5—atomicOLIGOMER —18.82—模型 图片
6—atomicOLIGOMER —18.82—模型 图片
7—atomicOLIGOMER —32.13—模型 图片
8—atomicOLIGOMER —19.95—模型 图片
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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称20 mM HEPES 150 mM NaCl缓冲液浓度—
pH7.5添加剂150 mM NaCl
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2011-03-17储存 / 测量温度10.0 / 10.0
曝光时间15.0帧数8
波长0.15样品-探测器距离2.7
光源X-ray synchrotron探测器Pilatus 1M-W
机构 / 束线DORIS III, DESY / EMBL X33 · Hamburg, Germany
q 范围0.129 – 6.345样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
curvesasbdb/entries/l5/sasdal5/source/SASDAL5.datdownloaded104472a38efce15ffe5f131f77c406b209b87839399e0dc14fda4a2cd9c1d44dafc885下载查看原文件源站
full_entry_zipsasbdb/entries/l5/sasdal5/source/SASDAL5.zipdownloaded368324ee4de0eec5fe78a5b82650aa0144252eaf82ec5f37b4a5d8c06b67df74beafef下载查看原文件源站
pddfsasbdb/entries/l5/sasdal5/source/SASDAL5.outdownloaded36698f5799a317d0b89146e280dfc09b320a926cdb23d123b4147149ce45b0451f0b5下载查看原文件源站
sascifsasbdb/entries/l5/sasdal5/source/SASDAL5.sascifdownloaded21030449bec08a38b1d36425bc75ed48b3a80b5ed48396f04bcf1f8b220c118df2dc451下载查看原文件源站
summarysasbdb/entries/l5/sasdal5/source/summary.jsondownloaded10572f53cbb7fbcd632126a9cf3217b0df1284343c09d7f0b44de465460b23a28b98d下载查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(11 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:247 个字段值
字段路径原始值
codeSASDAL5
statusPublished
type_of_curveMerged
angular_unit1/nm
project.titleThe CD27L and CTP1L endolysins targeting Clostridia contain a built-in trigger and release factor.
project.publication.titleThe CD27L and CTP1L endolysins targeting Clostridia contain a built-in trigger and release factor.
project.publication.author_listDunne M, Mertens HD, Garefalaki V, Jeffries CM, Thompson A, Lemke EA, Svergun DI, Mayer MJ, Narbad A, Meijers R
project.publication.journalPLoS Pathog
project.publication.doi10.1371/journal.ppat.1004228
project.publication.pmid25058163
project.publication.published_date2014 Jul
project.statusreleased
project.submitted_datenull
project.released_date2014-07-24
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDAL5.out
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sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDAL5.sascif
experiment.instrument.detector.typeCustom-built
experiment.instrument.detector.namePilatus 1M-W
experiment.instrument.detector.resolution0.172
experiment.instrument.nameDORIS III, DESY
experiment.instrument.cityHamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL X33
experiment.instrument.beam_geometry0.60
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameClostridium difficile bacteriophage 27 endolysin
experiment.sample.molecule[0].short_nameCD27L
experiment.sample.molecule[0].sequenceMGSSHHHHHHSSGLVPRGSHMKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADTFRKEGHKVDVIIsPEKQFKTKNEEKSYKIPRVNSGGYDLL IELHLNASNGQGKGSEVLYYSNKGLEYATRICDKLGTVFKNRGAKLDKRLYILNSSKPTAVLIESFFCDNKEDYDKAKKLGHEGIAKLIVEGVLNKNINN EGVKQMYKHTIVYDGEVDKISATVVGWGYNDGKILICDIKDYVPGQTQNLYVVGGGACEKISSITKEKFIMIKGNDRFDTLYKALDFIN
experiment.sample.molecule[0].organismClostridioides difficile
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequencenull
experiment.sample.molecule[0].mw32.1
experiment.sample.molecule[0].total_mw64.2
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_statenull
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name20 mM HEPES 150 mM NaCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additive150 mM NaCl
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameClostridium difficile bacteriophage 27 endolysin dimer, CD27L
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameEMBL-Hamburg
experiment.contributor[0].affiliation[0].addressNotkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany
experiment.contributor[0].affiliation[0].full_nameEuropean Molecular Biology Laboratory (EMBL) - Hamburg outstation
experiment.contributor[0].affiliation[0].webpagehttp://www.embl-hamburg.de/index.php
experiment.contributor[0].contributor_nameHaydyn
experiment.contributor[0].contributor_surnameMertens
experiment.contributor[0].orcidnull
experiment.concentration_methodA280
experiment.concentration_unitnull
experiment.date2011-03-17
experiment.storage_temperature10.0
experiment.cell_temperature10.0
experiment.exposure_time15.0
experiment.number_of_frames8
experiment.wavelength0.15
experiment.sample_detector_distance2.7
experiment.concentration_min0.9
experiment.concentration_max4.0
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.129
experiment.s_max6.345
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].chi_square_value2.4649
fits[0].p_value0.0
fits[0].fit_residual_plotSASDAL5_fit1_fitresiduals_img.png
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fits[0].descriptionnull
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_versionnull
i0_calibration_standardBSA
descriptionnull
experiment_descriptionnull
tags[0]X33
intensity_unitnull
experimental_mw42.1
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porod_mw45.1
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guinier_i045.6
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pddf_rg_errornull
guinier_rg3.3
guinier_rg_errornull
pddf_dmax10.6
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porod_volume72.15
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estimated_volumenull
estimated_volume_errornull
guinier_point_first1
guinier_point_last100
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i0_calibration_standard_datanull
intensities_log_log_plotSASDAL5_datloglog_img.png
symmetrynull
last_modified2023-05-25T11:21:52.654103+02:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDAL5
statussuccess
started_at2026-08-11T14:50:07.401459+00:00
finished_at2026-08-11T14:50:18.357184+00:00
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查看完整 summary.json 原文
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    "title": "The CD27L and CTP1L endolysins targeting Clostridia contain a built-in trigger and release factor.",
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      "title": "The CD27L and CTP1L endolysins targeting Clostridia contain a built-in trigger and release factor.",
      "author_list": "Dunne M, Mertens HD, Garefalaki V, Jeffries CM, Thompson A, Lemke EA, Svergun DI, Mayer MJ, Narbad A, Meijers R",
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查看完整 manifest.json 原文
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