SASDAL6

Wild-type chalcone isomerase, ligand-free

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Extrapolated to infinite dilution 最后更新:2023-06-21T07:56:39.492710+02:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Wild-type chalcone isomerase, ligand-free

浓度1.38 – 15.3 缓冲液 / pH50 mM sodium phosphate / 6.8
实验温度10.0 设备 / 束线PETRA III / EMBL P12
波长0.124 nm曝光0.05 s × 20

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Bacterial chalcone isomerase
查看序列
>TR|V9P0A9|V9P0A9_EUBRACHALCONEISOMERASEOS=EUBACTERIUMRAMULUSGN=TAIPE=1SV=1MADFKFEPMRSLIYVDCVSEDYRPKLQRWIYKVHIPDSISQFEPYVTKYAFYPSFPIPPQGDRFGYARMQLTEHHWLVSDLDPRLEIKAIAETFPMDVLVWQGQIPAAAHTDAQIDSDGDAGNAARKSNNAEGNPFIFAFLPMWWEKDLKGKGRTIEDGANYRFNMTIGFPEGVDKAEGEKWLFEKVVPILQAAPECTRVLASAVKKDINGCVMDWVLEIWFENQSGWYKVMVDDMKALEKPSWAQQDAFPFLKPYHNVCSAAVADYTPSNNLANYRGYITMR
proteinEubacterium ramulusV9P0A9—–—hexamer分子数 632.4 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
11667[3]0.0754087–4.45777 1/nm含误差列缺失 01sasbdb/entries/l6/sasdal6/source/SASDAL6.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)13.00.5nm
i0Guinier32346.5—arbitrary
i0P(r)25450.0—arbitrary
mwExperimental190.0—kDa
mwGuinier I(0)190.0—kDa
mwPorod190.0—kDa
porod_volumePorod320.0—nm³
rgGuinier3.97—nm
rgP(r)3.7—nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

该条目没有来源拟合记录。

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称50 mM sodium phosphate缓冲液浓度—
pH6.8添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度3.047
比体积 / 干体积— / —混合物 / 氘代False / —

采集条件与仪器

测量日期2013-09-23储存 / 测量温度10.0 / 10.0
曝光时间0.05帧数20
波长0.124样品-探测器距离3.1
光源X-ray synchrotron探测器Pilatus 2M
机构 / 束线PETRA III / EMBL P12 · DESY; Hamburg, Germany
q 范围0.075 – 4.458样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(2 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:134 个字段值
字段路径原始值
codeSASDAL6
statusPublished
type_of_curveExtrapolated to infinite dilution
angular_unit1/nm
project.titleStructure and catalytic mechanism of the evolutionarily unique bacterial chalcone isomerase.
project.publication.titleStructure and catalytic mechanism of the evolutionarily unique bacterial chalcone isomerase.
project.publication.author_listThomsen M, Tuukkanen A, Dickerhoff J, Palm GJ, Kratzat H, Svergun DI, Weisz K, Bornscheuer UT, Hinrichs W
project.publication.journalActa Crystallogr D Biol Crystallogr
project.publication.doi10.1107/S1399004715001935
project.publication.pmid25849401
project.publication.published_date2015 Apr
project.statusreleased
project.submitted_date2015-07-21
project.released_date2017-11-06
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experiment.instrument.namePETRA III
experiment.instrument.cityDESY; Hamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL P12
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experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameBacterial chalcone isomerase
experiment.sample.molecule[0].short_nameCHI
experiment.sample.molecule[0].sequence>tr|V9P0A9|V9P0A9_EUBRA Chalcone isomerase OS=Eubacterium ramulus GN=tai PE=1 SV=1 MADFKFEPMRSLIYVDCVSEDYRPKLQRWIYKVHIPDSISQFEPYVTKYAFYPSFPIPPQ GDRFGYARMQLTEHHWLVSDLDPRLEIKAIAETFPMDVLVWQGQIPAAAHTDAQIDSDGD AGNAARKSNNAEGNPFIFAFLPMWWEKDLKGKGRTIEDGANYRFNMTIGFPEGVDKAEGE KWLFEKVVPILQAAPECTRVLASAVKKDINGCVMDWVLEIWFENQSGWYKVMVDDMKALE KPSWAQQDAFPFLKPYHNVCSAAVADYTPSNNLANYRGYITMR
experiment.sample.molecule[0].organismEubacterium ramulus
experiment.sample.molecule[0].uniprot_codeV9P0A9
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationhexamer
experiment.sample.molecule[0].molecular_typeprotein
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experiment.sample.molecule[0].mw32.4
experiment.sample.molecule[0].total_mw194.4
experiment.sample.molecule[0].number_molecules6
experiment.sample.molecule[0].complex_statenull
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name50 mM sodium phosphate
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph6.8
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameWild-type chalcone isomerase, ligand-free
experiment.sample.ext_coefficientnull
experiment.sample.contrast3.047
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixtureFalse
experiment.contributor[0].affiliation[0].short_nameEMBL-Hamburg
experiment.contributor[0].affiliation[0].addressNotkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany
experiment.contributor[0].affiliation[0].full_nameEuropean Molecular Biology Laboratory (EMBL) - Hamburg outstation
experiment.contributor[0].affiliation[0].webpagehttp://www.embl-hamburg.de/index.php
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experiment.contributor[0].contributor_surnameTuukkanen
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2013-09-23
experiment.storage_temperature10.0
experiment.cell_temperature10.0
experiment.exposure_time0.05
experiment.number_of_frames20
experiment.wavelength0.124
experiment.sample_detector_distance3.1
experiment.concentration_min1.38
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experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.075
experiment.s_max4.458
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experiment.seccolumnnull
fits[]
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardBSA
descriptionnull
experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw190.0
experimental_mw_errornull
guinier_i0_mw190.0
guinier_i0_mw_errornull
porod_mw190.0
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guinier_i032346.5
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pddf_rg3.7
pddf_rg_errornull
guinier_rg3.97
guinier_rg_errornull
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pddf_dmax_error0.5
porod_volume320.0
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estimated_volume_errornull
guinier_point_first1
guinier_point_last73
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDAL6_datloglog_img.png
symmetrynull
last_modified2023-06-21T07:56:39.492710+02:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDAL6
statussuccess
started_at2026-08-11T15:11:43.772734+00:00
finished_at2026-08-11T15:11:52.763729+00:00
source_last_modified2023-06-21T07:56:39.492710+02:00
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查看完整 summary.json 原文
{
  "code": "SASDAL6",
  "status": "Published",
  "type_of_curve": "Extrapolated to infinite dilution",
  "angular_unit": "1/nm",
  "project": {
    "title": "Structure and catalytic mechanism of the evolutionarily unique bacterial chalcone isomerase.",
    "publication": {
      "title": "Structure and catalytic mechanism of the evolutionarily unique bacterial chalcone isomerase.",
      "author_list": "Thomsen M, Tuukkanen A, Dickerhoff J, Palm GJ, Kratzat H, Svergun DI, Weisz K, Bornscheuer UT, Hinrichs W",
      "journal": "Acta Crystallogr D Biol Crystallogr",
      "doi": "10.1107/S1399004715001935",
      "pmid": "25849401",
      "published_date": "2015 Apr"
    },
    "status": "released",
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    "released_date": "2017-11-06"
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}
查看完整 manifest.json 原文
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